The strict tolerance space of three-dimensional (3D) crystalline structures is still a significant challenge in the area of switching dielectrics in comparison with lower-dimensional structures. ...Generally, the function of crystalline materials can be given or adjusted by controlling the environment in which synthesis takes place or the packing rearrangement. Using this method, special functional enhancements or changes in the dielectrics can be realized by improving the synthetic strategies. Here, a 3D switchable dielectric compound MeHdabcoK(BF
4
)
3
was achieved by employing the temperature selective effect. In particular, its structure is completely different from the usual 3D perovskite structure, which is constructed using two different cation-template frameworks. Moreover, the 3D MeHdabcoK(BF
4
)
3
shows a structural phase transition at 358 K. The thermal analysis (differential scanning calorimetry (DSC)) and X-ray diffractometry results provided evidence of these phase changes. This work provides a feasible strategy that can be used to achieve the different structures of an 'isomer', and enrich the method used for designing diverse functional materials.
A unique three-dimensional hybrid material MeHdabcoK(BF
4
)
3
was obtained through a temperature-selective method, and demonstrates a switchable dielectric response.
We construct a simple fluorescent biosensor for single-molecule counting of flap endonuclease 1 (FEN1) based on ligase detection reaction (LDR) amplification-activated CRISPR-Cas12a. This biosensor ...exhibits excellent selectivity and high sensitivity with a detection limit (LOD) of 1.31 × 10
−8
U. Moreover, it can be employed to screen the FEN1 inhibitors and quantitatively measure the FEN1 activity in human cells and breast cancer tissues, holding great promise in clinical diagnosis and drug discovery.
We construct a simple fluorescent biosensor for single-molecule counting of FEN1 based on ligase detection reaction (LDR) amplification-activated CRISPR-Cas12a.
The present study was conducted to analyze the level of four priority polycyclic aromatic hydrocarbons (PAHs), including benzoapyrene (BaP), chrysene (Chr), benzoaanthracene (BaA), and ...benzobfluoranthene (BbF), in traditionally smoked chicken products marketed in China. The results show that the amount of ƩPAH4 (the sum of four different PAHs: BaP, Chr, BaA, and BbF) was 30.43-225.17 and 18.75-129.54 µg/kg in the skin and meat of smoked chicken products, respectively. The content of ƩPAH4 in the smoked skin was significantly higher as compared to the smoked meat (
< 0.05). The calculation of MOE (margin of exposure) results suggested the possibilities of ingestion risk associated with the consumption of smoked chicken skin. Furthermore, the formaldehyde content in the skin of smoked chicken was 2.17-6.84 mg/kg and 0.86-2.95 mg/kg in the smoked meat. These results indicate that optimization or alternative methods for food processing should be developed to reduce the high level of harmful substances formed during processing to ensure the safety of smoked chicken products. Moreover, along with harmful substances, the moisture content and color of traditionally smoked chicken were analyzed to provide a practical reference for healthy, safe and green processing technology for smoked chicken.
CCN1 is well studied in terms of its functions in injury repair, cell adhesion survival and apoptosis, bacterial clearance and mediation of inflammation-related pathways, such as the TLR2/4 pathways. ...However, the role of CCN1 protein and its interaction with TLR2/4 pathways in intestinal epithelial cells was not elucidated after
infection. The results of this study confirm that
infection induced intestinal inflammation and increased the protein expression of CCN1, TLR2, TLR4 and p38, which followed a similar tendency in the expression of genes related to the TLR2/4 pathways. In addition, organoids infected by
showed a significant increase in the expression of CCN1 and the activation of TLR2/4 pathways. Furthermore, pre-treatment with CCN1 protein to organoids infected by
could increase the related genes of TLR2/4 pathways and up-regulate the expression of
, and increase the count of pathogens in organoids, which indicates that the interaction between the CCN1 protein and TLR2/4 signaling pathways in intestinal epithelial cells occurred after
infection. This study will provide a novel insight of the role of CCN1 protein after
infection in the intestine.
Electric water pumps (EWPs) are characterized by small sizes and variable operating conditions. Efficient operation of such a pump is important for the whole thermal management system. Here, ...comprehensive hydraulic optimization was performed to improve the performance of an EWP. The computational fluid dynamics technique was used to assist in the optimization. The numerical scheme was validated using experimental data. Variations in the blade number, blade profile, and blade inlet edge were implemented. The results showed that solely increasing the blade number leads to an increase in the pump head but cannot guarantee high pump efficiency. A combination of adjusting the blade wrap angle and extending the blade inlet edge toward the pump inlet promotes increases in both the pump head and efficiency. With the improvement in pump performance, the characteristics of the flow field alter remarkably. The pressure at the blade inlet is reduced, and the turbulent kinetic energy near the impeller blade decreases overall.
