We studied the application of statistical reconstruction algorithms, namely maximum likelihood and least squares methods, to the problem of event reconstruction in a dual phase liquid xenon detector. ...An iterative method was developed for in-situ reconstruction of the PMT light response functions from calibration data taken with an uncollimated γ -ray source. Using the techniques described, the performance of the ZEPLIN-III dark matter detector was studied for 122 keV γ-rays. For the inner part of the detector ( R <; 100 mm) , spatial resolutions of 13 mm and 1.6 mm FWHM were measured in the horizontal plane for primary and secondary scintillation, respectively. An energy resolution of 8.1% FWHM was achieved at that energy. The possibility of using this technique for improving performance and reducing cost of scintillation cameras for medical applications is currently under study.
We present the detection of very-high-energy gamma-ray emission above 100 TeV from HAWC J2227+610 with the High-Altitude Water Cherenov Gamma-Ray Observatory (HAWC) observatory. Combining our ...observations with previously published results by the Very Energetic Radiation Imaging Telescope Array System (VERTIAS), we interpret the gamma-ray emission from HAWC J2227+610 as emission from protons with a lower limit in their cutoff energy of 800 TeV. The most likely source of the protons is the associated supernova remnant G106.3+2.7, making it a good candidate for a Galactic PeVatron. However, a purely leptonic origin of the observed emission cannot be excluded at this time.
Summary
Background
Pannexin‐1 (Panx1) forms an anion‐selective channel with a permeability up to ~1 kDa and represents a non‐lytic, non‐vesicular ATP release pathway in erythrocytes, leukocytes and ...neurons. Related connexin gap junction proteins have been reported in platelets; however, the expression and function of the pannexins remain unknown.
Objective
To determine the expression and function of pannexins in human plate‐lets, using molecular, cellular and functional techniques.
Methods
Panx1 expression in human platelets was det‐ermined using qPCR and antibody‐based techniques. Contributions of Panx1 to agonist‐evoked efflux of cytoplasmic calcein, Ca2+ influx, ATP release and aggregation were assessed in washed platelets under conditions where the P2X1 receptor response was preserved (0.32 U mL−1 apyrase). Thrombus formation in whole blood was assessed in vitro using a shear chamber assay. Two structurally unrelated and widely used Panx1 inhibitors, probenecid and carbenoxolone, were used throughout this study, at concentrations that do not affect connexin channels.
Results
PANX1, but not PANX2 or PANX3, mRNA was detected in human platelets. Furthermore, Panx1 protein is glycosylated and present on the plasma membrane of platelets, and displays weak physical association with P2X1 receptors. Panx1 inhibition blocked thrombin‐evoked efflux of calcein, and reduced Ca2+ influx, ATP release, platelet aggregation and thrombus formation under arterial shear rates in vitro. The Panx1‐dependent contribution was not additive to that of P2X1 receptors.
Conclusions
Panx1 is expressed on human platelets and amplifies Ca2+ influx, ATP release and aggregation through the secondary activation of P2X1 receptors. We propose that Panx1 represents a novel target for the management of arterial thrombosis.
We present first results for Faraday rotation of compact polarized sources (1-2 GHz continuum) in The H i/OH/Recombination line (THOR) survey of the inner Galaxy. In the Galactic longitude range 39° ...< < 52°, we find rotation measures (RMs) in the range −310 rad m−2 ≤ RM ≤ +4219 rad m−2, with the highest values concentrated within a degree of = 48° at the Sagittarius arm tangent. Most of the high RMs arise in diffuse plasma, along lines of sight that do not intersect H ii regions. For > 49°, RM drops off rapidly, while at < 47°, the mean RM is higher with a larger standard deviation than at > 49°. We attribute the RM structure to the compressed diffuse warm ionized medium in the spiral arm, upstream of the major star formation regions. The Sagittarius arm acts as a significant Faraday screen inside the Galaxy. This has implications for models of the Galactic magnetic field and the expected amount of Faraday rotation of fast radio bursts from their host galaxies. We emphasize the importance of sensitivity to high Faraday depth in future polarization surveys.
