Feature selection represents an essential pre-processing step for a wide range of Machine Learning approaches. Datasets typically contain irrelevant features that may negatively affect the classifier ...performance. A feature selector can reduce the number of these features and maximise the classifier accuracy. This paper proposes a Dynamic Butterfly Optimization Algorithm (DBOA) as an improved variant to Butterfly Optimization Algorithm (BOA) for feature selection problems. BOA represents one of the most recently proposed optimization algorithms. BOA has demonstrated its ability to solve different types of problems with competitive results compared to other optimization algorithms. However, the original BOA algorithm has problems when optimising high-dimensional problems. Such issues include stagnation into local optima and lacking solutions diversity during the optimization process. To alleviate these weaknesses of the original BOA, two significant improvements are introduced in the original BOA: the development of a Local Search Algorithm Based on Mutation (LSAM) operator to avoid local optima problem and the use of LSAM to improve BOA solutions diversity. To demonstrate the efficiency and superiority of the proposed DBOA algorithm, 20 benchmark datasets from the UCI repository are employed. The classification accuracy, the fitness values, the number of selected features, the statistical results, and convergence curves are reported for DBOA and its competing algorithms. These results demonstrate that DBOA significantly outperforms the comparative algorithms on the majority of the used performance metrics.
Triple negative breast cancer (TNBC) is known as hot immunogenic tumor. Yet, it is one of the most aggressive BC subtypes. TNBC evolve several tactics to evade the immune surveillance phenomena, one ...of which is shedding of natural killer (NK) cells activating immune ligands such as MICA/B and/or by inducing the expression of the immune checkpoints such as PD-L1 and B7-H4. MALAT-1 is an oncogenic lncRNA. MALAT-1 immunogenic profile is not well investigated.
The study aims at exploring the immunogenic role of MALAT-1 in TNBC patients and cell lines and to identify its molecular mechanism in altering both innate and adaptive immune cells present at the tumor microenvironment of TNBC
BC patients (n = 35) were recruited. Primary NK cells and cytotoxic T lymphocytes were isolated from normal individuals using the negative selection method. MDA-MB-231 cells were cultured and transfected by several oligonucleotides by lipofection technique. Screening of ncRNAs was performed using q-RT-PCR. Immunological functional analysis experiments were performed upon co-culturing primary natural killer cells and cytotoxic T lymphocytes using LDH assay. Bioinformatics analysis was performed to identify potential microRNAs targeted by MALAT-1.
MALAT-1 expression was significantly upregulated in BC patinets with a profound expression in TNBC patients compared to their normal counterparts. Correlation analysis revealed a positive correlation between MALAT-1, tumor size and lymph node metastasis. Knocking down of MALAT-1 in MDA-MB-231 cells resulted in a significant induction of MICA/B, repression of PD-L1 and B7-H4 expression levels. Enhancement of cytotoxic activity of co-cultured NK and CD8+ cells with MALAT-1 siRNAs transfected MDA-MB-231 cells. In silico analysis revealed that miR-34a and miR-17–5p are potential targets to MALAT-1; accordingly, they were found to be downregulated in BC patients. Forcing the expression of miR-34a in MDA-MB-231 cells resulted in a significant induction in MICA/B levels. Ectopic expression of miR-17–5p in MDA-MB-231 cells significantly repressed the expression of PD-L1 and B7-H4 checkpoints. Validations of MALAT-1/miR-34a" and "MALAT-1/miR-17–5p axes were performed by a series of co-transfections and functional assessment of cytotoxic profile of primary immune cells.
This study proposes a novel epigenetic alteration exerted by TNBC cells mainly by inducing the expression of MALAT-1 lncRNA. MALAT-1 mediates innate and adaptive immune suppression events partially via targeting miR-34a/MICA/B and miR-175p/PD-L1/B7-H4 axes in TNBC patients and cell lines.
