Atopic dermatitis (AD) is a common relapsing inflammatory skin disorder characterized by immune-mediated inflammation and epidermal barrier dysfunction. The pathogenesis of AD is multifactorial and ...has not been fully elucidated to date. This study aimed to evaluate whether serum IgG from adult AD patients could modulate the thymic maturation of IL-22-producing T cells and CLA+ T cells of non-atopic infants. Given that miRNAs regulate immune response genes, we evaluated whether miRNA expression is also altered in cultured thymocytes. Thymocytes were cultured with purified IgG from AD patients or control conditions (mock, Intravenous-IgG (IVIg), non-atopic IgG, or atopic non-AD IgG). Using flow cytometry analysis, we assessed the expression of CLA and intracellular levels of IL-4, IFN-γ, and IL-22 on double-positive T cells (DP T), CD4 T cells, or CD8 T cells. We also investigated the frequency of IgG isotypes and their direct interaction with the thymic T cells membrane. The miRNA profiles were evaluated by the Illumina small RNA-seq approach. MiRNA target gene prediction and enrichment analyses were performed using bioinformatics. Increased frequencies of IL-22 and CLA+ producing CD4+ T cells cultured with IgG of AD patients was seen in non-atopic infant thymocytes compared to all control conditions. No alterations were observed in the frequency of IgG isotypes among evaluated IgG pools. Evidence for a direct interaction between IgG and thymic DP T, CD4 T, and CD8 T cells is presented. The small RNA-seq analysis identified ten mature miRNAs that were modulated by AD IgG compared to mock condition (miR-181b-5p, hsa-miR-130b-3p, hsa-miR-26a-5p, hsa-miR-4497, has-miR-146a, hsa-let-7i-5p, hsa-miR-342-3p, has-miR-148a-3p, has-miR-92a and has-miR-4492). The prediction of the targetome of the seven dysregulated miRNAs between AD and mock control revealed 122 putative targets, and functional and pathway enrichment analyses were performed. Our results enhance our understanding of the mechanism by which IgG can collaborate in thymic T cells in the setting of infant AD.
The Brazil nut tree is considered symbolic of the Brazilian Amazon in function of its great importance, being one of the most significant extractivist products and a subsistence practice of the ...Indigenous people in many municipalities in Pará state. One of the main problems in different communities is related to the marketing process since it is not possible to distinguish the origin of the nuts and this causes inconvenience. The study evaluated the potential of VIS/NIR spectroscopy to identify the origin of leaves and nuts from Brazil nut trees growing in two indigenous villages, in the Xipaya Indigenous Lands, Pará state. Analysis was performed based on CIEL*a*b* parameters and using VIS (360–740 nm) and near-infrared spectra (1000–2500 nm). The samples were differentiated according to means tests, principal component analysis (PCA), and classification analysis based on k-NN. Color parameters and spectra were similar in both communities. Classification models based on k-NN produced adequate results for the distinction of villages in all evaluated situations, with accuracy of 98.54% for leaves, 89% and 90.91% for nuts with and without shell, respectively. Near infrared can be applied in forests as a technique for previous provenance identification and contribute to the subsistence and sustainable practice of extraction.
Small RNAs (sRNAs) are epigenetic regulators of essential biological processes associated with the development and progression of leukemias, including adult T-cell leukemia/lymphoma (ATLL) caused by ...human T-cell lymphotropic virus type 1 (HTLV-1), an oncogenic human retrovirus originally discovered in a patient with adult T-cell leukemia/lymphoma. Here, we describe the sRNA profile of a 30-year-old woman with ATLL at the time of diagnosis and after maintenance therapy with the aim of correlating expression levels with response to therapy.
