A 13-year-old boy presented with a very unusual ependymoma extending extra-axially. Computed tomography demonstrated a tumor with a cyst and calcification adjacent to the dura and extending over the ...right occipital and parietal lobes. The cyst wall and solid tumor were enhanced postcontrast. Magnetic resonance imaging revealed that the solid tumor was isointense on T1-weighted images and a mixed iso- and high-intensity on T2-weighted images. The solid tumor and tissue surrounding the cyst were enhanced markedly by gadolinium-diethylenetriaminepenta-acetic acid. Sagittal and coronal images demonstrated a multilocular tumor shadow. Cerebral angiography demonstrated a tumor fed by a posterior branch of the right middle meningeal artery but no feeders from the internal carotid and vertebral arteries. The tumor was removed en bloc. The histological diagnosis was clear cell-type ependymoma.
Modes of higher energy levels (excited states) in self-assembled InAs/GaAs quantum dots are investigated by PL and PLE polarization measurements with the aid of theoretical calculation. It is shown ...that (1) there exists the excited states of not only holes but also electrons, (2) the theoretical and experimental polarization properties are well coincident with each other, and mode assignment is successfully carried out, and (3) some hidden peaks are found between apparent peaks, and the weak emissions of the hidden peaks may be due to the strong interaction with phonons.
Minimal inhibitory concentration of isoxazole to the growth of the strains isolated from sputa of pulmonary tuberculous patients treated with it was higher than those of other sulfonamides in the ...reported experiment. An experiment was done on the strains isolated from sputa of pulmonary tuberculous patients who were excreting tuberculous bacilli in spite of the treatment with specific sulfonamides for a long time. Media used were 1% Ogawa's egg slant and Kirchner's semi-liquid agar media with albumin at the rate of 10%. The cross resistance and the power to Myc, tuberculosis were compared between sulfonamides already used and those never used in a correlation diagram and a histogram of minimal inhibitory concentration to the growth of Myc, tuberculosis at 3 weeks culture. 14 strains isolated from the sputa of pulmonary tuberculous patients treated with sulfisoxazole, 7 strains isolated from those treated with sulfamonomethoxin, 3 strains isolated from those treated with sulfadimethoxin and, as a control, H37Rv were used. 6 sulfonamides were used, that is, sulfisoxazole, sulfamethizole, sulfamezole, sulfamonomethoxin, sulfadimethoxin and sulfamethomidin. 1) Sulfamonomethoxin was most powerful to strain treated with specific sulfonamides in the used 6 sulfonamides. 2) The cross resistance may exist between different sulfonamides on Myc, tuberculosis, Also the one-way cross resistance between isoxazole and diazin may exist, too.
studies were made about the influence of the inoculated quantity and the culture period of Myc. tuberculosis to minimal inhibitory concentration( M. I. C.) of capreomycin to the growth of Myc. ...tuberculosis. Media used were 1% Ogawa's egg slant and Kirchner's semi-liquid agar media with albumin (Eiken) in 10%. The condense water of 1% Ogawa's egg slant and the basal media of Kirchner's semi-liquid agar media were adjusted to pH 6.8 with HCl and NaOH. Capreomycin was added in the concentration 0, 2.5, 5, 10, 25 and 50mcg/ml, and mixed thoroughly. They were dispensed into test tubes in 5ml each. Kirchner' semi-liquid agar media was incubated at 37°C for 24 hours to confirm the sterility. Used strain were H37Rv and 2 strains isolated from the sputa of pulmonary tuberculous patients untreated. From the 3 weeks old cultures of these strains on 1% Ogawa's egg slant emulsions were made in a concentration of approximately 1mg/ml employing electric photometer and were diluted 10 times, respectively. The 0.1ml each of the emulsions was inoculated into the media. They were incubated at 37°C for 6 weeks and observed weekly from the 2nd to the 6th week. Following result were obtained. The inoculated quantity and the length of the culture period influenced on the activity of capreomycin on 1% Ogawan's egg slant, but not so in Kirchner's semi-liquid agar media. M. I. C. of capreomycin to Myc. tuberculosis paralleled the inoculated quantity on 1% Ogawa's egg slant. In Kirchner's semi-liquid agar media, it was high only when remarkably much quantity of strain was inoculated. M I. C. of capreomycin becomes high with the prolongation of the culture period unrelated with the inoculated puantity on 1% Ogawa's egg slant. It becomes high with the prolongation of the culture period only when remarkably much puantity of strains inoculated. On the 3rd week, M. I. C. of capreomycin to 10-3mg inoculated bacilli was 2.5mcg/ml at 3 strains in Kirchner's semi-liquid agar media. It was 5-10-25mcg ml on 1% Ogawa's egg slant. In other words, M. I. C. of capreomycin on 1% ogawa's egg slant is 2-10 times higher than that in Kirchner's semi-liquid agar media.
1% Ogawa's egg slant was used, and pH of its condense water was adjusted 6.8 with HCI and NaOH. Used strains were following 6: H37Rv, Aoyama-B, two strains isolated from sputa of a pulmonary ...tuberculosis patients never treated (with chemotherapic substances) and 2 strains from those treated cycloserine and other antituberculous drugs. After 3 weeks culture on 1% Ogawa's egg slant, each strain was diluted 10-2 times by turbidometry and O, 1ml of each emulsions was inoculated. The influences of Lots of cycloserine to its minimal inhibitory concentration to Myc. tuberculosis were compaired. The potency of cycloserine to Myc, tuberculosis was not influenced by the Lot, when the conditions of strains and the media were kept constant.
