We reported recently that a pituitary‐specific transcription factor PROP1 is present in SOX2‐positive cells and disappears at the early stage of the transition from progenitor cell to committed cell ...during the embryonic development of the rat pituitary. In the present study, we examined the localisation and identification of SOX2‐positive and PROP1/SOX2‐positive cells in the neonatal and postnatal rat pituitaries by immunohistochemistry. Quantitative analysis of immunoreactive cells demonstrated that SOX2‐positive pituitary stem/progenitor cells are not only predominantly localised in the marginal cell layer, but also are scattered in the parenchyma of the adult anterior lobe. In the marginal cell layer, the number of PROP1/SOX2‐positive cells significantly decreased after postnatal day 15, indicating that a significant quantitative transition is triggered in the marginal cell layer during the first postnatal growth wave of the anterior pituitary. By contrast, other phenotypes of SOX2‐positive stem/progenitor cells that express S100β appeared in the postnatal anterior pituitary. These data suggested that quantitative and qualitative transition occurs by acquisition of a novel mechanism in terminal differentiation in the postnatal development of the anterior pituitary.
We recently cloned a paired‐related homeodomain protein Prx2 as a novel factor in the pituitary. In the present study, we investigated the ontogenic profiles of Prx2 and another cognate Prx1 in the ...rat embryonic pituitary. Quantitative real‐time polymerase chain reaction showed low expression of Prx2 and a marked increase of Prx1 on rat embryonic day (E)20.5. Immunohistochemical analyses using an antibody that recognises both proteins, with the aim of investigating their roles in pituitary organogenesis, demonstrated that PRXs first appear in the Rathke’s pouch on E13.5 in the pituitary stem/progenitor cells expressing Prop1 and Sox2. After E16.5, the number of Prx‐expressing cells was increased in both anterior and intermediate lobes. SOX2+ stem/progenitor cells in the intermediate lobe started to produce PRXs, and PRX+/SOX2+/PROP1+‐cells were present on the anterior side of the marginal cell layer and were scattered in the parenchyma of the anterior lobe. On the other hand, PRX+‐cells negative for PROP1 and SOX2 were located in the anterior lobe. Analysis of the relationship with pituitary endocrine cells revealed that a part of PRX+/PROP1−/SOX2−‐cells in the anterior lobe co‐expressed all types of hormones. The proportion of co‐localisation of PRXs and hormones was highest on the day each hormone first appeared. These data indicate that PRXs are produced in the pituitary progenitor cells and may play roles in the process of terminal differentiation during early pituitary organogenesis. An in vitro small interfering RNA‐knockdown experiment in the pituitary‐derived cell line, TtT/GF, revealed that PRX1 and PRX2 play roles in proliferation by different mechanisms because knockdown of Prx2, but not Prx1, induced the p21 expression. Furthermore, immunohistochemical analysis demonstrated that 76% of PRXs+ cells were positive for a cell proliferation marker Ki67 in the E18.5 pituitary. This is the first report of the involvement of PRX1 and PRX2 in organogenesis of tissue originating from the ectoderm other than the mesoderm.
New data on the production of charged kaons in p+p interactions are presented. The data come from a sample of 4.8 million inelastic events obtained with the NA49 detector at the CERN SPS at 158 GeV/c ...beam momentum. The kaons are identified by energy loss in a large TPC tracking system. Inclusive invariant cross sections are obtained in intervals from 0 to 1.7 GeV/c in transverse momentum and from 0 to 0.5 in Feynman x. Using these data as a reference, a new evaluation of the energy dependence of kaon production, including neutral kaons, is conducted over a range from 3 GeV to
collider energies.
Intra-arterial tissue plasminogen activator (IA tPA) is sometimes used in conjunction with aspiration catheters and stentrievers to achieve recanalization in endovascular thrombectomy (ET) for large ...vessel occlusion (LVO). Reports of safety and efficacy of this approach are limited by technical heterogeneity and sample size.
We retrospectively reviewed a data set of patients undergoing ET for LVO between August 2017 and September 2020 to identify those that received IA tPA. IA tPA usage, timing and dosage was at the discretion of the operative neurosurgeon. We identified three broad categories of IA tPA administration: (1) adjunctive with the first pass; (2) salvage with subsequent passes after first pass achieved incomplete revascularization; and (3) post-thrombectomy residual distal occlusions. Univariate and multivariate logistic regression were performed to test associations with recanalization, hemorrhage, and functional independence.
