This study provides updated information on the distribution of the phlebotomine sand fly species and their genetic characterization in Sardinia, a Mediterranean island where leishmaniasis is endemic. ...From April to November 2017, sand flies were trapped in five different capture sites using sticky traps and light traps as collection methods, operated nearby sheep, poultry, cat, and dog shelters. Phlebotomine specimens (
n
= 513) collected were morphologically and molecularly identified as
Phlebotomus perniciosus
(249/513, 48.5%),
Phlebotomus perfiliewi
(236/513; 46%), and
Sergentomyia minuta
(28/513, 5.5%). Sand flies were collected from the second half of May to October confirming the well-defined seasonal activity, which peaks in August in Sardinia. Overall, correlation analyses indicated a significant positive association between the monthly number of sand flies collected and the mean temperature (
r
= 0.88, rho = 0.87, and tau = 0.69,
P
< 0.05), while there was non-significant, moderately negative correlation between the monthly number of sand flies collected and the monthly mean relative humidity and wind (
r
= − 0.22, rho = 0.02, and tau = 0.04,
P
> 0.05). This study provides the first data on the molecular characterization of phlebotomine sand flies in this region and confirms the presence of three sand fly species. Molecular results suggest that the morphological features used for analysis represent synapomorphic-derived characters which are shared among descendant taxa and the common ancestor.
In addition to the well-known Aelurostrongylus abstrusus (Strongylida: Angiostrongylidae), Troglostrongylus brevior (Strongylida: Crenosomatidae) has recently been diagnosed as a causative agent of ...bronco-pulmonary infections of cats in Spain and Italy. However, information concerning the impact of this species of lungworm on feline population is limited to a few case reports. From July 2011 to May 2013 an epidemiological survey was carried out on Sardinia island (Italy), where 107 individual faecal samples were examined by Baermann technique, and first-stage larvae were identified based on their morphology and characterization of molecular markers. The 29.9% (32/107) of cats examined were infested by broncho-pulmonary nematodes and, although A. abstrusus was the most frequently detected (n=27; 25.2%), larvae of T. brevior were also found (n=7; 6.5%). In addition, two cats (1.9%) were co-infested by both species. Overall metastrongyloid infection was higher in female cats (n=22; 38.6%) than in males (n=10; 20%) (χ2=4.39; p=0.036). The mean age of positive animals was 21.1 (±29.8) months, being infected animals from 2 months to 10 years of age. Of the 32 animals that scored positive for lungworms only 6 (18.8%) displayed a respiratory condition associated with lungworm infestations. Biomolecular characterization confirmed the morphological diagnosis of A. abstrusus. Positive samples that were identified at genus level as Troglostrongylus spp. were molecularly characterized as T. brevior. This study represents the first epidemiological survey on metastrongyloid lungworms of domestic cats in Sardinia and the first report of T. brevior on this island.
BackgroundAlthough rare, human–shark interactions can result in a wide spectrum of injuries. This is the first study to characterize shark-related injuries (SRIs) in Hawai’i.MethodsThis is a ...retrospective review of the State of Hawai’i Division of Aquatic Resources Shark Incidents List between January 1, 2009 and December 31, 2019. Trauma registry data and medical records of patients treated for SRIs at the only level 1 trauma center in Hawai’i were reviewed.ResultsSixty-one patients sustained SRIs in the Hawaiian Islands: 25 in Maui, 16 in O’ahu, 12 in Hawai’i, and 8 in Kaua’i. In cases where the shark species could be identified, tiger sharks were the most frequent (25, 41%). Four cases were fatal—all died on scene in Maui with the shark species unknown. Forty-five survivors (79%) received definitive care at regional facilities. Twelve (21%) were treated at the level 1 trauma center, of which two were transferred in for higher level of care. Of the 12 patients, 11 (92%) had extremity injuries, with 3 lower extremity amputations (25%), 2 with vascular injuries (17%), and 5 with nerve injuries (42%). One had an injury to the abdomen. All patients had local bleeding control in the prehospital setting, with 9 (75%) tourniquets and 3 (25%) hemostatic/pressure dressings applied for truncal or proximal extremity injuries. The mean time from injury to emergency department arrival was 63 minutes.DiscussionMost SRIs are managed at regional facilities, rather than at a level 1 trauma center. Prehospital hemorrhage control is an important survival skill as time to definitive care may be prolonged. For cases treated at the level 1 trauma center, nerve injuries were common and should be suspected even in the absence of major vascular injury. Correlating shark behavior with observed injury patterns may help improve public awareness and ocean safety.Level of evidenceLevel V, epidemiological.
