Primary aldosteronism is the most common form of secondary hypertension with a prevalence of 5–10% in hypertensive patients. Aldosterone-producing adenoma (APA) is a subtype of primary aldosteronism, ...and somatic mutations in KCNJ5, ATP1A1, ATP2B3, CACNA1D, CLCN2, or CTNNB1 were identified and recognized to drive aldosterone production and/or contribute to tumorigenesis in APA. Mutations of KCNJ5, ATP1A1, ATP2B3, CACNA1D, and CLCN2 are known to activate calcium signaling, and its activation potentiate CYP11B2 (aldosterone synthesis) transcription in adrenal cells. Transcriptome analyses combined with bioinformatics using APA samples were conductive for each gene mutation mediated pivotal pathway, gene ontology, and clustering. Several important intracellular molecules in increase aldosterone production were detected by transcriptome analysis, and additional functional analyses demonstrated intracellular molecular mechanisms of aldosterone production which focused on calcium signal, CYP11B2 transcription and translation. Furthermore, DNA methylation analysis revealed that promoter region of CYP11B2 was entirely hypomethylated, but that of other steroidogenic enzymes were not in APA. Integration of transcriptome and DNA methylome analysis clarified some DNA methylation associated gene expression, and the transcripts have a role for aldosterone production. In this article, we reviewed the intracellular molecular mechanisms of aldosterone production in APA, and discussed future challenges for basic studies leading to clinical practice.
To detect volatile organic compounds (VOCs) in indoor air in small enterprises in La Plata city and surrounding areas, sampling was conducted using passive diffusion monitors (3M-3500) and analysis ...of the samples were performed byCG-FID. Analytic methodology was optimized for 23 VOCs (n-alkanes, cycloalkanes, aromatic and chlorinated compounds, ketones and terpenes compounds) by determining the recovery factor and detection limit for each analyte. Different recovery values were obtained by desorbing with a mixture of dichloromethane: methanol (50:50), with a standard deviation lower than 5%. Enterprise analyzed included chemical analysis laboratories, sewing workrooms, electromechanical repair and car painting centers, take away food shops, and a photocopy center. The highest levels of VOCs were found to be in electromechanical repair and car painting centers (hexane, BTEX, CHCl3, CCl4) followed by chemical analysis laboratories and sewing workrooms. Cancer and noncancer risks were assessed using conventional approaches (HQ and LCR, US EPA) using the benzene, trichloroethylene, chloroform for cancer risk, and toluene, xylene and n-hexane, for noncancer risks as markers. The results showed different LCR for benzene and trichloroethylene between the different indoor environments analyzed (electromechanical repair and car painting center≫others) and chloroform (laboratory>others), but comparing with the results obtained by other research, are in similar order of magnitude for equivalents activities. Similar finding were founded for HQ. Comparing these results with the worker protection legislation the electromechanical repair and car painting center and chemical analysis laboratories are close to the limits advised by OSHA and ACGIH. These facts show the importance of the use of abatement technologies for the complete reduction of VOCs levels, to mitigate their impact in the worker’s health and their venting to the atmosphere.
► Indoor air of small enterprises was sampled whit 3M-3500 monitors. ► Desorption was developed for 23 VOCs representatives of SMEs. ► Cancer and noncancer risks were assessed using conventional approaches (US EPA).
Display omitted
•Al2O3/limestone composites were prepared by ball milling with Al2O3 contents in the range between 5 and 20%wt.•The composites were subjected to calcination-carbonation cycles at ...conditions for CO2 capture and energy storage.•Diverse types of calcium aluminate were formed as depending on the Calcium Looping conditions.•High Al-content leads to deactivation mainly by the formation of Ca3Al2O6 at CO2 capture conditions.•At energy storage conditions, the residual effective conversion of the composites is twice that of limestone.
This work reports the Calcium-Looping (CaL) multicycle performance under energy storage and CO2 capture conditions of different Al-composites prepared by milling mixtures of nanoalumina and natural limestone powders. The micro- and nanostructure of the composites have been analyzed by X-ray diffraction, scanning electron microscopy and high-resolution transmission electron microscopy as affected by the type of CaL conditions employed, either for energy storage in Concentrated Solar Power (CSP) plants or for post-combustion CO2 capture. Two types of calcium aluminates are formed under these diverse CaL conditions. A calcium aluminate with ratio Ca/Al < 1 (Ca4Al6O13) is formed under CaL-CSP conditions, which helps stabilize the CaO microstructure and mitigate pore-plugging. On the other hand, a crystalline phase Ca3Al2O6 is formed (Ca/Al > 1) under CaL-CO2 capture conditions presumably due to the higher calcination temperature, which withdraws from the sorbent a relatively higher amount of active Ca. Moreover, the addition of nano-alumina, and the consequent generation of calcium aluminate, affects in a diverse way the microstructure and morphology of the CaO particles as depending on the CaL application, which critically modifies the performance of the composites.
