The origin of powered avian flight was a locomotor innovation that expanded the ecological potential of maniraptoran dinosaurs, leading to remarkable variation in modern birds (Neornithes). The avian ...sternum is the anchor for the major flight muscles and, despite varying widely in morphology, has not been extensively studied from evolutionary or functional perspectives. We quantify sternal variation across a broad phylogenetic scope of birds using 3D geometric morphometrics methods. Using this comprehensive dataset, we apply phylogenetically informed regression approaches to test hypotheses of sternum size allometry and the correlation of sternal shape with both size and locomotory capabilities, including flightlessness and the highly varying flight and swimming styles of Neornithes.
We find evidence for isometry of sternal size relative to body mass and document significant allometry of sternal shape alongside important correlations with locomotory capability, reflecting the effects of both body shape and musculoskeletal variation. Among these, we show that a large sternum with a deep or cranially projected sternal keel is necessary for powered flight in modern birds, that deeper sternal keels are correlated with slower but stronger flight, robust caudal sternal borders are associated with faster flapping styles, and that narrower sterna are associated with running abilities. Correlations between shape and locomotion are significant but show weak explanatory power, indicating that although sternal shape is broadly associated with locomotory ecology, other unexplored factors are also important.
These results display the ecological importance of the avian sternum for flight and locomotion by providing a novel understanding of sternum form and function in Neornithes. Our study lays the groundwork for estimating the locomotory abilities of paravian dinosaurs, the ancestors to Neornithes, by highlighting the importance of this critical element for avian flight, and will be useful for future work on the origin of flight along the dinosaur-bird lineage.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Aims. We wish to investigate the physical properties of a sample of Lyα emitting galaxies in the VANDELS survey, with particular focus on the role of kinematics and neutral hydrogen column density in ...the escape and spatial distribution of Lyα photons. Methods. From all the Lyα emitting galaxies in the VANDELS Data Release 2 at 3.5 ≲ z ≲ 4.5, we selected a sample of 52 galaxies that also have a precise systemic redshift determination from at least one nebular emission line (HeII or CIII). For these galaxies, we derived different physical properties (stellar mass, age, dust extinction, and star formation rate) from spectral energy distribution (SED) fitting of the exquisite multiwavelength photometry available in the VANDELS fields, using the dedicated spectral modeling tool BEAGLE and the UV β slope from the observed photometry. We characterized the Lyα emission in terms of kinematics, equivalent width (EW), full width at half-maximum, and spatial extension and then estimated the velocity of the neutral outflowing gas. The ultra-deep VANDELS spectra (up to 80 h on-source integration) enable this for individual galaxies without the need to rely on stacks. We then investigated the correlations between the Lyα properties and the other measured properties to study how they affect the shape and intensity of Lyα emission. Results. We reproduce some of the well-known correlations between Lyα EW and stellar mass, dust extinction, and UV β slope, in the sense that the emission line appears brighter in galaxies with lower mass that are less dusty and bluer. We do not find any correlation with the SED-derived star formation rate, while we find that galaxies with brighter Lyα tend to be more compact in both UV and in Lyα. Our data reveal an interesting correlation between the Lyα velocity offset and the shift of the interstellar absorption lines with respect to the systemic redshift, observed for the first time at high redshifts: galaxies with higher interstellar medium (ISM) outflow velocities show smaller Lyα velocity shifts. We interpret this relation in the context of the shell-model scenario, where the velocity of the ISM and the HI column density contribute together in determining the Lyα kinematics. In support to our interpretation, we observe that galaxies with high HI column densities have much more extended Lyα spatial profiles; this is a sign of increased scattering. However, we do not find any evidence that the HI column density is related to any other physical properties of the galaxies, although this might be due in part to the limited range of parameters that our sample spans.
Curing HIV infection will require the elimination of a reservoir of infected CD4+ T cells that persists despite HIV-specific cytotoxic T cell (CTL) responses. Although viral latency is a critical ...factor in this persistence, recent evidence also suggests a role for intrinsic resistance of reservoir-harboring cells to CTL killing. This resistance may have contributed to negative outcomes of clinical trials, where pharmacologic latency reversal has thus far failed to drive reductions in HIV reservoirs. Through transcriptional profiling, we herein identified overexpression of the prosurvival factor B cell lymphoma 2 (BCL-2) as a distinguishing feature of CD4+ T cells that survived CTL killing. We show that the inducible HIV reservoir was disproportionately present in BCL-2hi subsets in ex vivo CD4+ T cells. Treatment with the BCL-2 antagonist ABT-199 was not sufficient to drive reductions in ex vivo viral reservoirs when tested either alone or with a latency-reversing agent (LRA). However, the triple combination of strong LRAs, HIV-specific T cells, and a BCL-2 antagonist uniquely enabled the depletion of ex vivo viral reservoirs. Our results provide rationale for novel therapeutic approaches targeting HIV cure and, more generally, suggest consideration of BCL-2 antagonism as a means of enhancing CTL immunotherapy in other settings, such as cancer.