In the last decade, the availability of innovative algorithms derived from complexity theory has inspired the development of highly detailed models in various fields, including physics, biology, ...ecology, economy, and medicine. Due to the availability of novel and ever more sophisticated diagnostic procedures, all biomedical disciplines face the problem of using the increasing amount of information concerning each patient to improve diagnosis and prevention. In particular, in the discipline of orthodontics the current diagnostic approach based on clinical and radiographic data is problematic due to the complexity of craniofacial features and to the numerous interacting co-dependent skeletal and dentoalveolar components. In this study, we demonstrate the capability of computational methods such as network analysis and module detection to extract organizing principles in 70 patients with excessive mandibular skeletal protrusion with underbite, a condition known in orthodontics as Class III malocclusion. Our results could possibly constitute a template framework for organising the increasing amount of medical data available for patients' diagnosis.
The laser-induced release of a well-known hepatoprotective drug (silibinin, SLB) from a temperature-sensitive polymeric composite loaded with silver nanoparticles (Ag NPs) was investigated. The ...surface chemistry tuning and the specific design of Ag NPs are fundamental in view of the engineering of specific stimuli-responsive systems, able to control drug release in response to external stimuli. The release profiles of SLB from the newly synthesized PEG–PLA@Ag composite show strong dependences on laser wavelength and Ag NPs’ Surface Plasmon Resonance (SPR). The resonant laser light excites the SPR of the NPs and the absorbed energy is converted into heat due to electron–photon collisions. The heat generated from the nanometer-sized metal particles embedded within the polymer is efficient and strongly localized. The nanovector, irradiated by a relatively low-intensity laser but tuned specifically to the metal NPs’ SPR, releases the encapsulated drug with a higher efficiency than that not irradiated or irradiated with a laser wavelength far from the metal SPR. A combination of analytical techniques including UV–Vis, NMR, and FT-IR spectroscopy and scanning/transmission electron microscopy has been used to study the structural and morphological properties of the composite. The controllable specificity of this approach and the possibility of the SPR-mediated localized photothermal effect to be usefully applied in aqueous environments are the relevant advances of the proposed system for photothermal therapies that make use of visible optical radiation or for the drug delivery in proximity of the tumor cells.
Although cystic echinococcosis (CE) has been a recognized public health problem in Greece, molecular data are lacking regarding the types and prevalences of infecting strains of the etiological agent ...Echinococcus granulosus. Therefore, we investigated the prevalence of CE and determined the infecting genotypes in sheep and goats in Peloponnesus, a large region of southern Greece. Liver and lung samples were obtained from 210 sheep and 190 goats slaughtered between January and December 2005, and the number, morphology, and fertility of hydatid cysts were determined. Protoscoleces or germinal layers were collected from individual cysts (20 sheep and 20 goats), and DNA was extracted. A polymerase chain reaction (PCR)/seminested PCR system was used to distinguish the G1, G5, and G6/G7 strains, and a specific molecular diagnosis was obtained by sequencing PCR-amplified mitochondrial DNA encoding cytochrome c oxidase subunit 1 and NADH dehydrogenase I genes. The prevalence of CE was 30.4% in sheep and 14.7% in goats; fertile cysts were found in 16.2 and 7.4%, respectively. Overall, 18 of 20 sheep harbored the G1 genotype (common sheep strain), while the remaining two animals had the G3 (buffalo) strain. All 20 goats were infected with the G7 (pig) strain. These results document the prevalence of E. granulosus infection in food animals in this geographical area and reveal for the first time the presence of, at least, three parasite genotypes.
