The three-dimensional swimming tracks of motile microorganisms can be used to identify their species, which holds promise for the rapid identification of bacterial pathogens. The tracks also provide ...detailed information on the cells' responses to external stimuli such as chemical gradients and physical objects. Digital holographic microscopy (DHM) is a well-established, but computationally intensive method for obtaining three-dimensional cell tracks from video microscopy data. We demonstrate that a common neural network (NN) accelerates the analysis of holographic data by an order of magnitude, enabling its use on single-board computers and in real time. We establish a heuristic relationship between the distance of a cell from the focal plane and the size of the bounding box assigned to it by the NN, allowing us to rapidly localise cells in three dimensions as they swim. This technique opens the possibility of providing real-time feedback in experiments, for example by monitoring and adapting the supply of nutrients to a microbial bioreactor in response to changes in the swimming phenotype of microbes, or for rapid identification of bacterial pathogens in drinking water or clinical samples.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Vaccine-inducing immune thrombocytopenia, thrombosis, and bleeding emerge as infrequent and potential complications with mortality risk in healthy subjects. However, differences between survivors and ...non-survivors with SARS-CoV-2 vaccine-induced thrombotic thrombocytopenia (VITT) are unclear.
According to the PRISMA statement, we conducted a systematic review and meta-analysis, and the protocol was registered in PROSPERO. The main objective is to identify differences among survivors and non-survivors of SARS-CoV-2 VITT patients. We systematically searched through PubMed, Scopus, and Web of Science. We included cohorts, case series, and case reports. We classified bleeding complications according to the ISTH definition. Statistics: unpaired Student’s t-test or one-way ANOVA, Wilcoxon, and Kruskal-Wallis.
We systematically searched from January 2021 to June 2021 and identified 51 studies that included 191 patients. Non-survivors had the most severe thrombocytopenia (p 0.02) and lower fibrinogen measurements (p 0.01). Subjects vaccinated with mRNA vaccines (BNT162b2 and mRNA-1273) had an earlier onset of adverse events following immunization (p 0.001). We identified a higher trend of overall thrombotic events (p 0.001) in recipients of viral mechanism-dependent vaccines (Table 2). Non-survivors with cerebral venous sinus thrombosis (CVST) had more severe thrombocytopenia (p 0.01) than survivors with CVST. Finally, 61 % of survivors and 50 % with thrombosis received heparin.
We identified more severe thrombocytopenia, lower fibrinogen measurements, and a higher trend of overall thrombotic events, including CVST and thrombotic storm, particularly with viral mechanisms-dependent vaccines in non-survivors VITT patients.
Herein, we characterize the Toll-like receptor (TLR)-to-NF-κB innate immune pathway of Orbicella faveolata (Of), which is an ecologically important, disease-susceptible, reef-building coral. As ...compared to human TLRs, the intracellular TIR domain of Of-TLR is most similar to TLR4, and it can interact in vitro with the human TLR4 adapter MYD88. Treatment of O. faveolata tissue with lipopolysaccharide, a ligand for mammalian TLR4, resulted in gene expression changes consistent with NF-κB pathway mobilization. Biochemical and cell-based assays revealed that Of-NF-κB resembles the mammalian non-canonical NF-κB protein p100 in that C-terminal truncation results in translocation of Of-NF-κB to the nucleus and increases its DNA-binding and transcriptional activation activities. Moreover, human IκB kinase (IKK) and Of-IKK can both phosphorylate conserved residues in Of-NF-κB in vitro and induce C-terminal processing of Of-NF-κB in vivo. These results are the first characterization of TLR-to-NF-κB signaling proteins in an endangered coral, and suggest that these corals have conserved innate immune pathways.
•A Toll-like receptor to NF-κB pathway is present in the coral Orbicella faveolata.•Activities of O. faveolata TLR and NF-κB proteins are characterized.•LPS treatment of coral tissue activated an NF-κB gene expression profile.
STEMI and NSTEMI: Real-world Study in Mexico (RENASCA) Borrayo-Sánchez, Gabriela; Rosas-Peralta, Martín; Ramírez-Arias, Erick ...
Archives of medical research,
November 2018, 2018-Nov, 2018-11-00, 20181101, Letnik:
49, Številka:
8
Journal Article
Recenzirano
Mexico is the country with the highest mortality due to acute myocardial infarction in adults older than 45 years old according to the OECD (28 vs. 7.5% of the average). The first real-world study, ...RENASCA IMSS, showed a high-risk population at 65%, but 50% without reperfusion strategies. The aim was to describe the clinical presentation, treatment, and outcomes of acute coronary syndromes at the IMSS.
RENASCA IMSS is a nation-wide, prospective, longitudinal-cohort study. We include consecutive patients with an Acute Coronary Syndrome diagnosis (ACC/AHA/ESC) admitted in 177 representative hospitals of the IMSS (166 of second level and 11 of third level of attention). In an electronic database clinical, paraclinical, times, reperfusion treatment, complications, and other variables were assessed. Confidentiality was maintained in data and informed consent was obtained. Registrer calibration was performed with more than 80% of the variables and 80% of the cases.
