At present EVD epidemic, which claimed the lives of more than 10000 people, is still underway in West Africa Countries. Promptness and quality of laboratory diagnostics, alongside with delivered ...anti-epidemic measures predetermine efficacy of Ebola response operation. Due to a lack of means for the specific prophylaxis and treatment of the disease, asymptomatic patients are discharged from hospitals, based on criteria recommended by WHO, which might be insufficient. Viral RNA is detected in different clinical samples taken from the patients even at the advanced stages of convalescence, which requires essential investigation of peculiarities of Ebola persistence in various biological fluids. The article contains the data on the studies of biological samples, obtained from a female patient diagnosed with Ebola virus disease, applying various methods and techniques.
Nonpathogenic
Exophiala nigrum
(black yeasts) unicelular organisms of the Baikal Lake were used as a model system for determination of small iron concentrations in the samples grown without or with ...controlled amount of maghemite nanoparticles (MNPs) in nutrient. MNPs were produced by the electrophysical laser target evaporation technique. Electrostatically stabilized suspensions were prepared using sodium citrate solutions in distilled water. We assumed that one maximum permissive dose of ionic iron in water 1 MPD is equal to 0.3 mg/L. For biological experiments Saburo liquid nutrient medium was prepared with iron concentrations of 0, 10
2
, 10
3
and 10
4
MPD. One ml of
E. Nigrum
cell suspension was added to Saburo liquid nutrient for 24 hours exposure. Followed by sowing onto a solid agar Saburo for 30 days colonies grows. Biosamples for electron microscopy, magnetic and total reflection X-ray fluorescence spectroscopy measurements were collected simultaneously. We were able to comparatively analyze the trace concentrations of iron in the yeast of the order of 10 ppm for control group and 600 ppm for the group grown in the presence of 10
4
MPD of iron.
In this work was proposed a new radiotherapy enhancement method consisting of the administration of magnetic nanoparticles into the cells with further irradiation with a gamma-ray beam. As a result, ...adjusting the energy distribution of a gamma-ray beam and
57
Fe abundance it is possible to achieve an extremely intensive electron emission because of a nuclear resonance. The produced conversion and Auger electrons can be used as an effective tool for DNA lesions production. We developed a Monte Carlo model for an electron and gamma emission by
57
Fe nucleus using the Geant4 program package. The parameters of a resonant absorption were taken from Mössbauer spectra of magnetite nanoparticles synthesized for the administration into live cells. The space distribution of the radiation dose showed an increase in the dose of 2–2.5 times in the case of the natural abundance and more than 50 times in the case of the 66 % enrichment of the nanoparticles.
A system for taking automatic measurements of the impedance of composite polymer films at frequencies of 10 to 100 kHz and temperatures of up to 250°C is described.
Late Barremian to early Albian palaeotemperatures have been determined through oxygen isotope analyses of well-preserved brachiopod, bivalve, gastropod and ammonite shells, and belemnite rostra from ...the Caucasus. Results from calcitic invertebrate skeletal elements from the upper Barremian Sartousiana, Securiformis and Waagenoides zones indicate palaeotemperatures of 19.2, 12.0–17.1 and 11.3–14.3 °C, respectively. In contrast, early Aptian fossils of the Weissi and Deshayesi zones reveal significantly higher palaeotemperatures (20.5–22.3 and 17.1 °C, respectively), than those obtained from brachiopod shells of the Securiformis Zone. Isotopic palaeotemperature data are not available for the Furcata Zone in the Caucasus. The warmest conditions in our study interval developed during the late Aptian Melchioris-Abichi Chrone according to data on bivalves, gastropods and the ammonite Parahoplites (20.8–27.1 °C) and the Subnodosocostatum Chrone (?25.3 °C), on the basis of a shell of the oyster Amphidonte. However, the potential influence of minor freshwater incursions into the upper epipelagic zone in which Amphidonte lived, as well as the predominance of ammonites that preferred cool conditions in the Subnodosocostatum Zone, leads to an alternative interpretation in which cooling initiated at the beginning of the late Aptian. There remains considerable uncertainty in the interpretation of δ18O values in fossils from the Subnodosocostatum Zone but it is possible that one or two cooling events interrupted the warmest conditions during the late Aptian. The highest δ13C values derived from fossils found in the Subnodosocostatun Zone (up to 8.3 and 3.9‰ for shallow-water bivalves and deeper-dwelling belemnite taxa, respectively) have been linked to an episode of late Aptian volcanic activity. On the basis of our stable oxygen isotope record, two ethological groups can be recognised among Aptian ammonites from the Caucasus: cool and thermophilic dwellers. All Early Cretaceous belemnites from this area seem to have had a preference for cool-water conditions.
•New evidences on Cretaceous belemnites of the Caucasus suggest that all of them were rather cool-water-loving animals.•Representatives of two main ethological groups are recognized among Aptian ammonoids from the Caucasus.•One or two cooling phases could interrupt warmest conditions of the Caucasus in the late Aptian.•Highest δ13C values may have been linked to one of the episodes of late Aptian volcanic activity.