The mechanism and kinetics of interactions between dimethyl methylphosphonate (DMMP), a key chemical warfare agent (CWA) simulant, and Zr6-based metal organic frameworks (MOFs) have been investigated ...with in situ infrared spectroscopy (IR), X-ray photoelectron spectroscopy (XPS), powder X-ray diffraction (PXRD), and DFT calculations. DMMP was found to adsorb molecularly to UiO-66 through the formation of hydrogen bonds between the phosphoryl oxygen and the free hydroxyl groups associated with Zr6 nodes on the surface of crystallites and not within the bulk MOF structure. Unlike UiO-66, the infrared spectra for UiO-67 and MOF-808, recorded during DMMP exposure, suggest that uptake occurs through both physisorption and chemisorption. The XPS spectra of MOF-808 zirconium 3d electrons reveal a charge redistribution following exposure to DMMP. In addition, analysis of the phosphorus 2p electrons following exposure and thermal annealing to 600 K indicates that two types of stable phosphorus-containing species exist within the MOF. DFT calculations, used to guide the IR band assignments and to help interpret the XPS features, suggest that uptake is driven by nucleophilic addition of an OH group to DMMP with subsequent elimination of a methoxy substituent to form strongly bound methyl methylphosphonic acid (MMPA). The rates of product formation indicate that there are likely two distinct uptake processes, requiring rate constants that differ by approximately an order of magnitude. However, the rates of molecular uptake were found to be nearly identical to the rates of reaction, which strongly suggests that the reaction rates are diffusion-limited. The final products were found to inhibit further reactions within the MOFs, and these products could not be thermally driven from the MOFs prior to decomposition of the MOFs themselves.
On 23 October 2015 at ~1732 UTC, the Airborne Detector for Energetic Lightning Emissions (ADELE) flew through the eyewall of Hurricane Patricia aboard National Oceanic and Atmospheric ...Administration's Hurricane Hunter WP‐3D Orion, observing the first terrestrial gamma‐ray flash (TGF) ever seen in that context, and the first ever viewed from behind the forward direction of the main TGF gamma‐ray burst. ADELE measured 184 counts of ionizing radiation within 150 μs, coincident with the detection of a nearby lightning flash. Lightning characteristics inferred from the associated radio signal and comparison of the gamma‐ray energy spectrum to simulations suggests that this is the first observation of a reverse beam of positrons predicted by the leading TGF production model, relativistic runaway electron avalanches. This paper presents the first experimental evidence of a previously predicted second component of gamma‐ray emission from TGFs. The brightest emission, commonly observed from orbit, is from the relativistic runaway electron avalanche bremsstrahlung; the second, fainter component reported here is from the bremsstrahlung of positrons propagating in the reverse direction. This reverse gamma‐ray beam penetrates to low enough altitudes to allow ground‐based detection of typical upward TGFs from mountain observatories.
Plain Language Summary
We report the first observation of gamma‐ray emission from lightning within a hurricane eyewall, consistent with production by a downward beam of positrons.
Key Points
We report the first in situ observation of a terrestrial gamma‐ray flash inside a hurricane eyewall
Observed gamma‐ray spectra, lightning data, and meteorological scenario are consistent with production of a downward beam of positrons
Simulations of the downward positron beam establish ordinary TGFs as detectable at almost any altitude
The purpose of this study was to evaluate food insecurity on body mass index (BMI) and diet-related behaviors among college students and whether psychological well-being (PWB) and stress levels ...mediate this relationship.
This was a cross-sectional study.
Data from 1439 students from the American College Health Association National College Health Assessment III (Fall 2020) were used. Food security status was evaluated by the USDA Six-Item Short Form. PWB was measured using the Diener Flourishing Scale. Diet-related behaviors included the average servings of fruits, vegetables, and sugar-sweetened beverages consumed per day. Stress was measured by self-reported levels. Regression model analysis evaluated the influence of food security status, PWB, and stress levels on BMI. PWB and stress were also tested as mediators in the relationship between food insecurity and BMI.
Among our sample of college students, 44.54% (n = 641) were food insecure, and 55.46% (n = 798) were food secure. Multiple regression analysis showed that higher food insecurity, older age, full-time enrollment status, and fifth-year student status were positively associated with a higher BMI score (P < 0.05). Results from mediation models revealed that PWB, but not stress, mediated the relationship between food security and BMI among Black/African American students. Regarding diet-related behaviors, high stress levels mediated the relationship between food insecurity and sugar-sweetened beverage intake among students.
