In winemaking, non-
yeast species contribute important organoleptic complexity. Current interest focuses on abundant and dominant strains characteristically present in the early phase of spontaneous ...alcoholic fermentations. Non-
species are particularly relevant in Port wine production such that the fermentation is prematurely stopped, after the metabolism of only one half of the available sugar, through fortification with aguardente. This work aimed to isolate, identify and characterize non-
species present in spontaneously fermenting Port. To accomplish these goals, yeasts were isolated from a selection of frozen must samples (2012-2016 harvests), using a pre-screening process choosing only the best candidates based on the organoleptic quality of the corresponding fortified wine. From five hundred non-
isolates, twelve species were identified. The three most abundant species,
,
, and
, representing 89% of the isolates, exhibited particularly high diversity with high growth performance variability when exposed to typical stress conditions associated with common enological parameters. Less abundant species included
,
,
,
,
,
,
,
, and
. This is the first study providing insights into the identification and characterization of non-
species responsible for spontaneous Port wine production.
One of the major challenges of neonatal intensive care is the early detection and management of circulatory failure. Routine clinical assessment of the hemodynamic status of newborn infants is ...subjective and inaccurate, emphasizing the need for objective monitoring tools. An overview will be provided about the use of neonatologist-performed echocardiography (NPE) to assess cardiovascular compromise and guide hemodynamic management. Different techniques of central blood flow measurement, such as left and right ventricular output, superior vena cava flow, and descending aortic flow are reviewed focusing on methodology, validation, and available reference values. Recommendations are provided for individualized hemodynamic management guided by NPE.
Advances in neonatal cardiac imaging permit a more comprehensive assessment of myocardial performance in neonates that could not be previously obtained with conventional imaging. Myocardial ...deformation analysis is an emerging quantitative echocardiographic technique to characterize global and regional ventricular function in neonates. Cardiac strain is a measure of tissue deformation and strain rate is the rate at which deformation occurs. These measurements are obtained in neonates using tissue Doppler imaging (TDI) or two-dimensional speckle tracking echocardiography (STE). There is an expanding body of literature describing longitudinal reference ranges and maturational patterns of strain values in term and preterm infants. A thorough understanding of deformation principles, the technical aspects, and clinical applicability is a prerequisite for its routine clinical use in neonates. This review explains the fundamental concepts of deformation imaging in the term and preterm population, describes in a comparative manner the two major deformation imaging methods, provides a practical guide to the acquisition and interpretation of data, and discusses their recognized and developing clinical applications in neonates.
Bacillus Calmette-Guérin (BCG) vaccination has off-target protective effects against infections unrelated to tuberculosis. Among these, murine and human studies suggest that BCG vaccination may ...protect against malaria. We investigated whether BCG vaccination influences neonatal in vitro cytokine responses to Plasmodium falciparum. Blood samples were collected from 108 participants in the Melbourne Infant Study BCG for Allergy and Infection Reduction (MIS BAIR) randomised controlled trial (Clinical trials registration NCT01906853, registered July 2013), seven days after randomisation to neonatal BCG (n = 66) or no BCG vaccination (BCG-naïve, n = 42). In vitro cytokine responses were measured following stimulation with P. falciparum-infected erythrocytes (PfIE) or E. coli.
No difference in the measured cytokines were observed between BCG-vaccinated and BCG-naïve neonates following stimulation with PfIE or E. coli. However, age at which blood was sampled was independently associated with altered cytokine responses to PfIE. Being male was also independently associated with increased TNF-a responses to both PfIE and E. coli.
