Precipitation is a key input variable in distributed surface water‐groundwater models, and its spatial variability is expected to impact watershed hydrologic response via changes in subsurface flow ...dynamics. Gridded precipitation data sets based on gauge observations, however, are plagued by uncertainty, especially in mountainous terrain where gauge networks are sparse. To examine the mechanisms via which uncertainty in precipitation data propagates through a watershed, we perform a series of numerical experiments using an integrated surface water‐groundwater hydrologic model, ParFlow.CLM. The Kaweah River watershed in California, USA, is used as our virtual catchment laboratory to characterize watershed response to variable precipitation forcing from headwaters to groundwaters. By applying the three‐cornered hat method, we quantify the spatially distributed uncertainty in four publically available precipitation forcing data sets and their simulated hydrology. Simulations demonstrate that uncertainty in the simulated groundwater storage is primarily a result of topographic redistribution of uncertainty in precipitation forcing. Soil water redistribution is the primary pathway that redistributes uncertainty downslope. We also find that topography exerts a larger impact than variable subsurface parameters on propagating uncertainty in simulated fluxes. Finally, we find that improvement in model performance metrics is higher for a single simulation forced with the mean precipitation from the available data sets than the averaged simulated results of separate simulations forced with each data set. Results from this study highlight the importance of topography‐moderated flow through the critical zone in shaping the groundwater response to climate variability.
Key Points
Uncertainty in simulated groundwater storage change is a result of topographic redistribution of uncertainty in precipitation forcing
Subsurface flow pathways in both soil and deeper bedrock control watershed response to variable precipitation input
Merging multiple precipitation data sets provides better results than merging simulated fluxes, due to high model sensitivity to changes in precipitation
The ability of animals to sense and differentiate among thousands of odorants relies on a large set of olfactory receptors (OR) and a multitude of accessory proteins within the olfactory epithelium ...(OE). ORs and related signaling mechanisms have been the subject of intensive studies over the past years, but our knowledge regarding olfactory processing remains limited. The recent development of next generation sequencing (NGS) techniques encouraged us to assess the transcriptome of the murine OE. We analyzed RNA from OEs of female and male adult mice and from fluorescence-activated cell sorting (FACS)-sorted olfactory receptor neurons (ORNs) obtained from transgenic OMP-GFP mice. The Illumina RNA-Seq protocol was utilized to generate up to 86 million reads per transcriptome. In OE samples, nearly all OR and trace amine-associated receptor (TAAR) genes involved in the perception of volatile amines were detectably expressed. Other genes known to participate in olfactory signaling pathways were among the 200 genes with the highest expression levels in the OE. To identify OE-specific genes, we compared olfactory neuron expression profiles with RNA-Seq transcriptome data from different murine tissues. By analyzing different transcript classes, we detected the expression of non-olfactory GPCRs in ORNs and established an expression ranking for GPCRs detected in the OE. We also identified other previously undescribed membrane proteins as potential new players in olfaction. The quantitative and comprehensive transcriptome data provide a virtually complete catalogue of genes expressed in the OE and present a useful tool to uncover candidate genes involved in, for example, olfactory signaling, OR trafficking and recycling, and proliferation.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Channel transmission losses alter the streamflow response of arid and semiarid watersheds and promote focused groundwater recharge. This process has been primarily studied in dryland channels ...draining large areas that are displaced away from hillslope runoff generation. In contrast, small watersheds on arid piedmont slopes allow the investigation of interactive hillslope and channel processes that control the partitioning between surface and subsurface flows. In this study, we utilize high‐resolution, long‐term measurements of water balance components in an instrumented watershed of the Chihuahuan Desert to set up, parameterize, and test a process‐based, distributed hydrologic model modified to account for channel losses. A transient method for capturing capillary effects in channels results in simulations with a reliable representation of the watershed energy balance, soil moisture dynamics, hillslope infiltration, channel transmission (or percolation) losses, and streamflow yield over the study period. The simulation also reproduces a conceptual model of hillslope infiltration‐excess runoff generation linked to downstream channel percolation losses that depend on the rainfall event size. Model‐derived thresholds were obtained for the amount of hillslope runoff (6 mm) and rainfall (12.5 mm) necessary for streamflow yield, such that 40% of percolation occurs for small events that do not reach the outlet. Using a set of scenarios, we identify that hillslope infiltration controls the rainfall threshold necessary to initiate percolation, while channel infiltration affects the partitioning into percolation and streamflow yield. Thus, the connectivity along hillslope‐channel pathways is deemed an essential control on the streamflow generation and groundwater recharge in arid regions with complex terrain.
