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•A new co-amorphous material for the drug tolbutamide with tromethamine.•Stable co-amorphous with a small molecular as a coformer.•Solubility of tolbutamide increased 2.5 ...fold.•Spectral, thermodynamic data and electronic properties calculated by DFT.
Among the strategies for bioavailability improvement of poorly soluble drugs, co-amorphous systems have revealed to have a significant impact in the increase of the aqueous solubility of the drug, and at the same time increasing the amorphous state stability and dissolution rate when compared with the neat drug. Tolbutamide (TBM) is an oral hypoglycemic drug largely used in the treatment of type II Mellitus diabetes. TBM is a class II drug according to the Biopharmaceutical Classification System, meaning that it has low solubility and higher permeability. The aim of this study was to synthesize a co-amorphous material of tolbutamide (TBM) with tromethamine (TRIS). Density functional theory (DFT), allowed to study the structural, electronic, and thermodynamic properties, as well as solvation effects. In same theory level, several interactions tests were performed to obtain the most thermodynamically favorable drug-coformer intermolecular interactions. The vibrational spectra (mid infrared and Raman spectroscopy) are in accordance with the theoretical studies, showing that the main molecular interactions are due to the carbonyl, sulfonyl, and amide groups of TMB and the alcohol and amine groups of TRIS. X-ray powder diffraction was used to study the physical stability in dry condition at 25 °C of the co-amorphous system, indicating that the material remained in an amorphous state up to 90 days. Differential scanning calorimetry and thermogravimetric results showed a high increase of the Tg when compared with the amorphous neat drug, from 4.3 °C to 83.7 °C, which generally translated into good physical stability. Solubility studies demonstrated an increase in the solubility of TBM by 2.5 fold when compared with its crystalline counterpart.
Hemangiopericytoma/solitary fibrous tumor (HPC/STF) is a rare tumor arising from Zimmerman’s pericytes and it is characterized by an aggressive malignancy, with a high tendency for local and distant ...recurrence. The authors report the case of a middle-aged woman with HPC/SFT of the right parietal bone, which is an extremely rare primary location of involvement. The patient presented with a painful deformity of insidious growth at the right parietal region. Assessment with cranial computed tomography scan and magnetic resonance imaging revealed an expansive lesion at the right parietal bone, with exocranial extension and 27 mm of maximal diameter. Craniotomy with gross tumor removal, duraplasty, and cranioplasty was performed, and the diagnosis of HPC/SFT, WHO grade III, was established by pathological and immunohistochemical analysis. The patient was then evaluated for adjuvant radiation therapy and received a dose of 60 Gy (2 Gy/fraction) with 3D conformal radiotherapy to the surgical bed. The adjuvant treatment was uneventful and, after 8 months of follow-up, there was no suspected local or distant recurrence. The rarity of this diagnosis, its aggressive behavior, and the lack of published data posed several challenges for the treatment management.
Cannabidiol (CBD) is one of the main components of Cannabis sativa and has a wide spectrum of action, including effects in the sleep-wake cycle.
Objective:
The objective of this paper is to assess ...the effects on sleep of acute systemic administration of CBD.
Method:
Adult male Wistar rats were randomly distributed into four groups that received intraperitoneal injections of CBD 2.5 mg/kg, CBD 10 mg/kg, CBD 40 mg/kg or vehicle (n=seven animals/group). Sleep recordings were made during light and dark periods for four days: two days of baseline recording, one day of drug administration (test), and one day after drug (post-test).
Results:
During the light period of the test day, the total percentage of sleep significantly increased in the groups treated with 10 and 40 mg/kg of CBD compared to placebo. REM sleep latency increased in the group injected with CBD 40 mg/kg and was significantly decreased with the dose of 10 mg/kg on the post-test day. There was an increase in the time of SWS in the group treated with CBD 40 mg/kg, although this result did not reach statistical significance.
Conclusion:
The systemic acute administration of CBD appears to increase total sleep time, in addition to increasing sleep latency in the light period of the day of administration.
