Density functional theory calculations of 2D materials and biological molecules have been used to evaluate disease progression through biosensing. In this case, a glycine molecule in normal and ...zwitterionic form was evaluated on its interaction with zigzag single-walled carbon nanotubes, graphene sheets, and molybdenum disulfide sheets. Glycine was rotated in order to interact with the materials at different active sites. Binding and cohesion energies, band gaps, and charge transfer for the systems were obtained. Binding and cohesion for the interaction between normal glycine and 2D materials result in better outcomes with the presence of a dangling bond using van der Waals correction, giving the more stable results for glycine and carbon nanotubes in the plane ZY and glycine with graphene in the plane YX, respectively. For zwitterion glycine, binding and cohesion energies are better without a dangling bond supported on graphene in the plane ZX. Charge transfer results for normal glycine show a better interaction for glycine and molybdenum disulfide in the plane ZY, while for zwitterion glycine, higher charge transfer is reported in graphene (ZX). Furthermore, the density of states of normal glycine exhibits an improvement in the band gap for carbon related materials (more semiconductor behavior) and a slight decrease in semiconductor behavior for molybdenum disulfide.
The physicochemical properties of the cellular environment impact on the biochemical and molecular functions of plant cells.Osmotic stress conditions cause severe changes in intracellular ...macromolecular crowding that plants must adapt to for survival.Recent work has demonstrated that plant cells have mechanisms to sense changes in macromolecular crowding, including plasma membrane and intracellular osmosensors.Tools to dynamically track changes in macromolecular crowding will further contribute to the identification of plant osmosensors.
Osmotic stress conditions occur at multiple stages of plant life. Changes in water availability caused by osmotic stress induce alterations in the mechanical properties of the plasma membrane, its interaction with the cell wall, and the concentration of macromolecules in the cytoplasm. We summarize the reported players involved in the sensing mechanisms of osmotic stress in plants. We discuss how changes in macromolecular crowding are perceived intracellularly by intrinsically disordered regions (IDRs) in proteins. Finally, we review methods for dynamically monitoring macromolecular crowding in living cells and discuss why their implementation is required for the discovery of new plant osmosensors. Elucidating the osmosensing mechanisms will be essential for designing strategies to improve plant productivity in the face of climate change.
Osmotic stress conditions occur at multiple stages of plant life. Changes in water availability caused by osmotic stress induce alterations in the mechanical properties of the plasma membrane, its interaction with the cell wall, and the concentration of macromolecules in the cytoplasm. We summarize the reported players involved in the sensing mechanisms of osmotic stress in plants. We discuss how changes in macromolecular crowding are perceived intracellularly by intrinsically disordered regions (IDRs) in proteins. Finally, we review methods for dynamically monitoring macromolecular crowding in living cells and discuss why their implementation is required for the discovery of new plant osmosensors. Elucidating the osmosensing mechanisms will be essential for designing strategies to improve plant productivity in the face of climate change.
Acute myocardial infarction (AMI) as the main cause of death among cardiovascular diseases is defined as a deficiency of oxygen that generates irreversible tissue necrosis in the heart muscle. For ...diagnostic measurements, the evaluation of cardiac markers concentration like cardiac triponin I (cTnI) in plasma or saliva thought the use of biosensors has become one of the most commonly applied strategies for prognosis of AMI. Inside this diagnostic devices, electrochemical (ECL) ones have been highly encourage to improve sensing capabilities by using different materials and configurations. In this review, the authors presents a summary of studies that involves cTnI detection using ECL biosensors modified with nanomaterials and related mechanisms.
In response to escalating land demands, land reclamation plays a vital role, but this comes with the challenge of ground deformations that threaten livelihoods and safety. To address the growing ...concern, Persistent Scatterer Interferometric Synthetic Aperture Radar (PS-InSAR) is utilized. This remote sensing method is known for its cost-effectiveness, efficiency, and high-resolution capabilities, to monitor vertical ground deformations in Manila Bay Freeport Zone's reclaimed areas. Through adaptation and implementation of PS-InSAR via SARPROZ, measurements along the satellite’s line-of-sight (LOS) is integrated both for ascending and descending geometries using the Nearest Neighbor Vector (NNV) approach to decompose and derive precise vertical displacement values. Correlation analysis with annual leveling data from six benchmarks reveals a strong relationship with correlation coefficient > 0.80. The results indicate relative stability with localized subsidence due to differential settlement and nearby construction. The results from the PS-InSAR technique show that ground deformation on reclaimed lands is expected to continue. Hence, monitoring should be implemented to observe sustained settlements after reclamation, identify areas with significant deformations, detect building and structural deformations, and initiate solutions to reduce the risk of environmental hazards.
In the last decade, the computer vision field has seen significant progress in multimodal data fusion and learning, where multiple sensors, including depth, infrared, and visual, are used to capture ...the environment across diverse spectral ranges. Despite these advancements, there has been no systematic and comprehensive evaluation of fusing RGB-D and thermal modalities to date. While autonomous driving using LiDAR, radar, RGB, and other sensors has garnered substantial research interest, along with the fusion of RGB and depth modalities, the integration of thermal cameras and, specifically, the fusion of RGB-D and thermal data, has received comparatively less attention. This might be partly due to the limited number of publicly available datasets for such applications. This paper provides a comprehensive review of both, state-of-the-art and traditional methods used in fusing RGB-D and thermal camera data for various applications, such as site inspection, human tracking, fault detection, and others. The reviewed literature has been categorised into technical areas, such as 3D reconstruction, segmentation, object detection, available datasets, and other related topics. Following a brief introduction and an overview of the methodology, the study delves into calibration and registration techniques, then examines thermal visualisation and 3D reconstruction, before discussing the application of classic feature-based techniques and modern deep learning approaches. The paper concludes with a discourse on current limitations and potential future research directions. It is hoped that this survey will serve as a valuable reference for researchers looking to familiarise themselves with the latest advancements and contribute to the RGB-DT research field.