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Abstract Objective The purpose of this study was to measure the extent to which geographic residency status and the social environment are associated with disease stage at diagnosis, receipt of ...treatment, and five-year survival for patients diagnosed with non-small cell lung cancer (NSCLC). Methods and materials This study was a retrospective cohort study of the Georgia Comprehensive Cancer Registry (GCCR) for incident cases of NSCLC diagnosed in the state. Multilevel logistic models were employed for five outcome variables: unstaged and late stage disease at diagnosis; receipt of treatment (surgery, chemotherapy, and radiation); and survival following diagnosis. The social and geographical variables of interest were census tract (CT) poverty level, CT-level educational attainment, and CT-level geographic residency status. Results Compared to urban residents, rural and suburban residents had increased odds of unstaged disease (suburban OR = 1.23, 95% CI: 1.11–1.37; rural OR = 1.63, 95% CI: 1.45–1.83). In this study, rural participants had lower odds of receiving radiotherapy (OR = 0.89, 95% CI: 0.82–0.96) and chemotherapy (OR = 0.92, 95% CI: 0.85–0.99). Living in CTs with lower educational levels was associated with decreasing odds of receiving both surgery (lowest educational level OR = 0.67, 95% CI: 0.59–0.75) and chemotherapy (lowest educational level OR = 0.74, 95% CI: 0.68–0.81). Living in areas with higher concentration of deprivation (high level of deprivation HR = 1.04, 95% CI: 1.01–1.09) and lower levels of education (lowest educational level HR = 1.12, 95% CI: 1.07–1.17) was associated with poorer survival. Rural residents did not show poorer survival when treatment was controlled and they even presented a lower risk of death for early stage disease (HR = 0.90, 95% CI: 0.82–0.99). Conclusion This study concludes that where NSCLC patients live can, to some extent, explain treatment and prognostic disparities. Public health practitioners and policy makers should be cognizant of the importance of where people live and shift their efforts to improve lung cancer outcomes in rural areas and neighborhoods with concentrated poverty.
Investigating aircraft flight dynamics often requires dynamic wind tunnel testing. This paper proposes a non-contact, off-board instrumentation method using vision-based techniques. The method ...utilises a sequential process of Harris corner detection, Kanade–Lucas–Tomasi tracking, and quaternions to identify the Euler angles from a pair of cameras, one with a side view and the other with a top view. The method validation involves simulating a 3D CAD model for rotational motion with a single degree-of-freedom. The numerical analysis quantifies the results, while the proposed approach is analysed analytically. This approach results in a 45.41% enhancement in accuracy over an earlier direction cosine matrix method. Specifically, the quaternion-based method achieves root mean square errors of 0.0101 rad/s, 0.0361 rad/s, and 0.0036 rad/s for the dynamic measurements of roll rate, pitch rate, and yaw rate, respectively. Notably, the method exhibits a 98.08% accuracy for the pitch rate. These results highlight the performance of quaternion-based attitude estimation in dynamic wind tunnel testing. Furthermore, an extended Kalman filter is applied to integrate the generated on-board instrumentation data (inertial measurement unit, potentiometer gimbal) and the results of the proposed vision-based method. The extended Kalman filter state estimation achieves root mean square errors of 0.0090 rad/s, 0.0262 rad/s, and 0.0034 rad/s for the dynamic measurements of roll rate, pitch rate, and yaw rate, respectively. This method exhibits an improved accuracy of 98.61% for the estimation of pitch rate, indicating its higher efficiency over the standalone implementation of the direction cosine method for dynamic wind tunnel testing.
A mixture of two triamines, one diamine, 2‐formylpyridine and a ZnII salt was found to self‐sort, cleanly producing a mixture of three different tetrahedral cages. Each cage bound one of three guests ...selectively. These guests could be released in a specific sequence following the addition of 4‐methoxyaniline, which reacted with the cages, opening each in turn and releasing its guest. The system here described thus behaved in an organized way in three distinct contexts: cage formation, guest encapsulation, and guest release. Such behavior could be used in the context of a more complex system, where released guests serve as signals to other chemical actors.
Well sorted: Three discrete metal–organic cages were prepared through the one‐pot reaction of five subcomponents, that is, three amines, 2‐formylpyridine, and a ZnII salt. Each cage selectively accommodates a single guest within a mixture of three guests, and each guest can be sequentially released following the addition of a chemical stimulus.
