Mesenchymal stem cells have been widely used in the treatment of various chronic diseases. The objective of this survey was to evaluate the therapeutic and regenerative potential of stem cells from ...adipose tissue (ASCs) in the milk production recovery repair of tissue injury in mastitis goats treated with antimicrobial agents prior to cell therapy. After the diagnosis of mastitis and treatment with gentamicin, eight lactating goats were selected for cellular and subsequent therapy, physical-chemical analysis of milk, ultrasonographic and histopathological examinations. The ASCs were taken from the subcutaneous fat of a young goat cultivated in vitro, marked with Qdots-655 and injected in the left mammary gland, being the right mammary gland used as the control. After 30 days the ultrasonographic and histopathological analyzes were repeated and, in the first lactation period, the physical-chemical analysis of the milk was reapeated. Before the cellular therapy, the physical-chemical quality of the milk was compromised and the ultrasonographic and histopathological analysis revealed a chronic inflammatory process and fibrous tissue. The marking of the ASCs with Qdots enabled the tracking, by fluorescence microscopy (BX41-OLYMPUS), in the mammary tissue. In the ASCs therapy, cultures showed high cellularity and characteristics favorable to preclinical studies; with the therapy the physical-chemical parameters of the milk, fat, protein, temperature and pH showed significant differences among the groups; five animals treated with ASCs reconstituted the functionality of the gland and the connective tissue reduced in quantity and inflammatory infiltrate cells. ASCs have potential for the possible regeneration of fibrous mastitis lesions in the mammary gland, however, it would be necessary to increase injection time for the histopathological analysis, since the reconstitution of the glandular acini within the assessed period was not finalized. ASCs can be used to reestablish milk production in goat with chronic mastitis repair mammary lesions, with potential to be a promising clinical alternative for animal rehabilitation for productivity.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The eucalyptus gall wasp, Leptocybe invasa Fischer&LaSalle, causes severe damage to susceptible plants of the genera Eucalyptus L'Her. and Corymbia K.D. Hill&L.A.S. Johnson, in which it induces galls ...in the midribs and petioles of young leaves and in the internodes of branch apices. Even though the insect attacks trees of all ages, the infestations are generally more severe in nursery seedlings and young plantations than in older plantations, and the wasp is more often found in warmer and drier zones than in colder and wetter zones. The pest was first observed infesting plants in a commercial plantation of Eucalyptus clones (VS058) from the crossbreeding of Eucalyptus tereticornis Sm. x Eucalyptus camaldulensis Dehnh., located in the municipality of Peixe, Tocantins. This initial infestation was detected in an area of 180ha, where it spreaded quickly to neighboring plantations, reaching an area of 3000 ha planted with clones from the crossbreeding mentioned above. After detection in the field, the wasp attack was also found in clonal rooted cuttings of Eucalyptus clones from this same crossbreeding.
Objectives
Our aim was to investigate factors associated with baseline blood telomere length in participants enrolled in NEAT 001/ANRS 143, a randomized, open‐label trial comparing ritonavir‐boosted ...darunavir (DRV/r) plus raltegravir (RAL) with DRV/r plus tenofovir disoproxil fumarate/emtricitabine (TDF/FTC) in antiretroviral therapy (ART)‐naïve HIV‐positive adults.
Methods
A cross‐sectional study of 201 randomly selected participants who had stored samples available was carried out. We measured telomere length (i.e. the relative telomere length, calculated as the telomere to single copy gene ratio) at baseline with monochrome quantitative multiplex polymerase chain reaction (PCR). We used multivariable predictive linear regression to calculate mean differences and 95% confidence intervals (CIs) for the association between baseline telomere length and baseline characteristics.
