Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The ...aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours.inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.
Abstract
Double networks were prepared from guayule rubber (GR), deproteinized natural rubber (DPNR), and styrene-butadiene rubber (SBR), and their properties compared to conventional “single ...networks” having the same crosslink density. Substantial residual strains (> 150%) were obtained in all double networks, whereby the modulus parallel to the residual strain was enhanced. For the two strain-crystallizing elastomers, the fatigue resistance of the double networks (for extensions parallel to the residual strain) was higher than for their single network counterparts. Moreover, the guayule rubber, which is more strain-crystallizable than DPNR, exhibited the greater enhancement. For the amorphous SBR, on the other hand, the network structure had an insignificant effect on the fatigue life. These results demonstrate that longer mechanical fatigue lifetimes in double network rubbers are a consequence of their intrinsic orientation. This provides the capacity to retain crystallinity at the front of growing cracks, even in the absence of stress. The origin of the improved fatigue resistance is similar to the mechanism responsible for the better performance of strain-crystallizing rubbers subjected to non-relaxing cyclic deformations.
We searched for the μ+μ− decay of a light vector gauge boson, also known as dark photon, in the e+e−→μ+μ−γISR process by means of the Initial State Radiation (ISR) method. We used 1.93 fb−1 of data ...collected by the KLOE experiment at the DAΦNE ϕ-factory. No structures have been observed over the irreducible μ+μ− background. A 90% CL limit on the ratio ε2=α′/α between the dark coupling constant and the fine structure constant of 3×10−6–2×10−7 has been set in the dark photon mass region between 519 MeV and 973 MeV. This new limit has been combined with the published result obtained investigating the hypothesis of the dark photon decaying into hadrons in e+e−→π+π−γISR events. The combined 90% CL limit increases the sensitivity especially in the ρ–ω interference region and excludes ε2 greater than (13−2)×10−7. For dark photon masses greater than 600 MeV the combined limit is lower than 8 ×10−7 resulting more stringent than present constraints from other experiments.
We have measured the running of the effective QED coupling constant α(s) in the time-like region 0.6<s<0.975 GeV with the KLOE detector at DAΦNE using the Initial-State Radiation process e+e−→μ+μ−γ. ...It represents the first measurement of the running of α(s) in this energy region. Our results show a more than 5σ significance of the hadronic contribution to the running of α(s), which is the strongest direct evidence both in time- and space-like regions achieved in a single measurement. By using the e+e−→π+π− cross section measured by KLOE, the real and imaginary parts of the shift Δα(s) have been extracted. From a fit of the real part of Δα(s) and assuming the lepton universality the branching ratio BR(ω→μ+μ−)=(6.6±1.4stat±1.7syst)⋅10−5 has been determined.
Abstract Babesia bovis is the causative agent of babesiosis, a tick-borne disease that is a major cause of loss to livestock production in Latin America. Vaccination against Babesia species ...represents a major challenge against cattle morbidity and mortality in enzootic areas. The aim of this study was to evaluate the capacity of Bacille Calmette-Guerin (BCG) to deliver the rhoptry associated protein (RAP-1) antigen of B. bovis and to stimulate specific cellular and humoral immune responses in mice. Two of five mycobacterial expression vectors efficiently expressed the antigen. These constructs were subsequently studied in vivo following three immunization protocols. The construct with the greatest in vivo stability proved to be the one that induced the strongest immune responses. Our data support the hypothesis that specific T lymphocyte priming by rBCG can be employed as a component of a combined vaccine strategy to induce long-lasting humoral and cellular immune responsiveness towards B. bovis and encourage further work on the application of rBCG to the development of Babesia vaccines.
We present a new limit on the production of a light dark-force mediator with the KLOE detector at DAΦNE. This boson, called U, has been searched for in the decay ϕ→ηU, U→e+e−, analyzing the decay ...η→π0π0π0 in a data sample of 1.7 fb−1. No structures are observed in the e+e− invariant mass distribution over the background. This search is combined with a previous result obtained from the decay η→π+π−π0, increasing the sensitivity. We set an upper limit at 90% C.L. on the ratio between the U boson coupling constant and the fine structure constant of α′/α<1.7×10−5 for 30<MU<400 MeV and α′/α⩽8×10−6 for the sub-region 50<MU<210 MeV. This result assumes the Vector Meson Dominance expectations for the ϕηγ⁎ transition form factor. The dependence of this limit on the transition form factor has also been studied.
Neutral kaon pairs produced in ϕ decays in anti-symmetric entangled state can be exploited to search for violation of CPT symmetry and Lorentz invariance. We present an analysis of the CP-violating ...process ϕ→KSKL→π+π−π+π− based on 1.7 fb−1 of data collected by the KLOE experiment at the Frascati ϕ-factory DAΦNE. The data are used to perform a measurement of the CPT-violating parameters Δaμ for neutral kaons in the context of the Standard Model Extension framework. The parameters measured in the reference frame of the fixed stars are:Δa0=(−6.0±7.7stat±3.1syst)×10−18 GeV,ΔaX=(0.9±1.5stat±0.6syst)×10−18 GeV,ΔaY=(−2.0±1.5stat±0.5syst)×10−18 GeV,ΔaZ=(3.1±1.7stat±0.5syst)×10−18 GeV. These are presently the most precise measurements in the quark sector of the Standard Model Extension.
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