The Dinosaur Park Formation (Campanian) of Alberta documents one of the most diverse assemblages of hadrosaurine dinosaurs. Historically, two species of the genus Gryposaurus Lambe, 1914 have been ...recognized in the Dinosaur Park Formation, Gryposaurus notabilis Lambe, 1914 and Gryposaurus incurvimanus Parks, 1919, which are differentiated primarily on their nasal arch morphology. These two species have recently been suggested to represent either variable morphs within G. notabilis (e.g., ontogeny) or two distinct taxa within an evolving Gryposaurus lineage (e.g., anagenesis). These alternative hypotheses have never been adequately tested via detailed morphological comparisons, morphometrics, or biostratigraphy. A geometric morphometric analysis of hadrosaurine skulls from the Dinosaur Park Formation was performed to assess the influence of ontogeny on skull morphology. Gryposaurus incurvimanus skulls were found to be distinctly smaller, and morphologically divergent from those of G. notabilis, with larger G. notabilis skulls having higher nasal arches set farther back on the skull, a size-correlated pattern consistent with ontogenetic nasal retraction documented in other hadrosaurids. Stratigraphic data were used to map this morphology through time, to evaluate the anagenesis hypothesis. The stratigraphic distributions of the two species showed considerable overlap, rejecting anagensis and indicating that the sampled individuals lived over a short period of time (<0.5 Myr). Overall, our results suggest that the hypothesis that G. incurvimanus and G. notabilis represent different ontogenetic stages within a single species cannot be rejected. This study improves our understanding of the extent of potential individual variation within a single Gryposaurus species, which will be useful in assessing the validity of other hadrosaurines.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Immunoediting is a process that occurs in cancer, whereby the immune system acts to initially repress, and subsequently promote the outgrowth of tumor cells through the stages of elimination, ...equilibrium, and escape. Here we present a model for a virus that causes cancer where immunoediting is coordinated through synergistic viral- and host-mediated events. We argue that the initial viral replication process of the Human T cell leukemia virus type I (HTLV-1), which causes adult T cell leukemia/lymphoma (ATL) in ~5% of individuals after decades of latency, harmonizes with the host immune system to create a population of cells destined for malignancy. Furthermore, we explore the possibility for HIV to fit into this model of immunoediting, and propose a non-malignant escape phase for HIV-infected cells that persist beyond equilibrium.
Epidemic forecasting and prediction tools have the potential to provide actionable information in the midst of emerging epidemics. While numerous predictive studies were published during the ...2016-2017 Zika Virus (ZIKV) pandemic, it remains unknown how timely, reproducible, and actionable the information produced by these studies was.
To improve the functional use of mathematical modeling in support of future infectious disease outbreaks, we conducted a systematic review of all ZIKV prediction studies published during the recent ZIKV pandemic using the PRISMA guidelines. Using MEDLINE, EMBASE, and grey literature review, we identified studies that forecasted, predicted, or simulated ecological or epidemiological phenomena related to the Zika pandemic that were published as of March 01, 2017. Eligible studies underwent evaluation of objectives, data sources, methods, timeliness, reproducibility, accessibility, and clarity by independent reviewers.
2034 studies were identified, of which n = 73 met the eligibility criteria. Spatial spread, R0 (basic reproductive number), and epidemic dynamics were most commonly predicted, with few studies predicting Guillain-Barré Syndrome burden (4%), sexual transmission risk (4%), and intervention impact (4%). Most studies specifically examined populations in the Americas (52%), with few African-specific studies (4%). Case count (67%), vector (41%), and demographic data (37%) were the most common data sources. Real-time internet data and pathogen genomic information were used in 7% and 0% of studies, respectively, and social science and behavioral data were typically absent in modeling efforts. Deterministic models were favored over stochastic approaches. Forty percent of studies made model data entirely available, 29% provided all relevant model code, 43% presented uncertainty in all predictions, and 54% provided sufficient methodological detail to allow complete reproducibility. Fifty-one percent of predictions were published after the epidemic peak in the Americas. While the use of preprints improved the accessibility of ZIKV predictions by a median of 119 days sooner than journal publication dates, they were used in only 30% of studies.
