Objective
To compare the effects of different implant placement and loading protocols on the marginal bone loss (MBL) in beagles by intraoral radiography.
Methods and materials
61 dental implants ...were inserted on 9 beagle dogs at bilateral lower posteriors according to 8 different protocols: immediate implant placement and immediate loading for 3 months (IIP + IL3) or 6 months (IIP + IL6) and unloading (IIP + UL), immediate implant placement and delayed loading for 3 months (IIP + DL3) or 6 months (IIP + DL6), delayed implant placement and immediate loading for 3 months (DIP + IL3) or delayed loading for 3 months (DIP + DL3) and unloading (DIP + UL). Intraoral radiography was performed to analyze the MBL during each surgery, before and after the implant placement and at 3-month intervals after the procedure.
Results
In total, 57 samples were included. There was less MBL (p<0.05) in the IIP + IL3 group (1.22 ± 0.63 mm) compared to the DIP + IL3 group (1.89 ± 0.9 mm). The longer the loading time, the more bone loss appeared in the IIP + IL group; however, the results were reversed in the IIP + DL group. The MBL during the latter 3-month period was dramatically decreased compared to the former 3-month period in the IIP + DL3 group (p<0.05).
Conclusions
The IIP + IL group seems superior to the DL protocol and the MBL changed significantly during the first three months and thereafter became stable.
Myocardial hypertrophy that often leads to eventual heart failure is a leading cause of mortality worldwide. While both apoptosis and cell proliferation have been reported to play an important part ...in heart failure, its exact triggering mechanism is still unclear. Reports have shown that low concentrations of H
2
O
2
(10–30 µM) can induce myocardial hypertrophy without affecting survival. The ubiquitin ligase Ube3a has been reported to have a close affiliation with Angelman syndrome; but many ubiquitin ligases have been reported in a variety of cardiovascular conditions including myocardial hypertrophy. However, the relationship between Ube3a and myocardial hypertrophy has never been reported in literature. The rat cardiac myoblast cell line H9c2 and primary neonatal cardiomyocytes showed similar hypertrophic responses in vitro. In this report, we utilized H
2
O
2
treatment on H9c2 cells to induce myocardial hypertrophy and determined the relationship between Ube3a and myocardial hypertrophy. Our results showed that 10–20 μM H
2
O
2
can induce myocardial hypertrophy without affecting cell viability and inducing cell apoptosis, while the corresponding transcription and translation levels of Ube3a are significantly increased during the process. Therefore, these findings underline that Ube3a may play an important role in myocardial hypertrophy.
Methicillin‐resistant Staphylococcus aureus (MRSA) can interfere with synergistic host defenses, thus escaping from immune surveillance. Herein, a new immunotherapy strategy‐simulating complement ...therapy is proposed. Two artificial effectors, Tat cell penetrating peptide and wheat germ agglutinin, are utilized to develop a multifunctional complement‐mimic liposome (CML) simultaneously delivering antibiotics and orchestrating the complement system with macrophages. The CML successfully simulates the functions of the complement system, including formation of a membrane attack complex, mediation of opsonophagocytosis, and activation of phagocytes. At infection sites, CMLs are able to recognize MRSA and disrupt bacterial permeation barriers (cell walls and cell membranes). Meanwhile, CMLs attached to the surface of MRSA are able to activate the phagocytosis and immune responses of macrophages. As a result, CMLs significantly increased the bacterial activity of clarithromycin both in vitro and in macrophages. Moreover, CMLs effectively reduced the MRSA burden in three infection models, including skin abscesses, pneumonia, and bacteremia mouse models. Therefore, the CML provides a novel strategy for overcoming bacterial immune evasion and sheds light on the development of immunotherapies for infectious diseases.
In this study, the complement‐mimic liposome (CML) is developed against methicillin‐resistant Staphylococcus aureus (MRSA) infection. The CML successfully simulates the functions of the complement system, including formation of a membrane attack complex, mediation of opsonophagocytosis, and activation of phagocytes. Importantly, intravenous administration of CMLs effectively reduces the MRSA burden in skin abscesses, pneumonia, and bacteremia mouse models.
The red-emitting phosphors of CaBi2Ta2O9: Eu3+ and CaBi2Ta2O9: Eu3+/La3+ were prepared by the high-temperature solid-state reaction method. By using X-ray diffraction, the structural properties of ...powders have been analyzed. The photoluminescence excitation and emission spectra of CaBi2Ta2O9: Eu3+, and the dependence of luminescence intensity on doped Eu3+ ions concentration were investigated. The results show that CaBi2Ta2O9: Eu3+ can be efficiently excited by near-UV and blue light to realize an intense red luminescence (615 nm) corresponding to the electric dipole transition 5D0 arrow right 7F2 of Eu3+ ions. Meantime, an enhancement of photoluminescence intensity was observed in La3+ ions doped CaBi2Ta2O9: Eu3+ phosphors. The CIE chromaticity coordinates of CaBi2Ta2O9: Eu3+ and CaBi2Ta2O9: Eu3+/La3+ are (0.6121, 0.3835) and (0.6141, 0.3821), corresponding to the hues of orange-red. The phosphors can be suggested to be a potential red-emitting phosphor for the application on white LEDs under irradiation of near-UV or blue chips.
