Following the development of co-branding and the emergence of the co-branded product boom, many consumers are choosing to buy their favorite co-branded products. Among them, co-branded food products ...occupy a large share of the market. However, the rapid development of co-branded products has also given rise to many problems, such as the mismatched positioning of the two co-branded enterprises and the poor quality of their products, all of which seriously affect consumers' purchase motivation and satisfaction as well as the development of the co-branded enterprises. Against this background, this paper presents a quantitative analysis of consumer motivation, purchasing psychology and satisfaction, and reviews three aspects of co-branded food purchasing motivation, satisfaction analysis, and ways to improve consumer satisfaction. This paper reflects the research findings in this field, the reality and the problems to be addressed. Taking co-branded food products as an example, this paper analyzes the relevant factors affecting consumer motivation and satisfaction of co-branded food products by studying theories related to co-branding model, consumer motivation and satisfaction, and combining the results of questionnaire research with relevant theories and charts. It also proposes systematic suggestions for raising consumer motivation and satisfaction to help enterprises stand out in the face of fierce competition.
Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model development and livestock breeding. Despite ongoing attempts since ...the 1990s, no stably defined pig epiblast-derived stem cell line has been established. Here, guided by insights from a large-scale single-cell transcriptome analysis of pig embryos from embryonic day (E) 0 to E14, specifically, the tracing of pluripotency changes during epiblast development, we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts (pgEpiSCs). Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK, JAK/STAT3, and Activin/Nodal pathways, pgEpiSCs maintain their pluripotency transcriptome features, similar to those of E10 epiblast cells, and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers. Strikingly, ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripotency marker genes in pgEpiSCs. In practice, we confirmed that pgEpiSCs readily tolerate at least three rounds of successive gene editing and generated cloned gene-edited live piglets. Our findings deliver on the long-anticipated promise of pig pluripotent stem cells and open new avenues for biological research, animal husbandry, and regenerative biomedicine.
Osteoarthritis (OA) is a common joint disease, and studies have reported that the endoplasmic reticulum stress (ERS) in chondrocytes caused by the cartilage tissue damage could mediate the activation ...of Nod-like receptor protein 3 (NLRP3) inflammasomes through inositol-requiring enzyme 1 alpha (IRE1α) and thioredoxin interacting protein (TXNIP). Ginsenoside compound K (CK) has an inhibitory effect on IRE1α activation. However, the role of IRE1α-TXNIP and its interaction with CK are still unclear. In this study, we examined the role and mechanism of action of CK in OA. We found that CK ameliorated OA and ERS in IL-1β-treated chondrocytes and a monoiodoacetate-induced rat OA model. The effect of CK on inflammation, pyroptosis, and ERS was blocked by the ERS inducer tunicamycin. In conclusion, CK hindered OA progression by inhibiting the ERS-IRE1α-TXNIP-NLRP3 axis. Overall, our data indicate that CK could be useful in the treatment of OA and other chronic inflammatory diseases.
This study aimed to explore the effects of working memory updating training on primary school students' writing ability and performance.
A total of 46 fourth-grade Chinese primary school students ...were recruited; their performance in the Chinese character N-back training task, the Writing Ability Questionnaire, and a time-limited writing task was assessed.
The paired-sample
-test revealed that working memory updating training significantly improved the experimental group's working memory level. After training, a repeated measures ANOVA revealed that the experimental group's performance on the Writing Ability Questionnaire improved and was higher than that of the control group. In the time-limited writing task, independent-sample
-tests revealed that the experimental group's writing fluency increased and was higher than that of the control group, while the latter's grammatical accuracy and complexity decreased and were lower than those of the former.
Working memory updating training can be used as auxiliary cognitive training to improve primary school students' working memory level, thereby promoting their writing development.
•Land use and climate change impacts on ecosystem services are distinguished.•The changes of NPP and water yield are mainly dominated by land use change.•The change of soil retention is mainly ...dominated by climate change.•Land use change impact on ecosystem services is highly sensitive to urbanization.
Land use and climate change are the most important factors driving the change of ecosystem services (ESs). It is critical to understand the mechanisms behind such changes for improving ESs. However, existing studies mainly focuse on ES changes due to combined effects of land use and climate change, while the interactions between these factors and ESs remain unclear. This study took Guizhou Province, China as the case study area and examined the impact of land use and climate change on three ESs, i.e. net primary productivity (NPP), soil retention, and water yield. The impacts of land use and climate change on ESs were compared within and between different zones (including terrain zones, geomorphic zones, and urban development zones). The results showed that all the three ESs were characterized with high values in the south and low values in the north, and an overall increasing trend during 2000–2015. Changes in NPP and water yield were dominated by land use change, accounting for 85.2% and 74.9% of the total area, respectively, whereas for soil retention it was climate change, accounting for 71.0% of area proportion. As to zoning contrast, the impact of climate change on NPP and water yield differed the most across geomorphic zones, with terrain zones for soil retention. The impact of land use change on all the three ESs behaved the most difference across urban development zones, indicating high sensitivity of ESs to human activity.
