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Wear-mediated osteolysis is a common complication occurring around implanted prosthesis, which ultimately leads to bone loss with mechanical instability and the need for surgical ...revision. At the moment, revision surgery is the only effective treatment. The aim of this study was to assess the efficacy of pulsed electromagnetic fields (PEMFs) and platelet rich plasma (PRP), alone and in association, in a clinically relevant in vivo model of periprosthetic osteolysis. Titanium alloy pins were implanted intramedullary in distal femurs of male inbred rats and, after osseointegration, polyethylene particles were injected intra-articularly to induce osteolysis. Animals were divided in four groups of treatment: PEMFs, PRP, PEMFs + PRP and no treatment. Microtomography was performed during the course of experiments to monitor bone stock and microarchitecture. Histology, histomorphometry, immunohistochemistry and biomechanics were evaluated after treatments. Biophysical and biological stimulations significantly enhanced bone to implant contact, bone volume and bone microhardness and reduced fibrous capsule formation and the number of osteoclasts around implants. Among treatments, PEMFs alone and in association with PRP exerted better results than PRP alone. Present data suggest that biophysical stimulation, with or without the enrichment with platelet derived growth factors, might be a safe, mini-invasive and conservative therapy for counteracting osteolysis and prompting bone formation around implants.
Pulsed electromagnetic fields (PEMFs) and platelet rich plasma (PRP) show anabolic and anti-inflammatory effects and they are already been used in clinical practice, but separately. To date, there are no preclinical in vivo studies evaluating their combined efficacy in periprosthetic osteolysis, in bone tissue microarchitecture and in biomechanics. The aim of the present study was to evaluate the effects of PEMFs and PRP in vivo, when administered individually and in combination in the treatment of periprosthetic wear mediated ostelysis, and in restoring the osteogenetic properties of perimplant bone tissue and its biomechanical competence. The combination of PEMFs and PRP could be employed for counteracting the ostelysis process in a conservative and non surgical manner.
Loss of bladder control is common after spinal cord injury (SCI) and no causal therapies are available. Here we investigated whether function-blocking antibodies against the nerve-fiber growth ...inhibitory protein Nogo-A applied to rats with severe SCI could prevent development of neurogenic lower urinary tract dysfunction. Bladder function of rats with SCI was repeatedly assessed by urodynamic examination in fully awake animals. Four weeks after SCI, detrusor sphincter dyssynergia had developed in all untreated or control antibody-infused animals. In contrast, 2 weeks of intrathecal anti-Nogo-A antibody treatment led to significantly reduced aberrant maximum detrusor pressure during voiding and a reduction of the abnormal EMG high-frequency activity in the external urethral sphincter. Anatomically, we found higher densities of fibers originating from the pontine micturition center in the lumbosacral gray matter in the anti-Nogo-A antibody-treated animals, as well as a reduced number of inhibitory interneurons in lamina X. These results suggest that anti-Nogo-A therapy could also have positive effects on bladder function clinically.
After spinal cord injury, loss of bladder control is common. Detrusor sphincter dyssynergia is a potentially life-threatening consequence. Currently, only symptomatic treatment options are available. First causal treatment options are urgently needed in humans. In this work, we show that function-blocking antibodies against the nerve-fiber growth inhibitory protein Nogo-A applied to rats with severe spinal cord injury could prevent development of neurogenic lower urinary tract dysfunction, in particular detrusor sphincter dyssynergia. Anti-Nogo-A therapy has entered phase II clinical trial in humans and might therefore soon be the first causal treatment option for neurogenic lower urinary tract dysfunction.
COVID-19 is still a global challenge in regard for management and therapy. Pulmonary embolism (PE) seems to have a higher prevalence in COVID-19 instead of non-COVID patients. Clinical and laboratory ...parameters related with PE are still unknown.
We conducted a retrospective unicentre study in Alto Vicentino Hospital between March 1st, 2020, and January 31st, 2021 in patients admitted for COVID-19 tested with a RT-PCR nasal swab. Data about patients studied with computed tomography pulmonary angiogram (CTPA) because of PE suspicion were collected, as their clinical and laboratory parameters too.