The red-emitting phosphors of CaBi sub(2)Ta sub(2)O sub(9 ): Eu super(3+) and CaBi sub(2)Ta sub(2)O sub(9 ): Eu super(3+)/La super(3+) were prepared by the high-temperature solid-state reaction ...method. By using X-ray diffraction, the structural properties of powders have been analyzed. The photoluminescence excitation and emission spectra of CaBi sub(2)Ta sub(2)O sub(9 ): Eu super(3+), and the dependence of luminescence intensity on doped Eu super(3+) ions concentration were investigated. The results show that CaBi sub(2)Ta sub(2)O sub(9 ): Eu super(3+) can be efficiently excited by near-UV and blue light to realize an intense red luminescence (615 nm) corresponding to the electric dipole transition super(5)D sub(0) arrow right super(7)F sub(2) of Eu super(3+) ions. Meantime, an enhancement of photoluminescence intensity was observed in La super(3+) ions doped CaBi sub(2)Ta sub(2)O sub(9 ): Eu super(3+) phosphors. The CIE chromaticity coordinates of CaBi sub(2)Ta sub(2)O sub(9 ): Eu super(3+) and CaBi sub(2)Ta sub(2)O sub(9 ): Eu super(3+)/La super(3+) are (0.6121, 0.3835) and (0.6141, 0.3821), corresponding to the hues of orange-red. The phosphors can be suggested to be a potential red-emitting phosphor for the application on white LEDs under irradiation of near-UV or blue chips.
As novel amphiphilic materials, six uncharged alkyl rhamnosides incorporating various alkyl chain and one rhamnose amine quaternary ammonium salt were successfully synthesized in this study. Their ...amphiphilic properties (HLB and CMC), antimicrobial and anti-biofilm activity against S. aureus and P. aeruginosa were investigated. Differentially regulated proteins and pathways were identified by comparative proteomics research to first give a sight on how alkyl rhamnosides performed the anti-biofilm activity at protein and pathway levels. Among the uncharged alkyl rhamnosides, dodecyl rhamnoside and octyl rhamnoside showed the best antimicrobial and anti-biofilm ability against S. aureus and against P. aeruginosa, respectively. Interestingly, the relationships between amphiphilic properties or MIC with anti-biofilm activity were first established. Uncharged alkyl rhamnoside with an optimized HLB value of 5.0 had both the strongest antibacterial and anti-biofilm activity against S. aureus, and MIC was the maximum biofilm inhibitory concentration for all alkyl rhamnosides. Alkyl rhamnosides have a significant overall regulatory effect on the proteomics and pathways of bacterial biofilms, including energy production, substrates transportation, signal transduction, key molecules balance, and so on. These amphiphilic materials have a great potential to be used as additives in pharmaceutic, cosmetic, food industry, hospital and in other non-medical fields.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Myocardial hypertrophy that often leads to eventual heart failure is a leading cause of mortality worldwide. While both apoptosis and cell proliferation have been reported to play an important part ...in heart failure, its exact triggering mechanism is still unclear. Reports have shown that low concentrations of H sub(2)O sub(2) (10-30 mu M) can induce myocardial hypertrophy without affecting survival. The ubiquitin ligase Ube3a has been reported to have a close affiliation with Angelman syndrome; but many ubiquitin ligases have been reported in a variety of cardiovascular conditions including myocardial hypertrophy. However, the relationship between Ube3a and myocardial hypertrophy has never been reported in literature. The rat cardiac myoblast cell line H9c2 and primary neonatal cardiomyocytes showed similar hypertrophic responses in vitro. In this report, we utilized H sub(2)O sub(2) treatment on H9c2 cells to induce myocardial hypertrophy and determined the relationship between Ube3a and myocardial hypertrophy. Our results showed that 10-20 mu M H sub(2)O sub(2) can induce myocardial hypertrophy without affecting cell viability and inducing cell apoptosis, while the corresponding transcription and translation levels of Ube3a are significantly increased during the process. Therefore, these findings underline that Ube3a may play an important role in myocardial hypertrophy.
Myocardial hypertrophy that often leads to eventual heart failure is a leading cause of mortality worldwide. While both apoptosis and cell proliferation have been reported to play an important part ...in heart failure, its exact triggering mechanism is still unclear. Reports have shown that low concentrations of H.sub.2O.sub.2 (10-30 microM) can induce myocardial hypertrophy without affecting survival. The ubiquitin ligase Ube3a has been reported to have a close affiliation with Angelman syndrome; but many ubiquitin ligases have been reported in a variety of cardiovascular conditions including myocardial hypertrophy. However, the relationship between Ube3a and myocardial hypertrophy has never been reported in literature. The rat cardiac myoblast cell line H9c2 and primary neonatal cardiomyocytes showed similar hypertrophic responses in vitro. In this report, we utilized H.sub.2O.sub.2 treatment on H9c2 cells to induce myocardial hypertrophy and determined the relationship between Ube3a and myocardial hypertrophy. Our results showed that 10-20 muM H.sub.2O.sub.2 can induce myocardial hypertrophy without affecting cell viability and inducing cell apoptosis, while the corresponding transcription and translation levels of Ube3a are significantly increased during the process. Therefore, these findings underline that Ube3a may play an important role in myocardial hypertrophy.
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