In coffee (Coffea arabica L.), male sterility is a prerequisite for the exploitation of heterosis since it provides an efficient and reliable method for the production of hybrid seeds. Given its ...relevance, the objective of this study was to identify male-sterile genotypes within the Colombian Coffee Collection that can be used in genetic improvement. For this purpose, Ethiopian germplasm and progenies derived from hybrids between C. arabica x C. canephora were explored between 2017 and 2021. In the first stage, genotypes without visual presence of pollen were preselected in the field, followed by selection through staining and verification of male sterility and female fertility through directed crosses (directed, reciprocal and selfing). In this stage, 9,753 trees were explored, preselecting 2.4% due to visual absence of pollen. The staining of structures allowed us to confirm the lack or sporadic production of pollen in 23 individuals of Ethiopian origin. The results of the directed crosses led to the identification of 11 male-sterile and 12 partially male-sterile genotypes belonging to 15 accessions. In all cases, the individuals were characterized by the presence of anthers but with an absence or low content of pollen, which is why the male sterility is possibly of the sporogenic type. The female receptivity values were between 2.9% and 72.6%, being higher than 30% in five genotypes. These genotypes are a valuable tool for the genetic improvement of C. arabica with the potential to facilitate the use of heterosis and to allow a deeper understanding the development of male gametophytes in the species.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
2.
State Taxes and Spatial Misallocation FAJGELBAUM, PABLO D.; MORALES, EDUARDO; SERRATO, JUAN CARLOS SUÁREZ ...
The Review of economic studies,
01/2019, Letnik:
86, Številka:
1 (306)
Journal Article
Recenzirano
Odprti dostop
We study state taxes as a potential source of spatial misallocation in the U.S.. We build a spatial general equilibrium framework that incorporates salient features of the U.S. state tax system, and ...use changes in state tax rates between 1980 and 2010 to estimate the model parameters that determine how worker and firm location respond to changes in state taxes. We find that heterogeneity in state tax rates leads to aggregate welfare losses. In terms of consumption equivalent units, harmonizing state taxes increases worker welfare by 0.6% if government spending is held constant, and by 1.2% if government spending responds endogenously. Harmonization of state taxes within Census regions achieves most of these gains. We also use our model to study the general equilibrium effects of recently implemented and proposed tax reforms.
Distance metric learning is a branch of machine learning that aims to learn distances from the data, which enhances the performance of similarity-based algorithms. This tutorial provides a ...theoretical background and foundations on this topic and a comprehensive experimental analysis of the most-known algorithms. We start by describing the distance metric learning problem and its main mathematical foundations, divided into three main blocks: convex analysis, matrix analysis and information theory. Then, we will describe a representative set of the most popular distance metric learning methods used in classification. All the algorithms studied in this paper will be evaluated with exhaustive testing in order to analyze their capabilities in standard classification problems, particularly considering dimensionality reduction and kernelization. The results, verified by Bayesian statistical tests, highlight a set of outstanding algorithms. Finally, we will discuss several potential future prospects and challenges in this field. This tutorial will serve as a starting point in the domain of distance metric learning from both a theoretical and practical perspective.
This paper studies the influence of community capitals on well-being through a Community Capital Index (CCI) within coffee-growing families in southern Colombia. Our results show different farm ...typologies, with different levels of capital endowment translated into well-being that, in our case, were represented in the CCI. Specifically, social and political capitals positively affect coffee-growing families' decisions in terms of life strategies. The results of this study increase our understanding of welfare enhancement and its relationship with capital endowment according to the type of coffee producer, having implications for the planning of more effective programs towards the improvement of quality of life.
