Objective. To evaluate the Fas‐dependent signaling pathway, we examined the involvement of protein tyrosine phosphorylation and the DNA binding activity of AP‐1 in rheumatoid arthritis (RA) cultured ...synovial cells.
Methods. The number of dead cells was counted after treatment with anti‐Fas antibody in the presence of protein tyrosine kinase or phosphatase inhibitor. Protein tyrosine phosphorylation in synoviocytes after Fas ligation was examined by immunoblot and immunoprecipitation analyses. The DNA binding activity of AP‐1 was examined by electrophoretic mobility shift assay.
Results. Treatment with the protein tyrosine phosphatase inhibitor, orthovanadate, significantly enhanced the apoptosis of RA synoviocytes after Fas ligation. Ligation of the Fas molecule on RA synoviocytes induced a rapid tyrosine phosphorylation of JNK (c‐Jun amino‐terminal kinase) and formation of the AP‐1 transcription factor.
Conclusion. Our results strongly suggest that the JNK/AP‐1 signaling pathway is activated during the process of Fas‐mediated apoptosis of RA synovial cells.
A 28-year-old woman had persistent pain of both hip joints since the age of 13 years. X-ray analysis showed destructive changes in both hip joints and ossification of sacroiliitic joints. The patient ...had mild diarrhea and slight abdominal pain for 8 years. Blood-stained stool was not noticed. Barium enema showed changes consistent with the diagnosis of ulcerative colitis (UC). Inflammatory bowel syndrome should be considered in patients with persistent coxitis, even in the absence of severe abdominal symptoms.
Experimental study of the decay K L 0 → π 0 ν ν Ahn, J. K.; Akune, Y.; Baranov, V. ...
Physical review. D, Particles, fields, gravitation, and cosmology,
4/2010, Letnik:
81, Številka:
7
Journal Article
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