The influences of the quantity of inoculated bacilli and the culture period on the minimal inhibitory concentration of Cycloserine (CS) to mycobacterium tuberculosis were experimented. Strain used ...were H37Rv strain and five strains isolated from sputa of pulmonary tuberculosis patients never treated with CS previously. Media used were 1% Ogawa's egg slant and Kirchner's semi-liquid agar media. Following result were obtained. 1) The absolute inhibitory concentration of CS to mycobacterium tuberculosis was inclined to go up according to the increase of the quantity of inoculated bacilli. In some case the growth of colony of contrast media became insufficient when the quantity of inoculated bacilli was too little. So, it is considered that 10mg is the suitable of inoculated bacilli at the routine resistance test with both media. 2) The absolute inhibitory concentration of CS to mycobacterium tuberculosis goes up according as the culture period is postponed beyond 4 weeks on both media. Besides appear of natural resistant was inclined to observed. So it is considered that 3 weeks is the suitable culture period on the resistance test. 3) On both media, the absolute inhibitory concentration of CS to mycobacterium tuberculosis was 10-20mcg/ml in case that the quantity of inoculated bacilli was 10-3mg and the culture period was 3 weeks. 4) The result of the resistance test of CS changed gradually from imperfect resistance to perfect resistance according to the postponement of culture period.
This subject was experimented with H37Rv strain and Inouestrain isorated from sputum of pulmonary tuberculosis patient treated with Cycloserine (CS) previously. Strains were cultured on 1% Ogawa's ...egg slaut for 3 weeks. Following results were obtained. 1) The minimal inhibitory concentration of CS to H37Rv strain on the resistance media used immediately after adjustment was 10 mcg/ml when it was cultured for 3 weeks on 1% Ogawa's egg slaut. And it was also 10mcg/ml when it was cultured for 2 weeks on Kirchber's semi-liquid agar media. It was 20mcg/ml when it was cultured for 4 weeks on 1% Ogawa's egg slaut as well as when it was cultured for 3 weeks on Kirchner's semi-liquid agar media. 2) Both the case when the media was preserved in a refrigerator at 5°C and the case when it was incubated at 20°C, the anti-tuberculous strength of CS was inclined to fall according to the postponement of preservative period on both media. 3) When they are preserved in a refrigerator at 5°C; The inhibitory power of CS to mycobacterium tuberculosis was equal to the biginning with in 4 weeks after adjustment on 1% Ogawa's egg slaut and within 2 weeks on Kirchner's semi-liquid agar media. When they are incubated at 20°C; It was equal to the beginning with in 1 week on 1% Ogawa's egg Slaut. and with in 2 weeks on Kirchner's semi-liquid agar media.
The influence of preservation temperature and period of media used for drug sensitivity test on the minimal inhibitory concentration of D-Ethambutol to the growth of Myco. tuberculosis was ...investigated. Media used were 1% Ogawa's egg slant and Kirchner's semi-liquid agar media with 10%. albumin. pH of the former was adjusted to 6.8 by suitable combination of 4% H2SO4 and 10%. Na3CO3, and pH of the basal media of the later was adjusted to 6.8. The concentration of D-Ethambutol added to the media was 0, 1, 2.5 and 10 mcg/ml. The media were dispensed into test tubes each 5 ml. Kirchner's semi-liquid agar media was used after being stored in an incubator at 37°C for 24 hours in order to confirm the sterility. After preparation, the media were stored at 5°C, 20°C, 30°C and 37°C for 1, 2, '3, 4, 6 and 8 weeks before their use. Strains used were 1187Rv and a strain isolated from sputa of a pulmonary tuberculosis. patient previously untreated with antituberculous drugs. Strains were planted on 1% Ogawa's egg slant before each experiment, and always 3 weeks culture was used for the experiment.10-3 mg of the strains were inoculated on each media stored at different temperature for 0 to 8 weeks. The results obtained were summarized as follows: 1) On both media, the minimal inhibitory concent r a tion of D-Ethambutol on the growth. of Myco. tuberculosis became slightly higher in accordance with the increase of preservation temperature and the prolongation of the preservation period. This effect was more marked on Kirchner's semi-liquid agar media than on 1% Ogawa's egg slant. 2) If the 3 weeks culture was inoculated on the medium immediately after preparation, the minimal inhibitory concentration of D-Ethambutol to the growth of Myco. tuberculosis was 2.5 mcg/ml on 1% Ogawa's egg slant and 5 mcg/ml in Kirchner's semi-liquid agar media.
In pyrolysis of PTC barium titanate precursor synthesized by the oxalate method, growth and morphological change of the particles formed were studied. Throughout the process, an aggregate particle ...was observed to be generally composed of a large number of crystallite particles. In the calcination process up to 1000°C, formation of various kinds of phases (dehydrated oxalate, amorphous phase and titanate) and grain growth of the titanate were observed in sequence, but the unique morphology and the size (100-200μm) of the aggregate particles, including the precursor particles, remained unchanged.
Factors influencing the minimal inhibitory concentration (M.I.C.) of rifampicin for Myc. tuberculosis were examined. Used strains were H37Rv and a strain isolated from the sputa of a previously ...untreated pulmonary tuberculous patient. 1% Ogawa's egg media and Kirchner's semiliquid agar media with 10% albumin were used. Following results were obtained. 1) Influence of pH; on alkaline side, the M.I.C. was low with I% Ogawa's egg media and high with Kirchner's semi-liquid agar media. 2) On both media, the more the dose of inoculated bacilli, the higher the M.I.C. 3) The longer the inoculation period, the higher the M.I.C., on both media. 4) When the media are preserved in high temperature, the M.I.C. of rifampicin became higher in eary stage. The longer the preservation period, the higher the M.I.C. And the influence of the preservation temperature was more remarkable in I% Ogawa's egg media than in Kirchiner's semi-liquid agar media.
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