Key Points
A distributed hydrologic model is tested with long‐term measurements of water and energy states and fluxes in a piedmont slope watershed
When modified to account for transient channel losses, the model reproduces well the percolation estimates and observed streamflow response
Modeling scenarios reveal the relative importance of hillslope and channel properties on runoff generation and percolation losses
Methane non‐oxidative coupling to ethylene was investigated on Mo2C/BZSM‐5 catalyst at 923 K and atmospheric pressure. In contrast to Mo2C/AlZSM‐5 catalysts for methane aromatization, this material ...exhibits very high ethylene selectivity (>90 %) and low aromatics (benzene and naphthalene) selectivity. The much weaker Brønsted acidity of BZSM‐5 leads to a slow rate of ethylene oligomerization. The stability of the catalyst is greatly enhanced with 93 % of the initial reaction rate remaining after 18 h of time on stream. In‐situ UV/VIS spectra indicate that prior to carburization, mono/binuclear Mo oxides are initially well dispersed onto the zeolite support. Mo carbides clusters, formed during carburization with methane, appear similar to clusters formed in AlZSM‐5, as indicated by the X‐ray Absorption Spectroscopy (XAS) data.
Selective zeolite: A highly selective methane non‐oxidative coupling catalyst Mo2C/BZSM‐5 is synthesized and characterized. Boron is employed instead of aluminium in the zeolite framework, which leads to a dramatic decrease in the zeolite acidity. Due to the low acidity of the support, the catalyst shows a high selectivity towards ethylene (≈90 %) instead of aromatics.
The specific functions of sensory systems depend on the tissue-specific expression of genes that code for molecular sensor proteins that are necessary for stimulus detection and membrane signaling. ...Using the Next Generation Sequencing technique (RNA-Seq), we analyzed the complete transcriptome of the trigeminal ganglia (TG) and dorsal root ganglia (DRG) of adult mice. Focusing on genes with an expression level higher than 1 FPKM (fragments per kilobase of transcript per million mapped reads), we detected the expression of 12984 genes in the TG and 13195 in the DRG. To analyze the specific gene expression patterns of the peripheral neuronal tissues, we compared their gene expression profiles with that of the liver, brain, olfactory epithelium, and skeletal muscle. The transcriptome data of the TG and DRG were scanned for virtually all known G-protein-coupled receptors (GPCRs) as well as for ion channels. The expression profile was ranked with regard to the level and specificity for the TG. In total, we detected 106 non-olfactory GPCRs and 33 ion channels that had not been previously described as expressed in the TG. To validate the RNA-Seq data, in situ hybridization experiments were performed for several of the newly detected transcripts. To identify differences in expression profiles between the sensory ganglia, the RNA-Seq data of the TG and DRG were compared. Among the differentially expressed genes (> 1 FPKM), 65 and 117 were expressed at least 10-fold higher in the TG and DRG, respectively. Our transcriptome analysis allows a comprehensive overview of all ion channels and G protein-coupled receptors that are expressed in trigeminal ganglia and provides additional approaches for the investigation of trigeminal sensing as well as for the physiological and pathophysiological mechanisms of pain.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Argonaute (AGO) proteins partner with microRNAs (miRNAs) to target specific genes for post-transcriptional regulation. During larval development in Caenorhabditis elegans, Argonaute-Like Gene 1 ...