The new WHO reference standard allows for the definition of serum antibodies against various SARS-CoV-2 antigens in terms of binding antibody units (BAU/mL) and thus to compare the results of ...different ELISA systems. In this study, the concentration of antibodies (ABs) against both the S- and the N-protein of SARS-CoV-2 as well as serum neutralization activity were evaluated in three patients after a mild course of COVID-19. Serum samples were collected frequently during a period of over one year. Furthermore, in two individuals, the effects of an additional vaccination with a mRNA vaccine containing the S1-RBD sequence on these antibodies were examined. After natural infection, the antibodies (IgA, IgG) against the S1-protein remained elevated above the established cut-off to positivity (S-IgA 60 BAU/mL and S-IgG 50 BAU/mL, respectively) for over a year in all patients, while this was not the case for ABs against the N-protein (cut-off N-IgG 40 BAU/mL, N-IgA 256 BAU/mL). Sera from all patients retained the ability to neutralize SARS-CoV-2 for more than a year. Vaccination resulted in a rapid boost of antibodies to S1-protein but, as expected, not to the N-protein. Most likely, the wide use of the WHO reference preparation will be very useful in determining the individual immune status of patients after an infection with SARS-CoV-2 or after vaccination.
•Marine and non-marine sediments were deposited under humid subtropical and tropical conditions.•The tuffitic sediment gave an Ar/Ar age of 22.2 ± 1.9 Ma, which is the oldest age of tuffitic ...sediments in the NCB so far.•The oldest marine Lower Miocene-Karpatian fauna (NN4) in NCB was determined from the deeper marine deposits.
An integrated stratigraphic study has been performed of the Lower Miocene deposits of the Poljanska and Mala sections (North Croatian Basin), coupled with 40Ar/39Ar dating, geochemical and mineralogical study of tuffitic and silty sand beds. The sedimentary record evidences deposition influenced by volcaniclastic input. The presence of marine, brackish and non–marine microfossils indicates complex palaeoenvironment, palaeoecology, and palaeobiogeography. Four different units were recognized based on their fossil content and sedimentological data. At the base of the Poljanska section (Unit P1), tuffitic sediments bear common endemic brackish, and rare marine to freshwater and terrestrial microfossils. Radiometric 40Ar/39Ar dating obtained on volcanic glass shards from this layer provided an age of 22.2 ± 1.9 Ma correlating with the earliest Early Miocene and indicating the existence of older volcanic activity in the NCB pro tempore. The succession transitions into the brackish-water lacustrine environments (Unit P2). This unit has two presumed different climatic events: a maximum temperature event in the lower part of the interval and one interruption with lower temperatures recognized in the upper part of the interval. The co-occurrence of limnic and marine species in the uppermost part of the investigated sediments (Unit P3) indicates a marine inflow into the coastal lake. It marks the beginning of a salina-type lake formation in Poljanska. In the Mala section (Unit M1), biostratigraphically dated to the NN4 Zone, a transgressive sequence is documented through the presence of marine calcareous nannoplankton, planktonic and benthic foraminifera, and ostracods.
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