The production of eluates for testing acute cytotoxicity by a new method Schubert, T; Hegner, S; Bergner, J
Zeitschrift fur experimentelle Chirurgie, Transplantation, und kunstliche Organe : Organ der Sektion Experimentelle Chirurgie der Gesellschaft fur Chirurgie der DDR,
1989, Letnik:
22, Številka:
2
Journal Article
In vitro tests of new biomaterials require internationally and nationally eluates increasingly. Only the extracted substances become effective in the cells in that way. The material surface has the ...greatest influence on the quantity of the released substances by elution. The surface of diverse undefined mixture quantities is definable exactly by means of a gas adsorption method (BET method). The influence of the secondary technologies was tested by the atom adsorption spectrometry. The Jena bioglass ceramics could be estimated successfully by this method.
The glass crystallization method is shown to be suitable for preparing BaFe(12-2x)Co(x)Ti(x)O(19) powders with a nearly perfect crystallographic structure and a narrow particle size distribution. The ...dependences of the specific magnetization, the coercivity, and the particle size distribution on both the annealing temperature and the Co-Ti content correlate in a characteristic way. Furthermore, the lattice parameters, the specific surfaces and the thickness of the effective nonmagnetic surface layer were determined. The coercivity of the hexaferrite magnetic tapes is more than 8x10(3) A/m higher than for the corresponding powders
The glass crystallization method is shown to be suitable for preparing BaFe sub(12-2x)Co sub(x)Ti sub(x)O sub(19) powders with a nearly perfect crystallographic structure and a narrow particle size ...distribution. The dependence of the specific magnetization, the coercivity, and the particle size distribution on both the annealing temperature and the Co-Ti content correlate in a characteristic way. The coercivity of the hexaferrite magnetic tapes is more than 8 x 10 super(3) A/m higher than for the the corresponding powders.
In this thesis the influence of pneumo-vagina on the microbiological colonization of the genital tract and their manifestation in cytological smears was examined. For mares with poor vulval ...conformation a comparison of the bacterial growth before and after plastic surgery of the vulva and vestibulum was carried out, as well as the registration of conception rates after operation and insemination. The biggest reduction of the bacterial content in the reproductive tract was found between vestibulum and cranial section of the vagina. The increased number of contaminant bacteria in the cranial section of the vagina during abnormal vulval conformation could be reduced significantly by the plastic surgery of the vulva and vestibulum (p < 0.05). Assuming that an 18-20 days pregnant uterus is free from a colonization with virulent bacteria, a high significant reduction of the increased number of contaminant bacteria in the uterus during vulval incompetence was found after surgical correction (p < 0.01). In mares with poor conformation of the vulva, which had not been operated, the contamination of the reproductive tract had increased at the time of checkup in comparison to the first swab specimens, taken 18-20 days before. The share of mares with a negative bacteriological and positive cytological result was in case of vulval incompetence twice as large as if they had a proper vulval conformation. A decrease of these latent infections was found after plastic surgery of the vulva and vestibulum. From the operated mares a total of 72.9% became pregnant.
One hundred twenty-seven patients with endoscopically diagnosed active duodenal, pyloric, or prepyloric ulcers participated in this multicenter, double-blind, randomized, controlled trial comparing ...placebo with enprostil 35 micrograms twice daily for up to four weeks. Cumulative endoscopic healing for the enprostil and placebo treatment groups, respectively, was 25% (15 of 59) and 12% (7 of 60) at two weeks (P = 0.060) and 59% (34 of 58) and 33% (19 of 57) at four weeks (P = 0.005). Excluding prepyloric ulcers, cumulative healing for the enprostil and placebo groups, respectively, was 22% (9 of 41) and 7% (3 of 44) at two weeks (P = 0.104) and 56% (23 of 41) and 24% (10 of 42) at four weeks (P = 0.002). A greater percentage of prepyloric ulcers healed on enprostil than placebo, but the difference was not significant. Mean antacid use in both groups was identical, averaging only two or less tablets per day in each group throughout the study. Daytime pain was relieved more quickly in the enprostil group, while median time to relief of nighttime pain was essentially identical in both groups. The most common side effect in the enprostil treatment group, diarrhea, was mostly mild to moderate in intensity and was generally self-limiting, requiring no specific therapy; no patient withdrew because of this complaint. Other symptoms and laboratory profiles were similar in the two groups. These results indicate that enprostil 35 micrograms taken twice daily for four weeks is effective and safe for the treatment of prepyloric, pyloric channel, and duodenal ulcers.
Fragestellung:
Bei den wenigen Berichten über Patienten mit Morquio-Syndrom (Mucopolysaccharidose Typ IV) werden zumeist nur klinische und radiologische Befunde beschrieben. Nach unserem Wissen ...wurden arthroskopische Befunde bislang nicht veröffentlicht.
Methodik:
Anhand eines Fallberichtes sollen klinische und radiologische, v.a. aber auch arthroskopische und histologische Veränderungen einer 32jährigen Patientin mit Morquio-Syndrom demonstriert werden, bei welcher innerhalb von sechs Monaten beide Kniegelenke arthroskopiert wurden.
Ergebnisse:
Intraoperativ zeigte sich in beiden Kniegelenken bei makroskopisch intakter Gelenkknorpeloberfläche eine großflächige Ablösung des Gelenkknorpels von der subchondralen Knochenschicht. In beiden Kniegelenken waren alle Gelenkkompartimente gleichermaßen von dieser Unterminierung der Knorpelschicht betroffen. In der lichtmikroskopischen Untersuchung einer Knorpel-Knochen-Stanze zeigte der hyaline Knorpel eine regelrechte Dichte der Chondrone sowie eine unauffällige Anfärbung der Interzellularsubstanz. Auffällig war eine breite Bindegewebsschicht zwischen Knorpel- und subchondraler Knochenschicht mit einzelnen, versprengten Knorpelinseln.
Schlussfolgerung:
Zumeist wird eine diffuse Knorpel- und Knochengewebeschädigung durch die Keratansulfatablagerungen beim Morquio-Syndrom beschrieben. Angesichts der dargestellten Befunde sollten unserer Meinung nach jedoch weitere Untersuchungen v.a. auch der osteochondralen Grenzzone erfolgen.
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