Increased parasympathetic tone achieved with endurance training may provide cardioprotection after menopause. To compare heart rate variability (HRV) from rest through maximal exercise and recovery ...in trained postmenopausal women. Thirty-six postmenopausal women who self-reported training at either moderate (MOD; 3-5.9 METS; 58.9+/-4.4 year) or vigorous (VIG; >6 METS; 59.7+/-5.2 year) intensities participated. HRV was measured for 5 min in the supine position, in the last minute of the VO2max test and after 2 min of active recovery. HRV in MOD and VIG was compared using a factorial ANOVA with repeated measures on time. MOD and VIG responded similarly over the three time periods for root mean square of sequential deviations (rMSSD), and high (HF) and low frequency (LF) power (p>0.05). Maximal exercise lowered rMSSD (3.3+/-0.08 vs. 1.2+/-0.06) and lnLF (4.1+/-0.05 vs. 3.3+/-0.13) and increased lnHF (3.3+/-0.14 vs. 4.0+/-0.10; p<0.01) from resting. However, active recovery restored lnHF (3.3+/-0.11) and lnLF (4.1+/-0.08) from maximal values (p<0.01). Our findings suggest that moderate and vigorous exercise training may enhance HRV recovery following one bout of maximal exercise in older women.
Constant-angle surfaces in liquid crystals Cermelli, P.; Di Scala, A. J.
Philosophical magazine (Abingdon, England),
4/21/2007, Letnik:
87, Številka:
12
Journal Article
Recenzirano
Odprti dostop
We discuss some properties of surfaces in
whose unit normal vector has constant angle with an assigned direction field. The constant-angle condition can be rewritten as a Hamilton-Jacobi equation ...correlating the surface and the direction field. We focus on examples motivated by the physics of interfaces in liquid crystals and of layered fluids, and examine some properties of the constant-angle surfaces when the direction field is singular along a line (disclination) or at a point (hedgehog). We also show how our results may be used to study the shape of disclination cores.
We numerically investigate the supercooled dynamics of two simple model liquids exploiting the partition of the multidimensional configuration space in basins of attraction of the stationary points ...(inherent saddles) of the potential energy surface. We find that the inherent saddle order and potential energy are well-defined functions of the temperature T. Moreover, by decreasing T, the saddle order vanishes at the same temperature (T(MCT)) where the inverse diffusivity appears to diverge as a power law. This allows a topological interpretation of T(MCT): it marks the transition from a dynamics between basins of saddles (T > T(MCT)) to a dynamics between basins of minima (T < T(MCT)).
Burning Mouth Syndrome (BMS) is a chronic pain syndrome that mainly affects middle-aged/old women with hormonal changes or psychological disorders. This condition is probably of multifactorial ...origin, often idiopathic, and its etiopathogenesis remains largely enigmatic. The present paper discusses several aspects of BMS, updates current knowledge, and provides guidelines for patient management. There is no consensus on the diagnosis and classification of BMS. The etiopathogenesis seems to be complex and in a large number of patients probably involves interactions among local, systemic, and/or psychogenic factors. In the remaining cases, new interesting associations have recently emerged between BMS and either peripheral nerve damage or dopaminergic system disorders, emphasizing the neuropathic background in BMS. Based on these recent data, we have introduced the concepts of "primary" (idiopathic) and "secondary" (resulting from identified precipitating factors) BMS, since this allows for a more systematic approach to patient management. The latter starts with a differential diagnosis based on the exclusion of both other orofacial chronic pain conditions and painful oral diseases exhibiting muco-sal lesions. However, the occurrence of overlapping/overwhelming oral mucosal pathologies, such as infections, may cause difficulties in the diagnosis ("complicated BMS"). BMS treatment is still unsatisfactory, and there is no definitive cure. As a result, a multidisciplinary approach is required to bring the condition under better control. Importantly, BMS patients should be offered regular follow-up during the symptomatic periods and psychological support for alleviating the psychogenic component of the pain. More research is necessary to confirm the association between BMS and systemic disorders, as well as to investigate possible pathogenic mechanisms involving potential nerve damage. If this goal is to be achieved, a uniform definition of BMS and strict criteria for its classification are mandatory.
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