These findings do not support a role for BCG vaccination in influencing in vitro neonatal cytokine responses to P. falciparum. Older neonates are more likely to develop P. falciparum-induced IFN-γ and IFN-γ-inducible chemokine responses implicated in early protection against malaria and malaria pathogenesis.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Neonatologists can use echocardiography for real-time assessment of the hemodynamic state of neonates to support clinical decision-making. There is a large body of evidence showing the shortcomings ...of conventional echocardiographic indices in neonates. Newer imaging modalities have evolved. Tissue Doppler imaging is a new technique that can provide measurements of myocardial movement and timing of myocardial events and may overcome some of the shortcomings of conventional techniques. The high time resolution and its ability to assess left and right cardiac function make tissue Doppler a favorable technique for assessing heart function in neonates. The aim of this review is to provide an up-to-date overview of tissue Doppler techniques for the assessment of cardiac function in the neonatal context, with focus on measurements from the atrioventricular (AV) plane. We discuss basic concepts, protocol for assessment, feasibility, and limitations, and we report reference values and give examples of its use in neonates.
Cardiac ultrasound techniques are increasingly used in the neonatal intensive care unit to guide cardiorespiratory care of the sick newborn. This is the first in a series of eight review articles ...discussing the current status of "neonatologist-performed echocardiography" (NPE). The aim of this introductory review is to discuss four key elements of NPE. Indications for scanning are summarized to give the neonatologist with echocardiography skills a clear scope of practice. The fundamental physics of ultrasound are explained to allow for image optimization and avoid erroneous conclusions from artifacts. To ensure patient safety during echocardiography recommendations are given to prevent cardiorespiratory instability, hypothermia, infection, and skin lesions. A structured approach to echocardiography, with the same standard views acquired in the same sequence at each scan, is suggested in order to ensure that the neonatologist confirms normal structural anatomy or acquires the necessary images for a pediatric cardiologist to do so when reviewing the scan.
Neonatal heart failure (HF) is a progressive disease caused by cardiovascular and non-cardiovascular abnormalities. The most common cause of neonatal HF is structural congenital heart disease, while ...neonatal cardiomyopathy represents the most common cause of HF in infants with a structurally normal heart. Neonatal cardiomyopathy is a group of diseases manifesting with various morphological and functional phenotypes that affect the heart muscle and alter cardiac performance at, or soon after birth. The clinical presentation of neonates with cardiomyopathy is varied, as are the possible causes of the condition and the severity of disease presentation. Echocardiography is the selected method of choice for diagnostic evaluation, follow-up and analysis of treatment results for cardiomyopathies in neonates. Advances in neonatal echocardiography now permit a more comprehensive assessment of cardiac performance that could not be previously achieved with conventional imaging. In this review, we discuss the current and emerging echocardiographic techniques that aid in the correct diagnostic and pathophysiological assessment of some of the most common etiologies of HF that occur in neonates with a structurally normal heart and acquired cardiomyopathy and we provide recommendations for using these techniques to optimize the management of neonate with HF.
To conduct a pilot randomized trial of an intervention to improve adolescent question-asking and provider education during paediatric diabetes visits.
Adolescents aged 11 to 17 with type 1 diabetes ...and their parents were enrolled from two urban tertiary paediatric clinics. Adolescents were randomised to the intervention group or control group. Adolescent consultations were audio-recorded, their HbA1c level was recorded, and they completed surveys after three clinic appointments. The intervention group completed a question prompt list and watched a video on a tablet with their parents before meeting their doctor and completed a short evaluation after each visit.
Six consultant endocrinologists and ninety-nine adolescents and their parents participated. The intervention increased adolescents’ question asking and provider education in diabetes encounters. Total patient question-asking across the 3 consultations and a higher baseline HbA1c at time one was significantly associated with HbA1c at time three.
Question prompt lists and an educational video are useful tools to increase adolescents’ question-asking and communication between adolescents and their providers.
Interventions that encourage adolescents’ question-asking in healthcare encounters may lead to more meaningful providers-adolescents’ communication and tailored education. Interventions to improve professionals’ listening, communication and educational skills are also required.