γδT cells mature in the human thymus, and mainly produce IL-17A or IFN-γ, but can also produce IL-22 and modulate a variety of immune responses. Here, we aimed to evaluate whether IgG from AD ...patients (AD IgG) can functionally modulate thymic nonatopic γδT cells. Thymic tissues were obtained from 12 infants who had not had an atopic history. Thymocytes were cultured in mock condition, or in the presence of either AD IgG or therapeutic intravenous IgG (IVIg). Following these treatments, intracellular cytokine production, phenotype, and microRNA expression profiles were investigated. AD IgG could downregulate α4β7, upregulate CLA, and induce the production of IFN-γ, IL-17, and IL-22 in γδT cells. Although both AD IgG and IVIg could directly interact with γδT cell membranes, AD IgG could reduce γδT cell apoptosis. AD IgG could upregulate nine miRNAs compared to IVIg, and six when compared to the mock condition. In parallel, some miRNAs were downregulated. Target gene prediction and functional analysis indicated that some target genes were enriched in the negative regulation of cellular transcription. This study shows that AD IgG influences the production of IL-17 and IL-22 by intrathymic nonatopic γδT cells, and demonstrates epigenetic implications mediated by miRNAs.
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The present study evaluated the role of heme oxygenase 1 (HO-1)/carbon monoxide (CO) pathway in the cholera toxin-induced diarrhea and its possible action mechanism. The ...pharmacological modulation with CORM-2 (a CO donor) or Hemin (a HO-1 inducer) decreased the intestinal fluid secretion and Cl− efflux, altered by cholera toxin. In contrast, ZnPP (a HO-1 inhibitor) reversed the antisecretory effect of Hemin and potentiated cholera toxin-induced intestinal secretion. Moreover, CORM-2 also prevented the alteration of intestinal epithelial architecture and local vascular permeability promoted by cholera toxin. The intestinal absorption was not altered by any of the pharmacological modulators. Cholera toxin inoculation also increased HO-1 immunoreactivity and bilirubin levels, a possible protective physiological response. Finally, using fluorometric technique, ELISA assay and molecular docking simulations, we show evidence that CO directly interacts with cholera toxin, forming a complex that affects its binding to GM1 receptor, which help explain the antisecretory effect. Thus, CO is an essential molecule for protection against choleric diarrhea and suggests its use as a possible therapeutic tool.
In the present study, the gastroprotective effect of a sulfated polysaccharide isolated from marine alga Agardhiella ramosissima (SP-Ar) was investigated against alcoholic gastropathy. Gastric ...physiological functions and in vitro antioxidant activity also were studied. It was found that SP-Ar significantly reduced macroscopic injury and histologically maintained the architecture of gastric epithelium altered by ethanol, with maximum effect at 90 mg/kg. Importantly, animals treated with SP-Ar also had preservation of oxidative status, with reduction of nonprotein sulfhydryl groups (NP-SH) consumption and malondialdehyde (MDA) production, reduced vascular permeability, besides preventing gastric hemorrhage. In addition, SP-Ar administration did not modify gastric secretion or emptying. In vitro antioxidant data showed that SP-Ar presented high total antioxidant activity, DPPH radical scavenging, and ferrous iron chelating capacity. It was observed that SP-Ar protected gastric mucosa against alcoholic gastropathy by preventing the formation or neutralizing free radicals, associated with its intrinsic antioxidant activity. In this regard, this macromolecule should have possible application as a new therapeutic alternative or food additive to increase gastric mucosa defense.
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•Sulfated iota-carrageenan from Agardhiella ramosissima (SP-Ar) reduces ethanol-induced gastric injury.•SP-Ar preserves the balance in the oxidative status in the ethanol-induced gastric injury.•SP-Ar does not modify gastric secretion or emptying.•SP-Ar has in vitro antioxidant activity.
