Samples from naked oat were steeped and germinated under controlled conditions in an incubator. Changes of phenolic compounds and antioxidant activity were investigated in oats during steeping and ...germination. Results revealed that phenolic compounds and antioxidant activity of oats varied with the difference in steeping and germination stages. Compared with raw grains, short-term steeping treatment did not show significant effects (p > 0.05) on phenolic content. Germination can significantly result in the decrease in bound phenolic and the increase in free and total phenolics. Main phenolic acids and avenanthramides were isolated and quantified by HPLC analysis. During steeping, phenolic acids decreased (p < 0.05); avenanthramide N-(3′,4′-dihydroxy)-(E)-cinnamoyl-5-hydroxyanthranilic acid first decreased and then increased (p < 0.05), while avenanthramides N-(4′-hydroxy)-(E)-cinnamoyl-5-hydroxyanthranilic acid and N-(4′-hydroxy-3′-methoxy)-(E)-cinnamoyl-5-hydroxyanthranilic acid did not change significantly (p > 0.05). During germination, gallic and caffeic acids first increased (p < 0.05) and then decreased, whereas p-coumaric and ferulic acids and avenanthramides increased (p < 0.05). Nevertheless, avenanthramides did not change significantly (p > 0.05) during the last stage of germination. Oat extracts exhibited increasing high antioxidant activity with the steeping and germination going on, which may explain that antioxidant activity correlated (p < 0.01) significantly with the content of phenolic compounds.
Exposure to social stress and dysregulated serotonergic neurotransmission have both been implicated in the etiology of psychiatric disorders. However, the serotonergic circuit involved in stress ...vulnerability is still unknown. Here, we explored whether a serotonergic input from the dorsal raphe (DR) to ventral tegmental area (VTA) influences vulnerability to social stress. We identified a distinct, anatomically and functionally defined serotonergic subpopulation in the DR that projects to the VTA (5-HT
neurons). Moreover, we found that susceptibility to social stress decreased the firing activity of 5-HT
neurons. Importantly, the bidirectional manipulation of 5-HT
neurons could modulate susceptibility to social stress. Our findings reveal that the activity of 5-HT
neurons may be an essential factor in determining individual levels of susceptibility to social stress and suggest that targeting specific serotonergic circuits may aid the development of therapies for the treatment of stress-related disorders.
Anisotropic nanoparticles have been widely used as building blocks for preparing surface-enhanced Raman spectroscopy (SERS) substrates. However, tailoring the SERS activity at the self-assembly level ...through the anisotropic nanoparticle orientation is a big challenge, mainly due to the lack of simple assembly methods. In the present work, we report an air-water interface mediated co-assembly (AWIMCoA) strategy to prepare flexible 2D superlattices of Au octahedra with tunable orientations. We have demonstrated that Au octahedra can self-assemble into face-up, edge-up and vertex-up orientations on changing the surface wettability of Au octahedra, which determines the interparticle anisotropic interactions and the interaction between Au octahedra and the poly(styrene-ethylene-butylene-styrene) (SEBS) nanomembrane. The effect of assembly orientation on the SERS performance of 2D superlattices has been studied through correlated SEM characterization and SERS mapping. Among all the orientational modes, flexible 2D superlattices with the vertex-up orientation show the highest enhancement performance and uniformity, which is further demonstrated by theoretical simulation. Partially embedded 2D superlattices in the SEBS nanomembrane are robust to remove the surface ligands without breaking the whole nanostructure. This post-treatment process boosts the SERS performance of the 2D superlattice with the edge-up orientation by forming fused nanostructures among neighboring Au octahedra. We expect that the co-assembly method will be widely applied in the preparation of reusable and high-performance SERS substrates for broad application.