•Positive or negative attitudes and behaviours developed during adolescence influence health behaviours into adulthood.•A co-designed question prompt list and video intervention statistically improved adolescent question-asking and provider education.•Promoting adolescent–provider communication may contribute to positive diabetes self-management behaviours.
The CMSSM and NUHM1 after LHC Run 1 Buchmueller, O.; Cavanaugh, R.; Roeck, A. De ...
The European physical journal. C, Particles and fields,
06/2014, Letnik:
74, Številka:
6
Journal Article
Recenzirano
Odprti dostop
We analyze the impact of data from the full Run 1 of the LHC at 7 and 8 TeV on the CMSSM with
μ
>
0
and
<
0
and the NUHM1 with
μ
>
0
, incorporating the constraints imposed by other experiments such ...as precision electroweak measurements, flavour measurements, the cosmological density of cold dark matter and the direct search for the scattering of dark matter particles in the LUX experiment. We use the following results from the LHC experiments: ATLAS searches for events with
E
/
T
accompanied by jets with the full 7 and 8 TeV data, the ATLAS and CMS measurements of the mass of the Higgs boson, the CMS searches for heavy neutral Higgs bosons and a combination of the LHCb and CMS measurements of
BR
(
B
s
→
μ
+
μ
-
)
and
BR
(
B
d
→
μ
+
μ
-
)
. Our results are based on samplings of the parameter spaces of the CMSSM for both
μ
>
0
and
μ
<
0
and of the NUHM1 for
μ
>
0
with 6.8
×
10
6
, 6.2
×
10
6
and 1.6
×
10
7
points, respectively, obtained using the MultiNest tool. The impact of the Higgs-mass constraint is assessed using FeynHiggs 2.10.0, which provides an improved prediction for the masses of the MSSM Higgs bosons in the region of heavy squark masses. It yields in general larger values of
M
h
than previous versions of FeynHiggs, reducing the pressure on the CMSSM and NUHM1. We find that the global
χ
2
functions for the supersymmetric models vary slowly over most of the parameter spaces allowed by the Higgs-mass and the
E
/
T
searches, with best-fit values that are comparable to the
χ
2
/
dof
for the best Standard Model fit. We provide 95 % CL lower limits on the masses of various sparticles and assess the prospects for observing them during Run 2 of the LHC.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
We make a frequentist analysis of the parameter space of the CMSSM and NUHM1, using a Markov Chain Monte Carlo (MCMC) with 95 (221) million points to sample the CMSSM (NUHM1) parameter spaces. Our ...analysis includes the ATLAS search for supersymmetric jets + signals using ∼5/fb of LHC data at 7 TeV, which we apply using PYTHIA and a Delphes implementation that we validate in the relevant parameter regions of the CMSSM and NUHM1. Our analysis also includes the constraint imposed by searches for BR(Bs→μ+μ−) by LHCb, CMS, ATLAS and CDF, and the limit on spin-independent dark matter scattering from 225 live days of XENON100 data. We assume Mh∼125 GeV, and use a full set of electroweak precision and other flavour-physics observables, as well as the cold dark matter density constraint. The ATLAS5/fb constraint has relatively limited effects on the 68 and 95 % CL regions in the (m0,m1/2) planes of the CMSSM and NUHM1. The new BR(Bs→μ+μ−) constraint has greater impacts on these CL regions, and also impacts significantly the 68 and 95 % CL regions in the (MA,tanβ) planes of both models, reducing the best-fit values of tanβ. The recent XENON100 data eliminate the focus-point region in the CMSSM and affect the 68 and 95 % CL regions in the NUHM1. In combination, these new constraints reduce the best-fit values of m0,m1/2 in the CMSSM, and increase the global χ2 from 31.0 to 32.8, reducing the p-value from 12 % to 8.5 %. In the case of the NUHM1, they have little effect on the best-fit values of m0,m1/2, but increase the global χ2 from 28.9 to 31.3, thereby reducing the p-value from 15 % to 9.1 %.
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