Objective
Women with gynecologic cancer may suffer from pelvic floor dysfunction (PFD). Before radiotherapy, prehabilitation with pelvic floor muscle exercises (PFME) and vaginal dilator (VD) might ...prevent it and foster sexual life. This study aims to explore the experience of gynecologic cancer patients getting external beam radiation treatments regarding barriers to and facilitators of adherence to a prehabilitation program to prevent PFD.
Methods
This qualitative research with thematic content analysis included 11 women with gynecologic cancer and different levels of adherence to PFME and VD. Participants were interviewed based on a semi-structured script. The information was analyzed manually, assisted with Nvivo12® software, and triangulated with open coding.
Results
High self-motivation, desire to improve their health, symptoms of improvement, availability of time, the desire to resume sexual life, and the support of the partner were facilitators of adherence. The instructional exercise audio, clarity of the information, and closer communication with the physical therapist were also valued. The main barriers were general malaise secondary to oncological treatments, forgetfulness, lack of time, misinformation, lack of coordination with the treatment team, discomfort with the VD, and a feeling of shame. Feedback from the attending physician was a facilitator when present or a barrier when absent.
Conclusion
These barriers and facilitators should be considered when designing and implementing preventive programs with PFME and VD. Behavioral counselling should consider the desire to remain sexually active; in such cases, including the partner in the therapeutic process is appraised. Otherwise, the focus should be on benefits for maintenance of pelvic floor function.
ABSTRACT We evaluated the technical feasibility of purified glycerin inclusion in balanced diets of broiler chickens treated from 1 to 42 days of age. A total of 240 broiler chickens were distributed ...in a completely randomized design into four treatments (0, 2, 4, and 6% purified glycerin inclusion), with six replicates of 10 broilers each. We evaluated productive performance (at 7, 21, and 42 days), edible viscera (heart, liver, and gizzard), carcass yield and cuts, color, chemical and physical composition, as well as protein and fat deposition in the breast muscle. Dietary inclusion of purified glycerin reduced feed conversion and increased feed intake, weight gain, and weight at seven days. From 1 to 21 days, there was a decrease in feed conversion and a linear increase in weight gain, with no effect on feed intake. Considering the total experimental period, increasing glycerin levels increased weight gain and weight at 42 days, with no effects on feed conversion or feed intake. Similarly, there was no effect on carcass and cut yields, liver and gizzard yields, and weight or length of the intestine, while heart yield was decreased. There was a linear increase in crude protein, ether extract, protein, and fat deposition and a reduction in moisture percentages, with no effect on ash content. Purified glycerin levels did not affect a*, b*, L*, pH, temperature, shear force, or weight loss by cooking in breast meat. The dietary inclusion of purified glycerin of up to 6% proved to be technically feasible in the diets of broilers from 1 to 42 days.
Background
The IL7RA polymorphisms have recently been associated with CD4+ T‐cell decline in untreated HIV‐infected subjects and CD4+ T‐cell recovery in patients on combination antiretroviral therapy ...(cART). The aim of this study was to evaluate whether IL7RA polymorphisms are associated with CD4+ T‐cell recovery in HIV‐infected patients on long‐term cART.
Study design
We performed a retrospective study in 151 naïve cART patients with severe immunodeficiency (CD4+ counts ≤200 cells/mm3). IL7RA polymorphisms' genotyping was performed using Sequenom's MassARRAY platform. The outcome variable was the time to achieve the first value of CD4+ count ≥500 cells/mm3 during the follow‐up.
Results
Two different trends of CD4+ T‐cell recovery were found in Kaplan–Meier analysis. During the first 48 months, 60 of 151 (39·7%) of the patients reached CD4+ T‐cell values ≥500 cells/mm3, and no differences were observed between IL7RA genotypes. After the first 48 months of follow‐up, 27 of 151 (17·8%) of the patients reached CD4+ T‐cell values ≥500 cells/mm3, with a different pattern of CD4+ recovery depending on IL7RA genotype. Patients with rs10491434 TT genotype and rs6897932 TT genotype were more likely of achieving CD4+ value ≥500 cells/mm3 than patients with rs10491434 CT/CC genotype (adjusted hazard ratio (aHR) = 3·59; P = 0·005) and patients with rs6897932 CC/CT genotype (aHR = 11·7; P < 0·001).