Results
The baseline characteristics of the 201 participants did not differ from those of the 805 participants in the parent trial population: 89% were male, the mean age was 39 years, 83.6% were Caucasian, 93% acquired HIV infection via sexual transmission, the mean estimated time since HIV diagnosis was 2.1 years, the mean HIV‐1 RNA load was 4.7 log10 HIV‐1 RNA copies/mL, the mean nadir and baseline CD4 counts were 301 and 324 cells/μL, respectively, and the mean CD4:CD8 ratio was 0.4. In the univariate analysis, shorter telomere length was associated with older age (per 10 years) (P < 0.001), HIV‐1 RNA ≥ 100 000 copies/mL (P = 0.001), CD4 count < 200 cells/μL (P = 0.037), lower CD4:CD8 ratio (P = 0.018), statin treatment (P = 0.004), and current alcohol consumption (P = 0.035). In the multivariable analysis, older age (P < 0.001) and HIV RNA ≥ 100 000 copies/mL (P = 0.054) were independently associated with shorter telomere length.
Conclusions
Both age and HIV RNA viral load correlated with shorter blood telomere length in untreated persons living with HIV. These results suggest that HIV infection and age have synergistic and independent impacts upon immunosenescence.
Context
The monoclinic L-histidine crystal is critical for protein structure and function and is also found in the myelin of brain nerve cells. This study numerically examines its structural, ...electronic, and optical properties. Our findings indicate that the L-histidine crystal has an insulating band gap of approximately 4.38 eV. Additionally, electron and hole effective masses range between 3.92
m
0
–15.33
m
0
and 4.16
m
0
–7.53
m
0
, respectively. Furthermore, our investigation suggests that the L-histidine crystal is an excellent UV collector due to its strong optical absorption activity for photon energies exceeding 3.5 eV.
Methods
To investigate the structural, electronic, and optical properties of L-histidine crystals, we used the Biovia Materials Studio software to conduct Density Functional Theory (DFT) simulations as implemented in the CASTEP code. Our DFT calculations were performed using the generalized gradient approximation (GGA) as parameterized by the Perdew-Burke-Ernzerhof (PBE) exchange-correlation functional, with an additional dispersion energy correction (PBE
+
TS) based on the model proposed by Tkatchenko and Scheffler to describe van der Waals interactions. Additionally, we employed the norm-conserving pseudopotential to treat core electrons.
The effects of deactivation by coke deposition after the
n
-heptane cracking reaction were evaluated on crystallinity, acidity and activation energies for the coke thermal oxidation process in ...microporous and hierarchical ZSM-23 zeolites. The fresh and coked catalysts were characterized by X-ray diffraction, temperature-programmed desorption of ammonia and thermogravimetric analysis. The Flynn–Wall–Ozawa and Vyazovkin kinetic models were used to determine the activation energy of the catalyst regeneration process, using integral dynamic curves from thermogravimetric analysis data obtained with different heating rates. The generation of micro-/mesoporous structures provided a beneficial effect by inhibiting the loss of crystallinity and preserving a certain degree of acidity after catalyst deactivation. The hierarchical ZSM-23 zeolites showed lower deposited coke amounts and activation energy values for the coke thermal oxidation process, providing greater stability, activity and lower energy level required for regeneration in comparison with the microporous zeolites.
Recent agricultural expansion into regions of the Brazilian savannah or Cerrado has improved agricultural knowledge and management practices. Under this biome’s favorable conditions, the incidence of ...diseases such as gummy stem blight (GSB) could be a limiting factor for some crops (i.e.,
Cucumis melo
L.), due to high losses in productivity and fruit quality. We conducted field experiments to assess the temporal progression of the incidence and severity of GSB disease in melon plants under five pesticide treatments and three growing seasons (dry, rainy, and transition periods). Furthermore, the susceptibility of six cultivars of melon to GSB was evaluated with and without pesticide application. In the dry season, although the incidence of the disease was high and dependent on the applied pesticides, its severity was low, allowing the crop cycle to be completed and marketable fruits to be harvested. However, in the rainy and transition periods, the high incidence and severity of the disease recorded in all plants, independent of the pesticides used, resulted in a premature end to the growth of the melon crops. Moreover, the commonly cultivated cultivar (Eldorado 300) showed a higher incidence and severity of the disease than the other potential cultivars. The results suggest that melon crops should be grown exclusively under the Brazilian Cerrado conditions during the dry season, dominated by favorable climatic conditions for the melon and unfavorable conditions to the pathogen. Other cultivars need to be investigated for their suitability to this region.