From March 1, 2014 to December 25, 2017; 21,827 patients were enrolled presenting an average age 63.2 ± 11.7, 75% men (16,259) and 25% women (5,568). The most frequent risk factors were: hypertension (60.5%), smoking (46.8%), diabetes (45.5%), dyslipidemia (35.3%) and metabolic syndrome (39.1%). STEMI diagnosis was established in 73.2% of the patients and NSTEMI in 26.8%. The STEMI group within the Code Infarction showed an improvement in the reperfusion therapy (34.9% before vs. 71.4% after, p ≤0.0001) and reduction of mortality (21.1 vs. 9.4%, p ≤0.0001); while the NSTEMI group showed high risk set by a GRACE score of 131.5 ± 43.7 vs. 135.9 + 41.7, p ≤0.0001. Mortality was more frequent within the STEMI group (14.9 vs. 7.6%, p ≤0.0001).
RENASCA IMSS study represents the largest Acute Coronary Syndromes real-world study in Mexico, demonstrating that the Mexican population has a high risk. Patients with a STEMI diagnosis were more frequently enrolled and were associated with higher mortality and complications; however, there is improvement in the reperfusion therapy and in mortality with the Code Infarction strategy.
Herein, we characterize the Toll-like receptor (TLR)-to-NF-κB innate immune pathway of Orbicella faveolata (Of), which is an ecologically important, disease-susceptible, reef-building coral. As ...compared to human TLRs, the intracellular TIR domain of Of-TLR is most similar to TLR4, and it can interact in vitro with the human TLR4 adapter MYD88. Treatment of O. faveolata tissue with lipopolysaccharide, a ligand for mammalian TLR4, resulted in gene expression changes consistent with NF-κB pathway mobilization. Biochemical and cell-based assays revealed that Of-NF-κB resembles the mammalian non-canonical NF-κB protein p100 in that C-terminal truncation results in translocation of Of-NF-κB to the nucleus and increases its DNA-binding and transcriptional activation activities. Moreover, human IκB kinase (IKK) and Of-IKK can both phosphorylate conserved residues in Of-NF-κB in vitro and induce C-terminal processing of Of-NF-κB in vivo. These results are the first characterization of TLR-to-NF-κB signaling proteins in an endangered coral, and suggest that these corals have conserved innate immune pathways.
► Large sample of sylvatic populations of
Triatoma infestans. ► Analyzed by ITS-2 and mtCytB sequencing. ► Non-Andean origin of the species cannot be discarded. ► Multiple events of
T. infestans ...domestication in Andean and non-Andean areas are suggested.
Triatoma infestans is the main and most widespread vector of Chagas disease in South America. For the first time, a large sample of sylvatic populations of
T. infestans was analyzed by ITS-2 and mtCytB sequencing. ITS-2 showed a low level of polymorphism but revealed a dichotomy between the Andean and non-Andean sylvatic populations. On the contrary, mtCytB sequences showed a high polymorphism (19 haplotypes determined by 35 variable sites) revealing a strong structuring between most of the sylvatic populations and possible ancient isolation and bottleneck in the Northern Andes. The dichotomy Andean vs. non-Andean populations was not observed with this marker. Moreover, mtCytB haplotype genealogies showed that the non-Andean haplotypes would have derived from the Andean ones, supporting somewhat an Andean origin of the species. Nevertheless, a non-Andean origin could not be discarded because a remarkable genetic diversity was found in the non-Andean sample. The comparison of the sylvatic haplotypes with the domestic ones from GenBank suggested multiple events of
T. infestans domestication in Andean and non-Andean areas, instead of a major and unique domestication event in the Bolivian Andes, as previously proposed.
La investigación tuvo como propósito determinar la incidencia del tipo de dieta sobre la sobrevivencia y la ecdisis en la jaiba roja (Callinectes bocourti) en cultivo, para obtener jaiba suave como ...alternativa de producción pesquera en el Caribe colombiano. Se realizaron 2 experimentos: 1. pescado fresco / pescado fresco + Ca+ y 2. pescado fresco / alimento concentrado para camarón (34% proteína). En 4 módulos de recirculación independiente, de 4 acuarios cada uno articulados a filtros biológicos, se sembró un ejemplar/acuario (64 jaibas por ensayo) y se realizaron 3 series de 45 días cada uno. En el experimento 1, sobrevivieron 378 ejemplares, 203 (53.7%) correspondieron a la dieta de pescado fresco y 175 (46.3%) al grupo alimentado con pescado fresco + Ca+. En el experimento 2, sobrevivieron 293 ejemplares (21.8%), 180 (61.4%) consumieron pescado fresco y 113 (38.6%) alimento para camarón. En el experimento 1, no se encuentra relación entre la dieta y la ecdisis. En el experimento 2, se encuentra relación entre el sexo (hembra) y la ecdisis. Se concluye que C. bocourti es una especie con dificultades para manejo en cultivo y la sobrevivencia no depende de la dieta. El sexo (hembra) es una variable por considerar en la ecdisis de C. Bocourti, dado que las hembras requieren mayor energía para el proceso de maduración. La adición de Ca+ al alimento natural no tiene mayor implicación en la ecdisis; puede haber mayor relación con variables ambientales y procesos hormonales que con la ingesta de Ca+.
