Impact of low-dose electron irradiation on n+p silicon strip sensors Adam, W.; Baulieu, G.; Boudoul, G. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
12/2015, Letnik:
803, Številka:
C
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The response of n+p silicon strip sensors to electrons from a 90Sr source was measured using a multi-channel read-out system with 25ns sampling time. The measurements were performed over a period of ...several weeks, during which the operating conditions were varied. The sensors were fabricated by Hamamatsu Photonics on 200μm thick float-zone and magnetic-Czochralski silicon. Their pitch was 80μm, and both p-stop and p-spray isolation of the n+ strips were studied. The electrons from the 90Sr source were collimated to a spot with a full-width-at-half-maximum of 2mm at the sensor surface, and the dose rate in the SiO2 at the maximum was about 50Gy(SiO2)/d. After only a few hours of making measurements, significant changes in charge collection and charge sharing were observed. Annealing studies, with temperatures up to 80°C and annealing times of 18h showed that the changes can only be partially annealed. The observations can be qualitatively explained by the increase of the positive oxide-charge density due to the ionization of the SiO2 by the radiation from the β source. TCAD simulations of the electric field in the sensor for different oxide-charge densities and different boundary conditions at the sensor surface support this explanation. The relevance of the measurements for the design of n+p strip sensors is discussed.
Protein phosphorylation is one of the most important known posttranslational modifications. Tandem mass spectrometry has become an important tool for mapping out the phosphorylation sites. However, ...when a peptide generated from the enzymatic or chemical digestion of a phosphoprotein is highly phosphorylated or contains many potential phosphorylation residues, phosphorylation site assignment becomes difficult. Separation and enrichment of phosphopeptides from a digest mixture is desirable and often a critical step for MS/MS-based site determination. In this work, we present a novel open tubular immobilized metal ion affinity chromatography (OT-IMAC) method, which is found to be more effective and reproducible for phosphopeptide enrichment, compared to a commonly used commercial product, Ziptip from Millipore. A strategy based on a combination of OT-IMAC, sequential dual-enzyme digestion, and matrix-assisted laser desorption/ionization (MALDI) quadrupole time-of-flight tandem mass spectrometry for phosphoprotein characterization is presented. It is shown that MALDI MS/MS with collision-induced dissociation can be very effective in generating fragment ion spectra containing rich structural information, which enables the identification of phosphorylation sites even from highly phosphorylated peptides. The applicability of this method for real world applications is demonstrated in the characterization and identification of phosphorylation sites of a Na+/H+ exchanger fusion protein, His182, which was phosphorylated in vitro using the kinase Erk2.
Test beam results of 3D silicon pixel sensors for the ATLAS upgrade Grenier, P.; Alimonti, G.; Barbero, M. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
05/2011, Letnik:
638, Številka:
1
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Results on beam tests of 3D silicon pixel sensors aimed at the ATLAS Insertable B-Layer and High Luminosity LHC (HL-LHC) upgrades are presented. Measurements include charge collection, tracking ...efficiency and charge sharing between pixel cells, as a function of track incident angle, and were performed with and without a 1.6
T magnetic field oriented as the ATLAS inner detector solenoid field. Sensors were bump-bonded to the front-end chip currently used in the ATLAS pixel detector. Full 3D sensors, with electrodes penetrating through the entire wafer thickness and active edge, and double-sided 3D sensors with partially overlapping bias and read-out electrodes were tested and showed comparable performance.
The ultimate goal of a team of two is the overriding desire to build, to serve, to perform, to grow, and to teach. At its highest level, team-based leaders and team-led organizations regardless of ...size, location, or context are followed, imitated, and respected because of who they are and the values their organizations represent. Servant leadership, within a team framework, is becoming the preferred way of galvanizing the workforce in the contemporary, service driven environment. The team of two described in this article systematically and operationally managed alignment, coordination, continuous learning, power, interpersonal interactions, and the decision process by creating and maintaining mutual trust, accountability, and respect. In effect, the “reflective practitioners” highlighted in this analysis demonstrate that actions do speak louder than words.
The ultimate goal of a team of two is the overriding desire to build, to serve, to perform, to grow, and to teach. At its highest level, team-based leaders and team-led organizations regardless of ...size, location, or context are followed, imitated, and respected because of who they are and the values their organizations represent. Servant leadership, within a team framework, is becoming the preferred way of galvanizing the workforce in the contemporary, service driven environment. The team of two described in this article systematically and operationally managed alignment, coordination, continuous learning, power, interpersonal interactions, and the decision process by creating and maintaining mutual trust, accountability, and respect. In effect, the "reflective practitioners" highlighted in this analysis demonstrate that actions do speak louder than words.
