The effect of e-glass fiber volumetric on transverse strength of an acrylic resin denture plate repair. Acrylic resin is the most commonly material for the denture base. A disadvantage of acrylic ...resin is that it is easily to be cracked. One of the ways to resolve this problem is by adding the E-glass fibers. The purpose of this research was to find out the effect of volumetric E-glass fiber on transverse strength of an acrylic resin denture plate repair. The experiment involved thirty plates of heat cured acrylic with the dimensions of 65 × 10 × 2.5 mm. The specimens were prepared to create a 3-mm gap and 45° bevel. Subjects were divided in to 3 groups, each of which contained 10. Group I (control) was with no fiber reinforcement, group II was reinforced with 3.7vol % E-glass fiber, and group III was reinforced with 7.4 volume % E-glass fiber. All plates were soaked in distillation water for one day at 37 °C. Plates were tested for transverse strength with Universal Testing Machine and all data obtained was analyzed with one way anova at 95% confidence level (α= 0.05). The significant difference was found between the transversal force of acrylic resin plat enforced with fiber and other group without being reinforced with fibers (p<0.05). Group reinforced with 7.4 vol % E-glass fibers showed a significant difference (higher) than the group reinforced with 3.7 volume % fibers. The addition of E-glass fibers in an acrylic resin plate repair material increased the transverse strength. The increase in volumetric fibers might improve the transverse strength of an acrylic resin plate repair material.ABSTRAKResin akrilik merupakan bahan yang sering digunakan dalam pembuatan basis gigi tiruan. Kekurangan dari bahan resin akrilik adalah mudah patah. Salah satu cara yang dapat digunakan untuk mengatasi masalah tersebut adalah dengan menambahkan E-glass fiber. Tujuan untuk mengetahui pengaruh volumetrik E-glass fiber terhadap kekuatan transversal reparasi plat gigi tiruan resin akrilik. Penelitian ini menggunakan tiga puluh plat resin akrilik kuring panas dengan ukuran 65 × 10 × 2,5 mm. Spesimen dipreparasi untuk membentuk jarak 3 mm dan sudut bevel 45°. Subjek kemudian dibagi menjadi 3 kelompok, setiap kelompok terdiri dari 10 plat. Kelompok I (kontrol) tanpa diberikan penguat fiber, kelompok II diperkuat dengan 3,7 vol % E-glass ber, dan kelompok III diperkuat dengan 7,4 vol % E-glass fiber. Seluruh plat kemudian direndam dalam air destilasi selama satu hari pada suhu 37 °C. Plat resin akrilik kemudian diuji menggunakan Universal Testing Machine untuk mengetahui kekuatan transversal dan data yang didapatkan dianalisis menggunakan Anova satu jalur dengan tingkat kepercayaan 95% (α=0,05). Hasil menunjukkan terdapat perbedaan signifikan antara kekuatan transversal plat resin akrilik yang diperkuat dengan fiber dengan kelompok tanpa diperkuat fiber (p < 0,05). Kelompok yang diperkuat dengan 7,4 vol % E-glass fiber menunjukkan perbedaan signi kan (lebih tinggi) dibandingkan kelompok yang diperkuat dengan 3,7 vol % fiber. Kesimpulan bahwa peningkatan volume dari E-glass fiber dapat meningkatkan kekuatan transversal reparasi plat gigi tiruan resin akrilik.
BACKGROUND AND AIMS OF THE WORK: Nitric oxide (NO) has been reported to enhance the production of cAMP by hydroxyapatite (HA)-induced a human osteoblast cell line (HOS cells). The aim of the present ...study was to test the hypothesis that exogenous NO may up-regulate the proliferation of hydroxyapatite (HA)-induced HOS cells via the cyclic-AMP-protein kinase A (PKA) pathway.
HOS cells were pre-incubated with ODQ (guanylyl cyclase inhibitor), SQ22536 (adenylyl cyclase inhibitor), forskolin (adenylyl cyclase activator), IBMX phosphodiesterase (PDE) inhibitor, siguazodan (PDE3 inhibitor), rolipram (PDE4 inhibitor), or KT5720 (PKA inhibitor), and then, cultured on the surface of HA with or without the presence of SNAP (NO donor). The HOS cell cultures on the HA surface were added with br-cGMP (cGMP analogue), db-cAMP (cAMP analogue) with or without SNAP. The cell proliferation was assessed by a colorimetric assay.
