The study was aimed to determine fluoride levels in plasma, brain, and bones of Wistar rats following chronic administration of fluoride at different dose levels and the consequent oxidative damage ...inflicted in these tissues. Brain histomorphology and bone radiographs were also evaluated to assess the extent of damage in these organs. Eighteen rats were randomly divided into three groups with six animals in each group. Group I served as control and groups II and III received 50 and 100 ppm fluoride in tap water, respectively for 180 days. A dose-dependent rise in the levels of fluoride in plasma, brain, and bones was observed in rats. Significant (P < 0.05) alterations in levels of total thiols, glutathione peroxidase, glutathione reductase, acetylcholinesterase, catalase, superoxide dismutase, lipids, as well as protein peroxidation in blood and brain were observed as compared to control in a dose-dependent manner. Radiological examination of bone revealed thinning of bone cortex with haphazard ossification, reduced bone density, and widening of marrow cavity indicating occurrence of flawed bone remodeling upon chronic fluoride exposure. Improper mineralization in bones of intoxicated rats indirectly reflected reduced bone tensile strength. Moreover, alterations in plasma Ca:P ratio and high levels of fluoride in bone ash indicated that chronic fluoride exposure leads to alterations in the bone matrix further corroborating the radio-graphical findings. Additionally, severe microscopic alterations were recorded in the cerebrum and cerebellum of treated rats which included neuronal necrosis, gliosis, spongiosis, perivascular cuffing, congestion, and hemorrhage which correlated well with oxidative changes induced by fluoride intoxication in the brain tissue of rats.
Nanofluids (NFs) incorporation has emerged as a pivotal advancement in the realm of technology. With the advancement in technology, new segments of NFs are introduced recently. Compared to ...conventional fluids, both NFs and Hybrid nanofluids (HNFs) offer significantly enhanced heat transfer due to the presence of nanoparticles (NPs). NFs provide an initial boost, but HNFs take it a step further by combining different NPs for even greater thermal performance and efficiency. HNFs are combination of two or more NPs dispersed in convectional fluid. This compilation purpose is to serve as a succinct overview covering HNFs history, preparation techniques, thermo-physical properties, future directions, current status and applications. Mainly two-step and one-step methods are discussed for preparation. Thermophysical properties of HNFs like thermal conductivity, specific heat, density and viscosity for distinct parameter variation (temperature, concentration etc.) are reviewed. HNFs applications in various fields like solar heating systems, cooling systems, heat exchangers and industrial processes, medical treatments, enhanced oil recovery have been discussed. The future aspects focus on refining NPs compositions, scalable production methods, eco-friendly formulations and optimizing predictive models for diverse applications.
Concurrent exposure to a multitude of environmental toxicants pose serious health hazard to humans and animals. The present investigation was conceptualized to determine deleterious effects of ...concomitant subacute arsenic and quinalphos exposure on antioxidant responses of liver and erythrocytes of Wistar rats. Fifty-four Wistar rats were divided into nine groups with six animals in each. Animals were exposed to either quinalphos (1/100
th
and 1/10
th
of LD
50
) through oral gavage daily or arsenic (50 and 100 ppb) in drinking water alone and in combination for 28 days. While treatment with different toxicants alone also significantly reduced hemoglobin concentration, hepatic biomarkers and levels of antioxidant parameters as compared with control values, concomitant exposure significantly (
P
< 0.05) elevated levels of hepatic transaminases and alkaline phosphatase. Moreover, along with significant depletion in activities of SOD, CAT, TTH, AChE, and enzymes of glutathione complex, a significant enhancement of lipid peroxidation was also recorded in liver and erythrocytes in co-exposed animals in a dose-dependent manner when compared with exposure to individual toxicant. More severe alterations occurred in hepatic histo-architecture of rats receiving combined treatment as compared with those treated with either toxicant. Results indicated that oxidative damage in erythrocytes was more than that of the liver of rats on concomitant exposure of arsenic and quinalphos in a dose-dependent manner. In nutshell, our results revealed that combined treatment of quinalphos with arsenic potentiated toxic effects of either toxicant on antioxidant machinery of liver and erythrocytes and hepatic histomorphology of exposed Wistar rats.
Improving fiber quality and yield are the primary research objectives in cotton breeding for enhancing the economic viability and sustainability of Upland cotton production. Identifying the ...quantitative trait loci (QTL) for fiber quality and yield traits using the high-density SNP-based genetic maps allows for bridging genomics with cotton breeding through marker assisted and genomic selection. In this study, a recombinant inbred line (RIL) population, derived from cross between two parental accessions, which represent broad allele diversity in Upland cotton, was used to construct high-density SNP-based linkage maps and to map the QTLs controlling important cotton traits.
