We use the constituent quark model to extract polarized parton distributions and finally polarized nucleon structure function.Due to limited experimental data which do not cover whole （x,Q 2 ） plane ...and to increase the reliability of the fitting,we employ the Jacobi orthogonal polynomials expansion.It will be possible to extract the polarized structure functions for Helium,using the convolution of the nucleon polarized structure functions with the light cone moment distribution.The results are in good agreement with available experimental data and some theoretical models.
The introduction of the lung allocation score has brought lung transplantation (LTX) of patients on extracorporeal membrane oxygenation (ECMO) bridge into the focus of interest. We reviewed our ...institutional experience with ECMO as a bridge to LTX.
Between 1998 and 2011, 38 patients (median age 30.1 years, range 13-66 years) underwent ECMO support with intention to bridge to primary LTX. The underlying diagnosis was cystic fibrosis (n=17), pulmonary hypertension (n=4), idiopathic pulmonary fibrosis (n=9), adult respiratory distress syndrome (n=4), hemosiderosis (n=1), bronchiolitis obliterans (n=1), sarcoidosis (n=1), and bronchiectasis (n=1). The type of extracorporeal bridge was venovenous (n=18), venoarterial (n=15), interventional lung assist (n=1), or a stepwise combination of them (n=4). The median bridging time was 5.5 days (range 1-63) days. The type of transplantation was double LTX (n=7), size-reduced double LTX (n=8), lobar LTX (n=16), split LTX (n=2), and lobar LTX after ex vivo lung perfusion (n=1).
Four patients died before transplantation. Thirty-four patients underwent LTX, of them eight patients died in the hospital after a median stay of 24.5 days (range 1-180 days). Twenty-six patients left the hospital and returned to normal life (median hospital stay=47.5 days; range 21-90 days). The 1-, 3-, and 5-year survival for all transplanted patients was 60%, 60%, and 48%, respectively. The 1-, 3-, and 5-year survival conditional on 3-month survival for patients bridged with ECMO to LTX (78%, 78%, and 63%) was not worse than for other LTX patients within the same period of time (90%, 80%, and 72%, respectively, P=0.09, 0.505, and 0.344).
Transplantation of patients bridged on ECMO to LTX is feasible and results in acceptable outcome.
We examined the effect of alemtuzumab and basiliximab induction therapy on patient survival and freedom from bronchiolitis obliterans syndrome (BOS) in double lung transplantation. The United Network ...for Organ Sharing database was reviewed for adult double lung transplant recipients from 2006 to 2013. The primary outcome was risk‐adjusted all‐cause mortality. Secondary outcomes included time to BOS. There were 6117 patients were identified, of whom 738 received alemtuzumab, 2804 received basiliximab, and 2575 received no induction. Alemtuzumab recipients had higher lung allocation scores compared with basiliximab and no‐induction recipients (41.4 versus 37.9 versus 40.7, p < 0.001) and were more likely to require mechanical ventilation before to transplantation (21.7% versus 6.5% versus 6.2%, p < 0.001). Median survival was longer for alemtuzumab and basiliximab recipients compared with patients who received no induction (2321 versus 2352 versus 1967 days, p = 0.001). Alemtuzumab (hazard ratio 0.80, 95% confidence interval 0.67–0.95, p = 0.009) and basiliximab induction (0.88, 0.80–0.98, p = 0.015) were independently associated with survival on multivariate analysis. At 5 years, alemtuzumab recipients had a lower incidence of BOS (22.7% versus 55.4 versus 55.9%), and its use was independently associated with lower risk of developing BOS on multivariate analysis. While both induction therapies were associated with improved survival, patients who received alemtuzumab had greater median freedom from BOS.
While the use of either alemtuzumab or basiliximab induction confer a survival benefit over no induction therapy, the use of alemtuzumab prolongs time to bronchiolitis obliterans syndrome in double lung transplant recipients. Corris's editorial is on page 2250.
In the present study, a new design of collecting electrodes is studied for performing a higher performance in EHD phenomena in a smooth channel. The effect of the electric field on the heat transfer ...enhancement and swirling flow patterns for a new design of multiple collectors in a smooth channel is numerically investigated with 2-D approach. In this regard, the corona device configuration comprises a single fixed emitting wire situated at the centerline of the channel and different grounded electrode arrangements which are in two types of wire and plate. The results indicate that the multi-collector arrangements cause significant changes in the flow pattern, heat transfer coefficient and friction factor. Furthermore, it is found that the thermal enhancement factor has remarkable growth, and EHD efficiency for two types of wire and plate collectors is notable. Besides, as an outstanding result, the electrohydrodynamic phenomenon has a negative effect on the heat transfer enhancement depending upon the values of Reynolds number and applied electric potential.