The up-regulatory effect of SNAP on HA-induced HOS cell proliferation was suppressed by SQ22536 and KT5720, but enhanced by db-cAMP, IBMX, and rolipram. The HA-induced HOS cell proliferation with or without the presence of SNAP was unaltered by ODQ br-cGMP and siguazodan.
These results suggest, therefore, that HA-induced HOS cell proliferation may be mediated by the cAMP-PKA pathway regulated by PDE4 and that exogenous NO may amplify this cyclic nucleotide pathway, thereby augmenting HA-induced HOS cell proliferation.
The aim of the present study was to examine the osseointegration in low-density bone tissue for SLAffinity-treated implants with StemBios (SB) cell therapy.
The morphologies of SLAffinity-treated ...surfaces were characterized using scanning electron microscopy. In the animal model, implants were installed in the mandibular canine-premolar area of 12 miniature pigs. Each pig received 3 implants of machine, sand blasted, large grit, and acid etched, and SLAffinity-treated implants. In the clinical trial, 10 patients received 1 SLAffinity-treated implant in the maxilla in the posterior area and 1 patient with low bone tissue density received 2 SLAffinity-treated implants with SB cell therapy. Resonance frequency analysis and computed tomography were assessed monthly over the first 3 months after implant placement.
The results demonstrated that surface treatment significantly affected early osseointegration in patients who received SB cell therapy. SB cell therapy transferred the stress caused by the implant more uniformly, and the stress decreased with healing time. SLAffinity-treated implants also proved clinically successful after the 3 months.
The SLAffinity treatments enhanced osseointegration significantly, especially at early stages of bone tissue healing with SB cell therapy.
Denture wearing is very important after tooth extraction, because it can rehabilitate-mastication, phonetics and aesthetics. After tooth-extraction, alveolar bone resorption disturbs the stability ...and retention of denture. Hydroxyapatite has several weaknesses and high bone density. Stichopus hermani collagen is a product of gold sea cucumber collagen extraction which contains 80% collagen. The purpose was to observe the effect of Stichopus hermanni collagen with local hydroxyapatite as bone substitute material toward osteoclast number and toxicity.The subjects were 75 male of Rattus Sprague Dawley, each was 3 months old. The subjects were divided into 3 groups. Group I was Stichopus hermanni collagen and local hydroxyapatite, group II was Stichopus hermanni collagen, and group III was collagen. Each of the subject was decapitated after 3, 7, 14, 28, 56 days after treatment. The defect was made on the femur condyle of Rattus Sprague Dawley. The histology slides were made from defect area. The trinocular microscope was used to measure osteoclast. The data were analyzed using the two-ways ANOVA test. The tests of hepatic and renal toxicity were done by making histology slides measured with trinocular microscope. The data were analyzed using Kruskal Wallis test. Result: The two-ways ANOVA test showed there was significant difference among group of Stichopus hermanni collagen and local hydroxyapatite, group of Stichopus hermanni collagen, and group of collagen after 3, 7, 14, 28, 56 days of treatment on the number of osteoclast (p<0.05). The Kruskal Wallis test showed there was no significant difference between group of Stichopus hermanni collagen and local hydroxyapatite, and collagen after 3, 7, 14, 28, 56 days of treatment on the hepatic and renal toxicity (p>0.05). Conclusion: Stichopus hermanni collagen with local hydroxyapatite as bone substitute material increases osteoclast compared with Stichopus hermanni collagen, and collagen. Stichopus hermanni collagen with local hydroxyapatite does not cause systemic toxicity.
The aim of the present study was to test the hypothesis that the proliferation of a human osteoblast cell line (HOS cells) stimulated with hydroxyapatite (HA) may be regulated by nitric oxide (NO). ...The cells were cultured on the surface of HA. Medium or cells alone were used as controls. L-arginine, D-arginine, 7-NI (an nNOS inhibitor), L-NIL (an iNOS inhibitor), L-NIO (an eNOS inhibitor) or carboxy PTIO, a NO scavenger, was added in the HA-exposed cell cultures. The cells were also precoated with anti-human integrin alphaV antibody. The levels of nitrite were determined spectrophotometrically. Cell proliferation was assessed by colorimetric assay. The results showed increased nitrite production and cell proliferation by HA-stimulated HOS cells up to day 3 of cultures. Anti-integrin alphaV antibody, L-NIO, or carboxy PTIO suppressed, but L-arginine enhanced, nitrite production and cell proliferation of HA-stimulated HOS cells. The results of the present study suggest, therefore, that interaction between HA and HOS cell surface integrin alphaV molecule may activate eNOS to catalyze NO production which, in turn, may regulate the cell proliferation in an autocrine fashion.
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