2621 patients were admitted for COVID-19 in Alto Vicentino Hospital between March 1st, 2020, and January 31st, 2021 and in 267 of them a CTPA was performed finding 50 PE (18.7%). Only non-Caucasian race (OR = 5.44; 95% CI 1.22–24.35; p = 0.027) and previous VTE (OR = 5.3; 95% CI 1.09–26.17; p = 0.039) were found to be independently associated with PE.
PE is a frequent complication of COVID-19 and clinician need high degree of suspicion because clinical and laboratoristic parameters cannot drive diagnosis.
•This study tries to find a correlation between COVID-19 and pulmonary embolism.•COVID-19 infection appears to be most correlated with thromboembolic events.•Unfortunately, clinical and laboratoristic parameters cannot drive diagnosis of pulmonary embolism in COVID-19 patients.
Context. The variety and complexity of long duration gamma-ray burst (LGRB) light curves (LCs) encode a wealth of information about the way LGRB engines release their energy following the collapse of ...the progenitor massive star. Thus far, attempts to characterise GRB LCs have focused on a number of properties, such as the minimum variability timescale and power density spectra (both ensemble average and individual), or considering different definitions of variability. In parallel, a characterisation as a stochastic process has been pursued by studying the distributions of waiting times, peak flux, and fluence of individual peaks that can be identified within GRB time profiles. However, an important question remains as to whether the diversity of GRB profiles can be described in terms of a common stochastic process. Aims. Here, we address this issue by extracting and modelling, for the first time, the distribution of the number of peaks within a GRB profile. Methods. We analysed four different GRB catalogues: CGRO/BATSE, Swift /BAT, Beppo SAX/GRBM, and Insight-HXMT. The statistically significant peaks were identified by means of well tested and calibrated algorithm MEPSA and further selected by applying a set of thresholds on the signal-to-noise ratio. We then extracted the corresponding distributions of number of peaks per GRB. Results. Among the different models considered (power-law, simple or stretched exponential), we find that only a mixture of two exponentials was able to model all the observed distributions. This suggests the existence of two distinct behaviours: (i) an average number of 2.1 ± 0.1 peaks per GRB (“peak-poor”), accounting for about 80% of the observed population of GRBs; and (ii) an average number of 8.3 ± 1.0 peaks per GRB (“peak-rich”), accounting for the remaining 20% of the observed population. Conclusions. We associate the class of peak-rich GRBs with the presence of sub-second variability, which appears to be surprisingly absent among peak-poor GRBs. The two classes could result from two distinct regimes in which the inner engines of GRBs release their energy or otherwise dissipate that energy as gamma rays.
ABSTRACT
We present the S-PLUS Transient Extension Program (STEP): a supernova and fast transient survey conducted in the southern hemisphere using data from the Southern Photometric Local Universe ...Survey (S-PLUS) Main Survey and the T80-South telescope. Transient astrophysical phenomena have a range of interest that goes through different fields of astrophysics and cosmology. With the detection of an electromagnetic counterpart to the gravitational wave (GW) event GW170817 from a binary neutron stars merger, new techniques and resources to study fast astrophysical transients in the multimessenger context have increased. In this paper, we present the STEP overview, the SN follow-up data obtained, data reduction, analysis of new transients and deep learning algorithms to optimize transient candidate selection. Additionally, we present prospects and optimized strategy for the search of gravitational wave counterparts in the current LIGO/Virgo/Kagra observational run (O4) in the context of T80-South telescope.
Reach-to-grasp tasks have become popular paradigms for exploring the neural origin of hand and arm movements. This is typically investigated by correlating limb kinematic with electrophysiological ...signals from intracortical recordings. However, it has never been investigated whether reach and grasp movements could be well expressed in the muscle domain and whether this could bring improvements with respect to current joint domain-based task representations. In this study, we trained two macaque monkeys to grasp 50 different objects, which resulted in a high variability of hand configurations. A generic musculoskeletal model of the human upper extremity was scaled and morphed to match the specific anatomy of each individual animal. The primate-specific model was used to perform 3-D reach-to-grasp simulations driven by experimental upper limb kinematics derived from electromagnetic sensors. Simulations enabled extracting joint angles from 27 degrees of freedom and the instantaneous length of 50 musculotendon units. Results demonstrated both a more compact representation and a higher decoding capacity of grasping tasks when movements were expressed in the muscle kinematics domain than when expressed in the joint kinematics domain. Accessing musculoskeletal variables might improve our understanding of cortical hand-grasping areas coding, with implications in the development of prosthetics hands.
