Most human hantavirus infections occur in Asia, but some cases have been described in Europe in travelers returning from Asia. We describe a case of hantavirus pulmonary syndrome in a previously ...healthy traveler occurring shortly after he returned to Spain from Nepal. Serologic tests suggested a Puumala virus-like infection.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, ODKLJ, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Cardiac troponin I concentrations in horses with colic Díaz, Olga M Seco; Durando, Mary M; Birks, Eric K ...
Journal of the American Veterinary Medical Association,
2014-Jul-01, Letnik:
245, Številka:
1
Journal Article
Recenzirano
Odprti dostop
To determine prevalence of myocardial injury in horses with colic on the basis of high concentrations of cardiac troponin I (cTnI), frequency of cardiac arrhythmias within the first 24 to 48 hours ...after hospital admission or surgery because of colic, and associations between high cTnI concentrations and cardiac arrhythmias, clinical course, and outcome (survival to discharge from hospital vs nonsurvival death or euthanasia).
Prospective observational study.
111 horses with colic.
Blood was drawn at admission and 12 and 24 hours after admission if horses were treated medically or 12 and 24 hours after surgery if treated surgically. A 24-hour ambulatory ECG was recorded beginning the morning after admission in medically treated cases or after surgery and evaluated for arrhythmias. Clinical and clinicopathologic data and outcome were obtained. Associations between cTnI concentrations and other variables were determined.
An abnormal cTnI concentration (≥ 0.10 ng/mL) at admission was significantly associated with the occurrence of ventricular arrhythmias, outcome, and surgical treatment.
The data suggested that horses with colic and high cTnI concentrations at admission were more likely to have ventricular arrhythmias and have a less favorable prognosis for recovery. High cTnI concentrations in horses with colic were suggestive of myocardial damage.
A family of Mn(
ii
)Ln(
iii
) dinuclear and tetranuclear complexes (Ln = Gd and Dy) has been prepared from the compartmental ligands
N
,
N
′-dimethyl-
N
,
N
...′-bis(2-hydroxy-3-formyl-5-bromobenzyl)ethylenediamine (H
2
L
1
) and
N
,
N
′,
N
′′-trimethyl-
N
,
N
′′-bis(2-hydroxy-3-methoxy-5-methylbenzyl)diethylenetriamine (H
2
L
2
). The Mn(
ii
)Gd(
iii
) complexes exhibit antiferromagnetic interactions between Mn(
ii
) and Gd(
iii
) ions in most cases, which are supported by Density Functional Theory (DFT) calculations. Experimental magneto-structural correlations carried out for the reported complexes and other related complexes found in bibliography show that the highest ferromagnetic coupling constants are observed in di-μ-phenoxido bridged complexes, which is due to the planarity of the Mn-(μ-O)
2
-Gd bridging fragment and to the high Mn-O-Gd angles. The effect of these angles has been studied by DFT calculations performed on a di-μ-phenoxido doubly bridged model. The magneto-thermal properties of the Mn(
ii
)Gd(
iii
) based complexes have also been measured, concluding that the magnitude of the Magneto-Caloric Effect (MCE) is due to the strength rather than to the nature of the magnetic coupling. Moreover, when two Mn(
ii
)Gd(
iii
) dinuclear units are connected by two carbonato-bridging ligands the MCE is enhanced, obtaining a maximum magnetic entropy change of 36.4 Jkg
−1
K
−1
at Δ
B
= 7 T and
T
= 2.2 K. On the other hand, one of the dinuclear Mn(
ii
)Dy(
iii
) complexes displays Single-Molecule Magnet (SMM) behaviour with an energy barrier of 14.8 K under an applied external field of 1000 Oe.
Magneto-structural correlations reveal the basis to design Mn(
ii
) and lanthanide-based complexes with magneto-caloric effect or slow relaxation of the magnetization.
Carbapenem-resistant (CR) sequence type 258 (ST258)
has become an urgent health care threat, causing an increasing number of high-mortality infections. Its resistance to numerous antibiotics and ...threat to immunocompromised patients necessitate finding new therapies to combat these infections. Previous successes in the laboratory, as well as the conservation of capsular polysaccharide (CPS) among the members of the ST258 clone, suggest that monoclonal antibody (MAb) therapy targeting the outer polysaccharide capsule of
could serve as a valuable treatment alternative for afflicted patients. Here, we isolated several IgG antibodies from mice inoculated with a mixture of CR
CPS conjugated to anthrax protective antigen. Two of these MAbs, 17H12 and 8F12, bind whole and oligosaccharide epitopes of the CPS of clade 2 ST258 CR
, which is responsible for the most virulent CR
infections in the United States. These antibodies were shown to agglutinate all clade 2 strains and were also shown to promote extracellular processes killing these bacteria, including biofilm inhibition, complement deposition, and deployment of neutrophil extracellular traps. Additionally, they promoted opsonophagocytosis and intracellular killing of CR
by human-derived neutrophils and cultured murine macrophages. Finally, when mice were intratracheally infected with preopsonized clade 2 CR
, these MAbs reduced bacterial dissemination to organs. Our data suggest that broadly reactive anticapsular antibodies and vaccines against clade 2 ST258 CR
are possible. Such MAbs and vaccines would benefit those susceptible populations at risk of infection with this group of multidrug-resistant bacteria.
