The current study aimed to describe the fabrication of a composite patch by incorporating marine algae powders (MAPs) into poly-lactic acid (PLA) for bone tissue engineering. The prepared composite ...patch was functionalized with the co-polymer, poly (2-hydroxyethyl methacrylate-co-ethylene glycol dimethacrylate) (p(HEMA-co-EGDMA)) via initiated chemical vapor deposition (iCVD) to improve its wettability and overall biocompatibility. The iCVD functionalized MAP-PLA composite patch showed superior cell interaction of human osteoblasts. Following the surface functionalization by p(HEMA-co-EGDMA) via the iCVD technique, a highly hydrophilic patch was achieved without tailoring any morphological and structural properties. Moreover, the iCVD modified composite patch exhibited ideal cell adhesion for human osteoblasts, thus making the proposed patch suitable for potential biomedical applications including bone tissue engineering, especially in the fields of dentistry and orthopedy.
The reconstruction of bony defects in the alveolar crest poses challenges in dental practice. Guided tissue regeneration (GTR) and guided bone regeneration (GBR) procedures utilize barriers to ...promote bone regeneration and prevent epithelial growth. This study focuses on evaluating the antibacterial properties of marine algae-polylactic acid (PLA) composite membranes compared to commercially available collagen membranes. Marine algae (Corallina elongata, Galaxaura oblongata, Cystoseira compressa, Saragassum vulgare, and Stypopodium schimperi) were processed into powders and blended with PLA to fabricate composite membranes. Cytocompatibility assays using human periodontal ligament fibroblasts (
n
= 3) were performed to evaluate biocompatibility. Antibacterial effects were assessed through colony-forming units (CFU) and scanning electron microscopy (SEM) analysis of bacterial colonization on the membranes. The cytocompatibility assays demonstrated suitable biocompatibility of all marine algae-PLA composite membranes with human periodontal ligament fibroblasts. Antibacterial assessment revealed that Sargassum vulgare-PLA membranes exhibited the highest resistance to bacterial colonization, followed by Galaxaura oblongata-PLA and Cystoseira compressa-PLA membranes. SEM analysis confirmed these findings and revealed smooth surface textures for the marine algae-PLA membranes compared to the fibrous and porous structures of collagen membranes. Marine algae-PLA composite membranes show promising antibacterial properties and cytocompatibility for guided bone and tissue regeneration applications. Sargassum vulgare-PLA membranes demonstrated the highest resistance against bacterial colonization. These findings suggest that marine algae-PLA composite membranes could serve as effective biomaterials for infection control and tissue regeneration. Further in vivo validation and investigation of biodegradation properties are necessary to explore their clinical potential.
Graphical Abstract
Biodegradable collagen-based materials have been preferred as scaffolds and grafts for diverse clinical applications in density and orthopedy. Besides the advantages of using such bio-originated ...materials, the use of collagen matrices increases the risk of infection transmission through the cells or the tissues of the graft/scaffold. In addition, such collagen-based solutions are not counted as economically feasible approaches due to their high production cost. In recent years, incorporation of marine algae in synthetic polymers has been considered as an alternative method for preparation grafts/scaffolds since they represent abundant and cheap source of potential biopolymers. Current work aims to propose a novel composite patch prepared by blending Sargassum vulgare powders (SVP) to polylactide (PLA) as an alternative to the porcine-derived membranes. SVP-PLA composite patches were produced by using a modified solvent casting method. Following detailed material characterization to assess the cytocompatibility, human osteoblasts (HOBs) and osteosarcoma cells (SaOS-2) were seeded on neat PLA and SVP-PLA patches. MTT and BrdU assays indicated a greater cytocompatibility and higher proliferation for HOBs cultured on SVP-PLA composite than for those cultured on neat PLA. SaOS-2 cells cultured on SVP-PLA exhibited a significant decrease in cell proliferation. The composite patch described herein exhibits an antiproliferative effect against SaOS-2 cells without impairing HOBs' adhesion and proliferation.