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Arbuscular mycorrhizal fungi (AMF) are the most prominent mycobionts of plants in the tropics, yet little is known about their diversity, species compositions and factors driving AMF distribution ...patterns. To investigate whether elevation and associated vegetation type affect species composition, we sampled 646 mycorrhizal samples in locations between 1000 and 4000 m above sea level (masl) in the South of Ecuador. We estimated diversity, distribution and species compositions of AMF by cloning and Sanger sequencing the 18S rDNA (the section between AML1 and AML2) and subsequent derivation of fungal OTUs based on 99% sequence similarity. In addition, we analyzed the phylogenetic structure of the sites by computing the mean pairwise distance (MPD) and the mean nearest taxon difference (MNTD) for each elevation level. It revealed that AMF species compositions at 1000 and 2000 masl differ from 3000 and 4000 masl. Lower elevations (1000 and 2000 masl) were dominated by members of Glomeraceae, whereas Acaulosporaceae were more abundant in higher elevations (3000 and 4000 masl). Ordination of OTUs with respect to study sites revealed a correlation to elevation with a continuous turnover of species from lower to higher elevations. Most of the abundant OTUs are not endemic to South Ecuador. We also found a high proportion of rare OTUs at all elevations: 79-85% of OTUs occurred in less than 5% of the samples. Phylogenetic community analysis indicated clustering and evenness for most elevation levels indicating that both, stochastic processes and habitat filtering are driving factors of AMF community compositions.
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•Overview of ketonization of carboxylic acids, aldehydes, alcohols, and esters.•Acid, aldehyde, alcohol, and ester ketonization pathways are closely related.•Ketonization depends on ...catalyst redox and acid-base properties and pretreatment.•Additional kinetic, spectroscopic, and computational studies further required.
The ketonization of various renewable biomass-derived oxygenated feedstocks such as carboxylic acids, aldehydes, alcohols, and esters is presented. As most previous ketonization reports focused on carboxylic acids, this overview extends the scope to homo-, cross-, and cyclo-ketonization of not only carboxylic acids (e.g., acetic, propionic, butyric, lactic, valeric, levulinic, dicarboxylic, and higher acids), but also of lesser reported aldehydes (e.g., acetaldehyde, propionaldehyde, and butyraldehyde), alcohols (e.g., ethanol, propanol, butanol, diols, and higher alcohols), and esters (e.g., small, intermediate, and large alkanoate chain length esters) aiming at obtaining more stable products, biofuels, and high-value chemicals. Ketonization of carboxylic acids (e.g., catalysts, mechanisms) is presented to provide the basis for understanding the ketonization of aldehydes, alcohols, and esters over metal oxides including ceria, titania, and zirconia, which have been the most active and studied catalysts for this reaction. Whenever possible, examples of ketonization of biomass-derived oxygenated compounds are presented to highlight the utility of the reaction and its practical application. As biomass product streams can contain a large number of different oxygenated compounds (e.g., more than 350 compounds in bio-oil), ketonization examples in the presence of more than one oxygenated molecule have also been considered.
Airborne laser scanning (ALS) can be utilised to derive canopy height models (CHMs) for individual tree crown (ITC) delineation. In the case of forest areas subject to defoliation and dieback as a ...result of disease, increased irregularities across the canopy can add complications to the segmentation of ITCs. Research has yet to address this issue in order to suggest appropriate techniques to apply under conditions of forest stands that are infected by phytopathogens. This study aimed to find the best method of ITC delineation for larch canopies affected by defoliation as a result of a Phytophthora ramorum infection. Sample plots from two study sites in Wales, United Kingdom, were selected for ITC segmentation assessment across a range of infection levels and stand characteristics. The performance of two segmentation algorithms (marker-controlled watershed and region growing) were tested for a series of CHMs generated by a standard normalised digital surface model and a pit-free algorithm, across a range of spatial resolutions (0.15 m, 0.25 m and 0.5 m). The results show that the application of a pit-free CHM generation method produced improved segmentation accuracies in moderately and heavily infected larch forest, compared to the standard CHM. The success of ITC delineations was also influenced by CHM resolution. Across all plots the CHMs with a 0.25 m pixel size performed consistently well. However, lower and higher CHM resolutions also provided improved delineation accuracies in plots dominated by larger and smaller canopies respectively. The selected segmentation method also influenced the success of ITC delineations, with the marker-controlled watershed algorithm generating significantly more accurate results than the region growing algorithm (p < 0.10). The results demonstrate that ITCs in forest stands infected with Phytophthora ramorum can be successfully delineated from ALS when a pit-free algorithm is applied to CHM generation.