Food insecurity appears to influence BMI in college students. This relationship seems to be mediated by disrupted PWB and a higher intake of sugar-sweetened beverages due to stress.
Abstract
We present very long baseline interferometry observations of a faint and low-luminosity (L1.4 GHz < 1027 W Hz−1) gigahertz-peaked spectrum (GPS) and compact steep-spectrum (CSS) sample. We ...select eight sources from deep radio observations that have radio spectra characteristic of a GPS or CSS source and an angular size of θ ≲ 2 arcsec, and detect six of them with the Australian Long Baseline Array. We determine their linear sizes, and model their radio spectra using synchrotron self-absorption (SSA) and free–free absorption (FFA) models. We derive statistical model ages, based on a fitted scaling relation, and spectral ages, based on the radio spectrum, which are generally consistent with the hypothesis that GPS and CSS sources are young and evolving. We resolve the morphology of one CSS source with a radio luminosity of $10^{25}\, \hbox{W Hz}^{-1}$, and find what appear to be two hotspots spanning 1.7 kpc. We find that our sources follow the turnover–linear size relation, and that both homogeneous SSA and an inhomogeneous FFA model can account for the spectra with observable turnovers. All but one of the FFA models do not require a spectral break to account for the radio spectrum, while all but one of the alternative SSA and power-law models do require a spectral break to account for the radio spectrum. We conclude that our low-luminosity sample is similar to brighter samples in terms of their spectral shape, turnover frequencies, linear sizes, and ages, but cannot test for a difference in morphology.
(1) Background: Systemic infection is associated with increased neuroinflammation and accelerated cognitive decline in AD patients. Activated neutrophils produce neutrophil-derived microvesicles ...(NMV), which are internalised by human brain microvascular endothelial cells and increase their permeability in vitro, suggesting that NMV play a role in blood-brain barrier (BBB) integrity during infection. The current study investigated whether microRNA content of NMV from AD patients is significantly different compared to healthy controls and could impact cerebrovascular integrity. (2) Methods: Neutrophils isolated from peripheral blood samples of five AD and five healthy control donors without systemic infection were stimulated to produce NMV. MicroRNAs isolated from NMV were analysed by RNA-Seq, and online bioinformatic tools were used to identify significantly differentially expressed microRNAs in the NMV. Target and pathway analyses were performed to predict the impact of the candidate microRNAs on vascular integrity. (3) Results: There was no significant difference in either the number of neutrophils (
= 0.309) or the number of NMV (
= 0.3434) isolated from AD donors compared to control. However, 158 microRNAs were significantly dysregulated in AD NMV compared to controls, some of which were associated with BBB dysfunction, including miR-210, miR-20b-5p and miR-126-5p. Pathway analysis revealed numerous significantly affected pathways involved in regulating vascular integrity, including the TGFβ and PDGFB pathways, as well as Hippo, IL-2 and DNA damage signalling. (4) Conclusions: NMV from AD patients contain miRNAs that may alter the integrity of the BBB and represent a novel neutrophil-mediated mechanism for BBB dysfunction in AD and the accelerated cognitive decline seen as a result of a systemic infection.
The type I receptor tyrosine kinases constitute a family of transmembrane proteins involved in various aspects of cell growth and survival and have been implicated in the initiation and progression ...of several types of human malignancies. The best characterized of these proteins are the epidermal growth factor receptor (EGFR) and ErbB-2 (HER-2/neu). We have developed potent quinazoline and pyrido-3,4-d-pyrimidine small molecules that are dual inhibitors of ErbB-2 and EGFR. The compounds demonstrate potent in vitro inhibition of the ErbB-2 and EGFR kinase domains with IC(50)s <80 nM. Growth of ErbB-2- and EGFR-expressing tumor cell lines is inhibited at concentrations <0.5 microM. Selectivity for tumor cell growth inhibition versus normal human fibroblast growth inhibition ranges from 10- to >75-fold. Tumor growth in mouse s.c. xenograft models of the BT474 and HN5 cell lines is inhibited in a dose-responsive manner using oral doses of 10 and 30 mg/kg twice per day. In addition, the tested compounds caused a reduction of ErbB-2 and EGFR autophosphorylation in tumor fragments from these xenograft models. These data indicate that these compounds have potential use as therapy in the broad population of cancer patients overexpressing ErbB-2 and/or EGFR.
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