After the new left ventricular ejection fraction (LVEF) classification criteria emerged, many studies have focused on the differences between heart failure (HF) with reduced EF (HFrEF), HF with ...midrange EF (HFmrEF), and HF with preserved EF (HFpEF). However, the lack of consensus on sex-related differences in prognosis within the new standard remains. We aimed to explore sex differences in the clinical characteristics and prognoses of Chinese inpatients with HF defined according to the new standard.From March 2014 to February 2016, 2284 patients with symptomatic HF were consecutively recruited to this prospective research. Case data and 2-year follow-up observations were used to identify sex differences in clinical characteristics and prognoses.When comparing men and women with HFrEF, HFmrEF, and HFpEF, women were older, were more likely to be hospitalized for the first diagnosis of HF, and had lower mean LVEF. Women had a higher tendency of all-cause mortality than did men at 3, 12, and 24 months following HF. After multivariate adjustment, the hazard ratios (HRs) for 24-month all-cause mortality for HFrEF, HFmrEF, and HFpEF were 1.113 (0.728, 1.704), P = 0.620; 1.063 (0.730, 1.548), P = 0.750; and 0.619 (0.240, 1.593), P = 0.320, for men versus women, respectively.There were some sex differences in the clinical characteristics of patients with symptomatic HF in HFrEF, HFmrEF, and HFpEF, but women and men had comparable outcomes over the 2-year period following hospitalization.
Abstract
Zinc ion batteries (ZIBs) have recently attracted tremendous interest for being low‐cost, environmentally benign, and high energy density. However, the large‐scale practical application of ...ZIBs is hampered by well‐known undesirable dendrite growth and serious side reactions of the Zn anode during the long‐term cycling process. Herein, a multifunctional water‐glass artificial protection layer with enormous Si─O functional groups is constructed on Zn anode through a simple spin‐coating method. The theoretical and experimental investigation suggests that the as‐constructed interface with rich Si─O hydrophilic functional groups on Zn anode could facilitate the even distribution of electric field distribution and homogeneous wettability, navigate uniform zinc deposition/stripping along the (002) plane, and subsequently lead to well‐suppressed dendrite growth and effective prohibition of oxygen‐involved corrosion. Consequently, the water glass‐modified anode achieves highly reversible Zn plating/stripping over 1500 h at a high current density of 10 mA cm
−2
in symmetrical cells, and a high capacity retention ratio of 79.4% at the current density of 5 A g
−1
in full cells paired with V
2
O
5
cathode. This proposed water glass coating layer design is cheap, up‐scalable, and facile, which could substantially accelerate the rapid commercialization of zinc anodes and unleash the full potential of renewable ZIBs for next‐generation large‐scale energy storage.
In this work, we designed a simple, disposable, highly sensitive electrochemical aptasensor to detect di(2-ethylhexyl) phthalate (DEHP) in edible vegetable oils. The aptasensor was created by ...decorating polyaniline multi-walled carbon nanotubes (PANI-MWCNTs), Cu metal-organic framework (CuMOF), and gold nanoparticles (AuNPs) on a screen printing carbon electrode (SPCE). With its large surface area, CuMOF modified the electrode by improving the adhesion rate of gold nanoparticles, thereby improving the detection limit of the sensor. The distinct peak in the square wave voltammetry (SWV) was used to measure DEHP. The sensor had high stability, excellent repeatability, reproducibility, and specificity, with a wide linear range (0.1–100 ng/mL) and a low detection limit (0.03 ng/mL). When the aptasensor was used to analyze the DEHP content in edible vegetable oils, the recovery rate was between 95.11 % and 98.29 %, and the results were consistent with the GC-MS measurements.
•An aptasensor modified by nanomaterials on SPCE was designed to detect DEHP.•The linear range of aptasensor is from 0.1 to 100 ng/mL with a LOD of 0.03 ng/mL.•This sensor can be applied to measure the content of DEHP in vegetable oils.
Networks are powerful tools to uncover functional roles of genes in phenotypic variation at a system-wide scale. Here, we constructed a maize network map that contains the genomic, transcriptomic, ...translatomic and proteomic networks across maize development. This map comprises over 2.8 million edges in more than 1,400 functional subnetworks, demonstrating an extensive network divergence of duplicated genes. We applied this map to identify factors regulating flowering time and identified 2,651 genes enriched in eight subnetworks. We validated the functions of 20 genes, including 18 with previously unknown connections to flowering time in maize. Furthermore, we uncovered a flowering pathway involving histone modification. The multi-omics integrative network map illustrates the principles of how molecular networks connect different types of genes and potential pathways to map a genome-wide functional landscape in maize, which should be applicable in a wide range of species.
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