The nicotine metabolite ratio (NMR; 3‐hydroxycotinine/cotinine) is an index of CYP2A6 activity. CYP2A6 is responsible for nicotine's metabolic inactivation and variation in the NMR/CYP2A6 is ...associated with several smoking behaviors. Our aim was to integrate established alleles and novel genome‐wide association studies (GWAS) signals to create a weighted genetic risk score (wGRS) for the CYP2A6 gene for European‐ancestry populations. The wGRS was compared with a previous CYP2A6 gene scoring approach designed for an alternative phenotype (C2/N2; cotinine‐d2/(nicotine‐d2 + cotinine‐d2)). CYP2A6 genotypes and the NMR were assessed in European‐ancestry participants. The wGRS training set included N = 933 smokers recruited to the Pharmacogenetics of Nicotine Addiction and Treatment clinical trial NCT01314001. The replication cohort included N = 196 smokers recruited to the Quit 2 Live clinical trial NCT01836276. Comparisons between the two CYP2A6 phenotypes and with fractional clearance were made in a laboratory‐based pharmacokinetic study (N = 92 participants). In both the training and replication sets, the wGRS, which included seven CYP2A6 variants, explained 33.8% (P < 0.001) of the variance in NMR, providing improved predictive power to the NMR phenotype when compared with other CYP2A6 gene scoring approaches. NMR and C2/N2 were strongly correlated to nicotine clearance (ρ = 0.70 and ρ = 0.79, respectively; P < 0.001), and to one another (ρ = 0.82; P < 0.001); however reduced function genotypes occurred in slow NMR but throughout C2/N2. The wGRS was able to predict smoking quantity and nicotine intake, to discriminate between NMR slow and normal metabolizers (AUC = 0.79; P < 0.001), and to replicate previous NMR‐stratified cessation outcomes showing unique treatment outcomes between metabolizer groups.
We have developed a weighted genetic risk score (wGRS) for the prediction of the nicotine metabolite ratio (NMR), an index of CYP2A6 activity associated with nicotine's metabolic inactivation and several smoking behaviors. The wGRS model captured 33.8% of the variance in the NMR in both the training set and an external replication cohort, associated with measures of smoking quantity and nicotine intake, and showed the ability to replicate NMR‐based clinical outcomes for slow and normal metabolizers.
South-west Asia including the Middle East is one of the most prone regions to dust storm events. In recent years, there was an increase in the occurrence of these environmental and meteorological ...phenomena. Remote sensing could serve as an applicable method to detect and also characterise these events. In this study, two dust enhancement algorithms were used to investigate the behaviour of dust events using satellite data, compare with numerical model output and other satellite products and finally validate with
in-situ
measurements. The results show that the use of thermal infrared algorithm enhances dust more accurately. The aerosol optical depth from MODIS and output of a Dust Regional Atmospheric Model (DREAM8b) are applied for comparing the results. Ground-based observations of synoptic stations and sun photometers are used for validating the satellite products. To find the transport direction and the locations of the dust sources and the synoptic situations during these events, model outputs (HYSPLIT and NCEP/NCAR) are presented. Comparing the results with synoptic maps and the model outputs showed that using enhancement algorithms is a more reliable way than any other MODIS products or model outputs to enhance the dust.
Preeclampsia (PE) is a severe complication in pregnancy, and its symptoms (proteinuria and hypertension) manifest after 20 weeks of gestation, affecting up to 8 % of pregnancies. The pregnant women's ...immune system uses different tolerance mechanisms to deal with a semi-allogeneic fetus. The T-cell subsets including CD8+, CD4+, and Treg play a critical role in maintaining pregnancies. The expression of immune checkpoint molecules in T-cells can ensure pregnancy at the feto-maternal interface by controlling immune responses. This research aims to evaluate the expression level of immune checkpoint factors, including PD-1, LAG-3, CTLA-4, and TIM-3 in normal pregnant women and PE patients. Decidual tissue was collected from 50 participants (25 PE and 25 control). For evaluating the genes expression, real-time PCR was employed. The western blot was used to assess the proteins level. The results of real-time PCR indicated significantly decreased expression level of these immune checkpoints in PE patients. In parallel to gene expression results, the protein level of PD-1, LAG-3, CTLA-4, and TIM-3 in the PE group was also reduced. We revealed that the profile of proteins and genes expression of immune checkpoints in the decidua of PE mothers are different from normal pregnancy and these results indicate aberrant expression of immune checkpoints such as PD-1, LAG-3, CTLA-4, and TIM-3 may cause maladaptation immune response which results in PE manifestation.
Proteomics is the study of functions and regulation of biological systems based on the analysis of the protein expression profile, and there is a general agreement that soil proteomics may be a tool ...for better soil management. Because of the ability of soils to stabilize extracellular proteins by various mechanisms, development of soil proteomics needs an assessment of the efficiency of protein extraction from various soil types. We evaluated the possibility of extraction of soil microbial proteome by inoculating Cupriavidus metallidurans CH34, which has a known proteome, into sterile sand, kaolinite, montmorillonite and a mixture of sand, kaolinite, montmorillonite, goethite and humic acids. One hour after inoculation, the viability of C. metallidurans was determined by the colony-forming units method (CFU), the amount of extracted proteins was determined by the Bradford method and the bacterial proteome was analysed by the two-dimensional gel electrophoresis technique (2D-GE). The bacterial number was 2.5 × 10⁶ CFU g⁻¹ of soil in all microcosms, whereas the total extracted protein content varied from 98.1 to 1268 µg g⁻¹ in the various microcosms, but was undetectable in the inoculated montmorillonite. The number of protein spots from the bacterial culture and the inoculated microcosms varied between 317 and 591, with 54 variable spots among the pure culture and the microcosms. No protein spots were detected in the 2D-GE from the montmorillonite microcosm. The 2D-GE of artificial soil microcosms showed a protein pattern that was different from those of pure culture and sand and kaolinite microcosms. The results confirm the importance of clay-specific surface area and CEC in protein adsorption as montmorillonite alone had the largest sorptive capacity, and show that the artificial soil used also had a large sorptive capacity for microbial proteins. Globally, the results indicate that the extraction of proteins from soils is strongly influenced by the clay type and organic matter content, and that poor protein extraction efficiency may reduce the potential of soil proteomics.
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