The use of imaging technologies has been critical in deciphering biological phenomena, structures, and mechanisms across a wide range of spatial scales. The spatial resolution of traditional imaging ...modalities cannot meet the needs of high-precision research and diagnosis in biomedical fields. Plasmon resonance is the light-matter interaction that allows localizing far-field radiation in the near field with an intense electromagnetic field, enhancing the nanometric ablation, elastic/inelastic scattering of the adsorbate, and photoluminescence of the fluorophore nearby. Further, plasmon resonance scattering of nanoparticles can sensitively indicate the local environmental changes. This is accomplished by combining the spatially resolved capability with molecular spectrometry techniques such as Raman, infrared, fluorescence,
etc.
, leading to a series of excellent imaging techniques to interrogate diverse biological processes from the tissue to subcellular level. In this tutorial review, we first provide the fundamental aspects of plasmonics. Then we give a systematic discussion of the working principle of these plasmon-based imaging techniques with an emphasis on the achievable spatial resolutions: surface-enhanced Raman spectroscopy (micrometre to nanometre), tip-enhanced ablation and ionization mass spectrometry (submicrometre), scattering-type scanning near-field optical microscopy (nanometre), tip-enhanced Raman spectroscopy (nanometre), tip-enhanced fluorescence spectroscopy (nanometre), and plasmon/molecular ruler microscopy (nanometre to angstrom). We also review the recent developments of the bioimaging applications of these techniques and expect that the plasmon-based techniques will not only pave a new way to decipher mysteries in life sciences but also hold great potential to be extended from fundamental research studies to real-life biomedical applications.
Plasmonic technologies are available for multi-scale biomedical imaging ranging from micrometre to angstrom level.
Abstract
Neutron stars (NSs) play essential roles in modern astrophysics. The magnetic fields and spin periods of newborn (zero-age) NSs have a large impact on the further evolution of NSs, which ...are, however, poorly explored in observations due to the difficulty of finding newborn NSs. In this work, we aim to infer the magnetic fields and spin periods (
B
i
and
P
i
) of zero-age NSs from the observed properties of the NS population. We select nonaccreting NSs whose evolution is solely determined by magnetic dipole radiation. We find that both
B
i
and
P
i
can be described by lognormal distribution, and the fitting sensitively depends on our parameters.
Spermiogenesis is a highly orchestrated developmental process during which chromatin condensation decouples transcription from translation. Spermiogenic mRNAs are transcribed earlier and stored in a ...translationally inert state until needed for translation; however, it remains largely unclear how such repressed mRNAs become activated during spermiogenesis. We previously reported that the MIWI/piRNA machinery is responsible for mRNA elimination during late spermiogenesis in preparation for spermatozoa production. Here we unexpectedly discover that the same machinery is also responsible for activating translation of a subset of spermiogenic mRNAs to coordinate with morphological transformation into spermatozoa. Such action requires specific base-pairing interactions of piRNAs with target mRNAs in their 3′ UTRs, which activates translation through coupling with cis-acting AU-rich elements to nucleate the formation of a MIWI/piRNA/eIF3f/HuR super-complex in a developmental stage-specific manner. These findings reveal a critical role of the piRNA system in translation activation, which we show is functionally required for spermatid development.
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•MIWI/piRNA activates mRNA translation via imperfect base-pairing interactions•HuR and eIF3f are required for MIWI/piRNA-mediated target mRNA activation•piRNA system controls the translation of a subset of mRNAs in mouse spermatids•piRNA system plays a central role in acrosome formation during spermiogenesis
The piRNA pathway, through functional interplay with HuR and eIF3f, plays an important role in translational activation of a specific set of mRNAs during mouse spermiogenesis.
The realization of surface-enhanced Raman spectroscopy (SERS) to be a reliable quantitative analytical technique requires sensitive and reproducible enhancing substrates. Here, uniform ...three-dimensional (3D) Ag nanorod (AgNR) substrates with well-defined interlayer spacings are prepared through the air–liquid interface-assisted self-assembly of AgNR in a layer-by-layer manner. The correlation of the SERS performance with the 3D AgNR structures is performed by SERS mapping the substrates. SERS mapping reveals the excellent enhancement uniformity of the 3D substrates with the relative standard deviation (RSD) less than 10%. It finds that both of the number of layers (NL) and the length of the AgNR have effects on the SERS performance of the 3D AgNR substrates. It is demonstrated that the intergaps between layers contribute much to the SERS intensity of the 3D AgNR by creating the interlayer (out-of-plane) plasmonic coupling. The impact of the excitation wavelengths (532, 633, and 785 nm) on SERS performance is also determined. The optimal 3D AgNR structures achieved by the correlation study is further used to detect a set of related molecules (l-tryptophan (Trp), l-phenylalanine (Phe), urea, and melamine). The 3D AgNR SERS of the analytes exhibits linear responses over wide concentration ranges. The sensitivity of the 3D AgNR SERS is proved by comparing to that of the current methods. Moreover, the 3D AgNR substrates maintain the performance stability during 4 weeks of storage.