Anabolic androgenic steroids (AASs) are usually illegally added to animal feed because they can significantly promote animal growth and increase carcasses' leanness, which threatens the safety of ...animal-derived foods and indirectly hazards human health. This study aimed to establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous detection of twelve AAS residues in livestock and poultry meat. The homogenized samples were extracted with acetonitrile containing 1% acetic acid (
/
) and purified using the one-step extraction column. After concentration using nitrogen, the residues were redissolved in acetonitrile and then quantified with an external standard method using UHPLC-MS/MS. The results showed that the above-mentioned method had a satisfactory linear correlation (
≥ 0.9903) with a concentration range of 1-100 μg/L, and the limits of detection (LODs) and quantification (LOQs) were 0.03-0.33 μg/kg and 0.09-0.90 μg/kg, respectively. With the intraday and interday precision less than 15%, the average recoveries of pork, beef, lamb, and chicken, at different spiked levels, ranged from 68.3 to 93.3%, 68.0 to 99.4%, 71.6 to 109.8%, and 70.5 to 97.7%, respectively. Overall, the established method is validated, precise, and capable of the high-throughput determination of the residues of twelve AASs in livestock and poultry meat.
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•The XHP inhibited the secretion of NO, IL-6 and TNF-α in LPS-induced RAW264.7 cell.•Peak 4 fraction possessed stronger anti-inflammatory activity than other factions after ...purification by G10.•Peak 4 fraction reduced the mRNA expression of Ppp21b and Spp1 in PI3K/AKT signal pathway.
Anti-inflammatory activities of Xuanwei ham crude peptides (XHP) were evaluated according to the inhibition of lipopolysaccharide (LPS) induced RAW264.7 cell secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α (TNF-α). After the separation by Sephadex G-10, the Peak 4 fractions showed stronger inhibition effects on the secretion of NO, IL-1β and IL-8 and exhibited the strongest suppression on IL-6 mRNA expression. Proteomic analysis showed that Peak 4 fraction achieved the effect of regulating inflammation by reducing the expression of Ppp2r1b and Spp1 which were involved in inflammation-related phosphatidylinositol-3 kinase/serine threonine kinase(PI3K/AKT) signaling pathways. There were 36 peptide sequences identified in Peak 4 fraction in which GPAGPL and GPPGAP exhibited much higher anti-inflammatory capacity by decreasing the secretion of NO and IL-6. Based on the above results, the Xuanwei ham derived peptides were firstly reported to possess the anti-inflammatory effect in LPS induced RAW264.7 cells.
The present study aims to reveal wear characteristics of an impeller pump transporting the mixture of liquid and solid particles. An experimental work is conducted to detect patterns of wear of the ...pump components. The computational fluid dynamics (CFD) technique and the discrete element method (DEM) are jointly used to simulate the two-phase flow and wear of the pump components. After a 30-hour wear experiment, the inlet edge of the long blades exhibits wavy profiles, and the outlet part of the long blades is worn through. Regarding the volute, only the volute tongue is severely worn. At a particle diameter of 2.0 mm and a solid mass fraction of 10 %, the ratio of the average wear depth of the long blades to that of the volute reaches 64.5. Wear of the blade inlet edge depends on the normal cumulative contact energy, while the tangential cumulative contact energy contributes significantly to wear of the outlet part of the long blades. As the particle diameter increases from 1.0 mm to 3.0 mm, the average depth of wear of the long blades decreases monotonically by nearly 45 %, but the maximum depth of wear increases by about 34 %. The solid mass fraction serves as a key factor influencing wear of the pump components, especially when particles of large size are transported.
•The Pb-based perovskite exhibits a red-light photoluminescence.•The dual phase changes of photoluminescence and dielectric were shown.•The step-like dielectric switching was observed at higher ...temperature.
Intelligent multifunctional materials are indispensable for the electronic devices, but the existing applied counterparts have the disadvantage that their structures cannot be adjusted to meet diversified application requirements. Therefore, organic-inorganic hybrid perovskites with the inherent advantage of structural diversity are expected to become promising intelligent multifunctional materials. Based on the diversity of perovskites mentioned above, we have reported a series of dielectric switches. Here, a new one-dimensional organic-inorganic hybrid perovskite (N-fluoroethyl-N-methylmorpholine)PbBr3 was reported. In this distorted octahedral perovskite, a step-like dielectric switching was observed at around 343 K upon heating and 325 K upon cooling. Simultaneously, photoluminescence is also related to the highly distorted octahedron, and this one-dimensional Pb-based halide exhibits a red-light with CIE chromaticity coordinates of (0.56, 0.42) upon ultraviolet photoexcitation. In short, the reversible/switchable dielectric phase transition and photoluminescence characteristic in this hybrid perovskite was successfully achieved, which are expected to be applied in optoelectronic devices and dielectric switches, and promote the development of organic-inorganic hybrid multifunctional materials.
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The present study aims to describe the characteristics of the hydraulic transport of manganese nodules in a vertical pipe. The solid–liquid two-phase flows were simulated using a numerical technique ...that combines the computational fluid dynamics (CFD) method and the discrete element method (DEM). Manganese nodules with diameters of 5.0 mm, 15.0 mm, and 30.0 mm were selected. The effects of the initial solid volume fraction and the initial mixture velocity were investigated. The results show that with increasing initial solid volume fraction, the liquid and solid velocities decrease but the total pressure drop over the pipe increases. Small particles are responsible for high particle collision frequency, which causes decreases in both the liquid velocity and the total pressure drop. Energy loss is aggravated by increasing the initial mixture velocity, manifesting in the increase of the total pressure drop. The retention ratio of manganese nodules varies inversely with the initial mixture velocity. A formula is proposed to describe the pressure drop due to the presence of solid particles and collisions.
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