Campylorrhinus lateralis, also known as “wry nose,” is a congenital malformation that mainly affects Thoroughbreds. These horses have a unilateral deviation of the maxillae that may be to one side or ...the other side, and it causes airway obstruction and dental malocclusion. The choice of treatment is not necessarily operation; however, the treatment of choice, which aims to repair the maxillae deviation, is surgical to improve the horse’s respiratory condition and correct the dental occlusion. There are currently no reports describing the first surgical technique for such deformity described by Valdez et al. The present study describes the case of a 2-month-old foal of the Mangalarga Marchador breed that was diagnosed with wry nose. The foal presented with accentuated maxillae deviation to the right side, malocclusion of the incisor teeth, and respiratory noises. After diagnosis and physical examination, the foal was treated by surgical correction of the deviation. To correct the deviation, the bone distraction technique with unilateral osteotomy of the right maxillae and fixation of the external bone distractor was chosen. After 90 days, the bone distractor was removed; consequently, malocclusion of the incisors was greatly improved, and respiratory noises were eliminated.
•This is the first case report describing the use of linear bone distractor for correcting congenital lateral deviation (wry nose) in a foal.•The surgical technique with the use of a linear bone distractor to correct congenital lateral deviation in a foal can be used by other veterinary professionals to correct this disease.•The linear bone distractor is a low-cost technological tool that gradually corrects the congenital nasal deviation, promoting the improvement of the animal’s condition and welfare.
Abstract
Background
Multiple technologies are available for detection of circulating tumor cells (CTCs), but standards to evaluate their technical performance are still lacking. This limits the ...applicability of CTC analysis in clinic routine. Therefore, in the context of the CANCER-ID consortium, we established a platform to assess technical validity of CTC detection methods in a European multi-center setting using non-small cell lung cancer (NSCLC) as a model.
Methods
We characterized multiple NSCLC cell lines to define cellular models distinct in their phenotype and molecular characteristics. Standardized tumor-cell-bearing blood samples were prepared at a central laboratory and sent to multiple European laboratories for processing according to standard operating procedures. The data were submitted via an online tool and centrally evaluated. Five CTC-enrichment technologies were tested.
Results
We could identify 2 cytokeratin expressing cell lines with distinct levels of EpCAM expression: NCI-H441 (EpCAMhigh, CKpos) and NCI-H1563 (EpCAMlow, CKpos). Both spiked tumor cell lines were detected by all technologies except for the CellSearch system that failed to enrich EpCAMlow NCI-H1563 cells. Mean recovery rates ranged between 49% and 75% for NCI-H411 and 32% and 76% for NCI-H1563 and significant differences were observed between the tested methods.
Conclusions
This multi-national proficiency testing of CTC-enrichment technologies has importance in the establishment of guidelines for clinically applicable (pre)analytical workflows and the definition of minimal performance qualification requirements prior to clinical validation of technologies. It will remain in operation beyond the funding period of CANCER-ID in the context of the European Liquid Biopsy Society (ELBS).
The high velocity oxygen fuel (HVOF) powder spray process represents the state-of-the-art for thermal spray metallic coatings and can result in very dense, tightly adherent coatings with little or no ...oxidation during the application and low residual stresses. In particular this technology is applied in the automotive industry as well as in the textile and paper machinery and can be an interesting alternative to the use of galvanic processes with high environmental impact. Substitution of hard chromium coatings with new HVOF cermet coatings has been studied in this paper. Coatings obtained from conventional and nano-powders with the chemical composition 75Cr
3C
2–25NiCr were applied on AISI 1045 steel used for cylinders in earth moving machines. A special apparatus was used to perform tribo-corrosion tests. The applied load ranged between 5 and 80N using a rotation speed of the sample of about 200
rpm. The counterface was an alumina block working in a block on ring configuration. Electrochemical methods were used to modify and control the degradation mechanisms and then tribo-corrosion tests were carried out under different working conditions: (a) free corrosion (no applied polarisation); (b) only lubricated wear (by applying a cathodic polarisation); (c) forced corrosion (by applying an anodic current). The behaviour of the cermet coatings was compared to that of conventional hard chromium coatings. It was very interesting to observe that nano-powder coatings displayed a markedly smaller weight loss value with respect to hard chromium and conventional HVOF coatings under all the selected working conditions. This better behaviour can be related to the lower surface roughness and to the better distribution of carbides in the metal matrix and also to the lower porosity of the coating.