Forest are highly vulnerable to global change drivers, such as an increase in wildfire events. Learning more about how and why different post‐fire management strategies regulate the ability of forest ...ecosystem properties (e.g. plant diversity and function) to simultaneously recover after wildfire and provide multiple ecosystem functions is of critical importance.
This study aims to evaluate how unburned, burned managed and burned unmanaged plots regulate the responses of multiple forest ecosystem properties (e.g. plant diversity, nutrient cycling, soil carbon stocks, water regulation, decomposition and wood production) and overall multifunctionality to wildfires. In September 2017, we selected two post‐fire management strategies in a 3‐km2 watershed previously affected by a wildfire in July 2012: contour‐felled log debris (CFD), log erosion barriers area (LEB), and also unburned and unmanaged plots (BNA). We randomly distributed 12 plots among the three post‐fire management strategies (three plots per treatment) and unburned.
The results showed that multiple forest ecosystem properties were significantly affected by wildfire and that specific post‐fire management treatment (e.g. LEB and CFD) can be used to efficiently support plant diversity and ecosystem functioning. Our results revealed that the general indicators of ecosystem functions decreased in Mediterranean forests after wildfires and post‐fire management strategies (LEB and CFD) significantly helped to recover the ecosystems’ short‐term community‐level properties and ecosystem functions (5 years after a wildfire event) to pre‐fire levels.
Synthesis and applications. These findings demonstrate that multiple ecosystem functions are affected by wildfires in Mediterranean forests and show that post‐fire management treatments can promote multifunctionality and plant diversity. Our results unfold the potential of log erosion barriers (LEB) and contour‐felled log debris (CFD) as effective strategies for recovering community‐level properties and forest functions in the short term.
These findings demonstrate that multiple ecosystem functions are affected by wildfires in Mediterranean forests and show that post‐fire management treatments can promote multifunctionality and plant diversity. Our results unfold the potential of log erosion barriers (LEB) and contour‐felled log debris (CFD) as effective strategies for recovering community‐level properties and forest functions in the short term.
•Adaptive Neuro-Fuzzy Inference System based robust scheme provide more accuracy•Combine features text, images & frames for phishing detection proof more detection•This is the first work that ...reflects the best unified text, image and frame feature•Using SVM for phishing web classification and relate the use with the current result•The proposed solution achieves 98.3% accuracies
A phishing attack is one of the most significant problems faced by online users because of its enormous effect on the online activities performed. In recent years, phishing attacks continue to escalate in frequency, severity and impact. Several solutions, using various methodologies, have been proposed in the literature to counter the web-phishing threats. Notwithstanding, the existing technology cannot detect the new phishing attacks accurately due to the insufficient integration of features of the text, image and frame in the evaluation process. The use of related features of images, frames and text of legitimate and non-legitimate websites and associated artificial intelligence algorithms to develop an integrated method to address these together. This paper presents an Adaptive Neuro-Fuzzy Inference System (ANFIS) based robust scheme using the integrated features of the text, images and frames for web-phishing detection and protection. The proposed solution achieves 98.3% accuracies. To our best knowledge, this is the first work that considers the best-integrated text, image and frame feature based solution for phishing detection scheme.
Primary aldosteronism (PA) represents the most common cause of secondary hypertension, but little is known regarding its adrenal cellular origins. Recently, aldosterone-producing cell clusters ...(APCCs) with high expression of aldosterone synthase (CYP11B2) were found in both normal and PA adrenal tissue. PA-causing aldosterone-producing adenomas (APAs) harbor mutations in genes encoding ion channels/pumps that alter intracellular calcium homeostasis and cause renin-independent aldosterone production through increased CYP11B2 expression. Herein, we hypothesized that APCCs have APArelated aldosterone-stimulating somatic gene mutations. APCCs were studied in 42 normal adrenals from kidney donors. To clarify APCC molecular characteristics, we used microarrays to compare the APCC transcriptome with conventional adrenocortical zones zona glomerulosa (ZG), zona fasciculata, and zona reticularis. The APCC transcriptome was most similar to ZG but with an enhanced capacity to produce aldosterone. To determine if APCCs harbored APA-related mutations, we performed targeted next generation sequencing of DNA from 23 APCCs and adjacent normal adrenal tissue isolated from both formalin-fixed, paraffin-embedded, and frozen tissues. Known aldosterone driver mutations were identified in 8 of 23 (35%) APCCs, including mutations incalcium channel, voltage-dependent, L-type, α1D-subunit(CACNA1D; 6 of 23 APCCs) andATPase, Na⁺/K⁺ transporting, α1-polypeptide(ATP1A1; 2 of 23 APCCs), which were not observed in the adjacent normal adrenal tissue. Overall, we show three major findings: (i) APCCs are common in normal adrenals, (ii) APCCs harbor somatic mutations known to cause excess aldosterone production, and (iii) the mutation spectrum of aldosterone-driving mutations is different in APCCs from that seen in APA. These results provide molecular support for APCC as a precursor of PA.
