The cross section of the process e + e − → π + π − has been measured in the center-of-mass energy range from 0.32 to 1.2 GeV with the CMD-3 detector at the electron-positron collider VEPP-2000. The ...measurement is based on an integrated luminosity of about 88 pb − 1 , of which 62 pb − 1 represent a complete dataset collected by CMD-3 at center-of-mass energies below 1 GeV. In the dominant region near the ρ resonance a systematic uncertainty of 0.7% was achieved. The implications of the presented results for the evaluation of the hadronic contribution to the anomalous magnetic moment of the muon are discussed. Published by the American Physical Society 2024
The SEMA3B gene is located in the 3p21.3 LUCA region, which is frequently affected in different types of cancer. The objective of our study was to expand our knowledge of the SEMA3B gene as a tumor ...suppressor and the mechanisms of its inactivation. In this study, several experimental approaches were used: tumor growth analyses and apoptosis assays in vitro and in SCID mice, expression and methylation assays and other. With the use of the small cell lung cancer cell line U2020 we confirmed the function of SEMA3B as a tumor suppressor, and showed that the suppression can be realized through the induction of apoptosis and, possibly, associated with the inhibition of angiogenesis. In addition, for the first time, high methylation frequencies have been observed in both intronic (32-39%) and promoter (44-52%) CpG-islands in 38 non-small cell lung carcinomas, including 16 squamous cell carcinomas (SCC) and 22 adenocarcinomas (ADC), and in 83 clear cell renal cell carcinomas (ccRCC). Correlations between the methylation frequencies of the promoter and the intronic CpG-islands of SEMA3B with tumor stage and grade have been revealed for SCC, ADC and ccRCC. The association between the decrease of the SEMA3B mRNA level and hypermethylation of the promoter and the intronic CpG-islands has been estimated in renal primary tumors (P < 0.01). Using qPCR, we observed on the average 10- and 14-fold decrease of the SEMA3B mRNA level in SCC and ADC, respectively, and a 4-fold decrease in ccRCC. The frequency of this effect was high in both lung (92-95%) and renal (84%) tumor samples. Moreover, we showed a clear difference (P < 0.05) of the SEMA3B relative mRNA levels in ADC with and without lymph node metastases. We conclude that aberrant expression and methylation of SEMA3B could be suggested as markers of lung and renal cancer progression.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Recent Progress in the VEPP-2000 Collider Rastigeev, S. A.; Aktershev, Yu. S.; Bedarev, E. V. ...
Physics of particles and nuclei letters,
08/2023, Letnik:
20, Številka:
4
Journal Article
Recenzirano
The VEPP-2000 is an electron–positron collider with round beams built and operating at the Budker Institute of Nuclear Physics (BINP). The collider luminosity was increased twofold last year. The ...integrated luminosity accumulated over the last year has exceeded 0.3 fb
–1
, which almost doubled the total data collected since the collider operation began. The short description of the VEPP-2000 complex, current status, and the results are presented in this work.
A significant need for reliable and accurate cancer diagnostics and prognosis compels the search for novel biomarkers that would be able to discriminate between indolent and aggressive tumors at the ...early stages of disease. The aim of this work was identification of potential diagnostic biomarkers for characterization of different types of prostate tumors. NotI-microarrays with 180 clones associated with chromosome 3 genes/loci were applied to determine genetic and epigenetic alterations in 33 prostate tumors. For 88 clones, aberrations were detected in more than 10% of tumors. The major types of alterations were DNA methylation and/or deletions. Frequent methylation of the discovered loci was confirmed by bisulfite sequencing on selective sampling of genes: FGF12, GATA2, and LMCD1. Three genes (BHLHE40, BCL6, and ITGA9) were tested for expression level alterations using qPCR, and downregulation associated with hypermethylation was shown in the majority of tumors. Based on these data, we proposed the set of potential biomarkers for detection of prostate cancer and discrimination between prostate tumors with different malignancy and aggressiveness: BHLHE40, FOXP1, LOC285205, ITGA9, CTDSPL, FGF12, LOC440944/SETD5, VHL, CLCN2, OSBPL10/ZNF860, LMCD1, FAM19A4, CAND2, MAP4, KY, and LRRC58. Moreover, we probabilistically estimated putative functional relations between the genes within each set using the network enrichment analysis.
