Background. Stroke-related epilepsy (STRE) represents a significant health problem. We focused on identifying brain areas, which involvement in ischemia predisposes a patient to STRE development. ...Methods. We retrospectively identified a group of patients with STRE consisting of 33 subjects. Subsequently, age-, sex-, and territory-matched controls who underwent stroke but did not develop STRE (control group (CG)) were identified. The CG was composed of 37 patients. The total ischemia volume and distribution of ischemic changes were compared between STRE and CG. We also analyzed multivariate statistics to identify independent variables predisposing to STRE development. Results. The patients with STRE exhibited a bigger volume of ischemia than CG (average volume of ischemia in STRE 60.8 cm3, in CG 42.4 cm3, p=0.029). When comparing STRE and CG, there were differences in the distribution of ischemic changes in the temporal lobe (transverse (Heschl’s) temporal gyri, superior temporal gyrus, and middle temporal gyrus) and parietooccipital region (postcentral gyrus, supramarginal gyrus, angular gyrus, parietal operculum, lingual gyrus, and superior occipital gyrus). The involvement of transverse temporal (Heschl’s) gyri (p=0.0222, odds ratio 30.0767), age (p=0.0110, odds ratio 1.0745), and SeLECT score (p=0.0480, odds ratio 1.8682) were identified as independent predictors for STRE development. Conclusion. The higher volume of ischemia correlates with a higher risk of STRE development. Some areas, particularly in the temporal and parietal neocortex, predispose the brain to generate epilepsy after the stroke.
Celotno besedilo
Dostopno za:
BFBNIB, DOBA, FZAB, GIS, IJS, KILJ, NUK, OILJ, SBCE, SBMB, SIK, UILJ, UKNU, UL, UM, UPUK
We identified transcriptomic heterogeneity within Gleason pattern 4 subtypes. Our findings demonstrate there is additional biological diversity not fully captured by histologic subtypes. This ...heterogeneity can be used to develop novel signatures and transcriptomic subtypes, which may help in further refining risk stratification following radical prostatectomy.
Prostate cancers featuring an expansile cribriform (EC) pattern are associated with worse clinical outcomes following radical prostatectomy (RP). However, studies of the genomic characteristics of Gleason pattern 4 subtypes are limited.
To explore transcriptomic characteristics and heterogeneity within Gleason pattern 4 subtypes (fused/poorly formed, glomeruloid, small cribriform, EC/intraductal carcinoma IDC) and the association with biochemical recurrence (BCR)-free survival.
This was a retrospective cohort study including 165 men with grade group 2–4 prostate cancer who underwent RP at a single academic institution (2016–2020) and Decipher testing of the RP specimen. Patients with Gleason pattern 5 were excluded. IDC and EC patterns were grouped. Median follow-up was 2.5 yr after RP for patients without BCR.
Prompted by heterogeneity within pattern 4 subtypes identified via exploratory analyses, we investigated transcriptomic consensus clusters using partitioning around medoids and hallmark gene set scores. The primary clinical outcome was BCR, defined as two consecutive prostate-specific antigen measurements >0.2 ng/ml at least 8 wk after RP, or any additional treatment. Multivariable Cox proportional-hazards models were used to determine factors associated with BCR-free survival.
In this cohort, 99/165 patients (60%) had EC and 67 experienced BCR. Exploratory analyses and clustering demonstrated transcriptomic heterogeneity within each Gleason pattern 4 subtype. In the multivariable model controlled for pattern 4 subtype, margin status, Cancer of the Prostate Risk Assessment Post-Surgical score, and Decipher score, a newly identified steroid hormone–driven cluster (hazard ratio 2.35 95% confidence interval 1.01–5.47) was associated with worse BCR-free survival. The study is limited by intermediate follow-up, no validation cohort, and lack of accounting for intratumoral and intraprostatic heterogeneity.
Transcriptomic heterogeneity was present within and across each Gleason pattern 4 subtype, demonstrating there is additional biologic diversity not captured by histologic subtypes. This heterogeneity can be used to develop novel signatures and to classify transcriptomic subtypes, which may help in refining risk stratification following RP to further guide decision-making on adjuvant and salvage treatments.
We studied prostatectomy specimens and found that tumors with similar microscopic appearance can have genetic differences that may help to predict outcomes after prostatectomy for prostate cancer. Our results demonstrate that further gene expression analysis of prostate cancer subtypes may improve risk stratification after prostatectomy. Future studies are needed to develop novel gene expression signatures and validate these findings in independent sets of patients.
