—
Spherical granules have been produced via suspension processing from synthetic ceramic powders consisting of calcium silicate hydrate and poorly crystallized carbonated hydroxyapatite in a wide ...range of ratios, using gelatin as a polymer binder. It has been shown that the experimental conditions of this study lead to the formation of polydisperse granules ranging in diameter from 500 μm to 5 mm and having open porosity on the order of 40–60%. The granulated materials contain 19–29 wt % gelatin and 60–75 wt % inorganic salts.
The exact cellular and molecular mechanisms of multiple sclerosis and other autoimmune diseases have not been established. Autoimmune pathologies are known to be associated with faults in the immune ...system and changes in the differentiation profiles of bone marrow stem cells. This study analyzed various characteristics of experimental autoimmune encephalomyelitis (EAE) in 2D2 mice. Differentiation profiles of six hematopoietic stem cells of bone marrow were found to significantly differ in 2D2 male and female mice during the spontaneous development of EAE. In addition, we found various properties of B and T cells, CD4+ and CD8+ lymphocytes in blood and several organs (bone marrow, spleen, thymus, and lymph nodes) of 2D2 male and female mice to be considerably different. These changes in hematopoietic stem cells differentiation profiles and level of lymphocyte proliferation in various organs of 2D2 mice were found to induce the production of IgGs against DNA, myelin basic protein, and myelin oligodendrocyte glycoprotein, increasing the number of autoantibodies hydrolyzing these substrates. We compared the changes of these immunological and biochemical parameters in 2D2 mice with those of mice of two other lines (Th and C57BL/6), also prone to spontaneous development of EAE. Some noticeable and even extreme variations were found in the time-related development of parameters between male and female mice of 2D2, Th, and C57BL/6 lines. Despite some differences, mice of all three lines demonstrated the changes in hematopoietic stem cells profiles, lymphocyte content, and production of catalytic autoantibodies. Given that these changes are harmful to mice, we believe them to cause the development of experimental autoimmune encephalomyelitis.
This article analyzes the magnetomotive forces (MFFs) created by fractional coil windings with number of coils per pole and phase
q
< 1 in slotless synchronous electric machines with permanent ...magnets. The harmonic composition of the MMF created by these windings is given. The influence of the coil width on the amplitude of the operating harmonics of the MMF is shown.
Vaccination protects against COVID-19 via the spike protein receptor-binding domain (RBD)-specific antibody formation, but it also affects the innate immunity. The effects of specific antibody ...induction on neutrophils that can cause severe respiratory inflammation are important, though not completely investigated. In the present study, using a mouse model mimicking SARS-CoV-2 virus particle inhalation, we investigated neutrophil phenotype and activity alterations in the presence of RBD-specific antibodies. Mice were immunized with RBD and a week after a strong antibody response establishment received 100 nm particles in the RBD solution. Control mice received injections of a phosphate buffer instead of RBD. We show that the application of 100 nm particles in the RBD solution elevates neutrophil recruitment to the blood and the airways of RBD-immunized mice rather than in control mice. Analysis of bone marrow cells of mice with induced RBD-specific antibodies revealed the increased population of CXCR2+CD101+ neutrophils. These neutrophils did not demonstrate an enhanced ability of neutrophil extracellular traps (NETs) formation compared to the neutrophils from control mice. Thus, the induction of RBD-specific antibodies stimulates the activation of mature neutrophils that react to RBD-coated particles without triggering excessive inflammation.
Display omitted
•X-band EPR setup at Novosibirsk Free Electron Laser facility is described.•Setup monitors the influence of high-power THz radiation on the spin system.•THz light induces the ...temperature change of the EPR sample.•T-jump transient EPR signals are observed.•Thermal dynamics of EPR samples is investigated.
Electron Paramagnetic Resonance (EPR) station at the Novosibirsk Free Electron Laser (NovoFEL) user facility is described. It is based on X-band (∼9 GHz) EPR spectrometer and operates in both Continuous Wave (CW) and Time-Resolved (TR) modes, each allowing detection of either direct or indirect influence of high-power NovoFEL light (THz and mid-IR) on the spin system under study. The optics components including two parabolic mirrors, shutters, optical chopper and multimodal waveguide allow the light of NovoFEL to be directly fed into the EPR resonator. Characteristics of the NovoFEL radiation, the transmission and polarization-retaining properties of the waveguide used in EPR experiments are presented. The types of proposed experiments accessible using this setup are sketched. In most practical cases the high-power radiation applied to the sample induces its rapid temperature increase (T-jump), which is best visible in TR mode. Although such influence is a by-product of THz radiation, this thermal effect is controllable and can deliberately be used to induce and measure transient signals of arbitrary samples. The advantage of tunable THz radiation is the absence of photo-induced processes in the sample and its high penetration ability, allowing fast heating of a large portion of virtually any sample and inducing intense transients. Such T-jump TR EPR spectroscopy with THz pulses has been previewed for the two test samples, being a useful supplement for the main goals of the created setup.