Novel synthesis of 3-iodo-ortho-carborane Safronov, Alexander V.; Hawthorne, M. Frederick
Inorganica Chimica Acta,
09/2011, Letnik:
375, Številka:
1
Journal Article
Recenzirano
A new and convenient route to 3-iodo-ortho-carborane was developed starting from thallium ortho-dicarbollide which readily reacts with boron triiodide in hexane to give the title compound in 70-80% ...yield and on a scale ranging from several milligrams to tens of grams of 3-iodo-ortho-carborane. Display omitted
► A new synthesis route for 3-iodo-ortho-carborane is described. ► The conventional synthetic procedure leads to the increased by-product formation with increasing reaction scale. ► The reaction of thallium dicarbollide with boron triiodide in hexanes gives high yields of 3-iodo-ortho-carborane. ► No substantial amounts of any by-products are being formed on a scale extending from several milligrams to tens of grams.
A new and convenient route to 3-iodo-ortho-carborane was developed starting from thallium ortho-dicarbollide. This stable dicarbollide derivative can be isolated and purified thus avoiding undesirable by-product formation observed in a more conventional synthetic approach. Thallium dicarbollide readily reacts with boron triiodide in hexane to give the title compound in 70–80% yield and on a scale ranging from several milligrams to tens of grams of 3-iodo-ortho-carborane.
The main requirement for powders for additive 3d technologies is the spherical shape of the particles. Such particles are most compactly packed into a certain volume and ensure the "fluidity" of the ...powder composition in the supply systems of the material with minimal resistance. Proceeding from the peculiarities of the methods for the production of spherical powders with the aim of obtaining spherical granules of regulated granularity, the technology of electroerosive dispersion is proposed, which is characterized by relatively low energy costs and ecological purity of the process. Based on the results of the studies aimed at investigating the mechanical properties of sintered samples from cobalt-chrome powders obtained for additive technologies by electroerosive dispersion, it was established that the average Vickers hardness of sintered samples is 14,63 Gpa; the porosity of the sintered samples is 6,15%.
The beryllium (Be) plasma-facing components (PFCs) of the ITER first wall (FW) were tested in the plasma gun QSPA-Be under pulsed plasma heat loads of 0.5-ms duration relevant to those expected in ...ITER during transient plasma events (edge-localized modes and disruptions). The experiments were performed for different Be grades (Russian TGP-56FW and US S65-C). The measured Be melting threshold decreases from 0.5 MJm
−2
down to 0.4 MJm
−2
with Be initial temperature increasing in the range of 250-500 °C. Under plasma heat loads on the exposed surface below the melting point the Be PFC erosion was mainly due to melting of the plasma-facing and lateral edges of the Be tiles. Under plasma heat loads above the melting point the Be PFC erosion was mainly due to intense melt layer movement and splashing. The Be melt layer behavior at 0.5 and 1.0 MJm
−2
is similar to early investigated W melt layer behavior at higher heat loads of 1.0 and 1.5 MJm
−2
correspondingly. Unlike W the Be erosion rate significantly increases with initial temperature in the range of 250-500 °C. These experimental observations are supported by calculation of temperature dynamics and melt layer thickness dynamics.
Baikal-GVD has recently published its first measurement of the diffuse astrophysical neutrino flux, performed using high-energy cascade-like events. We further explore the Baikal-GVD cascade dataset ...collected in 2018-2022, with the aim to identify possible associations between the Baikal-GVD neutrinos and known astrophysical sources. We leverage the relatively high angular resolution of the Baikal-GVD neutrino telescope (2-3 deg.), made possible by the use of liquid water as the detection medium, enabling the study of astrophysical point sources even with cascade events. We estimate the telescope's sensitivity in the cascade channel for high-energy astrophysical sources and refine our analysis prescriptions using Monte-Carlo simulations. We primarily focus on cascades with energies exceeding 100 TeV, which we employ to search for correlation with radio-bright blazars. Although the currently limited neutrino sample size provides no statistically significant effects, our analysis suggests a number of possible associations with both extragalactic and Galactic sources. Specifically, we present an analysis of an observed triplet of neutrino candidate events in the Galactic plane, focusing on its potential connection with certain Galactic sources, and discuss the coincidence of cascades with several bright and flaring blazars.
Taking into consideration experience in utilization of “AmpliSens EBOV (ZAIRE)-FL” in the setting of SAET mobile laboratory complex, carried out has been the improvement of the panel with a view to ...elapsed time reduction and analytical sensitivity enhancement. It provides for the analytical sensitivity increase up to 200 copies per ml, which is 10 times higher than the previous measurement, that accounted for 2·103 copies per ml. The timing of the assay has been reduced up to 1 h 25 min, which is 25 % less than the time needed for the standard study. Modification of “AmpliSens EBOV (ZAIRE)-FL” panel benefits to the enhancement of epidemiological surveillance activities intended to stop the spread of Zaire ebolavirus in West Africa and prevent its importation into the Russian Federation.
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