Cacao (Theobroma cacao L.) has traditionally been considered a crop that requires shade, and consequently it is implemented under agroforestry systems (AFs) in order to regulate the level of incident ...solar radiation. However, optimal shade levels for this tree crop may vary depending on the climate conditions of where it is grown. Here we analyzed the physiological performance of cacao under three different AFs in the Colombian Amazon that differed in solar radiation patterns: high (HPAR), medium (MPAR), or low (LPAR) mean daily incident radiation. The physiological performance was characterized using photosynthetic variables in leaves such as light- and CO2-response curves, chlorophyll a fluorescence parameters, and total chlorophyll and carotenoid contents, in conjunction with other leaf functional traits. Cacao trees exposed to HPAR showed an improved physiological performance as compared to those from the other two AFs. Compared to MPAR and LPAR, cacao trees in HPAR doubled the rate of net carbon assimilation and reached higher maximum rates of RuBisCO carboxylation and RuBP regeneration. Moreover, cacao trees in HPAR presented photoprotection mechanisms that avoided photoinhibition, which was accompanied by a greater non-photochemical quenching coefficient and biochemical and morphological adjustments (low chlorophyll but higher carotenoid contents and low specific leaf area) compared to cacao trees from the other AFs. Overall, our results show that, due to the high cloud cover in the Colombian Amazon, cacao plantations under conditions of sparse shade maximized their carbon use, showing an improved physiological performance as a result of higher photosynthetic rates and energy dissipation mechanisms. If the crop were managed with sparse shade, the paradigm that favors the cultivation of cacao under shade would be called into question in the Colombian Amazon and other regions with similar climatic conditions.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
To study the differences in the levels of nitrogen metabolites, such as ammonia and nitric oxide and the correlations existing among them in both red blood cells (RBCs) and serum, as well as the ...possible differences by gender in healthy subjects and patients with type 2 Diabetes Mellitus (DM).
This cross-sectional study included 80 patients diagnosed with type 2 DM (40 female and 40 male patients) and their corresponding controls paired by gender (40 female and 40 male). We separated serum and RBC and determined metabolites mainly through colorimetric and spectrophotometric assays. We evaluated changes in the levels of the main catabolic by-products of blood nitrogen metabolism, nitric oxide (NO), and malondialdehyde (MDA).
Healthy female and male controls showed a differential distribution of blood metabolites involved in NO metabolism and arginine metabolism for the ornithine and urea formation. Patients with DM had increased ammonia, citrulline, urea, uric acid, and ornithine, mainly in the RBCs, whereas the level of arginine was significantly lower in men with type 2 DM. These findings were associated with hyperglycemia, glycosylated hemoglobin (Hb A1C), and levels of RBC's MDA. Furthermore, most of the DM-induced alterations in nitrogen-related metabolites appear to be associated with a difference in the RBC capacity for the release of these metabolites, thereby causing an abrogation of the gender-related differential management of nitrogen metabolites in healthy subjects.
We found evidence of a putative role of RBC as an extra-hepatic mechanism for controlling serum levels of nitrogen-related metabolites, which differs according to gender in healthy subjects. Type 2 DM promotes higher ammonia, citrulline, and MDA blood levels, which culminate in a loss of the differential management of nitrogen-related metabolites seen in healthy women and men.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Aims
To analyse the histological structure and histomorphometric characteristics of human hard palatal mucosa in order to determine the donor site of choice for connective tissue grafts from a ...histological point of view.
Materials and Methods
Palatal mucosa samples from six cadaver heads were harvested at four sites: incisal, premolar, molar and tuberosity. Histological and immunohistochemical techniques were performed, as was histomorphometric analysis.
Results
In the current study, we found that the density and size of cells were higher in the superficial papillary layer, whereas the thickness of the collagen bundles increased in the reticular layer. Excluding the epithelium, the mean percentage of lamina propria (LP) and submucosa (SM) was 37% and 63%, respectively (p < .001). LP thickness showed similar values in the incisal, premolar and molar regions, and a significantly greater thickness in tuberosity (p < .001). The thickness of SM increased from incisal to premolar and molar, disappearing in the tuberosity (p < .001).
Conclusions
As dense connective tissue of LP is the tissue of choice for connective tissue grafts, the best donor site from a histological point of view is tuberosity because it is composed only of a thick LP without the presence of a loose submucosal layer.
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