The ultimate goal of a team of two is the overriding desire to build, to serve, to perform, to grow, and to teach. At its highest level, team-based leaders and team-led organizations regardless of ...size, location, or context are followed, imitated, and respected because of who they are and the values their organizations represent. Servant leadership, within a team framework, is becoming the preferred way of galvanizing the workforce in the contemporary, service driven environment. The team of two described in this article systematically and operationally managed alignment, coordination, continuous learning, power, interpersonal interactions, and the decision process by creating and maintaining mutual trust, accountability, and respect. In effect, the "reflective practitioners" highlighted in this analysis demonstrate that actions do speak louder than words.
We describe a microspot matrix-assisted laser desorption ionization (MALDI) mass spectrometric approach to analyze gel-separated phosphoproteins. This method involves in-gel digestion of ...phosphoproteins after gel separation, followed by open tubular capillary (OTC) immobilized metal-ion affinity chromatography (IMAC) to capture the phosphopeptides with markedly reduced interferences from nonphosphorylated peptides. Nanoliter-volume of ammonium phosphate is used to elute the phosphopeptides captured on the capillary tube. After mixing with a small volume of matrix solution in the capillary, the effluent is deposited in a microspot on a sample plate for MALDI−MS analysis. It is also shown that, with peptide esterification after in-gel digestion of a phosphoprotein, negative ion detection in MALDI gives a distinct advantage over the positive ion mode of operation for phosphopeptide analysis, even without IMAC enrichment. However, the OTC−IMAC technique is demonstrated to be superior to the approach of negative ion detection of esterified in-gel digests without IMAC. OTC−IMAC is found to be sufficiently selective to capture phosphopeptides from in-gel digest of a gel band containing predominately one protein and the combination of peptide esterification and IMAC enrichment does not provide any real advantage. Using a standard phosphoprotein α-casein as a model system, we demonstrate that this OTC−IMAC method can detect a number of phosphopeptides after in-gel digestion with mid-fmol protein sample loading. An example of real world applications of this method is illustrated in the characterization of a fusion protein, His182, expressed in E. coli. Keywords: phosphoprotein • open tubular column • IMAC • MALDI
Capital investment in the United States health care industry is one of the components of the system that has historically fueled cost increases and complicated the quality/cost/access conundrum. This ...article is concerned with capital development focused on clinical equipment acquisition in acute care hospitals. The research findings suggest that decision-makers are abandoning two approaches to decision-making known as Quantitative Decision-Making and Mixed Scanning in favor of two models known as Rational Decision-Making and Political Decision-Making. While this is the case, a method of decision-making known as Idea Sets (developed and labeled by James March as Garbage Can Decision-Making) is the most widely used approach. Equipment acquisition criteria used by the decision-makers are shifting away from concerns of enhancing existing clinical programs and/or adding new clinical programs. Acquisition criteria are shifting toward procuring equipment which will decrease institutional expense, improve organizational efficiency, and galvanize operational effectiveness. In addition to the technical findings of the research, further insights about ourselves and our colleagues are gleaned. Accordingly, we understand more completely the human dynamics surrounding decisions and thus are able to dialogue more richly about issues. Meaningful dialogue such as this will have the dual benefit of advancing teamwork and facilitating decision-making.
Construction of new facilities in the healthcare arena is an ongoing, almost daily, occurrence. The desire to build wisely and effectively is evidenced at the Anne Arundel Medical Center which has ...attracted healthcare executives from all over the country who come to view, analyze, and experience the beauty, utility and interdependencies of the buildings and facilities that constitute "the AAMC campus." However, too often these executive visitors and benchmarking experts tend to focus on the technical, architectural, engineering, concrete aspects of the hospital, while naively overlooking and/or giving short shrift to the more critical behavioral dynamics of the construction process. The ultimate success of any building project requires a clear understanding by the leadership of "where people are coming from," so that both the design and the development of the final product can be brought under the synthesizing umbrella of patient care, clinical excellence, individual safety, and community responsibility. Not only must the leadership determine and drive the strategic thrust toward the final outcome; in addition, they must make sure that they allow significant colleagues to be actively, operationally, and symbolically engaged in a process that ends up in a structural outcome that everyone is proud to own, to see, and to inhabit.
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