Conclusions
The IL7RA polymorphisms seem to be associated with CD4+ T‐cell recovery in HIV‐infected patients who started cART with severe immunodeficiency, in the second phase of CD4+ T‐cell recovery after long‐term cART.
Associations of tendon injuries with ankle and hindfoot fractures is a current concern and recent research has linked specific fractures with specific tendon injuries, despite its functional impact ...being unknown. The purpose of this study is to assess that impact.
Computed tomography scans of patients with ankle and hindfoot fractures were reviewed for tendon injuries. Patients were clinically evaluated with the Visual Analogue Scale (VAS) and the Manchester–Oxford Foot Questionnaire (MOXFQ), via a telephonic interview.
Eighty-five patients were included in the study. The mean age was of 49.9 ± 16.5 (14–80) years and there were 43 females (50,6%). Mean follow-up was of 43,7 ± 15,1 (24,4–82,2) months. Tendon injuries were identified in 23 patients (27,1%) and the most common lesion was tendon entrapment. The tibialis posterior tendon (TP) was injured in 18 patients (21,2%) and comprised 58,1% of all tendon injuries. Tendon injuries were mostly associated with pilon fractures, which was the most common fracture type (44,7%). There was no clinical difference between patients with a tendon injury VAS of 4,3 ± 2,6 (0–8) and MOXFQ score of 35,1 ± 22,4 (5–80), and those without VAS of 3,9 ± 2,5 (0–10) and MOXFQ of 34,3 ± 26,0 (0–95), respectively (p = 0,281 and 0,689).
Tendon injuries were present in 27% of patients with ankle and hindfoot injuries. The most frequently injured tendon was the TP in association with pilon fractures, with entrapment being the most common lesion. These lesions, however, do not seem to reflect in a worse functional outcome at two years of follow-up.
ABSTRACT We investigated the effect of Alpinia zerumbet essential oil on the quality and shelf life of tambaqui (Colossoma macropomum) fillets stored under refrigeration (10.0 ± 0.5 °C) for 14 days. ...The treatments were A. zerumbet essential oil at 0.75% v v-1 (AEO 0.75%), A. zerumbet essential oil at 1.5% v v-1 (AEO 1.5%) and a control (no essential oil). The sample quality and shelf life were determined by the total psychrotrophic count (TPC) and chemical parameters (pH, total volatile basic nitrogen, centesimal composition and thiobarbituric acid reactive substances - TBARS) at zero, seven and 14 days of storage time. The TPC decreased significantly (p < 0.05) with an A. zerumbetessential oil level of 1.5% until seven days of storage. The concentration of A. zerumbet essential oil at 0.75% resulted in lower pH, TBARS, and TVBN values in comparison with the other treatment and the control. Thus, A. zerumbet essential oil was efficient in extending the shelf life of refrigerated tambaqui fillets up to approximately seven days.
RESUMO Investigamos o efeito do óleo essencial de Alpinia zerumbet sobre a qualidade e vida útil de filés de tambaqui (Colossoma macropomum) armazenados sob refrigeração (10,0 ± 0,5 °C) durante 14 dias. Os tratamentos foram óleo essencial de A. zerumbet (OEA) a 0,75% v v-1 (OEA 0,75%) e 1,5% v v 1 (OEA 1,5%), além de um controle (sem OEA). A qualidade e vida útil das amostras foram determinadas por contagem psicrotrófica total (CPT) e parâmetros químicos (pH, bases nitrogenadas voláteis totais, composição centesimal e substâncias reativas ao ácido tiobarbitúrico - SRATB), nos dias zero, sete e 14 de armazenamento. O TPC diminuiu significativamente (p < 0,05) com óleo essencial de A. zerumbet a 1,5% por até sete dias de armazenamento. O tratamento contendo OEA a 0,75% resultou em menores valores de pH, SRTAB e BNV-T em comparação ao outro tratamento e ao controle. Portanto, o óleo essencial de A. zerumbet foi eficiente para prolongar a vida útil do filé de tambaqui por aproximadamente sete dias.
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