In the present study, collagen was obtained from tissues of the invasive sea urchin (Diadema setosum) rapidly spread in the Mediterranean Sea. As a result, the yield of collagen isolated from sea ...urchin was determined to be 23.78±1.33% (dry weight). As a result of SDS-Page analysis, it was determined that it contains (α1)2α2, (Molecular Weight (MA) 124, 114 kDa) and ß chains (MA 245 kDa) similar to calfskin collagen. In the amino acid analysis of collagen, it was determined that the imino acid (proline+hydroxyproline) content was 196.1 residues/1000 residues. The functional bands of amide A (3301 cm-1), B (2924 cm-1), I (1643 cm-1), II (1550 cm-1), and III (1242 cm-1) functional bands were visualized in the FTIR spectrum. These results were like other collagen sources. Invasive sea urchin was used as a source of collagen for the first time in the present study. An alternative source of collagen to mammalian collagen, which is used commercially in many industries such as biomedicine, food and cosmetics, was isolated for the first time from D. setosum. It was proposed that marine collagen can be used as an alternative source of collagen and a functional component in areas including food, cosmetics, and pharmaceutical industries.
Bu çalışmada, Akdeniz'de hızla yayılan istilacı denizkestanesi (Diadema setosum) dokularından kolajen elde edilmiştir. Sonuç olarak, denizkestanesinden elde edilen kolajenin verimi %23.78±1.33 (kuru ağırlık) olarak belirlenmiştir. SDS-Page analizi sonucunda, dana derisi kolajenine benzeyen (α1)2α2, (Molekül Ağırlığı (MA) 124, 114 kDa) ve ß zincirleri (MA 245 kDa) içerdiği tespit edilmiştir. Kolajenin amino asit analizinde imino asit (prolin+hidroksiprolin) içeriğinin 196.1 kalıntı/1000 kalıntı olduğu belirlenmiştir. FTIR spektrumuna göre Amid A (3301 cm-1), Amide B (2924 cm-1), Amide I (1643 cm-1), Amide II (1550 cm-1) ve Amide III (1242 cm-1) fonksiyonel bantları görüntülenmiştir. İstilacı denizkestanesinden kolajen ilk defa bu çalışma ile elde edilmiştir. Biyotıp, gıda ve kozmetik gibi birçok endüstride ticari olarak kullanılan memeli kolajenine alternatif olarak istilacı denizkestanesinden (D. setosum) kolajen ilk kez elde edilmiştir. Elde edilen bu kolajeninin gıda, kozmetik ve ilaç endüstrileri gibi alanlarda alternatif bir kolajen kaynağı ve fonksiyonel bir bileşen olarak kullanılabileceği önerilmiştir.
The calcium-alginate/multi-walled carbon nanotube hybrid beads (Ca-ALG/MWCNT-COOH) as a novel kind of matrix were fabricated and characterized in detailed.
l
-Asparaginase (
l
-ASNase), which is ...important chemotherapeutic enzyme-drug in leukemia, was immobilized on the Ca-ALG/MWCNT-COOH hybrid beads. To the best of our knowledge, this is the first study using Ca-ALG/MWCNT-COOH hybrid beads for
l
-ASNase immobilization. Our characterization investigations displayed that the hybridization between ALG and MWCNT-COOH caused significant changes on the surface morphology and structure. ALG of 0.5% (w/v), CaCl
2
of 0.2 M concentration, enzyme of 187.5 U and bead size of 2 mm was found to be best with respect to enzyme loading efficiency. The enzyme was loaded a high yield (97.0%) on these hybrid beads. Remarkably, the tolerance of immobilized enzyme developed towards temperature and pH changes. The maximum activity for the free enzyme was observed at 35 °C, pH 7.5, whereas the immobilized enzyme showed maximum activity at 45 °C pH 8.5. After immobilization, storage stability of enzyme improved and retained more than 70% of its initial activity after 4 weeks at ~ 30 °C as compared with free enzyme which showed only 20% of residual activity. After immobilization, Km value decreased 1.27-fold compared to free counterpart, indicating increased the affinity between the substrate and enzyme. Moreover, immobilized enzyme maintained more than 36% of its original activity even after consecutive 14 reuse. As result, it is worthy of noting that this kind of hybrid materials may become a promising support material for the immobilization of commercial enzymes in areas such as industrial and medical.