The purpose of the paper was to determine the incidence of the type of diet on survival and ecdysis in cultured blunt-tooth swimming crab (Callinectes bocourti) to obtain soft crab as alternative ...fish production in the Colombian Caribbean. Two experiments were conducted: 1. fresh fish / fresh fish + Ca+ and 2. fresh fish / shrimp concentrated feed (34% protein). In 4 independent recirculation modules, each with 4 aquariums articulated to biological filters, a specimen/aquarium was planted (64 crabs per test), and 3 series were conducted of 45 days each. In experiment 1, a total of 378 specimens survived; 203 (53.7%) had the fresh fish diet, while 175 (46.3%) were fed fresh fish + Ca+. In experiment 2, a total of 293 (21.8%) specimens survived; 180 (61.4%) consumed fresh fish and 113 (38.6%) shrimp feed. There is no relationship between diet and ecdysis in experiment 1, while there is relationship between sex (female) and ecdysis in experiment 2. It is concluded that C. bocourti is a species with crop management difficulties, and survival does not depend on diet. Sex (female) is a variable to consider in the ecdysis of C. bocourti since females require more energy for the maturation process. Adding Ca+ to natural food has no greater implications in ecdysis. There may be a stronger relationship with environmental variables and hormonal processes than with the Ca+ intake.
O objetivo da pesquisa foi determinar a incidência do tipo de dieta na sobrevivência e ecdise em Callinectes bocourti em cultivo, para obter o siri azul como uma alternativa de produção pesqueira no Caribe colombiano. Foram realizados dois experimentos: 1. peixe fresco / peixe fresco + Ca + e 2. peixe fresco / ração concentrada para camarão (34% de proteína). Em 4 módulos de recirculação independentes, de 4 aquários articulados a filtros biológicos, foi plantado um exemplar / aquário (64 siris por ensaio) e foram realizadas 3 séries de 45 dias cada. No experimento 1378 espécimes sobreviveram, 203 (53.7%) corresponderam à dieta de peixe fresco e 175 (46.3%) corresponderam ao grupo alimentado com peixe fresco + Ca +. No experimento 2293 espécimes (21.8%) sobreviveram, 180 (61.4%) consumiram peixe fresco e 113 (38.6%) ração de camarão. No experimento 1, não há relação entre a dieta e a ecdise. No experimento 2, há uma relação entre o sexo (fêmea) e a ecdise. Conclui-se que C. bocourti é uma espécie com dificuldades para o manejo em cultivo e a sobrevivência não depende da dieta. O sexo (fêmea) é uma variável a ser considerada na ecdise de C. Bocourti, uma vez que as fêmeas necessitam de mais energia para o processo de maturação. A adição de Ca + ao alimento natural não tem implicações importantes na ecdise; pode haver uma maior relação com variáveis ambientais e processos hormonais do que com a ingestão de Ca +.
La investigación tuvo como propósito determinar la incidencia del tipo de dieta sobre la sobrevivencia y la ecdisis en la jaiba roja (Callinectes bocourti) en cultivo, para obtener jaiba suave como alternativa de producción pesquera en el Caribe colombiano. Se realizaron 2 experimentos: 1. pescado fresco / pescado fresco + Ca+ y 2. pescado fresco / alimento concentrado para camarón (34% proteína). En 4 módulos de recirculación independiente, de 4 acuarios cada uno articulados a filtros biológicos, se sembró un ejemplar/acuario (64 jaibas por ensayo) y se realizaron 3 series de 45 días cada uno. En el experimento 1, sobrevivieron 378 ejemplares, 203 (53.7%) correspondieron a la dieta de pescado fresco y 175 (46.3%) al grupo alimentado con pescado fresco + Ca+. En el experimento 2, sobrevivieron 293 ejemplares (21.8%), 180 (61.4%) consumieron pescado fresco y 113 (38.6%) alimento para camarón. En el experimento 1, no se encuentra relación entre la dieta y la ecdisis. En el experimento 2, se encuentra relación entre el sexo (hembra) y la ecdisis. Se concluye que C. bocourti es una especie con dificultades para manejo en cultivo y la sobrevivencia no depende de la dieta. El sexo (hembra) es una variable por considerar en la ecdisis de C. Bocourti, dado que las hembras requieren mayor energía para el proceso de maduración. La adición de Ca+ al alimento natural no tiene mayor implicación en la ecdisis; puede haber mayor relación con variables ambientales y procesos hormonales que con la ingesta de Ca+.
Chagas disease constitutes a relatively prevalent condition in Latin America and is increasing worldwide. With a wide spectrum of clinical subsets. Imaging modalities are critical for adequate ...diagnosis, staging and prognosis of this entity. Currently Echocardiography and Cardiac Magnetic Resonance are the most valuable techniques for this purpose. Evidence for both modalities has increased in the last years, as the role of advanced techniques such as Speckle Tracking Echocardiography has been explored we aim to review the evidence of advanced imaging in the spectrum of patients with Chagas Heart Disease.
PDF