REAL-TIME WATER LEVEL MONITORING USING LOW-COST GNSS RECEIVER Dizon, R. D.; Peñales, J. J.; Reyes, R. B. ...
International archives of the photogrammetry, remote sensing and spatial information sciences.,
04/2024, Letnik:
XLVIII-4/W8-2023
Journal Article, Conference Proceeding
Recenzirano
Odprti dostop
Developing an accurate water level monitoring system is one of the measures to mitigate the effects of water-related hazards such as river flooding. While current monitoring systems in the country ...are efficient in terms of accurate and immediate data delivery, these systems can be costly. This study assesses the Global Navigation Satellite System (GNSS) performance of low-cost receiver systems for water level monitoring using real-time kinematic (RTK) solution. A total of 10 days’ valid observation were analyzed to compare the two base-rover receiver setups: 1) low-cost base to low-cost rover (LC-LC) and 2) survey-grade base to low-cost rover (SG-LC) grounded on accuracy, integrity, continuity, availability, and cost. Accuracy results show LC-LC=5.81 cm and SG-LC=5.37 cm mean difference of RTK from in-situ readings. In terms of RTK and post-processing kinematic (PPK) difference for integrity criterion, the RTK SG-LC setup has a lower range of RMS of 0.86 to 1.94 cm versus LC-LC setup of 1.19 to 2.28 cm. For the continuity criterion, the average fixed solutions percentage for the LC-LC setup: RTK=91.43%, PPK=92.92%, whereas for the SG-LC: RTK=95.51%, PPK=98.39%. On availability, the number of valid satellites (NSat) and position dilution of precision (PDOP) of RTK and PPK solutions for each setup are LC-LC: RTK=11, PPK=23, PDOP=1.0 and SG-LC: RTK=11, PPK=24, PDOP=1.9. Lastly, in terms of costing, LC-LC costs Php 58,340 while SG-LC costs Php 1,279,645. Overall, the parity of LC-LC with SG-LC in terms of the five criteria suggests viability of using LC-LC for accurate real-time water level monitoring.
Our objective was to evaluate alterations in sperm chromatin structure in men occupationally exposed to a mixture of organophosphorus pesticides (OP) because these alterations have been proposed to ...compromise male fertility and offspring development. Chromatin susceptibility to in situ acid-induced denaturation structure was assessed by the sperm chromatin structure assay (SCSA). Urinary levels of alkylphosphates (DAP) were used to assess exposure. Diethylthiophosphate (DETP) was the most frequent OP metabolite found in urine samples indicating that compounds derived from thiophosphoric acid were mainly used. Chromatin structure was altered in most samples. About 75% of semen samples were classified as having poor fertility potential (>30% of Percentage of DNA Fragmentation Index DFI%), whereas individuals without OP occupational exposure showed average DFI% values of 9.9%. Most parameters of conventional semen analysis were within normality except for the presence of immature cells (IGC) in which 82% of the samples were above reference values. There were significant direct associations between urinary DETP concentrations and mean DFI and SD-DFI but marginally (
P = 0.079) with DFI%, after adjustment for potential confounders, including IGC. This suggests that OP exposure alters sperm chromatin condensation, which could be reflected in an increased number of cells with greater susceptibility to DNA denaturation. This study showed that human sperm chromatin is a sensitive target to OP exposure and may contribute to adverse reproductive outcomes. Further studies on the relevance of protein phosphorylation as a possible mechanism by which OP alter sperm chromatin are required.
Cross talk between salicylic acid (SA) and jasmonic acid (JA) signaling pathways plays an important role in the regulation and fine tuning of induced defenses that are activated upon pathogen or ...insect attack. Pharmacological experiments revealed that transcription of JA-responsive marker genes, such as PDF1.2 and VSP2, is highly sensitive to suppression by SA. This antagonistic effect of SA on JA signaling was also observed when the JA pathway was biologically activated by necrotrophic pathogens or insect herbivores, and when the SA pathway was triggered by a biotrophic pathogen. Furthermore, all 18 Arabidopsis (Arabidopsis thaliana) accessions tested displayed SA-mediated suppression of JA-responsive gene expression, highlighting the potential significance of this phenomenon in induced plant defenses in nature. During plant-attacker interactions, the kinetics of SA and JA signaling are highly dynamic. Mimicking this dynamic response by applying SA and methyl jasmonate (MeJA) at different concentrations and time intervals revealed that PDF1.2 transcription is readily suppressed when the SA response was activated at or after the onset of the JA response, and that this SA-JA antagonism is long lasting. However, when SA was applied more than 30 h prior to the onset of the JA response, the suppressive effect of SA was completely absent. The window of opportunity of SA to suppress MeJA-induced PDF1.2 transcription coincided with a transient increase in glutathione levels. The glutathione biosynthesis inhibitor L-buthionine-sulfoximine strongly reduced PDF1.2 suppression by SA, suggesting that SA-mediated redox modulation plays an important role in the SA-mediated attenuation of the JA signaling pathway.
PDF