Abstract Complex permittivity of Corn, Jowar, Ashoka and Banana plant leaves was measured using a Vector Network Analyzer (Anritsu Shockline Model-MS46322A) over 500 MHz to 15 GHz frequency range, at ...different moisture contents. The dielectric constant ( $$\varepsilon$$ ε ′) and dielectric loss ( $$\varepsilon$$ ε ″) of the leaves decrease with decrease in moisture content over this frequency range. For the leaves having moisture content less than certain critical moisture level ( CML ), the values of $$\varepsilon$$ ε ′ and $$\varepsilon$$ ε ” remain almost constant with frequency variation. $$\varepsilon$$ ε ″ does not increase appreciably with the increase in moisture content up to CML . CML is found to vary for different types of leaves. At higher moisture level, $$\varepsilon$$ ε ′ and $$\varepsilon$$ ε ″ exhibit frequency dependence. Above certain moisture level in the leaves, $$\varepsilon$$ ε ″ starts to increase slowly with the increase in frequency above 3 GHz approaching towards the dielectric relaxation of water. The values of $$\varepsilon$$ ε ′ and $$\varepsilon$$ ε ″ were also measured for the extract of leaves and compared with the values calculated using Stogryn equations for the same salinity, and the results agree with the calculated values. The measured values of $$\varepsilon$$ ε ′ and $$\varepsilon$$ ε ″ for the leaves were compared with the values calculated using Debye–Cole dual dispersion dielectric model and are found to match very well.
Heterozygous germ-line mutations in the bone morphogenetic protein type-II receptor (BMPR-II) gene underlie heritable pulmonary arterial hypertension (HPAH). Although inflammation promotes PAH, the ...mechanisms by which inflammation and BMPR-II dysfunction conspire to cause disease remain unknown. Here we identify that tumour necrosis factor-α (TNFα) selectively reduces BMPR-II transcription and mediates post-translational BMPR-II cleavage via the sheddases, ADAM10 and ADAM17 in pulmonary artery smooth muscle cells (PASMCs). TNFα-mediated suppression of BMPR-II subverts BMP signalling, leading to BMP6-mediated PASMC proliferation via preferential activation of an ALK2/ACTR-IIA signalling axis. Furthermore, TNFα, via SRC family kinases, increases pro-proliferative NOTCH2 signalling in HPAH PASMCs with reduced BMPR-II expression. We confirm this signalling switch in rodent models of PAH and demonstrate that anti-TNFα immunotherapy reverses disease progression, restoring normal BMP/NOTCH signalling. Collectively, these findings identify mechanisms by which BMP and TNFα signalling contribute to disease, and suggest a tractable approach for therapeutic intervention in PAH.
CONTEXT Solid organ transplant recipients have elevated cancer risk due to immunosuppression and oncogenic viral infections. Because most prior research has concerned kidney recipients, large studies ...that include recipients of differing organs can inform cancer etiology. OBJECTIVE To describe the overall pattern of cancer following solid organ transplantion. DESIGN, SETTING, AND PARTICIPANTS Cohort study using linked data on solid organ transplant recipients from the US Scientific Registry of Transplant Recipients (1987-2008) and 13 state and regional cancer registries. MAIN OUTCOME MEASURES Standardized incidence ratios (SIRs) and excess absolute risks (EARs) assessing relative and absolute cancer risk in transplant recipients compared with the general population. RESULTS The registry linkages yielded data on 175 732 solid organ transplants (58.4% for kidney, 21.6% for liver, 10.0% for heart, and 4.0% for lung). The overall cancer risk was elevated with 10 656 cases and an incidence of 1375 per 100 000 person-years (SIR, 2.10 95% CI, 2.06-2.14; EAR, 719.3 95% CI, 693.3-745.6 per 100 000 person-years). Risk was increased for 32 different malignancies, some related to known infections (eg, anal cancer, Kaposi sarcoma) and others unrelated (eg, melanoma, thyroid and lip cancers). The most common malignancies with elevated risk were non-Hodgkin lymphoma (n = 1504; incidence: 194.0 per 100 000 person-years; SIR, 7.54 95% CI, 7.17-7.93; EAR, 168.3 95% CI, 158.6-178.4 per 100 000 person-years) and cancers of the lung (n = 1344; incidence: 173.4 per 100 000 person-years; SIR, 1.97 95% CI, 1.86-2.08; EAR, 85.3 95% CI, 76.2-94.8 per 100 000 person-years), liver (n = 930; incidence: 120.0 per 100 000 person-years; SIR, 11.56 95% CI, 10.83-12.33; EAR, 109.6 95% CI, 102.0-117.6 per 100 000 person-years), and kidney (n = 752; incidence: 97.0 per 100 000 person-years; SIR, 4.65 95% CI, 4.32-4.99; EAR, 76.1 95% CI, 69.3-83.3 per 100 000 person-years). Lung cancer risk was most elevated in lung recipients (SIR, 6.13 95% CI, 5.18-7.21) but also increased among other recipients (kidney: SIR, 1.46 95% CI, 1.34-1.59; liver: SIR, 1.95 95% CI, 1.74-2.19; and heart: SIR, 2.67 95% CI, 2.40-2.95). Liver cancer risk was elevated only among liver recipients (SIR, 43.83 95% CI, 40.90-46.91), who manifested exceptional risk in the first 6 months (SIR, 508.97 95% CI, 474.16-545.66) and a 2-fold excess risk for 10 to 15 years thereafter (SIR, 2.22 95% CI, 1.57-3.04). Among kidney recipients, kidney cancer risk was elevated (SIR, 6.66 95% CI, 6.12-7.23) and bimodal in onset time. Kidney cancer risk also was increased in liver recipients (SIR, 1.80 95% CI, 1.40-2.29) and heart recipients (SIR, 2.90 95% CI, 2.32-3.59). CONCLUSION Compared with the general population, recipients of a kidney, liver, heart, or lung transplant have an increased risk for diverse infection-related and unrelated cancers.
