To promote the energy-saving transformation of industrial enterprises effectively, it is important to accurately grasp the energy consumption behavior of enterprises. In this study, the multi-type ...industrial enterprises in an industrial park located in Shanghai are selected for analysis. Based on the collection and processing of the measured heating (steam) data, by employing the big data analysis method, the cluster analysis is carried out from different dimensions including user difference, load fluctuation and typical daily characteristics. Following which, the multi-type load characteristic curve is obtained, and the energy consumption characteristics of the whole industrial park and different types of industrial enterprises are discussed. On this basis, the energy load forecasting model of industrial enterprises is developed based on the LSTM neural network, and the effect of introducing meteorological data on the accuracy of load forecasting is analyzed.
Highlights
DOX-PLGA@CM employs the whole set of membrane molecules of a brain-homing metastatic breast cancer cell optimized through a natural selection process. Thus, the hetero and multivalent ...effects of these molecules greatly facilitate the nanoparticle crossing the blood-brain barrier.
Attributed to the homotypic effect of the nanocarrier, DOX-PLGA@CM shows stronger anticancer efficacy than free DOX for its parenteral cells.
DOX-PLGA@CM effectively reaches the metastatic tumor lesions in the brain, and slows down the progression of brain metastatic breast cancer.
Early-stage brain metastasis of breast cancer (BMBC), due to the existence of an intact blood–brain barrier (BBB), is one of the deadliest neurologic complications. To improve the efficacy of chemotherapy for BMBC, a Trojan horse strategy-based nanocarrier has been developed by integrating the cell membrane of a brain-homing cancer cell and a polymeric drug depot. With the camouflage of a MDA-MB-231/Br cell membrane, doxorubicin-loaded poly (D, L-lactic-co-glycolic acid) nanoparticle (DOX-PLGA@CM) shows enhanced cellular uptake and boosted killing potency for MDA-MB-231/Br cells. Furthermore, DOX-PLGA@CM is equipped with naturally selected molecules for BBB penetration, as evidenced by its boosted capacity in entering the brain of both healthy and early-stage BMBC mouse models. Consequently, DOX-PLGA@CM effectively reaches the metastatic tumor lesions in the brain, slows down cancer progression, reduces tumor burden, and extends the survival time for the BMBC animal. Furthermore, the simplicity and easy scale-up of the design opens a new window for the treatment of BMBC and other brain metastatic cancers.
Nb-doped Na0.7 Ni0.3Co0.1Mn0.60.98Nb0.02O2 as low-temperature cathodes for sodium ion batteries, demonstrates significantly enhanced long-term cycling ability with the efficient suppression of the ...voltage decay.
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The P2-type manganese-based Na0.7MnO2 cathode materials attract great interest due to their high theoretical capacity. However, these materials suffer from rapid capacity fading, poor rate performance and severe voltage decay resulting from phase transition and sluggish reaction kinetics. In this work we report a novel Nb-doped Na0.7Ni0.3Co0.1Mn0.61-xNbxO2 with significantly suppressed voltage decay and enhanced cycling stability. The strong Nb-O bond can efficiently stabilize the TMO framework, and the as prepared material demonstrates much lower discharge midpoint voltage decay (0.132 V) than that of pristine one (0.319 V) after 200 cycles. Consequently, a remarkably improved cycling performance with a capacity retention of 87.9% after 200 cycle at 0.5 C is achieved, showing a 2.4 fold improvement as compared to the control sample Na0.7Ni0.3Co0.1Mn0.6O2 (∼37% rotation). Even at 2 C, a capacity retention of 68.4% is retained after 500 cycles. Remarkably, the as prepared material can be applied at low temperature of −20 °C, showing a capacity retention of 81% as compared to that at room temperature.
