To synthesize highly dispersed Ru nanoparticles (NPs) with desired particle size for NH3 decomposition, an appropriate support is crucial. Herein, spherical mesoporous silica (SMS) NPs with ∼3 nm ...pores are synthesized by a microwave-assisted evaporation-induced self-assembly method and used as support of Ru NPs for NH3 decomposition. Characterization shows that compared to Ru NPs supported on SBA-15 and fumed SiO2, Ru NPs supported on SMS (Ru@SMS) exhibit superior NH3 decomposition performance, achieving a hydrogen generation rate of 31.4 mmol·g-1 cat·min−1 at 500 °C, which is one of the highest reported values for supported Ru catalysts in NH3 decomposition. The excellent NH3 decomposition performance observed on Ru@SMS should be attributed to effective dispersion and appropriate particle size (2.22 ± 0.29 nm) of Ru NPs. This work highlights the potential of SMS as support of Ru catalyst in NH3 decomposition.
•SMS NPs with ∼3 nm pores are synthesized by a microwave-assisted EISA method.•The highly dispersed Ru NPs have mean particle size of 2.22 ± 0.29 nm.•Ru@SMS achieves a hydrogen generation rate of 31.4 mmol·g-1 cat·min−1 at 500 °C.•Ru@SMS shows a marginal decrease in NH3 conversion over a 128-h period.
Moral injury among physicians and other health professionals has attracted attention in the mainstream literature, this study aim to assess the psychometric properties of the 10-item Moral Injury ...Symptoms Scale-Health Professional (MISS-HP) among healthcare professionals in China.
A total of 583 nurses and 2423 physicians were recruited from across mainland China. An online survey was conducted from March 27 to April 26, 2020 (during the middle of the COVID-19 pandemic) using the Chinese version of the MISS-HP. Reliability was assessed by internal consistency reliability and test-retest reliability. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were performed to determine scale structure.
Cronbach's α of the scale for both samples was acceptable (0.71 for nurses and 0.70 for physicians), as was test-retest reliability (ICCs for the individual items ranged from 0.41 to 0.74, with 0.77 for the overall scale in physicians). EFA suggested three factors, and the CFA indicated good fit to the data. Convergent validity was demonstrated with the 4-item Expressions of Moral Injury Scale (r = 0.45 for physicians, r = 0.43 for nurses). Discriminant validity was demonstrated by correlations with burnout and well-being (r = 0.34-0.47), and concurrent validity was suggested by correlations with depression and anxiety symptoms (r = 0.37-0.45). Known groups validity was indicated by a higher score in those exposed to workplace violence (B = 4.16, 95%CI: 3.21-5.10, p < 0.001).
The MISS-HP demonstrated acceptable reliability and validity in a large sample of physicians and nurses in mainland China, supporting its use as a screening measure for moral injury symptoms among increasingly stressed health professionals in this country during the COVID-19 pandemic.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Abstract
In this paper, in order to improve the accuracy of handwritten number recognition, a convolutional neural network based on noise reduction and stack auto coding is formed by combining the ...denoising auto coding algorithm with stack auto coding algorithm. In this paper, the MNIST dataset is preprocessed first, then the noise reduction and stack auto coding network layer are constructed, and finally the stack auto coding algorithm based on the improved denoising auto coding is used to train and test the dataset. Experimental data show that the proposed method achieves low loss value and effectively improves the recognition accuracy in the MNIST test set.
Although many kinds of therapies are applied in the clinic, drug-resistance is a major and unavoidable problem. Another disturbing statistic is the limited number of drug targets, which are presently ...only 20-25% of all protein targets that are currently being studied. Moreover, the focus of current explorations of targets are their enzymatic functions, which ignores the functions from their scaffold moiety. As a promising and appealing technology, PROteolysis TArgeting Chimeras (PROTACs) have attracted great attention both from academia and industry for finding available approaches to solve the above problems. PROTACs regulate protein function by degrading target proteins instead of inhibiting them, providing more sensitivity to drug-resistant targets and a greater chance to affect the nonenzymatic functions. PROTACs have been proven to show better selectivity compared to classic inhibitors. PROTACs can be described as a chemical knockdown approach with rapidity and reversibility, which presents new and different biology compared to other gene editing tools by avoiding misinterpretations that arise from potential genetic compensation and/or spontaneous mutations. PRTOACs have been widely explored throughout the world and have outperformed not only in cancer diseases, but also in immune disorders, viral infections and neurodegenerative diseases. Although PROTACs present a very promising and powerful approach for crossing the hurdles of present drug discovery and tool development in biology, more efforts are needed to gain to get deeper insight into the efficacy and safety of PROTACs in the clinic. More target binders and more E3 ligases applicable for developing PROTACs are waiting for exploration.