In order to promote widespread adoption of appropriate clinical practice, the Italian Society of Hematology (SIE), and the affiliate societies SIES (Italian Society of Experimental Hematology) and ...GITMO (Italian Group for Bone Marrow Transplantation) established to produce guidelines in the most relevant hematological areas. In this article, we report the recommendations for management of T/NK-cell lymphomas, excluding mature T-cell leukaemias.
By using the Grades of Recommendations, Assessment, Development and Evaluation (GRADE) system, we produced evidence-based recommendations for the key clinical questions that needed to be addressed by a critical appraisal of evidence. The consensus methodology was applied to evidence-orphan issues.
Six courses of cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP) or cyclophosphamide, doxorubicin, vincristine, etoposide and prednisone (CHOEP) chemotherapy were recommended for first-line therapy of patients with nodal, intestinal or hepatosplenic T-cell lymphomas (evidence: low; recommendation: do, weak). Except for ALK+ anaplastic large-cell lymphoma and elderly unfit patients, consolidation with high-dose chemotherapy was recommended (evidence: low; recommendation: do, weak). 50 Gy radiotherapy was the recommended first-line therapy for localized extranodal T/NK-cell lymphoma nasal type (evidence: low; recommendation: do, strong), while l-asparaginase-containing chemotherapy regimens were recommended for patients with systemic disease (evidence: very low; recommendation: do, strong).
In adult T/NK-cell lymphomas, GRADE methodology was applicable to a limited number of key therapeutic issues. For the remaining key issues, due to lack of appraisable evidence, recommendations was based on consensus methodology.
Feeding interactions, which determine the dynamics, productivity and stability of an ecosystem to a large extent, constitute fundamental knowledge for understanding the functionality of an ecosystem. ...Within an integrated working context, this study aimed to identify the major prey for the fish species of Araçá Bay through an analysis of stomach contents. Araçá Bay is a sheltered, low tide terrace that presents seasonal hydrodynamics, primary productivity and organism communities. A total of 1253 stomachs from 28 fish species were sampled in March 2013 and January 2014 (austral summer) and in June 2013 (austral winter). A hierarchical cluster analysis (Bray-Curtis similarity index) was performed on the matrix of the index of relative importance (IRI%) of the food items of 15 species collected in both seasons. Five and four major trophic groups were recorded for the summer and winter, respectively (SIMPROF). A two-way PERMANOVA test showed significant differences in the prey compositions among the trophic groups but not the seasons. The annual dendrogram showed four trophic groups: shrimp feeders (8 species), teleostean fish feeders (2 species), copepod feeders (2 species), and benthic invertebrate feeders (3 species). Seasonal species shifts among the trophic groups related to the seasonal recruitment of relatively abundant fish species during the summer were recorded mainly between the teleostean fish feeder and shrimp feeder groups. Overall, the seasonal dynamics of the waters and the consequent prey abundance influenced the resource availability. Resource partitioning and feeding plasticity allowed the species to optimize the use of available prey in the bay. These results represent a contribution toward understanding the local food web, to construct a trophic model, and to contribute to its sustainable integrated management.
•Seasonal shifts in fish species between trophic groups are shown.•No seasonal variations in ingested food are shown.•The results contribute toward constructing a trophic model.
The FAMU experiment aims to accurately measure the hyperfine splitting of the ground state of the muonic hydrogen atom. A measurement of the transfer rate of muons from hydrogen to heavier gases is ...necessary for this purpose. In June 2014, within a preliminary experiment, a pressurized gas-target was exposed to the pulsed low-energy muon beam at the RIKEN RAL muon facility (Rutherford Appleton Laboratory, U.K.). The main goal of the test was the characterization of both the noise induced by the pulsed beam and the X-ray detectors. The apparatus, to some extent rudimental, has served admirably to this task. Technical results have been published that prove the validity of the choices made and pave the way for the next steps. This paper presents the results of physical relevance of measurements of the muon transfer rate to carbon dioxide, oxygen, and argon from non-thermalized excited μp atoms. The analysis methodology and the approach to the systematics errors are useful for the subsequent study of the transfer rate as function of the kinetic energy of the μp currently under way.
The UA9 setup for the double-crystal experiment in CERN-SPS Scandale, W.; Cerutti, F.; Esposito, L.S. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
09/2020, Letnik:
975
Journal Article
Recenzirano
Experiments to measure the electric and the magnetic moments of short-lived baryons using an internal target and two bent crystals in the vicinity of one of the existing LHC detectors were recently ...proposed, in the frame of the Physics Beyond Colliders Working Group at CERN. Investigating fixed-target physics in the LHC with in-vacuum solid targets is an unprecedented challenge. As a preparatory step, the layout of the UA9 experiment, installed in the CERN SPS to explore beam manipulations assisted by bent crystals, has been modified to study the feasibility of the double-crystal scenario in a circular accelerator. Ideally, the first crystal should capture halo protons in channeling states directing them onto the internal target to produce rare baryons, whilst the second crystal, located just downstream of the target, should channel the baryons, rotate their polarization vector and deflect them towards the detector area. The upgraded UA9 layout is presented. Preliminary measurements providing an insight of the beam behavior are reported.