Graphic Abstract
The aim of this study is to define the biosorption properties of Alginate, AC1 and AC2 beads composed of collagen obtained from sea urchin Diadema setosum and alginate obtained from Sargassum ...vulgare, for Pb (II) from aqueous solutions. Raw alginate beads (A), 2:1 alginate-modified-collagen beads (AC1), and 3:1 alginate-modified collagen beads (AC2) were successfully synthesized, characterized, and removal capacity of Pb (II) in aqueous solutions studied. The biosorption of Pb (II) ions by the synthesized beads was studied and the effect of parameters such as initial concentration, contact time, and solution pH on the biosorption of Pb (II) ions was investigated. Langmuir isotherm model provides satisfactory estimations of homogeneous monolayer biosorption capacity, the highest Pb (II) determined values being 248.76 mg/g for Alginate beads, 344.83 mg/g for AC1 beads, and 303.03 mg/g for AC2 beads. Moreover, AC1 beads were determined to be homogeneous monolayer and multilayer at two different stages. In the former stage, Pb (II) were quickly diffused to the outer surface of absorbent, and in the latter stage, ions were slowly biosorbed to the inner surface. The results obtained in this study showed that Alginate, AC1 and AC2 beads can be used effectively to remove Pb (II) ions from aqueous solutions. Another finding from this study was that the AC1 beads did not disintegration and significantly swelled in size relative to the other beads. Another important finding of this study was that AC1 beads were not deformed and increased in size.
Facioscapulohumeral Muscular Dystrophy (FSHD) is in the top three list of all dystrophieswith an approximate 1:8000 incidence. It is not a life-threatening disease; however, the progressionof the ...disease extends over being wheelchair bound. Despite some drug trials continuing,including DUX4 inhibition, TGF-ß inhibition and resokine which promote healthier muscle, there isnot an applicable treatment option for FSHD today. Still, there is a need for new agents to heal, stopor at least slow down muscle wasting. Current FSHD studies involving nutraceuticals as vitamin C,vitamin E, coenzyme Q10, zinc, selenium, and phytochemicals as curcumin or genistein, daidzeinflavonoids provide promising treatment strategies. In this review, we present the clinical and molecularnature of FSHD and focus on nutraceuticals and phytochemicals that may alleviate FSHD. Inthe light of the association of impaired pathophysiological FSHD pathways with nutraceuticals andphytochemicals according to the literature, we present both studied and novel approaches that cancontribute to FSHD treatment.
Purpose: In our study, it was aimed to investigate the preventive effect of milrinone on renal damage in experimental controlled non-heart-beating donors (NHBDs) model. Materials and Methods: Sixteen ...rats randomly divided into 2 groups, 8 rats in each were used. Group 1 was control, group 2 was milrinone group. Group 1 rats received 1.25 ml 0.09% NaCl intraperitoneally equivalent to the milrinone diluted volume. Group 2 rats were administered intraperitoneally with 0.5 mg/kg of milrinone 2 hours before cardiac arrest. After the cardiac arrest, left nephrectomy was applied to the rats. Malondialdehyde, superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) activities, Caspase-3 (apoptotic index) and histopathological evaluation were performed in the tissues. Results: In the milrinone group, the total injury score was significantly lower relative to the control group (p = 0.001). Caspase-3 staining was moderately strong in the control group but weaker in the milrinone group. Apoptotic index was significantly lower in the milrinone group compared to the control group (p = 0.001). In comparison between groups, SOD and GPx in the milrinone group was significantly higher than the control group (p = 0.008, p = 0.006). Conclusions: Milrinone has been shown to be effective in the prevention of tissue damage due to oxidative stress and inflammatory process in the renal of warm ischemia in the experimental NHBDs model and in protecting the renal. Milrinone increases antioxidant activity while reducing apoptosis. Systemic administration of milrinone prior to cardiac arrest may be beneficial. Administration of milrinone to the recipient in the perioperative period may contribute to donor function.
Celotno besedilo
Dostopno za:
DOBA, IJS, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Facioscapulohumeral Muscular Dystrophy (FSHD) is in the top three list of all dystrophies with an approximate 1:8000 incidence. It is not a life-threatening disease; however, the progression of the ...disease extends over being wheelchair bound. Despite some drug trials continuing, including DUX4 inhibition, TGF-ß inhibition and resokine which promote healthier muscle, there is not an applicable treatment option for FSHD today. Still, there is a need for new agents to heal, stop or at least slow down muscle wasting. Current FSHD studies involving nutraceuticals as vitamin C, vitamin E, coenzyme Q10, zinc, selenium, and phytochemicals as curcumin or genistein, daidzein flavonoids provide promising treatment strategies. In this review, we present the clinical and molecular nature of FSHD and focus on nutraceuticals and phytochemicals that may alleviate FSHD. In the light of the association of impaired pathophysiological FSHD pathways with nutraceuticals and phytochemicals according to the literature, we present both studied and novel approaches that can contribute to FSHD treatment.
PDF