Proton production is imperative for many electrochemical reduction reactions, and the ability of an electrocatalyst to facilitate its production is vital for electrocatalysis. Herein commercial RuO2 ...is employed as our electrocatalyst for proton generation during oxygen evolution in a neutral electrolyte (0.2 M Na2SO4). The RuO2 catalyst achieves a low Tafel slope of 93 mV/dec and an overpotential of 570 mV at a current density of 10 mA cm−2. Its mass activity is calculated to be 12.37 A g−1. The Faradaic efficiency of proton generation (FEH+) is found to be 96.8%, 96.3%, 92.5%, 91.3%, and 90.8% at 1.8 V vs. RHE after 1, 3, 6, 9, and 12 h of electrolysis, respectively with a proton yield of 23.75%, 16.41%, 17.10%, 16.78%, and 20.89%, respectively. This study on facilitating proton production will greatly enhance efficiency across myriad energy conversion reactions. The acidified electrolyte can be reused in other applications.
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•Proton production can be realized while inhibiting hydrogen using renewable energy.•Proton is controllably generated during water electrolysis.•The proton concentration is related to the electrocatalytic ability of the electrode.•The proton concentration can be increased to a pH of 1.65 after OER.•A maximum proton yield of 23.75% is obtained after OER.
Mixing molecular building blocks in the solid solution manner is a valuable strategy to obtain structures and properties in between the isostructural parent metal–organic frameworks (MOFs). We report ...nonlinear/synergistic solid‐solution effects using highly related yet non‐isostructural, phosphorescent CuI triazolate frameworks as parent phases. Near the phase boundaries associated with conformational diversity and ligand heterogeneity, the porosity (+150 %) and optical O2 sensitivity (410 times, limit of detection 0.07 ppm) can be drastically improved from the best‐performing parent MOFs and even exceeds the records hold by precious‐metal complexes (3 ppm) and C70 (0.2 ppm).
Beyond mixing: The proper combination of non‐isostructural metal–organic frameworks in terms of solid solutions can drastically improve the porosity (150 %) and optical O2 sensitivity (410 times) compared with the original phases. An oxygen‐sensing MOF (MAF‐2) can undergo structure changes and property improvements when its molecular building blocks are mixed and solid solutions are formed.
As the ship multi-scale phenomenon is very common in visible video, it is an important factor affecting the performance of visible video ship detection. Based on YOLOv5s, this paper proposes a ...real-time multi-scale ship detection algorithm. First, reparameterized convolution is adopted to increase the network width, thereby enhancing the network’s ability to express multi-scale ship features. Second, the depth of SPPF (Spatial Pyramid Pooling Fast) is adjusted to enhance the scale invariance of ship features extracted from the network. Third, the attention module is combined with feature pyramid network to enhance the network’s ability to focus on multi-scale ship features. Finally, confidence propagation cluster is used for post-processing to make the network generation more confident and closer to the boundary box of the real box. The experiment shows that our method can achieve state-of-the-art visible video ship detection performance on multiple evaluation indicators, such as mAP-IOU@0.5, mAP-IOU@0.5:0.95, APS, APM, APL and so on. And it can meet the requirements of real-time detection.
Tea is the world＇s oldest and most popular caffeine-containing beverage with immense economic, medicinal, and cultural importance. Here, we present the first high-quality nucleotide sequence of the ...repeat-rich （80.9%）, 3.02-Gb genome of the cultivated tea tree Camellia sinensis. We show that an extraordinarily large genome size of tea tree is resulted from the slow, steady, and long-term amplification of a few LTR retrotransposon families. In addition to a recent whole-genome duplication event, lineage-specific expansions of genes associated with flavonoid metabolic biosynthesis were discovered, which enhance catechin production, terpene enzyme activation, and stress tolerance, important features for tea flavor and adaptation. We demonstrate an independent and rapid evolution of the tea caffeine synthesis pathway relative to cacao and coffee. A comparative study among 25 Camellia species revealed that higher expression levels of most flavonoid- and caffeinebut not theanine-related genes contribute to the increased production of catechins and caffeine and thus enhance tea-processing suitability and tea quality. These novel findings pave the way for further metabolomic and functional genomic refinement of characteristic biosynthesis pathways and will help develop a more diversified set of tea flavors that would eventually satisfy and attract more tea drinkers worldwide.