Among 271 patients, 158 (58%) patients had IA tPA, of which 83 received adjuvant IA tPA, 60 received salvage IA tPA, and 15 received post-thrombectomy IA tPA for distal occlusions. There were no differences in demographics, stroke etiology and premorbid medications between these groups. Patients receiving salvage IA tPA had longer times from groin access to recanalization and more passes, as expected. On multivariate analysis neither adjunctive nor salvage IA tPA was significantly associated with recanalization, post-operative hemorrhage, or functional outcomes. On univariate analysis, patients receiving salvage IA tPA had lower rates of TICI 3 or 2b revascularization (80% vs. 89% adjunctive and 92% no IA tPA, p = 0.003) and higher rates of any postoperative hemorrhage (33% vs. 22% adjunctive and 19% no IA tPA, p = 0.003).
In this retrospective, single-institution series, IA tPA used adjunctively or as salvage therapy in ET for LVO was not associated with recanalization, post-operative hemorrhage, or functional outcomes, suggesting IA tPA is an available modality that can be utilized in cases of recalcitrant clots.
Background: Dorsal column stimulation may be utilized to treat non-neuropathic pain attributed to glenohumeral arthritis.
Case Description: An 84-year-old female presented with right shoulder pain ...for 3 years. She was diagnosed with glenohumeral arthritis and a complete loss of the joint space. She was treated with a dorsal column stimulator, requiring the electrodes to be placed from the inferior aspect of C3 to the superior aspect of T1. Six weeks postoperatively, she reported >90% coverage of her shoulder pain, demonstrated increased right arm function, and a reduction in her use of narcotics.
Conclusion: Dorsal column stimulation of C3-T1 proved to be an effective alternative treatment for drug-resistant glenohumeral arthritis in an 84-year-old female with a complete loss of the joint space.
Prophet of PIT1 (PROP1) is a pituitary-specific factor and responsive gene for the combined pituitary hormone deficiency in
Ames dwarf mice and human patients. Our immunohistochemical studies ...demonstrated that PROP1 is consistently expressed in SOX2-expressing stem/progenitor cells in the rat pituitary from embryonic (E) to postnatal periods. At E13.5, all the cells in Rathke’s pouch, the primordium of the pituitary, express PROP1. Afterward, PROP1-positive cells localize along the marginal cell layer, a putative stem cell niche in the pituitary, and stratify in the parenchyma of the anterior pituitary. In the embryonic period, PROP1 coexists transiently with PIT1, which is the anterior pituitary-specific factor and is a target of PROP1, but not any hormones. Thus, the present results imply a regulatory role of PROP1 not only in pituitary organogenesis but also in conversion of PIT1-lineage cells.
New data on the production of protons, anti-protons and neutrons in p+p interactions are presented. The data come from a sample of 4.8 million inelastic events obtained with the NA49 detector at the ...CERN SPS at 158 GeV/c beam momentum. The charged baryons are identified by energy loss measurement in a large TPC tracking system. Neutrons are detected in a forward hadronic calorimeter. Inclusive invariant cross sections are obtained in intervals from 0 to 1.9 GeV/c (0 to 1.5 GeV/c) in transverse momentum and from −0.05 to 0.95 (−0.05 to 0.4) in Feynman
x
for protons (anti-protons), respectively.
p
T
integrated neutron cross sections are given in the interval from 0.1 to 0.9 in Feynman
x
. The data are compared to a wide sample of existing results in the SPS and ISR energy ranges as well as to proton and neutron measurements from HERA and RHIC.
In this paper, a noncontact sensor for the measurement of liquid concentration using a quartz oscillator is reported. An out-of-plane electric field with a planer sensing capacitor (SC) is used for ...sensing a liquid on a spacer. This sensor measures the changes in the capacitance of the SC as the changes in the frequency of the quartz oscillator. The capacitance is dependent on the relative permittivity and the conductivity of the liquid sample on the spacer. Therefore, it is possible to measure the concentration of the sample. The response is very stable because in this sensor, the SC is incorporated into the quartz oscillator. Because the quartz crystal resonator is not contact with liquid, the proposed sensor has a high quality factor (approximately 30,000). Moreover, this sensor can be used with a general IC and an electronic component, with a low cost. The proposed sensor can be expected to be used for various kinds of liquid sensing applications.
PDF