Ischemic stroke (IS) is a common and grievous nervous system disease. Both autophagy activation and immune response after cerebral ischemia play important roles in the development of IS. Many studies ...have revealed a close interplay between autophagy and immunity. However, little is known about how autophagy influences the immune characteristics of IS. Hence, the study aims to systematically explore the role of autophagy and its impact on immune characteristics in IS. We first compared the expression differences of autophagy genes in a training set and identified 20 dysregulated autophagy genes between healthy and IS samples. An autophagy-related classifier composed of seven genes was further established and could well distinguish healthy and IS samples. Then, the association between autophagy and immune characteristics, including infiltrating immunocytes, activity of immune reactions, and HLA gene expression, was investigated. The results showed that autophagy closely correlated with immune characteristics, such as NAMPT and ARNT significantly related to infiltrating immunocytes; PPP1R15A and CASP3 significantly related to activity of immune reactions; and NAMPT and ATG16L2 significantly related to HLA genes. Next, two distinct autophagy expression patterns were identified by unsupervised clustering analysis, and diverse immune characteristics were discovered between them. A total of 5481 autophagy phenotype-related genes were obtained between two expression patterns, and their biological functions revealed that these genes were involved in immune-related biological pathways. Finally, five dysregulated autophagy genes (
,
,
,
, and
) were proved between IS and healthy samples using another two validation sets. Our results illustrated that autophagy had a dramatic effect on the immunity of IS and provided a novel sight into understanding the pathogenesis of IS.
Background:
Ischemic stroke (IS) is a common and serious neurological disease, and multiple pathways of cell apoptosis are implicated in its pathogenesis. Recently, extensive studies have indicated ...that pyroptosis is involved in various diseases, especially cerebrovascular diseases. However, the exact mechanism of interaction between pyroptosis and IS is scarcely understood. Thus, we aimed to investigate the impact of pyroptosis on IS-mediated systemic inflammation.
Methods:
First, the RNA regulation patterns mediated by 33 pyroptosis-related genes identified in 20 IS samples and 20 matched-control samples were systematically evaluated. Second, a series of bioinformatics algorithms were used to investigate the contribution of PRGs to IS pathogenesis. We determined three composition classifiers of PRGs which potentially distinguished healthy samples from IS samples according to the risk score using single-variable logistic regression, LASSO-Cox regression, and multivariable logistic regression analyses. Third, 20 IS patients were classified by unsupervised consistent cluster analysis in relation to pyroptosis. The association between pyroptosis and systemic inflammation characteristics was explored, which was inclusive of immune reaction gene sets, infiltrating immunocytes and human leukocyte antigen genes.
Results:
We identified that AIM2, SCAF11, and TNF can regulate immuno-inflammatory responses after strokes via the production of inflammatory factors and activation of the immune cells. Meanwhile, we identified distinct expression patterns mediated by pyroptosis and revealed their immune characteristics, differentially expressed genes, signaling pathways, and target drugs.
Conclusion:
Our findings lay a foundation for further research on pyroptosis and IS systemic inflammation, to improve IS prognosis and its responses to immunotherapy.
With the development of integrated energy system, energy flow analysis of coupling energy network has attracted much attention. In this study, based on the unified energy path theory, energy flow ...analysis of electricity-heating coupled energy system is implemented considering different time characteristic of each system. Considering the instantaneous change process of heat transfer in the heating system, the dynamic model of heat network is constructed based on unified energy path theory. On this basis, the dynamic models of electric and heating systems are coupled, and the dynamic energy flow calculation method of the coupling system is proposed. Through a case study, the feasibility of the proposed model and calculation method are verified. In addition, the effect of heat source on the dynamic process of heat transfer in the heating system is analyzed.
Numerous studies have confirmed the involvement of extracellular vesicles (EVs) in various physiological processes, including cellular death and tissue damage. Recently, we reported that EVs derived ...from ischemia-reperfusion heart exacerbate cardiac injury. However, the role of EVs from healthy heart tissue (heart-derived EVs, or cEVs) on myocardial ischemia-reperfusion (MI/R) injury remains unclear. Here, we demonstrated that intramyocardial administration of cEVs significantly enhanced cardiac function and reduced cardiac damage in murine MI/R injury models. cEVs treatment effectively inhibited ferroptosis and maintained mitochondrial homeostasis in cardiomyocytes subjected to ischemia-reperfusion injury. Further results revealed that cEVs can transfer ATP5a1 into cardiomyocytes, thereby suppressing mitochondrial ROS production, alleviating mitochondrial damage, and inhibiting cardiomyocyte ferroptosis. Knockdown of ATP5a1 abolished the protective effects of cEVs. Furthermore, we found that the majority of cEVs are derived from cardiomyocytes, and ATP5a1 in cEVs primarily originates from cardiomyocytes of the healthy murine heart. Moreover, we demonstrated that adipose-derived stem cells (ADSC)-derived EVs with ATP5a1 overexpression showed much better efficacy on the therapy of MI/R injury compared to control ADSC-derived EVs. These findings emphasized the protective role of cEVs in cardiac injury and highlighted the therapeutic potential of targeting ATP5a1 as an important approach for managing myocardial damage induced by MI/R injury.