(ALG-1) is the primary mediator of the miRNA pathway, while the related ALG-2 protein is largely dispensable. Here we show that in adult C. elegans these AGOs are differentially expressed and, surprisingly, work in opposition to each other; alg-1 promotes longevity, whereas alg-2 restricts lifespan. Transcriptional profiling of adult animals revealed that distinct miRNAs and largely non-overlapping sets of protein-coding genes are misregulated in alg-1 and alg-2 mutants. Interestingly, many of the differentially expressed genes are downstream targets of the Insulin/ IGF-1 Signaling (IIS) pathway, which controls lifespan by regulating the activity of the DAF-16/ FOXO transcription factor. Consistent with this observation, we show that daf-16 is required for the extended lifespan of alg-2 mutants. Furthermore, the long lifespan of daf-2 insulin receptor mutants, which depends on daf-16, is strongly reduced in animals lacking alg-1 activity. This work establishes an important role for AGO-mediated gene regulation in aging C. elegans and illustrates that the activity of homologous genes can switch from complementary to antagonistic, depending on the life stage.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Water returned to the atmosphere as evapotranspiration (ET) is approximately 1.6x global river discharge and has wide‐reaching impacts on groundwater and streamflow. In the U.S. Midwest, widespread ...land conversion from prairie to pasture to cropland has altered spatiotemporal patterns of ET, yet there is not consensus on the direction of change or the mechanisms controlling changes. We measured ET at three locations within the Long‐Term Agroecosystem Research network along a latitudinal gradient with paired rainfed cropland and prairie sites at each location. At the northern locations, the Upper Mississippi River Basin (UMRB) and Kellogg Biological Station (KBS), the cropland has annual ET that is 84 and 29 mm/year (22% and 5%) higher, respectively, caused primarily by higher ET during springtime when fields are fallow. At the southern location, the Central Mississippi River Basin (CMRB), the prairie has 69 mm/year (11%) higher ET, primarily due to a longer growing season. Differences in climate and that the CMRB prairie is remnant native prairie, while the UMRB and KBS prairies are restored, make it challenging to attribute differences to specific mechanisms. To accomplish this, we examine the energy balance using the Two‐Resistance Method (TRM). Results from the TRM demonstrate that higher surface conductance in croplands is the primary factor leading to higher springtime ET from croplands, relative to prairies. Results from this study provide insight into impacts of warm season grasses on the hydrology of the U.S. Corn Belt by providing a mechanistic understanding of how land use change affects the water budget.
Plain Language Summary
Evapotranspiration (ET) consists of evaporation from bare soil and plant leaves. ET is ∼1.6x greater than global river flow and has wide‐reaching impacts on groundwater and streamflow. In the U.S. Midwest, widespread land conversion from prairies to croplands has altered patterns of ET, yet there is no consensus on the direction of this change or the mechanisms controlling changes. In this study we use measurements of ET at three locations within the Long‐Term Agroecosystem Research (LTAR) network that have paired cropland and prairie sites. Surprisingly, we found that in the two northern sites, the croplands had higher ET than the prairies, particularly during springtime when the croplands are fallow. We used mathematical analysis of the energy budget to show that a parameter called the surface conductance controls the differences in ET between the croplands and prairies. During springtime in prairies, the standing, dormant vegetation blocks transfer of water vapor from the land surface, reducing the surface conductance, and limits the ET. Results from this study provide insight into the impact of land conversion from prairies to croplands on the hydrology of the U.S. Corn Belt by providing a mechanistic understanding of how land use change affects the water budget.