Carbapenem-resistant
is an enteric bacterium that has been responsible for an increasing number of deadly outbreaks and hospital-acquired infections. The pathogen's resistance to numerous antibiotics, including new drugs, leaves few therapeutic options available for infected patients, who often are too sick to fight the infection themselves. Immunotherapy utilizing monoclonal antibodies has been successful in other medical fields, and antibodies targeting the outer polysaccharide capsule of these bacteria could be a valuable treatment alternative. This study presents two anticapsular antibodies, 17H12 and 8F12, that were found to be protective against the most virulent carbapenem-resistant
clinical strains. These antibodies are shown to promote the killing of these strains through several extracellular and intracellular processes and prevent the spread of infection in mice from the lungs to distal organs. Thus, they could ultimately treat or protect patients infected or at risk of infection by this multidrug-resistant bacterium.
Four new compounds based on zinc(ii) or cadmium(ii) metal ions and elongated dicarboxylate and bipyridine ligands, namely, {Cd sub(3)( mu sub(4)-bpdc) sub(3)(H sub(2)O) sub(2).DMF} sub()n(1), {Zn ...sub(3)( mu sub(4)-bpdc) sub(3)( mu -bpdb).5DMF} sub()n(2), {Zn sub(2)( mu sub(4)-bpdc) sub(2)( mu -bpdb).7DMF} sub()n(3), and {Zn sub(4)( mu sub(4)-bpdc) sub(3)(DMF)( mu sub(4)-O)(H sub(2)O).7DMF.3H sub(2)O} sub()n(4), (where bpdc = biphenyl-4,4'-dicarboxylate, bpdb = 1,4-bis(4-pyridyl)-2,3-diaza-1,3-butadiene, DMF = dimethylformamide) have been synthesised under solvothermal conditions and structurally characterised by single crystal X-ray diffraction. The crystal structures range from 2D (in 1) to 3D (2, 3, and 4) systems according to the coordination mode acquired by the bpdc ligand and the presence of an ancillary linker. Compound 1 consists of stacked Cd-bpdc neutral layers containing isolated small voids. The coordination of the bpdb ligand (2 and 3) or the formation of a tetrahedral Zn sub(4)O cluster (in 4) generates highly open 3D architectures that share the structural feature of being doubly interpenetrated. A careful computational analysis on the crystal structures permits unravelling their void systems. Moreover, characterising the photoluminescence emission of the compounds at variable excitation wavelengths provides an opportunity to couple the luminescence response with their porosity, which could signify the potential utility of these materials as photofluorescent sensors for small adsorbates.
We report herein the synthesis and structural and chemical characterization of six new compounds consisting of 6-aminonicotinate (6ani) ligands and first-row transition metal ions, namely, Mn2( mu ...-6ani)2(H2O)8(6ani)2 (1), Co(6ani)(bipy)2(H2O)3(6ani).5H2O (2), M( mu -6ani)(6ani)(H2O)3.2H2O MII = Co (3), Ni (4), and M( mu -6ani)2.H2O MII = Co (5), Cu (6) (where bipy = 4,4'-bipyridine). Compounds 1 and 2 consist of isolated dimeric and monomeric entities held together by supramolecular interactions governed by 6ani free anions. The rigid and low symmetry of 6ani ligands, in addition to their coordination as terminal and bridging ligands to octahedral metal geometries, gives rise to chiral 1D chains of compounds 3 and 4. 2D sql layers are formed (5 and 6) when 6ani ligands act as ligands bridging the metal ions, which precludes the presence of water molecules in the framework. Despite the wide structural diversity observed, all architectures share the occurrence of magnetically isolated 3d metal ions, given the poor exchange achieved through 6ani bridges, as confirmed by dc susceptibility measurements and DFT calculations. Dynamic ac susceptibility measurements reveal best-in-class field-induced slow magnetic relaxation behaviour in Co-based compounds with easy-plane magnetic anisotropy. The effective energy barriers (Ueff) of 39.6 K and 18.7 K for 3 and 5, respectively, estimated using the Arrhenius law are remarkably high among CoII-SIM coordination polymers reported so far.