Many ZIKV predictions were published during the 2016-2017 pandemic. The accessibility, reproducibility, timeliness, and incorporation of uncertainty in these published predictions varied and indicates there is substantial room for improvement. To enhance the utility of analytical tools for outbreak response it is essential to improve the sharing of model data, code, and preprints for future outbreaks, epidemics, and pandemics.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Utilizing spectroscopic observations taken for the VIMOS Ultra-Deep Survey (VUDS), new observations from Keck/DEIMOS, and publicly available observations of large samples of star-forming galaxies, we ...report here on the relationship between the star-formation rate (SFR) and the local environment (
δ
gal
) of galaxies in the early universe (2 <
z
< 5). Unlike what is observed at lower redshifts (
z
≲ 2), we observe a definite, nearly monotonic increase in the average SFR with increasing galaxy overdensity over more than an order of magnitude in
δ
gal
. The robustness of this trend is quantified by accounting for both uncertainties in our measurements and galaxy populations that are either underrepresented or not present in our sample (e.g., extremely dusty star-forming and quiescent galaxies), and we find that the trend remains significant under all circumstances. This trend appears to be primarily driven by the fractional increase of galaxies in high-density environments that are more massive in their stellar content and are forming stars at a higher rate than their less massive counterparts. We find that, even after stellar mass effects are accounted for, there remains a weak but significant SFR–
δ
gal
trend in our sample implying that additional environmentally related processes are helping to drive this trend. We also find clear evidence that the average SFR of galaxies in the densest environments increases with increasing redshift. These results lend themselves to a picture in which massive gas-rich galaxies coalesce into proto-cluster environments at
z
≳ 3, interact with other galaxies or with a forming large-scale medium, subsequently using or losing most of their gas in the process, and begin to seed the nascent red sequence that is present in clusters at slightly lower redshifts.
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•Despite spectral similarities, red meat species can be discriminated using Raman spectroscopy.•PCA, PLS-DA and SVM were successful in discriminating intact red meat species.•Raman ...spectroscopy looks promising for meat sorting and authentication.
With increasing demand for fast and reliable techniques for intact meat discrimination, we explore the potential of Raman spectroscopy in combination with three chemometric techniques to discriminate beef, lamb and venison meat samples. Ninety (90) intact red meat samples were measured using Raman spectroscopy, with the acquired spectral data preprocessed using a combination of rubber-band baseline correction, Savitzky-Golay smoothing and standard normal variate transformation. PLSDA and SVM classification were utilized in building classification models for the meat discrimination, whereas PCA was used for exploratory studies. Results obtained using linear and non-linear kernel SVM models yielded sensitivities of over 87 and 90 % respectively, with the corresponding specificities above 88 % on validation against a test set. The PLSDA model yielded over 80 % accuracy in classifying each of the meat specie. PLSDA and SVM classification models in combination with Raman spectroscopy posit an effective technique for red meat discrimination.
Alzheimer's disease (AD) involves a gradual breakdown of brain connectivity, and network analyses offer a promising new approach to track and understand disease progression. Even so, our ability to ...detect degenerative changes in brain networks depends on the methods used. Here we compared several tractography and feature extraction methods to see which ones gave best diagnostic classification for 202 people with AD, mild cognitive impairment or normal cognition, scanned with 41-gradient diffusion-weighted magnetic resonance imaging as part of the Alzheimer's Disease Neuroimaging Initiative (ADNI) project. We computed brain networks based on whole brain tractography with nine different methods - four of them tensor-based deterministic (FACT, RK2, SL, and TL), two orientation distribution function (ODF)-based deterministic (FACT, RK2), two ODF-based probabilistic approaches (Hough and PICo), and one "ball-and-stick" approach (Probtrackx). Brain networks derived from different tractography algorithms did not differ in terms of classification performance on ADNI, but performing principal components analysis on networks helped classification in some cases. Small differences may still be detectable in a truly vast cohort, but these experiments help assess the relative advantages of different tractography algorithms, and different post-processing choices, when used for classification.
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