Molecular genetic mapping using RIL population produced a genetic map of 3129 SNPs, mapped at a density of 1.41 cM. Genetic maps of the individual chromosomes showed good collinearity with the sequence based physical map. A total of 106 QTLs were identified which included 59 QTLs for six fiber quality traits, 38 QTLs for four yield traits and 9 QTLs for two morphological traits. Sub-genome wide, 57 QTLs were mapped in A sub-genome and 49 were mapped in D sub-genome. More than 75% of the QTLs with favorable alleles were contributed by the parental accession NC05AZ06. Forty-six mapped QTLs each explained more than 10% of the phenotypic variation. Further, we identified 21 QTL clusters where 12 QTL clusters were mapped in the A sub-genome and 9 were mapped in the D sub-genome. Candidate gene analyses of the 11 stable QTL harboring genomic regions identified 19 putative genes which had functional role in cotton fiber development.
We constructed a high-density genetic map of SNPs in Upland cotton. Collinearity between genetic and physical maps indicated no major structural changes in the genetic mapping populations. Most traits showed high broad-sense heritability. One hundred and six QTLs were identified for the fiber quality, yield and morphological traits. Majority of the QTLs with favorable alleles were contributed by improved parental accession. More than 70% of the mapped QTLs shared the similar map position with previously reported QTLs which suggest the genetic relatedness of Upland cotton germplasm. Identification of QTL clusters could explain the correlation among some fiber quality traits in cotton. Stable and major QTLs and QTL clusters of traits identified in the current study could be the targets for map-based cloning and marker assisted selection (MAS) in cotton breeding. The genomic region on D12 containing the major stable QTLs for micronaire, fiber strength and lint percentage could be potential targets for MAS and gene cloning of fiber quality traits in cotton.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
This study was designed to determine alterations in renal biomarkers, antioxidant profile, and histomorphology of renal tissue following subacute exposure to quinalphos alone or in conjunction with ...arsenic in rats. A total of 54 adult Wistar rats were randomly divided into nine groups of six rats each and were administered sub-lethal concentrations of quinalphos (1/100
th
and 1/10
th
of LD
50
) orally daily and arsenic (50 and 100 ppb) in drinking water for 28 days. Significantly (
p
< 0.05) decreased levels of antioxidant biomarkers in renal tissue, viz., total thiols, catalase, superoxide dismutase, glutathione peroxidase, glutathione-s-transferase, and glutathione reductase along with increased (
p
< 0.05) thiobarbituric acid reacting substance (TBRAS) levels indicated that significant oxidative damage to renal tissue occurred following repeated administrations of quinalphos at either dose levels or arsenic at the concentration of 100 ppb when compared with the control rats. The alterations in the antioxidant parameters were observed to be more pronounced in co-administered groups as compared with either toxicant administered group. Similarly, activity of renal acetylcholinesterase was decreased after repeated exposure to quinalphos or arsenic, but inhibition was higher (up to 48%) in rat renal tissue co-exposed with quinalphos and arsenic at the higher concentration. These findings corroborated with the histopathological alterations in renal tissue of toxicant exposed rats. The altered plasma and tissue antioxidant biomarkers along with histopathological changes in the kidney at higher dose level of either toxicant indicate that renal tissue is significantly impacted by these toxicants, and these effects become more pronounced after their co-administration.
Imidacloprid (IMI), a newer neonicotinoid insecticide, induces oxidative insult to hepatocytes due to the formation of reactive metabolites during hepatic metabolism. The present study aimed to ...determine the potentiating effect of arsenic (As) on IMI-induced hepatic damage in Wistar rats. Rats, randomly divided into eight groups with six in each, were subjected to daily oral administration for 28 days. Group I served as control; group II received IMI at the dose rate of 16.9 mg/kg body weight; groups III, IV, and V received As at the dose rate of 50, 100, and 150 ppb, respectively, in drinking water; groups VI, VII, and VIII received both IMI (16.9 mg/kg) and As in drinking water at the rate of 50, 100, and 150 ppb, respectively. Repeated oral administration of IMI or As resulted in significant (p < 0.05) elevation of plasma phosphatases, transferases, hepatic malondialdehyde, and advanced oxidation protein product levels, but significantly (p < 0.05) decreased levels of total proteins, thiols, and activities of antioxidant enzymes that indicate oxidation-induced hepatotoxicity. These findings were further corroborated by histological alterations in hepatic tissue of IMI or As-administered rats. The coadministration of both IMI and As in rats produced more severe alterations in these parameters in hepatic tissue. Reduced antioxidant indices and increased hepatic damage biomarkers with pronounced histopathological alterations in hepatic tissue after combined exposure to toxicants indicate potentiating toxic effect of As on IMI-induced hepatotoxicity.
Abstract
Goat warble fly infestation (GWFI) is a subcutaneous myiasis caused by larvae of
Przhevalskiana silenus
, an insect belonging to the order Diptera
.