Flaviviruses are a widespread and numerous group of arboviruses that can cause serious illness in humans. The continuous and slow spread of certain flaviviruses, such as Dengue viruses, and the ...recent entry and spread of West Nile virus to the American continent, point to the need to control these infections. This control requires the use of suitable techniques for diagnostic and surveillance programmes. A generic RT-nested-PCR that is, theoretically, able to detect each member of the group has been designed. The identification of the detected virus is carried out by sequencing. The introduction of an internal control would reduce the number of false negative results and could be used to quantify the viral load in clinical samples where the method works well.
Here we report copolymers where the “Sergeants and Soldiers effect” can be switched ON and OFF by the presence of a metal ion. These copolymers have been prepared by a combination of achiral and ...chiral monomer units, where the chiral ones are unable to drive the chiral amplification unless a small amount of mono- or divalent metal ions is added. In this way, the ions act as promoters upgrading some of the chiral pendants, initially unable to induce a preferential helical sense, to a higher rank forcing the arrangement of the rest of the chain in a specific helical sense. In these copolymers, the classical “Sergeant” and “Soldier” roles are modified in such a way that the chiral units become “Sergeants” only by the effect of an achiral external stimulus, namely the metal ion. The structure of the metal complex determines its interaction in the helix with the surrounding chiral and achiral “Soldiers” and therefore also determines both the intensity of the amplification and the response of a copolymer to a certain metal. For instance, poly( 1 r - co - 7 (1−r) ) shows chiral amplification (“Sergeants and Soldiers effect”) towards the right-handed helix only with divalent ions, while poly( 1 r - co - 8 (1−r) ) amplifies the helix to the left-handed sense with mono- and to the right-handed sense with divalent ions. This behaviour allows, using a single copolymer, to selectively induce any of the two helical senses. The aggregation and encapsulation properties of these copolymers are also described.
The mechanism of carbamate activation promoted by different metal ions has been explored in this work. The reaction of the carbamate ligand H
2
L with chloride metal salts (M = Ni, Cu, Zn, Cd) leads ...to the coordination of the metal ions to the ligand, causing hydrolysis of the systems. This self-immolation process results in mononuclear dihydrazone complexes, carbon dioxide and the release of alcohol species from the pendant groups of the carbamate ligand. The conditions under which this process occurs have been studied in detail.
The coordination of metal ions to a carbamate ligand triggers its hydrolysis, which ends up in self-immolation of the complex releasing new metal species, a pendant molecule (A) and CO
2
.
Summary
The probiotic strain Escherichia coli Nissle 1917 (EcN) is a common bacterial chassis in synthetic biology developments for therapeutic applications given its long track record of safe ...administration in humans. Chromosomal integration of the genes of interest (GOIs) in the engineered bacterium offers significant advantages in genetic stability and to control gene dose, but common methodologies relying on the transformation of EcN are inefficient. In this work, we implement in EcN the use of bacterial conjugation in combination with markerless genome engineering to efficiently insert multiple GOIs at different loci of EcN chromosome, leaving no antibiotic resistance genes, vector sequences or scars in the modified bacterium. The resolution of cointegrants that leads to markerless insertion of the GOIs requires expression of I‐SceI endonuclease and its efficiency is enhanced by λ Red proteins. We show the potential of this strategy by integrating different genes encoding fluorescent and bioluminescent reporters (i.e. GFP, mKate2, luxCDABE) both individually and sequentially. We also demonstrate its application for gene deletions in EcN (ΔflhDC) and to replace the endogenous regulation of chromosomal locus (i.e. flhDC) by heterologous regulatory elements (e.g. tetR‐Ptet) in order to have an ectopic control of gene expression in EcN with an external inducer to alter bacterial behaviour (e.g. flagellar motility). Whole‐genome sequencing confirmed the introduction of the designed modifications without off‐target alterations in the genome. This straightforward approach accelerates the generation of multiple modifications in EcN chromosome for the generation of living bacterial therapeutics.
In this work, we implement in the probiotic strain Escherichia coli Nissle 1917 (EcN) the use of bacterial conjugation in combination with markerless genome engineering to efficiently insert multiple GOIs at different loci of EcN chromosome, leaving no antibiotic resistance genes, vector sequences, or scars in the modified bacterium. This straightforward approach accelerates the generation of multiple modifications in EcN chromosome for the generation of living bacterial therapeutics.
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