The aim was to evaluate the effects of diet supplementation with red seaweed
Pyropia columbina
or β-carotene on the intestinal microbiota of juvenile Pacú (
Piaractus mesopotamicus)
. Three hundred ...and fifteen fish (body weight 12.4 ± 2.8 g) were stocked in nine 300-L tanks with 35 fishes per tank and fed for 62 days with a basal feed (BG) or the same feed added with
P. columbina
(35 g kg
−1
) (AG) or supplemented with β-carotene (225 g kg
−1
) (β-CG). From fecal samples of three fishes slaughtered per group at 20th, 41th, and 62th days, plate counts of bacterial populations (enterobacteria, lactic acid bacteria—LAB, total aerobes, and psychrotrophs) and DGGE were made. Enterobacteria counts were lower in AG-group than the other groups throughout the experience (
p
= 0.016). LAB/enterobacteria ratio was greater in fishes supplemented with AG on 20th day, showing a greater amount of beneficial bacteria than non-beneficial ones. DGGE profiles resulting from the microbiota of β-CG at 62th day were clustered and separated from the rest of the profiles with a difference of 91%. Margalef richness (
p
= 0.049) and Shannon diversity index (
p
= 0.044) were lower in β-CG than in AG at 62th day. Taxonomic characterization showed the presence of three phylum: Bacteroidetes, Firmicutes, and Fusobacteria. This is the first study in which the microbiota of juvenile Pacú fed with biofunctional diets was evaluated. Future interventions that modify the bacterial ecosystem could improve the productive performance and ensure the safety of the pacu meat intended for consumption.
The genus Xanthomonas is a diverse and economically important group of bacterial phytopathogens, belonging to the γ-subdivision of the Proteobacteria. Xanthomonas axonopodis pv. citri (Xac) causes ...citrus canker, which affects most commercial citrus cultivars, resulting in significant losses worldwide. Symptoms include canker lesions, leading to abscission of fruit and leaves and general tree decline. Xanthomonas campestris pv. campestris (Xcc) causes black rot, which affects crucifers such as Brassica and Arabidopsis. Symptoms include marginal leaf chlorosis and darkening of vascular tissue, accompanied by extensive wilting and necrosis. Xanthomonas campestris pv. campestris is grown commercially to produce the exopolysaccharide xanthan gum, which is used as a viscosifying and stabilizing agent in many industries. Here we report and compare the complete genome sequences of Xac and Xcc. Their distinct disease phenotypes and host ranges belie a high degree of similarity at the genomic level. More than 80% of genes are shared, and gene order is conserved along most of their respective chromosomes. We identified several groups of strain-specific genes, and on the basis of these groups we propose mechanisms that may explain the differing host specificities and pathogenic processes.
Celotno besedilo
Dostopno za:
DOBA, IJS, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Aim
In Italy, the ISPED CARD initiative was launched to measure and improve quality of care in children and adolescents with type 1 diabetes.
Methods
Process and outcome indicators and the related ...information derived from electronic medical records were identified. A network of pediatric diabetes centers was created on a voluntary basis.
Results
Overall, 20 centers provided data on 3284 patients aged < = 18 years. HbA1c was monitored ≥ 2/year in 81.2% of the cases. BMI was monitored ≥ 1/year in 99.0%, lipid profile in 45.3%, and blood pressure in 91.7%. Pubertal status, albuminuria, eye examination, and screening of celiac disease and thyroiditis were underreported. From 2017 to 2021, average HbA1c levels decreased from 7.8 ± 1.2 to 7.6 ± 1.3%, while patients with LDL cholesterol > 100 mg/dl increased from 18.9 to 36.7%. Prevalence of patients with elevated blood pressure and BMI/SDS values also increased. In 2021, 44.7% of patients were treated with the newest basal insulins, while use of regular human insulin had dropped to 7.7%. Use of insulin pump remained stable (37.9%).
Conclusions
This report documents the feasibility of the ISPED CARD initiative and shows lights and shadows in the care provided. Improving care, increasing number of centers, and ameliorating data recording represent future challenges.
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