Precise investigation of part of the phase diagram of KF–Al2O3 system was performed in an experiment combining different techniques. Solidified mixtures of KF–Al2O3 were studied by X-ray powder ...diffraction and high-field solid-state NMR spectroscopy over a wide range of compositions. To help with the interpretation of the NMR spectra of the solidified samples found as complex admixtures, we synthesized the following pure compounds: KAlO2, K2Al22O34, α-K3AlF6, KAlF4, and K2Al2O3F2. These compounds were then characterized using various solid-state NMR techniques, including MQ-MAS and D-HMQC. NMR parameters of the pure compounds were finally determined using first-principles density functional theory calculations. The phase diagram of KF–Al2O3 with the alumina content up to 30 mol % was determined by means of thermal analysis. Thermal analysis was also used for the description of the thermal stability of one synthesized compound, K2Al2O3F2.
The effects of aluminium phosphate dissolution on speciation in Na
3
AlF
6
-Al
2
O
3
melts was studied. The structural description was performed
via
multinuclear (
17
O,
19
F,
23
Na,
27
Al, and
31
P) ...NMR measurements
in situ
at high temperature (HT) in melts of the Na
3
AlF
6
-Al
2
O
3
-AlPO
4
system. Consistent with our previous findings, the PO
4
3−
, AlF
5
2−
, Al
2
OF
6
2−
, Al
2
O
2
F
4
2−
, and (AlF
4
-O-PO
3
)
4−
anions are present in the melt. When AlPO
4
is introduced in the melt containing alumina, the formation of Al-O-P linkages become more favorable compared with the system without alumina.
The formation of complex oxofluoroaluminate species containing bridging oxygen atoms from the chemical processes between AlPO
4
, Al
2
O
3
, and Na
3
AlF
6
.
Abstract The article presents a theoretical analysis of electromagnetic compatibility (EMC) and experimental measurement of effects of radiation and acoustic emission of high-voltage transformers for ...electronic equipment and working personnel in a control room. Electromagnetic compatibility and safety of equipment are not considered as two distinct areas of study in electric and electronic safety. Economic criteria cannot compromise safety but at the same time immunity levels must be relevant in order to establish a “Functional Safety”. Introducing Special Immunity Levels in the level of equipment testing allows us to combine the two areas of EMC and safety. The measurement was carried out in high-current of very high-voltage distribution station. A real-life analysis of effects of electric and electromagnetic field was carried out. FFT was used for mathematical processing of data which were later presented in a graphical form of a spectrally analyzed area. In the last part of the paper we discuss the suitability of acoustic camera to perform contactless monitoring of the health and operation conditions of the power transformer by analyzing acoustic field generated by the transformer core and windings in near control room.
Precise research on the RbF–Al2O3 system was carried out by means of combining X-ray powder diffraction, high-field solid-state NMR spectroscopy, and thermal analysis methods. α-Rb3AlF6, RbAlO2, ...Rb2Al22O34, and new phase, Rb2Al2O3F2, were identified in the system. The structure of this new rubidium oxofluoroaluminate was determined. It is built up from single layers of oxygen-connected AlO3F tetrahedra, those layers beeing separated by fluorine atoms. This type of structure exhibits a decent ionic conductivity at ambient temperature, 1.74 × 10–6 S cm–1. The similar structural arrangement of O3Al–O–AlO3 and FO2Al–O–AlO2F tetrahedra of the conduction planes in Rb2Al22O34 and Rb2Al2O3F2 were confirmed by 27Al NMR measurements. A thermal analysis of the RbF–Al2O3 system revealed that it can be defined as a pseudobinary subsystem of the more general quaternary RbF–AlF3–Al2O3–Rb2O phase diagram. From a phase analysis of individual phase fields, the mutual metastable behavior of all founded phases can be considered. It was observed that fluoro- and oxoaluminates exist together. Rb2Al2O3F2 is more stable under high temperature. Rubidium fluoro- and oxoaluminates are metastable precursors of the thermodynamically more stable structure of rubidium oxofluoroaluminate.