O câncer de colo de útero é o tipo de câncer que mais mata mulheres no mundo. Este estudo teve por objetivo, analisar as evidências científicas da educação em saúde como forma de prevenção do câncer ...de colo de útero. Trata-se de uma revisão integrativa da literatura, realizada através das bases de dados da BVS e da PUBMED, na qual se utilizou a seguinte questão norteadora: “Quais as evidências científicas apontam para educação em saúde como forma de prevenção do câncer de colo de útero?”. Utilizaram-se estudos disponíveis na íntegra, nos idiomas português, inglês e espanhol, publicados de 2017 a 2022. Foram selecionados 10 estudos para compor esta revisão. Conforme os estudos analisados, foi identificado que os fatores desencadeantes do câncer de colo do útero são, início da vida sexual precoce, relação sexual desprotegida, múltiplos parceiros sexuais, infecções pelo HPV, e uso de contraceptivos hormonais. As atividades de educação em saúde como palestras, rodas de conversas, visitas domiciliares se mostram relevantes, pois levam conhecimento para as mulheres acerca da neoplasia, incentivando a prática do autocuidado, bem como auxilia na desconstrução de estigmas e receios relacionados ao exame citopatológico. Evidenciou-se que a escassez de informações sobre o CCU e as formas de prevenção da doença provocam medos e receios para muitas mulheres, no qual contribui para um retardo na realização do exame citopatológico. Assim, estratégias de educação em saúde, com esclarecimento de dúvidas sobre a doença e as formas de prevenção, abordando a disponibilidade dos serviços de saúde para realização do rastreamento precoce, são medidas imprescindíveis para quebrar o tabu associado à realização do exame.
The Brazil nut tree is considered symbolic of the Brazilian Amazon in function of its great importance, being one of the most significant extractivist products and a subsistence practice of the ...Indigenous people in many municipalities in Pará state. One of the main problems in different communities is related to the marketing process since it is not possible to distinguish the origin of the nuts and this causes inconvenience. The study evaluated the potential of VIS/NIR spectroscopy to identify the origin of leaves and nuts from Brazil nut trees growing in two indigenous villages, in the Xipaya Indigenous Lands, Pará state. Analysis was performed based on CIEL*a*b* parameters and using VIS (360–740 nm) and near-infrared spectra (1000–2500 nm). The samples were differentiated according to means tests, principal component analysis (PCA), and classification analysis based on k-NN. Color parameters and spectra were similar in both communities. Classification models based on k-NN produced adequate results for the distinction of villages in all evaluated situations, with accuracy of 98.54% for leaves, 89% and 90.91% for nuts with and without shell, respectively. Near infrared can be applied in forests as a technique for previous provenance identification and contribute to the subsistence and sustainable practice of extraction.
AUTOMEDICAÇÃO EM IDOSOS: MEDIDAS DE PREVENÇÃO E CONTROLE Pereira Rocha Beserra, Fernanda Lorena; Borba, Valéria Ferreira da Costa; Torres, Jáfia Elana Gonçalves ...
Revista Contexto & Saúde,
12/2019, Letnik:
19, Številka:
37
Journal Article
Odprti dostop
RESUMO
Os medicamentos são considerados essenciais para os cuidados com a saúde, os quais colaboram para se obter uma melhora significativa no estado de saúde das pessoas, nos casos em que o ...utilizam de modo racional. Os idosos são os que mais consomem medicamentos sem prescrição. Diante desse cenário, o profissional farmacêutico é visto como sendo um profissional de saúde de fácil acesso, o qual é encontrado em quase todas as farmácias do país. O objetivo desse estudo foi analisar a prevenção e o controle da automedicação no público idoso e a identificação das medidas de prevenção e controle da automedicação. Trata-se de uma Revisão Integrativa da Literatura realizada na Biblioteca Virtual de Saúde (BVS), onde foram usados os descritores indexados nos Descritores em Ciências da Saúde (DECS) “Automedicação” e “Idoso”. A busca foi realizada no período de fevereiro e março de 2018, e foram encontradas doze publicações na pesquisa, onde após os critérios de inclusão restaram nove trabalhos relacionados à temática. Os resultados mostraram que. As principais medidas identificadas de controle e prevenção da automedicação em idosos foram à atuação da equipe de saúde, dando destaque ao farmacêutico, na divulgação de informações e esclarecimentos quanto aos riscos da automedicação através da adoção de práticas educativas e o uso de recurso midiático para divulgação, protocolos clínicos e listas de medicações e suas especificidades. Contudo, a limitação do estudo deu-se por um quantitativo reduzido de produções, assim, sugerem-se estudos mais amplos que abordem essa temática, incluindo outros públicos e outros contextos.
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