Rapid urbanization has resulted in the production of large quantities of sewage sludge over worldwide. The disposal of sewage sludge in landfills or open areas is not considered as an environmentally ...friendly solution. Therefore, the use of sewage sludge as ash can provide a better solution in geotechnical applications. Keeping this in view, this study investigates the possibility of using sewage sludge ash (SSA) to improve the geotechnical properties of soft clay soil. The sewage sludge ash (SSA) is attained by burning the sludge for 2 h at 900°C. The tests conducted on the clayey soil are specific gravity, standard Proctor test, sieve analysis, Atterberg limits, CBR test and unconfined compression test. Three curing times were used for the untreated soils and soils treated with sewage sludge ash (SSA) which are 3 days, 14 days and 28 days. The sewage sludge ash (SSA) was mixed with the soil at different percentages by weight of the soil at 10%, 15% and 20% of dry soil weight. The results showed that the sludge ash increases the unconfined compressive strength of the soft soil at 3, 14, 28-day curing time. The bearing capacity of the soil decreases after adding SSA at the first, but curing time led to improve the bearing capacity of clayey soil after adding SSA and the percentage of SSA of 10% gave the optimum value of CBR. The results indicated that a better performance of the unconfined shear strength was obtained at 28-day curing time. The findings of this research indicate that sewage sludge ash (SSA) can be used to improve the soft clayey soil and further reduce the environmental risks associated with this substantial.
Implications: Rapid urbanization has resulted in the production of large quantities of sewage sludge over worldwide. The disposal of sewage sludge in landfills or open areas is not considered as an environmentally friendly solution. Therefore, the use of sewage sludge as ash can provide a better solution in geotechnical applications. Keeping this in view, this study investigates the possibility of using sewage sludge ash (SSA) to improve geotechnical properties of soft clay soil. In this paper, Sewage Sludge Ash (SSA) is recycled as a new type of chemical addition to soft clay soil and used as a stabilizer to improve a clayey soil. This study proposes that a percent of the total amount of clay to be reinforced is replaced with sewage sludge ash (sewage sludge ash plus soil) in order to achieve the need of improvement in the geotechnical properties of clayey soil and make use of industrial wastes. The sewage sludge ash (SSA) is attained by burning the sludge for 2 h at 900°C. The tests conducted on the clayey soil are specific gravity, standard Proctor test, sieve analysis, Atterberg limits, CBR test, and unconfined compression test. Three curing times were used for the untreated soils and soils treated with sewage sludge ash (SSA) which are 3 days, 14 days, and 28 days. The sludge ash (SSA) was mixed with the soil at different percentages by weight of the soil at 10%, 15%, and 20% of dry soil weight. The results showed that the sludge ash increases the unconfined compression strength of the soft soil at 3, 14, 28-day curing time. The bearing capacity of the soil decreases after adding SSA at the first, but curing time led to improve the bearing capacity of clayey soil after adding SSA and the percentage of SSA of 10% gave the optimum value of CBR. The results indicated that a better performance of the unconfined shear strength was obtained at 28-day curing time. The findings of this research indicate that sewage sludge ash (SSA) can be used to improve the soft clayey soil and further reduce the environmental risks associated with this substantial.
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