CArG binding factor A (CBF-A) is a transcription factor first isolated from mouse C2 myogenic cells. Several lines of evidence indicate that CBF-A is also present in the anterior pituitary lobe and ...participates in the process of development and cell transformation. This study was performed to clone porcine CBF-A and to investigate its roles in the porcine anterior pituitary lobe. The predicted amino acid sequence of porcine CBF-A showed a unique insertion of five TG-repeats in the N-terminal region in comparison with those of other mammals, whereas the other regions appeared to be mostly conserved including two RNA recognition motifs in the middle region. Investigation of the expression of CBF-A gene during porcine pituitary development by RT-PCR showed an exclusive and temporary decrease in expression level shortly after birth in both sexes that was gradually but insufficiently restored. The expression of fluorescence protein-fused CBF-A in CHO cells demonstrated that CBF-A is located in the nuclei. We examined whether CBF-A regulates the expression of pituitary hormone genes in CHO cells and found that CBF-A significantly stimulated the promoter activity of growth hormone and prolactin by about 2-fold but did not stimulate the LHbeta gene. The specific DNA binding ability of porcine CBF-A was examined using serial oligonucleotides, CArG box and CC(W)sub(0-6)GG (W=A or T). As a result, porcine CBF-A was shown to have a high binding affinity for double- and single-stranded CC(W)sub(6)GG but no affinity for the known sequences of the CBF-A-target genes. Accordingly, this study demonstrated that porcine CBF-A may play a role in regulating at least two pituitary hormone genes, GH and PRL.
ABSTRACT
BACKGROUND
The sitting cervical position for elective posterior cervical decompression and fusion affords advantages over prone positioning, but remains unpopular due to concerns about ...venous air embolism (VAE).
OBJECTIVE
To demonstrate the safety and efficacy of sitting cervical surgery in our series and in the literature.
METHODS
To evaluate the incidence of complications, we retrospectively identified all adult patients who underwent elective sitting posterior cervical surgeries at our institution from 2009 to 2014. Using International Classification of Diseases-9 coding, we searched for incidences of air embolism, myocardial infarction, pulmonary embolism, and deep vein thrombosis. Operative time, estimated blood loss, and case type distribution were also recorded. We then calculated the incidence of clinically significant VAE in sitting cervical surgeries.
RESULTS
Between 2009 and 2014, 558 surgeries were performed in the sitting cervical position. No VAE was identified. The average operative time was 1 h 25 min. A total of 30-d perioperative complications among sitting position patients included 3 myocardial infarctions, 1 pulmonary embolism attributed to venous thrombosis, and 2 patients with deep venous thrombosis for a total cardiovascular complication rate of 1.1%. The incidence of clinically significant VAE in the literature is 1.5%.
CONCLUSION
We present the second largest case series to date on the sitting position for cervical surgeries, highlighting its safety and efficacy. This position provides a surgical field with superior visualization, allowing decreased operative time and blood loss. The risk of clinically significant VAE is low. Intraoperative monitoring for these events with less invasive means is safe and effective.
Production of the K*(892)0 and K̄*(892)⁰ resonances was studied via their K⁺π⁻ and K⁻π⁺ decay modes in central Pb+Pb, Si+Si, C+C, and inelastic p+p collisions at 158A GeV(√(sNN)=17.3 GeV) with the ...NA49 detector at the CERN SPS. Transverse momentum and rapidity distributions were measured and total yields were estimated. The yield of K* exceeds that of K̄* by about a factor of two in nucleus-nucleus reactions. The total yield ratios ⟨K*⟩/⟨K+⟩ and ⟨K̄*⟩/⟨K-⟩ are strongly suppressed in central Pb+Pb compared to p+p, C+C, and Si+Si collisions, in agreement with the expected attenuation of these short-lived resonance states in the hadronic phase of the expanding fireball. The UrQMD model, although incorporating such a scenario, does not provide a quantitative description of the experimental results. The statistical hadron gas model assuming the same freeze-out parameters for stable hadrons and resonances overestimates the ⟨K*⟩/⟨K⟩ ratios in central Pb+Pb collisions by about a factor of 2.5.
Abstract The production of $$K^{0}_{S}$$ K S 0 mesons in inelastic p+p collisions at beam momentum 158 $$\text{ GeV }/c$$ GeV / c ( $$\sqrt{s_{NN}}=17.3$$ s NN = 17.3 $$\text{ GeV }$$ GeV ) was ...measured with the NA61/SHINE spectrometer at the CERN Super Proton Synchrotron. Double-differential distributions were obtained in transverse momentum and rapidity. The mean multiplicity of $$K^{0}_{S}$$ K S 0 was determined to be $$0.162 \pm 0.001 (stat.) \pm 0.011 (sys.)$$ 0.162 ± 0.001 ( s t a t . ) ± 0.011 ( s y s . ) . The results on $$K^{0}_{S}$$ K S 0 production are compared with model predictions (EPOS 1.99, SMASH 2.0, PHSD and UrQMD 3.4 models) as well as with published world data.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Abstract Double-differential yields of $${\Xi \left( 1530\right) ^{0}} $$ Ξ 1530 0 and $${\overline{\Xi }\left( 1530\right) ^{0}} $$ Ξ ¯ 1530 0 resonances produced in p+p interactions were measured ...at a laboratory beam momentum of 158 $$\text{ GeV }\!/\!c$$ GeV / c . This measurement is the first of its kind in p+p interactions below LHC energies. It was performed at the CERN SPS by the NA61/SHINE collaboration. Double-differential distributions in rapidity and transverse momentum were obtained from a sample of $$26\times 10^6$$ 26 × 10 6 inelastic events. The spectra are extrapolated to full phase space resulting in mean multiplicity of $${\Xi \left( 1530\right) ^{0}} $$ Ξ 1530 0 ( $$6.73 \pm 0.25\pm 0.67)\times 10^{-4}$$ 6.73 ± 0.25 ± 0.67 ) × 10 - 4 and $${\overline{\Xi }\left( 1530\right) ^{0}} $$ Ξ ¯ 1530 0 ( $$2.71 \pm 0.18\pm 0.18)\times 10^{-4}$$ 2.71 ± 0.18 ± 0.18 ) × 10 - 4 . The rapidity and transverse momentum spectra and mean multiplicities were compared to predictions of string-hadronic and statistical model calculations.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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