Objective To determine whether cytomorphometric differences of multinucleated giant cells (MGCs) and CD68 reactivity of both MGCs and infiltrating macrophages may be associated with the clinical ...behavior of central and peripheral giant cell lesions of the jaws. Study design Paraffin-embedded samples of central giant cell lesions (CGCLs; n = 20) and peripheral giant cell lesions (PGCLs; n = 20) were prepared for cytomorphometric analysis and immunohistochemistry. Results The nuclei in CGCLs were more numerous, larger, and more irregular than those in PGCLs. Furthermore, CD68 expression and the ratio of CD68+ macrophage to MGCs were significantly greater in CGCLs than in PGCLs. Statistical correlations between CD68 expression and the staining-intensity distribution score within the diagnostic groups were significant in CGCLs and not significant in PGCLs. Conclusion Although the CGCLs share some histopathologic similarities with PGCLs, differences in both nuclear morphometric parameters of MGC and CD68 immunoreactivity may underlie the distinct clinical behavior.
Objective The aim of this study was to investigate the relationship between the immunohistochemical expression of MMP-1 and MMP-9 with the clinical behavior of central giant cell lesions (CGCLs) of ...the jaws. Study design Paraffin-embedded tissue from 30 aggressive and 12 nonaggressive CGCLs was assessed for the expression of MMP-1 and MMP-9 using immunohistochemistry. Results Although cellular immunolocalization patterns of MMP-1 and MMP-9 were similar, mean values of expression estimation/SID scores of each protease were significantly higher in aggressive CGCLs in comparison with nonaggressive lesions. Moreover, linear regression analysis showed that there was a reasonably good correlation not only between the expression estimation but also among SID scores of the 2 proteolytic enzymes. Conclusion The findings of this study suggest a role for MMP-1 and MMP-9 in the resorptive activity of different cellular groups in CGCLs and indicate that differences in immunoreactivity of these 2 proteolytic enzymes may underlie the distinct clinical behavior.
Objective The aim was to examine the relationship between clinicopathological features and immunoexpression of hMLH1 and hMSH2 proteins in pleomorphic adenoma (PA) of minor salivary glands. Study ...design Paraffin-embedded samples of typically benign PA lesions (n = 35) were prepared for histologic and immunohistochemical assessment. Based on the clinicopathologic features, the samples were categorized into low- and high-risk subtypes for their estimated potential for malignant transformation. Results Immunohistochemical analysis revealed strong correlations regarding the expression estimation and staining-intensity distribution (SID) scores between the two proteins. Although there was no relationship between marker immunoexpression and SID scores regarding clinical parameters, statistically significant variations for these parameters were evident regarding some histologic criteria or for risk stratification subtypes. Conclusion The findings of this study could suggest the relationship of a DNA mismatch repair deficiency with high-risk subtypes of PA and the implication of its role in the origin and progression of these subtypes.