The present study was designed to assess whether raised Serine protease inhibitor Kazal type 1 (SPINK1) expressions modulates angiogenesis. Human umbilical vein endothelial cells (HUVECs) exposed to ...SPINK1 were noted to exhibit raised expressions of interleukin-8 (IL-8) as well as VCAM-1 and ICAM-1 cell adhesion molecules in a dose-dependent manner. In co-culture system of HUVECs and Acute lymphoblastic leukemia (ALL) cells, SPINK1 exposure also resulted in enhanced endothelial cell motility and ALL cells trans-endothelial migration. High concentrations of SPINK1 caused in vitro cellular reorganization into tubes in Matrigel-cultured HUVECs and induced in vivo vascularization and brain infiltration of NOD/SCID ALL model mice. The further transcriptomic analysis indicated that SPINK1 treatment altered several biological processes of endothelial cells and led to activation of the MAPK pathway. This study is the first to determine the neovascularization effects of raised SPINK1.
The operation method of PA/IVS is selected in consideration of cardiac malformation and the developmental condition of right heart.1 2 The right heart of this child was severely hypoplastic, which ...could be regarded as functional single ventricle, so we decided to finally achieve a Fontan operation in this situation.3 4 Based on previous total cavopulmonary connection operation, an intra-extra cardiac Fontan operation was performed with fenestration between conduit and atrium to balance systemic pulmonary circulation, stabilizing the circulation and reducing hydrothorax and ascites. ...with lower half of conduit wrapped by atrium, anastomotic leakage would happen if the suture was not perfect or loosened. ...if cyanosis was found after intra-extra cardiac Fontan operation with fenestration, besides right to left shunt through fenestration due to increased pulmonary circulation pressure and resistance, a right to left shunt caused by anastomotic leakage should be considered as well. ...residual shunt at anastomosis between conduit and inferior vena cava is a rare cause of cyanosis in intra-extra cardiac Fontan operation. Ethics approval All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
Prolonged mechanical ventilation (PMV) after cardiac surgery in children is associated with a high postoperative morbidity and mortality, as well as increased ICU and hospital resource utilization. ...Little has been done to identify the predictors of PMV in neonates and young infants. This study was performed to evaluate the perioperative risk factors for PMV in neonates and young infants undergoing cardiac surgery.
Clinical records of 172 consecutive children aged ≤ 3 months were reviewed. PMV was defined as mechanical ventilation (MV) ≥ 72 h following operation. After univariate analysis, a stepwise logistic regression analysis was used to evaluate the independent risk factors for PMV following cardiac surgery. The predictive ability of risk factors for PMV was assessed using an area under the receiver operating characteristic (ROC) curve.
Sixty-one patients required PMV after cardiac surgery. The median duration of MV was 150 h in PMV patients, while it was 28 h in non-PMV patients. The independent risk factors for PMV were risk adjustment for surgery for congenital heart disease (RACHS)-1 (p = 0.041), nosocomial pneumonia (p = 0.001), low cardiac output syndrome (LCOS) p = 0.001, postoperative cumulative positive fluid balance (p = 0.032), and extubation failure (EF) p = 0.027. The value for the ROC curve was 0.940.
The present results strongly suggest that RACHS-1, nosocomial pneumonia, LCOS, fluid retention postoperatively, and EF are risk factors for PMV in neonates and young infants undergoing reparative surgery for congenital heart disease.
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