The discovery of oil deposits with complex internal structures requires an improvement of available seismic survey methods. The development of modern high-performance computing systems provides an ...opportunity to use more sophisticated mathematical models. This paper aims to investigate seismic wave propagation in porous fluid-saturated media. The Dorovsky three velocity model was formulated in the two-dimensional case and its numerical solution was obtained with the grid-characteristic method. The computational domain consisted of three layers with different rheology: a water layer, a porous fluid-saturated layer, and an elastic layer. Explicit contact conditions were derived between them and successfully applied with the help of Riemann invariants. The curvature of geological layers was taken into consideration by means of structured hexahedral grids. The time evolution of the spatial distribution of stress tensors and material velocity vectors were calculated and analyzed. These signals contain a mixture of volume, surface, transmitted and reflected waves.
Cells of various organs and systems perform their functions and intercellular interactions not in an inert environment, but in the microenvironment of tissue fluids. Violations of the normal drainage ...of tissue fluids accompany lymphedema. An important mechanism of angiogenesis and vasculogenesis regulation in tissue fluids is the production and reception of vascular endothelial growth factors in combination with the regulation of matrix metalloproteinases. The aim of the work was to perform: a comparative analysis of some polymorphisms of vascular endothelial growth factor and their receptors and the genes encoding matrix metalloproteinases in two forms of lymphedema; an analysis of the relationship of these genes’ polymorphisms with the levels of vascular endothelial growth factor and matrix metalloproteinases and their inhibitors in serum and affected tissues. Polymorphism of VEGF (rs699947, rs3025039), KDR (rs10020464, rs11133360), NRP2 (rs849530, rs849563, rs16837641), matrix metalloproteinases MMP2 (rs2438650), MMP3 (rs3025058), MMP9 (rs3918242), Timp1 (rs6609533) and their combinations were analyzed by the Restriction Fragment Length Polymorphism method and TaqMan RTPCR. The serum and tissue fluid levels were determined using the ELISA test system. Changes in the frequency distribution of MMP2 genotypes in primary and MMP3 in secondary lymphedema are shown. Significant frequency differences in NRP2 genotypes were revealed by comparing primary and secondary lymphedema. Features of the distribution of complex genotypes in primary and secondary lymphedema were revealed. The correlation analysis revealed the interdependence of the concentrations of the MMP, TIMP and VEGF products and differences in the structure of the correlation matrices of patients with both forms of lymphedema. It was shown that, in primary lymphedema, genotypes associated with low MMP2 and TIMP2 in serum and tissue fluid are detected, while in secondary lymphedema, other associations of the production levels with combined genetic traits are observed.
Osseointegrated implants often fail because of excessive masticatory forces that cause extremely large stresses in bone tissue. We hypothesized that a proper dental implant could be selected by ...comparing the ultimate masticatory forces of a wide range of commercially available implants. We determined ultimate oblique masticatory forces for different cylindrical implants, taking into consideration the biomechanical correlation between implant dimensions and stresses in supporting bone. For this purpose, we used the finite element (FE) method and studied von Mises stresses in implant-bone interface areas to evaluate the influence of implant dimensions on stress concentration and on the value of an implant’s ultimate masticatory load. Geometric models of a mandibular segment were generated from computed tomography (CT) images and were analyzed with osseointegrated cylindrical implants. Masticatory forces were applied in their natural direction. All materials were assumed to be linearly elastic and isotropic. Critical point in the peri-implant area of bone was determined. The ultimate value of the masticatory load, which generates ultimate stresses at the critical point, was calculated for each implant. These findings provide correct selection of implant dimensions in clinical cases, because corresponding ultimate values of masticatory force were used as a criterion for assessment of their load-carrying capacity and applicability.
Equalization enhanced phase noise (EEPN) occurs due to the interplay between laser phase noise and electronic dispersion compensation (EDC) module. It degrades significantly the performance of ...uncompensated long-haul coherent optical fiber communication systems. In this work, a general expression accounting for EEPN is presented based on Gaussian noise model to evaluate the performance of multi-channel optical communication systems using EDC and digital nonlinearity compensation (NLC). The nonlinear interaction between the signal and the EEPN is analyzed. Numerical simulations are carried out in nonlinear Nyquist-spaced wavelength division multiplexing (WDM) coherent transmission systems. Significant performance degradation due to EEPN in the cases of EDC and NLC are observed, with and without the consideration of transceiver (TRx) noise. The validation of the analytical approach has been done via split-step Fourier simulations. The maximum transmission distance and the laser linewidth tolerance are also estimated to provide important insights into the impact of EEPN.
In subwavelength-sized particles, light-induced multipole moments of orders higher than the electric dipole are usually negligibly small, which allows for the light-matter interaction to be ...accurately treated within the electric dipole approximation. In this work we show that in a specially designed meta-atom, a disc metadimer, the electric quadrupole and magnetic dipole can be the only excitable multipoles. This condition is achieved in a narrow but tunable spectral range of visible light both for individual metadimers and for a periodic array of such particles. The electromagnetic fields scattered by the metadimers fundamentally differ from those created by electric dipoles. A metamaterial composed of such metadimers will therefore exhibit unusual optical properties.
PDF