Key Points
Differences in evapotranspiration between croplands and prairies was quantified by a mechanistic Two Resistance Method
Bowen ratio during springtime is higher in prairies than croplands
Surface resistance is the primary factor causing springtime evapotranspiration differences between croplands and prairies
Accurate simulation of plant water use across agricultural ecosystems is essential for various applications, including precision agriculture, quantifying groundwater recharge, and optimizing ...irrigation rates. Previous approaches to integrating plant water use data into hydrologic models have relied on evapotranspiration (ET) observations. Recently, the flux variance similarity approach has been developed to partition ET to transpiration (T) and evaporation, providing an opportunity to use T data to parameterize models. To explore the value of T/ET data in improving hydrologic model performance, we examined multiple approaches to incorporate these observations for vegetation parameterization. We used ET observations from five eddy covariance towers located in the irrigated San Joaquin Valley, California, to parameterize orchard crops in an integrated land surface—groundwater model. By using ET, or both ET and T data, we examined the impact of multiple model parameterization approaches ranging from simple performance metrics to the generalized likelihood uncertainty estimation method. We find that a simple approach of selecting the parameter sets based on ET and T performance metrics works best at these study sites. Selecting parameters based on performance relative to observed ET creates an uncertainty of 27% relative to the observed value. When parameters are selected using both T and ET data, this uncertainty drops to 24%. Similarly, the uncertainty in potential groundwater recharge drops from 63% to 58% when parameters are selected with ET or T and ET data, respectively. While these improvements are minor in an irrigated setting, the value of partitioning ET data may be more useful in non‐irrigated settings. Additionally, using crop type parameters results in similar levels of simulated ET as using site‐specific parameters. Different irrigation schemes create high amounts of uncertainty and highlight the need for accurate estimates of irrigation when performing water budget studies.
Accurate simulation of plant water use across agricultural ecosystems is essential for various applications, including precision agriculture, quantifying groundwater recharge and optimizing irrigation rates. We use eddy covariance measurements and partition evapotranspiration into plant water use and bare soil evaporation to improve parameterization of vegetation in a land surface model. We test approaches to incorporate this data and quantify the uncertainty in the simulated water budget.
•Investigation on the thermal behavior of different designs of PCM packages included in concrete blocks.•Changing the PCM design enables a desired adjustment of the thermal behavior of concrete ...blocks.•Thin and set up positioned PCM layers generate fast crystallization and melting times compared to massive PCM blocks.•Flat overlaid PCM packages generate a thermal shading effect leading to longer melting and crystallization periods.•Macroencapsulated PCM packages can be positioned to achieve the most positive thermal effect.
Most installed phase change materials in real applications are typically spherical microencapsulated paraffin. However, salt hydrates can be a cheap alternative when combined with a suitable macroencapsulation. Such macroencapsulation enables the installation of different shapes and positions within the building material. Therefore, an experiment setup consisting of a wooden box equipped with infrared radiators and a cooler was used to investigate cuboid, cylindrical, plate-shaped, and spherical phase-change packages. All water vapor impermeable packages filled with salt hydrate were positioned within a standard concrete block. As a result, it can be seen that changing the design of a PCM package enables the opportunity to adjust the thermal behavior without changing the mass of the PCM. For a high heat transfer, a set up position of a large number of thin packages with a large heat transfer surface was found to be the best design. Using a thermal shading effect and an even distribution of the PCM within concrete block ensures a slow increase in the temperature of the block and thus a lower heat flow into the inner rooms.
This paper presents a mathematical model for designing a carbon dioxide (CO2) value chain. Storage of CO2 in geological formations is recognized as an important alternative for carbon abatement. When ...CO2 is deposited in oil reservoirs it can sometimes be used to achieve additional oil production, enhanced oil recovery (EOR). The model determines an optimal CO2 value chain from a fixed set of CO2 emission points and a set of potential injection sites. It designs a transport network and chooses the best suited oil fields with EOR potential or other geological formations for storage. A net present value criterion is used. The model is illustrated by an example of a Norwegian case with 14 oil fields, two aquifers and five CO2 sources. A sensitivity analysis is performed on the most important parameters.