PTEN loss is a promising prognostic and predictive biomarker in prostate cancer. Because it occurs most commonly via PTEN gene deletion, we developed a clinical-grade, automated, and inexpensive ...immunohistochemical assay to detect PTEN loss. We studied the sensitivity and specificity of PTEN immunohistochemistry relative to four-color fluorescence in situ hybridization (FISH) for detection of PTEN gene deletion in a multi-institutional cohort of 731 primary prostate tumors. Intact PTEN immunostaining was 91% specific for the absence of PTEN gene deletion (549/602 tumors with two copies of the PTEN gene by FISH showed intact expression of PTEN by immunohistochemistry) and 97% sensitive for the presence of homozygous PTEN gene deletion (absent PTEN protein expression by immunohistochemistry in 65/67 tumors with homozygous deletion). PTEN immunohistochemistry was 65% sensitive for the presence of hemizygous PTEN gene deletion, with protein loss in 40/62 hemizygous tumors. We reviewed the 53 cases where immunohistochemistry showed PTEN protein loss and FISH showed two intact copies of the PTEN gene. On re-review, there was ambiguous immunohistochemistry loss in 6% (3/53) and failure to analyze the same tumor area by both methods in 34% (18/53). Of the remaining discordant cases, 41% (13/32) revealed hemizygous (n=8) or homozygous (n=5) PTEN gene deletion that was focal in most cases (11/13). The remaining 19 cases had two copies of the PTEN gene detected by FISH, representing truly discordant cases. Our automated PTEN immunohistochemistry assay is a sensitive method for detection of homozygous PTEN gene deletions. Immunohistochemistry screening is particularly useful to identify cases with heterogeneous PTEN gene deletion in a subset of tumor glands. Mutations, small insertions, or deletions and/or epigenetic or microRNA-mediated mechanisms may lead to PTEN protein loss in tumors with normal or hemizygous PTEN gene copy number.
Age-related changes in EEG coherence Vysata, Oldrich; Kukal, Jaromir; Prochazka, Ales ...
Neurologia i Neurochirurgia Polska,
January-February 2014, 2014 Jan-Feb, 2014-01-00, 20140101, Letnik:
48, Številka:
1
Journal Article
Recenzirano
Odprti dostop
Coherence changes can reflect the pathophysiological processes involved in human ageing. We conducted a retrospective population study that sought to analyze the age-related changes in EEG coherence ...in a group of 17,722 healthy professional drivers.
The EEGs were obtained using a standard 10–20 electrode configuration on the scalp. The recordings from 19 scalp electrodes were taken while the participants’ eyes were closed. The linear correlations between the age and coherence were estimated by linear regression analysis.
Our results showed a significant decrease in coherence with age in the theta and alpha bands, and there was an increasing coherence with the beta bands. The most prominent changes occurred in the alpha bands. The delta bands contained movement artefacts, which most likely do not change with age.
The age-related EEG desynchrony can be partly explained by the age-related reduction of cortical connectivity. Higher frequencies of oscillations require less cortical area of high coherence. These findings explain why the lowest average coherence values were observed in the beta and sigma bands, as well as why the beta bands show borderline statistical significance and the sigma bands show non-significance. The age-dependent decrease in coherence may influence the estimation of age-related changes in EEG energy due to phase cancellation.
Abstract Elevated cholesterol intake can induce the development of cardiovascular diseases in man, especially with long term animal origin foods consumption. Therefore, this work deals with the ...possibility of cholesterol content decrease in milk applying β-cyclodextrin crosslinked with tartaric acid (βCDcTA) as a removal agent. Evaluation of statistic data on food consumption in the Slovak Republic in 2018 aimed at total cholesterol daily intake and effects of “milky” cholesterol content decrease on total cholesterol balance. During the experiments, various amounts of βCDcTA addition to milk were studied resulting in optimal 5 % addition resulting in the cholesterol content decrease by 85.4 % in comparison to original cholesterol content. For monitoring purposes, an HPLC method analysing cholesterol content in saponified milk was employed. The food consumption data analysis showed that total per capita daily cholesterol intake was 369.8 mg, from which 86 mg was assigned to the cholesterol contained in milk and dairy products while the application of cholesterol removal procedure could decrease the total per capita daily cholesterol intake to 296.3 mg (“milky” cholesterol amount equal to 12.6 mg), which in below the recommended value of 300 mg daily intake still valid in the Slovak Republic. This approach might prove as a meaningful step to weaken health problems associated with high long term intake of cholesterol contained in foods of animal origin.
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