The diagnosis of GWFI is challenging in ...the early larval instars (L1 and L2) as they are occult under the skin and hair coat causing prolonged economic loss in form of meat and hide damage. This necessitates early diagnosis for disease control at herd level and its prophylactic management to prevent economic losses. Hypodermins, a class of serine proteases from Hypoderminae subfamily have been used as serodiagnostic antigens for the past four decades for diagnosis of warble fly myiasis. In this study,the immunodominant antigen Hypodermin C (HyC) from
P. silenus
has been recombinantly expressed in
E. coli
and immunogenic characterisation of expressed protein was done. The protein shows hallmark residues in conserved cysteine and catalytic triad typical of serine proteases along with similar profile of immunoreactivity towards Hypoderminae infestation. The present study reports an optimised indirect-ELISA based on recombinant HyC derived from
P. silenus
for early diagnosis of GWFI. The optimised indirect ELISA provides a sensitive and specific immunodiagnostic for mass surveillance of the GWFI with diagnostic specificity and sensitivity of 96% and 100%, respectively and not showing any cross reactivity against other important parasitic and bacterial diseases of goats. This study presents the first report of indirect ELISA based on recombinant Hypodermin C antigen derived from
P. silenus
for the serosurveillance of goat warble fly disease
.
•High dose of APAP induced hepatic and renal impairment in rats.•B. ciliata extract alleviated APAP-induced altered biochemical and oxidative indices.•The extract also reversed the histopathological ...alterations in erythrocytes, liver and kidney.•The presence of antioxidants in the extract afforded protection to the liver and kidney.
Background: Bergenia ciliata (Haw.) Sternb. is a medicinal plant bestowed with antioxidative phytochemicals and has been traditionally associated with several medicinal and health-promoting properties and used in the management of diseases.
Method: The study was undertaken to determine the potential of the extract of the rhizome of Bergenia ciliata (Haw.) Sternb. at 150 and 300 mg/kg body weight (BW) to attenuate the drug-induced oxidative damage in hepatic and renal tissues of Wistar rats. The toxicity was induced by a single oral administration of acetaminophen (APAP).
Results: The oral administration of APAP significantly altered the levels of hepatic and renal damage indicators in Wistar rats. The levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-s-transferase (GST) and total thiols (TTH) decreased significantly in erythrocytes, hepatic and renal tissues. Administration of B. ciliata (150 mg/kg BW) significantly decreased the elevated hepato-renal biomarkers and malondialdehyde (MDA) levels in the tissues whereas significantly increased the levels of SOD, GST and TTH in erythrocytes and liver. The higher dose of B. ciliata (300 mg/kg BW) restored the levels of plasma hepatic and renal biomarkers as well as antioxidant parameters (SOD, GST, GPx, TTH and MDA). It also reversed the histopathological alterations in hepato-renal tissues indicating the protective potential of the extract.
Conclusions: Overall, the results indicated that the B. ciliata rhizome extract attenuated the drug-induced hepato-renal toxicity in a dose-dependent manner in Wistar rats and therefore, has the potential for future therapeutic uses.
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The steady two-dimensional boundary layer stagnation point flow due to a shrinking sheet is analyzed. The combined effects of magnetic field and nonlinear convection are taken into account. The ...governing equations for the flow are modeled and then simplified using the similarity transformation and boundary layer approach. The numerical solution of the reduced equations is obtained by the second-order finite difference scheme also known as Keller box method. The influence of the pertinent parameters of the problem on velocity and temperature profiles, skin friction, and sheet temperature gradient are presented through the graphs and tables and discussed. The magnetic field and nonlinear convection parameters significantly enhance the solution range.
•Mancozeb-arsenic co-exposure exacerbated oxidative stress and histopathological alterations in rats.•Quercetin and catechin showed strong ameliorative potential against the combined toxicity.•Both ...attenuated the alterations in oxidative biomarkers in the blood and brain.•Shielded histomorphology of the brain against oxidative damage.
The study was designed to determine the ameliorative potential of quercetin and catechin against the toxicity induced by co-exposure to Mancozeb (MZ) and arsenic in Wistar rats. Sixty-adult male Wistar rats were randomly divided into 10 groups (n=6). Group I served as control and group II was exposed to MZ 800 mg/kg, PO (per OS). Groups III, IV, and V were given drinking water containing sodium arsenite at the rate of 10, 50, and 100 ppb, respectively, for 28 days. Groups VI, VII, and VIII were treated with sodium arsenite in drinking water at 10, 50, and 100 ppb, respectively, along with MZ (800 mg/kg) for 28 days. Groups IX and X were given drinking water mixed with sodium arsenite at 100 ppb, MZ at 800 mg/kg, and quercetin (50 mg/kg) and catechin (50 mg/kg), respectively. Co-exposure to the toxicants (MZ and arsenic) significantly (P<0.05) aggravated the oxidative stress-induced alterations and the histopathological changes induced by individual toxicants. Supplementation of quercetin or catechin markedly attenuated the variations in nitric oxide levels and oxidative biomarkers in blood and the brain and shielded the histomorphology of the brain against oxidative damage.
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