Three-dimensional (3D) plasmonic nanostructures are emerging as excellent surface-enhanced Raman spectroscopy (SERS) substrates for chemical and biomedical applications. However, the correlation of ...3D (including both in-plane and out-of-plane) plasmonic coupling with the SERS properties to deepen the understanding of 3D SERS substrates remains a challenge. Here, we perform correlation studies of 3D plasmonic coupling and SERS properties of the 3D hierarchical SERS substrates by tuning the multiscale structural elements. The effects of zero-dimensional (0D; the size of the building blocks), one-dimensional (1D; the thickness of the 3D substrates), and two-dimensional (2D; the composition of individual monolayers) structural elements on 3D plasmonic coupling are studied by performing UV–vis–near-infrared (NIR) spectroscopy and measuring SERS performance. It shows that both the extinction spectra and SERS enhancement are tuned at the 3D structural level. It is demonstrated that the plasmonic resonance wavelength (PRW) stemming from the 3D plasmonic coupling correlates with the SERS averaged surface enhancement factor (ASEF) and is improved by more than tenfold at the optimum 3D nanostructure. The optimized substrate is used to quantitatively analyze two small biological molecules. Moreover, as a proof-of-concept study, the substrate is first applied to differentiate between living liver normal and cancer cells with a high prediction accuracy through the spectral features of the cell membranes and the metabolites secreted outside the cells. We expect that the tuning of plasmonic coupling at the 3D level can open up new routes to design high-performance SERS substrates for wide applications.
Summary
Transmission rate and role in hosts contribute to the prevalence of an endosymbiont. However, factors affecting transmission and role of facultative endosymbionts are still not well ...understood. Here, we illustrated that host plants and environmental temperatures affected the transmission, relative abundance and role of Arsenophonus in the cotton aphid Aphis gossypii. The transmission rate of this endosymbiont from mother aphids to offspring was relatively lower. High temperatures impeded the transmission, and infection rates declined as aphids were exposed to 30°C. Contents of amino acids and secondary metabolites were remarkably different among host plants. Aphids feeding on zucchini leaves containing a higher titre of amino acids and lower secondary metabolites harboured a relatively lower abundance of Arsenophonus. Concentrations of an amino acid and a plant secondary metabolite, cucurbitacin B, in aphid diet were not associated with Arsenophonus abundance. However, gossypol, another plant secondary metabolite, was strongly related with the abundance. Arsenophonus imparted a fitness benefit to aphids, and the benefit was dependent on host plants and gossypol concentration. In sum, plant secondary metabolite and environmental temperature affect transmission, relative abundance and role of Arsenophonus, which determine the endosymbiont prevalence in aphid populations.
Several black hole low-mass X-ray binaries (BHLMXBs) show very fast orbital shrinkage, which is difficult to understand in the standard picture of the LMXB evolution. Based on the possible detection ...of a circumbinary (CB) disk in A0620-00 and XTE J1118+480, we investigate the influence of the interaction between a CB disk and the inner binary and calculate the evolution of the binary using the Modules for Experiments in Stellar Astrophysics. We consider two cases for the CB disk formation in which it is fed by mass loss during single outburst or successive outbursts in the LMXB. We show that when taking reasonable values of the initial mass and the dissipating time of the disk, it is possible to explain the fast orbital shrinkage in the BHLMXBs without invoking a high-mass transfer rate.
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