Purpose
Inadequate subscapularis repair has been advocated as one of the contributing factors for dislocation in reverse total shoulder arthroplasty; nonetheless the need to restore the subscapularis ...tendon integrity is under debate. The aim of this systematic review was to answer the question: does subscapularis reattachment following reverse total shoulder arthroplasty improve joint stability, range of motion and functional scores?
Methods
The literature was systematically screened in accordance with PRISMA guidelines looking for papers evaluating clinical outcomes of reverse total shoulder arthroplasty in relation to the management of subscapularis tendon. Studies comparing clinical outcomes, complications and dislocation rate with or without subscapularis repair were included. Studies in which reverse total shoulder arthroplasty was performed for trauma or tumors were excluded. The methodology of included articles was scored with MINORS scale and the Risk of Bias was assessed adopting the ROBINS-I (Risk Of Bias In Non-randomized Studies of Interventions) developed by the Cochrane Group. A meta-analysis was also performed combining the studies to increase the sample size and hence the power to obtain meaningful data.
Results
The database search identified 1062 records, and 6 full-text articles were finally included. A total number of 1085 reverse total shoulder arthroplasty were assessed on. Except for one study, lateralized prosthetic designs have been used. Dislocation occurred in 0.8% (5/599 patients) of the patient with repaired subscapularis and in 1.6% (8/486 patients) of the tenotomized patients, and subscapularis repair was not associated with a higher risk of dislocation (pooled Peto OR: 0.496, 95% CI: 0.163 to 1.510,
p
= 0.217). Qualitative assessment revealed no differences in the range of motion and clinical scores.
Conclusion
Subscapularis repair after reverse total shoulder arthroplasty produces no clinically meaningful benefits, particularly using lateralized prosthetic designs. Subscapularis re-attachment does not improve implant stability, nor increases range of motion or clinical scores. Given these results, keeping in mind the antagonistic effect of the repaired subscapularis on external rotation, no evidence lead to suggest subscapularis reattachment following reverse total shoulder arthroplasty with lateralized prosthetic designs.
Most human spinal cord injuries are anatomically incomplete, leaving some fibers still connecting the brain with the sublesional spinal cord. Spared descending fibers of the brainstem motor control ...system can be activated by deep brain stimulation (DBS) of the cuneiform nucleus (CnF), a subnucleus of the mesencephalic locomotor region (MLR). The MLR is an evolutionarily highly conserved structure which initiates and controls locomotion in all vertebrates. Acute electrical stimulation experiments in female adult rats with incomplete spinal cord injury conducted in our lab showed that CnF-DBS was able to re-establish a high degree of locomotion five weeks after injury, even in animals with initially very severe functional deficits and white matter lesions up to 80-95%. Here, we analyzed whether CnF-DBS can be used to support medium-intensity locomotor training and long-term recovery in rats with large but incomplete spinal cord injuries. Rats underwent rehabilitative training sessions three times per week in an enriched environment, either with or without CnF-DBS supported hindlimb stepping. After 4 weeks, animals that trained under CnF-DBS showed a higher level of locomotor performance than rats that trained comparable distances under non-stimulated conditions. The MLR does not project to the spinal cord directly; one of its main output targets is the gigantocellular reticular nucleus in the medulla oblongata. Long-term electrical stimulation of spared reticulospinal fibers after incomplete spinal cord injury via the CnF could enhance reticulospinal anatomical rearrangement and in this way lead to persistent improvement of motor function. By analyzing the spared, BDA-labeled giganto-spinal fibers we found that their gray matter arborization density after discontinuation of CnF-DBS enhanced training was lower in the lumbar L2 and L5 spinal cord in stimulated as compared to unstimulated animals, suggesting improved pruning with stimulation-enhanced training. An on-going clinical study in chronic paraplegic patients investigates the effects of CnF-DBS on locomotor capacity.
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