VEPP-2000 is the only electron–positron collider with round beams. This factor enables one to increase the beam–beam effect limit. During the 2018–2019 season, the experimental program was focused on ...the energy range of 550–990 MeV per beam. Data accumulation was performed using CMD-3 and SND detectors. The measured luminosity integral proved to have a record value of 68 pb
–1
per detector. After connecting to the BINP injection complex in 2016, the luminosity of VEPP-2000 is limited by the beam–beam effects in the entire energy range.
The cross section of the process e + e − → π + π − has been measured in the center of mass energy range from 0.32 to 1.2 GeV with the CMD-3 detector at the electron-positron collider VEPP-2000. The ...measurement is based on a full dataset collected below 1 GeV during three data taking seasons, corresponding to an integrated luminosity of about 62 pb − 1 . In the dominant ρ -resonance region, a systematic uncertainty of 0.7% has been reached. At energies around ϕ -resonance the π + π − production cross section was measured for the first time with high beam energy resolution. The forward-backward charge asymmetry in the π + π − production has also been measured. It shows a strong deviation from the theoretical prediction based on the conventional scalar quantum electrodynamics framework, and it is in good agreement with the generalized vector-meson-dominance and dispersive-based predictions. The impact of the presented results on the evaluation of the hadronic contribution to the anomalous magnetic moment of muon is discussed. Published by the American Physical Society 2024
Genetic and epigenetic alterations in cervical carcinomas were investigated using NotI-microarrays containing 180 cloned sequences flanking all NotI-sites associated with genes on chromosome 3. In ...total, 48 paired normal/tumor DNA samples, specifically enriched in NotI-sites, were hybridized to NotI-microarrays. Thirty genes, including tumor suppressors or candidates (for example, VHL, RBSP3/CTDSPL, ITGA9, LRRC3B, ALDH1L1, EPHB1) and genes previously unknown as cancer-associated (ABHD5, C3orf77, PRL32, LOC285375, FGD5 and others), showed methylation/deletion in 21-44% of tumors. The genes were more frequently altered in squamous cell carcinomas (SCC) than in adenocarcinomas (ADC, p < 0.01). A set of seven potential markers (LRRN1, PRICKLE2, VHL, BHLHE40, RBSP3, CGGBP1 and SOX14) is promising for discrimination of ADC and SCC. Alterations of more than 20 genes simultaneously were revealed in 23% of SCC. Bisulfite sequencing analysis confirmed methylation as a frequent event in SCC. High down-regulation frequency was shown for RBSP3, ITGA9, VILL, APRG1/C3orf35 and RASSF1 (isoform A) genes (3p21.3 locus) in SCC. Both frequency and extent of RASSF1A and RBSP3 mRNA level decrease were more pronounced in tumors with lymph node metastases compared with non-metastatic ones (p ≤ 0.05). We confirmed by bisulfite sequencing that RASSF1 promoter methylation was a rare event in SCC and, for the first time, demonstrated RASSF1A down-regulation at both the mRNA and protein levels without promoter methylation in tumors of this histological type. Thus, our data revealed novel tumor suppressor candidates located on chromosome 3 and a frequent loss of epigenetic stability of 3p21.3 locus in combination with down-regulation of genes in cervical cancer.
The cross section of the process
e
+
e
−
→ π
+
π
−
π
+
π
−
has been measured using an integrated luminosity of 17 pb
−1
collected with the CMD-3 detector in the center-of-mass energy range 650-1000 ...MeV. High-precision measurements of various hadronic cross sections are of great interest in relation with the problem of the muon anomalous magnetic moment g-2. This measurement can be also used to test the relation between the cross section of
e
+
e
−
→ π
+
π
−
π
+
π
−
and the spectral function for the τ
−
→ π
−
π
0
π
0
π
0
decay predicted by the conservation of vector current (CVC).
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