Abstract
The X-ARAPUCA device is the baseline choice for the photon
detection system of the first far detector module of the DUNE
experiment. We present the results of the first complete
...characterization of a small scale X-ARAPUCA prototype, which is a
slice of a full DUNE module. Its total detection efficiency in
liquid argon was measured with three different ionizing radiations:
α particles, γ's and muons and resulted to be
∼2.2% when the active silicon photomultipliers were biased at
+5.0 V
of over voltage, corresponding to a Photon Detection
Efficiency around 50% at room temperature. This value comfortably
satisfies the requirements of the first DUNE far detector module
(detection efficiency >2.0%) and allows to achieve an energy
resolution comparable to the one achievable with the Time Projection
Chambers for energies below 10 MeV, which is the region relevant
for Supernova neutrino detection.
The indiscriminate use of pesticides has caused several damages to the environment, in particular the pollution of water reservoirs, so that this has motivated the development of techniques to ...minimize its consequences. One of the main surface water pollutants is glyphosate, which is a widely used herbicide for weed control. Therefore, in this work, computational simulations were used with density functional theory and molecular dynamics to theoretically verify if C
fullerene is capable of adsorbing glyphosate in aqueous media. As a result, we showed through the adsorption energies, molecular dynamics methods, and infrared absorption that C
can adsorb glyphosate molecules in at least three distinct configurations, either in vacuum or in water, which theoretically indicates it as a good candidate for removal of this herbicide from water by nanotechnology techniques.
Summary
Little data are available on renal toxicity exerted by direct‐acting antivirals (DAAs) in real life. The aim of this study was to assess the impact of direct‐acting antivirals against ...hepatitis C virus infection currently used in Spain and Portugal on the estimated glomerular filtration rate (eGFR) in clinical practise. From an international, prospective multicohort study, patients treated with DAAs for at least 12 weeks and with eGFR ≥30 mL/min per 1.73 m2 at baseline were selected. eGFR was determined using the CKD‐EPI formula. A total of 1131 patients were included; 658 (58%) were HIV/HCV‐coinfected patients. Among the 901 patients treated for 12 weeks, median (interquartile range) eGFR was 100 (87‐107) at baseline vs 97 (85‐105) mL/min per 1.73 m2 at week 12 of follow‐up (FU12) post‐treatment (P < .001). For HIV‐coinfected subjects who received tenofovir plus a ritonavir‐boosted HIV protease inhibitor (PI/r), baseline vs FU12 eGFR were 104 (86‐109) vs 104 (91‐110) mL/min per 1.73 m2 (P = .913). Among subjects receiving ombitasvir/paritaprevir with or without dasabuvir, eGFR did not show any significant change. Of 1100 subjects with eGFR >60 mL/min per 1.73 m2 at baseline, 22 (2%) had eGFR <60 mL/min per 1.73 m2 at FU12, but none presented with eGFR <30 mL/min per 1.73 m2. In conclusion, eGFR slightly declines during therapy with all‐oral DAAs and this effect persists up to 12 weeks after stopping treatment in subjects with normal to moderately impaired renal function, regardless of HIV status. Concomitant use of tenofovir plus PI/r does not seem to have an impact on eGFR.
To satisfy the world's constantly increasing demand for energy, a suitable mix of different energy sources has to be devised. In this scenario, an important role could be played by nuclear energy, ...provided that major safety, waste and proliferation issues affecting current nuclear reactors are satisfactorily addressed. To this purpose, a large effort has been under way for a few years towards the development of advanced nuclear systems with the aim of closing the fuel cycle. Generation IV reactors, with full or partial waste recycling capability, accelerator driven systems, as well as new fuel cycles are the main options being investigated. The design of advanced systems requires improvements in basic nuclear data, such as cross-sections for neutron-induced reactions on actinides. In this paper, the main concepts of advanced reactor systems are described, together with the related needs of new and accurate nuclear data. The present activity in this field at the neutron facility n_TOF at CERN is discussed.
Neutron-induced reaction cross-sections of actinides have been obtained at CERN n_TOF to improve the accuracy of basic data needed for the design of advanced nuclear energy systems.
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