Acute lung injury (ALI) is characterized by dysfunction of the alveolar epithelial membrane caused by acute inflammation and tissue injury. Qingwenzhike (QWZK) prescription has been demonstrated to ...be effective against respiratory viral infections in clinical practices, including coronavirus disease 2019 (COVID-19) infection. So far, the chemical compositions, protective effects on ALI, and possible anti-inflammatory mechanisms remain unknown.
In this study, the compositions of QWZK were determined
the linear ion trap/electrostatic field orbital trap tandem high-resolution mass spectrometry (UHPLC-LTQ-Orbitrap MS). To test the protective effects of QWZK on ALI, an ALI model induced by lipopolysaccharide (LPS) in rats was used. The effects of QWZK on the LPS-induced ALI were evaluated by pathological changes and the number and classification of white blood cell (WBC) in bronchoalveolar lavage fluid (BALF). To investigate the possible underlying mechanisms, the contents of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein (MCP-1), interleukin-1β (IL-1β), interleukin-18 (IL-18), and immunoregulatory-related factors interferon-γ (IFN-γ) were detected by ELISA. Furthermore, the expression of Toll-like receptor 4 (TLR4), p-IKKα/β, IKKα, IKKβ, p-IκBα, IκBα, p-NF-κB, nuclear factor-κB (NF-κB), NOD-like receptor family pyrin domain containing 3 (NLRP3), cleaved caspase-1, pro-caspase-1, apoptosis-associated speck-like protein containing CARD (ASC), and β-actin were tested by Western blot.
A total of 99 compounds were identified in QWZK, including 33 flavonoids, 23 phenolic acids, 3 alkaloids, 3 coumarins, 20 triterpenoids, 5 anthraquinones, and 12 others. ALI rats induced by LPS exhibited significant increase in neutrophile, significant decrease in lymphocyte, and evidently thicker alveolar wall than control animals. QWZK reversed the changes in WBC count and alveolar wall to normal level on the model of ALI induced by LPS. ELISA results revealed that QWZK significantly reduced the overexpression of proinflammatory factors IL-6, TNF-α, MCP-1, IL-1β, IL-18, and IFN-γ induced by LPS. Western blot results demonstrated that QWZK significantly downregulated the overexpression of TLR4, p-IKKα/β, p-IκBα, p-NF-κB, NLRP3, cleaved caspase-1, and ASC induced by LPS, which suggested that QWZK inhibited TLR4/NF-κB signaling pathway and NLRP3 inflammasomes.
The chemical compositions of QWZK were first identified. It was demonstrated that QWZK showed protective effects on ALI induced by LPS. The possible underlying mechanisms of QWZK on ALI induced by LPS was
inhibiting TLR4/NF-kB signaling pathway and NLRP3 inflammasome activation. This work suggested that QWZK is a potential therapeutic candidate for the treatments of ALI and pulmonary inflammation.
Fibronectin type III domain-containing 5 (FNDC5) protein induces browning of subcutaneous fat and mediates the beneficial effects of exercise on metabolism. However, whether FNDC5 is associated with ...hepatic steatosis, autophagy, fatty acid oxidation (FAO), and lipogenesis remains unknown. Herein, we show the roles and mechanisms of FNDC5 in hepatic steatosis, autophagy, and lipid metabolism. Fasted FNDC5
mice exhibited severe steatosis, reduced autophagy, and FAO, and enhanced lipogenesis in the liver compared with wild-type mice. Energy deprivation-induced autophagy, FAO, and AMPK activity were attenuated in FNDC5
hepatocytes, which were restored by activating AMPK with 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR). Inhibition of mammalian target of rapamycin (mTOR) complex 1 with rapamycin enhanced autophagy and FAO and attenuated lipogenesis and steatosis in FNDC5
livers. FNDC5 deficiency exacerbated hyperlipemia, hepatic FAO and autophagy impairment, hepatic lipogenesis, and lipid accumulation in obese mice. Exogenous FNDC5 stimulated autophagy and FAO gene expression in hepatocytes and repaired the attenuated autophagy and palmitate-induced steatosis in FNDC5
hepatocytes. FNDC5 overexpression prevented hyperlipemia, hepatic FAO and autophagy impairment, hepatic lipogenesis, and lipid accumulation in obese mice. These results indicate that FNDC5 deficiency impairs autophagy and FAO and enhances lipogenesis via the AMPK/mTOR pathway. FNDC5 deficiency aggravates whereas FNDC5 overexpression prevents the HFD-induced hyperlipemia, hepatic lipid accumulation, and impaired FAO and autophagy in the liver.
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