Display omitted
•Small sizes of particles are required in order to separate the different fractions.•Inverse flotation process is an efficient green technology to separate fractions.•Superficial air ...velocity is the main variable in the inverse flotation process.•Inverse flotation is a green process because the pulṕs pH is 7.0 during the test.
The generation of electrical and electronic waste is increasing day by day; recycling is attractive because of the metallic fraction containing these. Nevertheless, conventional techniques are highly polluting. The comminution of the printed circuit boards followed by an inverse flotation process is a clean technique that allows one to separate the metallic fraction from the non-metallic fraction. It was found that particle size and superficial air velocity are the main variables in the separation of the different fractions. In this way an efficient separation is achieved by avoiding the environmental contamination coupled with the possible utilization of the different fractions obtained.
CoCrMo alloys are used in a wide range of applications because of their acceptable fatigue strength and corrosion resistance. However, CoCrMo-based components exhibit a deleterious effect of wear. ...For this reason, surface modification through ceramic coatings has become in a useful alternative to improve mechanical resistance. The main aim of the present study was to investigate the tribological behavior of a system of diffusional boride layers formed on the substrate of CoCrMo alloy. For this purpose, different boron diffusion heat treatment parameters were performed: 1223, 1248 and 1273 K by 6, 8 and 10 h, respectively. Surfaces were characterized using scanning electron microscopy (SEM), surface roughness measurement, micro-hardness testing, and X-ray diffraction. The tribological response was studied by means of ball on disc tribometer under dry-sliding conditions tracking their friction coefficient, mass loss, wear rate and surface damage by stereoscopy and SEM. Boride coating at 1223 K by 6 h showed the better wear resistance, increased it 6 times relative to the wear resistance obtained for the CoCr alloy untreated sample.
•CoCr borided sample at 1223 K – 6 h increased 6 times the wear resistance in comparison with the CoCr untreated sample.•The wear resistant borided layer was correlated with temperature and time parameters of boronizing process.•The maximum hardness value achieved on the borided surface was 5 times higher than the hardness of the substrate.•The main wear mechanism was severe abrasion for the untreated sample and surface fatigue for the borided samples.
The genetic origin of novel traits is a central but challenging puzzle in evolutionary biology. Among snakes, phospholipase A2 (PLA2)-related toxins have evolved in different lineages to function as ...potent neurotoxins, myotoxins, or hemotoxins. Here, we traced the genomic origin and evolution of PLA2 toxins by examining PLA2 gene number, organization, and expression in both neurotoxic and non-neurotoxic rattlesnakes. We found that even though most North American rattlesnakes do not produce neurotoxins, the genes of a specialized heterodimeric neurotoxin predate the origin of rattlesnakes and were present in their last common ancestor (∼22 mya). The neurotoxin genes were then deleted independently in the lineages leading to the Western Diamondback (Crotalus atrox) and Eastern Diamondback (C. adamanteus) rattlesnakes (∼6 mya), while a PLA2 myotoxin gene retained in C. atrox was deleted from the neurotoxic Mojave rattlesnake (C. scutulatus; ∼4 mya). The rapid evolution of PLA2 gene number appears to be due to transposon invasion that provided a template for non-allelic homologous recombination.
•Contrary to assumptions, the most recent common rattlesnake ancestor was neurotoxic•Gene number in the phospholipase A2 complex is evolutionarily dynamic•Neurotoxin and myotoxin genes were lost independently in different lineages•Venom diversity is due to both gene duplication and gene loss
Dowell et al. show that a phospholipase A2 gene complex encoding a family of venom toxins expanded early in rattlesnake evolution and that individual neurotoxin or myotoxin genes were independently deleted recently in different species lineages. These losses presumably reflect ecological shifts in prey choice that made certain toxins dispensable.
PDF
