هدفت الدراسة إلى تقييم فعالية مغلي أوراق الشجرة المعروفة بالمورينجا في إزالة الطبقة السميكة مقارنة بمادتين أخريين وكذلك الأنشطة المضادة للميكروبات.
تم استخراج أوراق الشجرة باستخدام مغلي الماء الساخن ...بتركيزين مختلفين. تم تحضير ما مجموعه ثلاثين سنا بشريا مستخرجا ذو جذر واحد لتقييم فعالية إزالة الطبقة السميكة. تم حساب وجود الطبقة السميكة في الثلث الأوسط من قناة الجذر باستخدام الميكروسكوب الليزري الماسح الضوئي الحويصلي. ثم تم إجراء النشاط المضاد للبكتيريا على نوعين من البكتيريا باستخدام طريقة تشتت الأغار.
وجدنا أن التراكيز 2,5% و 5% من المغلي أكثر فعالية بشكل ملحوظ في إزالة الطبقة السميكة، ومع ذلك، لم يتم ملاحظة فرق ملحوظ عند المقارنة مع المادتين الأخريين. أظهرت نتائج اختبار المضادات الحيوية المختبرية أن خمسة بالمائة من المغلي يظهر نشاطا مضادا للميكروبات أعلى ضد كلا المسارين التجريبيين.
تشير هذه النتائج إلى أن مغلي أوراق الشجرة يمكن اعتباره محلولا فعالا في علاج الجذور.
This study evaluated the effectiveness of moringa (Moringa oleifera) leaves decoction for removing a smear layer compared to sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic acid (EDTA), as well as its antimicrobial activities.
The moringa leaves were extracted using hot water decoction at two different concentrations (2.5% and 5.0% w/v). A total of 30 extracted human single-rooted teeth were prepared to assess the smear layer removal efficacy. The presence of a smear layer in the middle third of the root canal was detected by confocal microscopy. Then the antibacterial effects were assessed against Enterococcus faecalis and Streptococcus mutans bacteria using the agar diffusion method.
The 2.5% and 5.0% decoction were significantly more effective than 0.25% NaOCl in removing the smear layer (p < 0.05); however, no significant difference was observed compared to EDTA (p > 0.05). The in vitro antimicrobial assay showed that 5.0% decoction had higher antimicrobial activity against both of the test pathogens.
The findings of this study suggest that moringa leaves decoction can be considered an effective irrigant in endodontics.
Received date: 10 October 2018 Accept date: 19 November 2018 Introduction According to the World Health Organization (WHO), dental caries are a major health problem worldwide with 60-90% prevalence ...in school-aged children and adults.1,2 Caries are a common oral disease and result in loss of tooth structure and requires restoration to restore the function, form and aesthetic of the tooth.3-5 Composite resins are the most widely used restorative materials today. ...the tooth undergoes finishing and polishing (Figure 5). ...the natural anatomy of the existing tooth is lost.17,18 Restoration of the composite resin in the posterior tooth is a challenge for the dentist because it requires recontouring of anatomical tooth surfaces. Gaps occur when the composite polymerization pressure is greater than the composite bond strength with the tooth structure.10,19,20 Several methods for reducing polymerization shrinkage have been reported in posterior restorations, such as the use of liner materials with a low elastic modulus, techniques of curing, and application technique of composite resin material.19,20 Incremental technique has been reported to reduce microleakage with resin composite restoration.
Objective: To evaluate the microhardness and porosity of the dentin of Wistar rats after pulp-out application.
Materials and Methods: This was laboratory experimental research with posttest design ...with control group. Twelve teeth of wistar rats were allocated into 2 groups, the test group and the control group. In the test group, teeth were prepared on the labial surface until half thickness of the dentin, then pulpout was applied to the base of cavity, covered with GIC. On the 7th day, euthanasia and extraction were performed to test the dentin microhardness with Vickers Hardness Tester and dentin porosity with Laser Scanning Microscope (LSM).
Results: The mean value of tooth hardness after pulp-out application was 22.23 ± 3.51 HV while the mean value of the control group was 27.70 ± 1.70 HV, with p value = 0.31 at 95% significance level. There was a decrease in tooth hardness of treated sample which was not significantly different compared to the control group. CLSM photo shows white lines on the sample surface as the exposed dentinal tubules with a higher intensity compared to the control group.
Conclusion: Pulp-out decreased tooth microhardness and cause porosity of the teeth.
Enterococcus faecalis is a coccal Gram-positive bacteria with facultative anaerob feature. NaOCl have been proven effective againts Enterococcus faecalis for root canal irrigation process. Green tea ...leaf extract (Camellia sinensis) can be chosen as an alternative solution for root canal irrigation. The purpose of this study is to determine the difference of green tea leaf extract (Camellia sinensis) and NaOCl 2.5% efficacy againts the growth of Enterococcus faecalis bacteria. This is a laboratory experimental study with “post test only group” design. The first step of the trial was done by making green tea leaf extract and then determine the lowest concentration on which the first solution become clear. The concentration that were tested are 1.5%, 2.5%, 3.5%, 4.5% and 5.5%. Based on the trial, it was found that the minimal inhibiton concentration of the green tea leaf extract was 1.5%. The anti-bacterial effect testing method was using diffusion method to differenciate inhibition zone of the green tea leaf extract solution on various concentrations that were being tested and compare it with NaOCl 2.5%. Each group was replicated three times. The obtained data was analyzed with One Way Anova test and the continued with LSD test. The result of the study is that NaOCl 2.5% have superior anti-bacterial effect againts Enterococcus faecalis compared to green tea leaf extract.
This study aimed to compare the ability of the three antioxidant ingredients and a minimal application to decrease microleakage of composite resin restoration after intracoronal bleaching. Fifty ...maxillary first incisor that met the inclusion criteria were prepared endodontic treatment. They are divided into 10 groups: control group (no antioxidant), sodium ascorbate 10%, catalase 10% and sodium ascorbate 10% with Tween 80 0.2% and an application period of 1 hour, 24 hours and 48 hours. Applied hydrogen peroxide 35% for 5 days. Samples were restored with composite resin and coated with nail polish, placed China ink for 24 hours. Coronal microleakage was assessed using a stereomicroscope, which showed presented a significant different (ρ<0.05) in 48 hours. It was concluded that sodium ascorbate 10% with Tween 80 0.2% had a significant effect in decreasing coronal microleakage in 48 hours.
This study aimed to compare apical microleakage using tooth clearing method between two softened core obturation techniques which are carrier based gutta percha and continuous wave. Samples are ...maxillary first incisors, with a single root canal. They are devided into three groups of 9 each randomly. The first group is carrier based gutta percha, the second is continuous wave, and the third is lateral condensation as positive control. The teeth were decoronated at the cementoenamel junction, prepared with crown down pressureless technique, and obturated according their group. All the samples were coated with nail polish except for 2 mm from the apical, and then placed in China Ink for 7 days. After that, the polish were removed and the samples undergone tooth clearing procedure. When the samples had been transparant, they were observed using microscop. Data were collected and analyzed using Anova and Tukey’s post hoc test. The study showed that mean of apical microleakage from continuous wave technique (0.256+0.133) is less than from carrier based gutta percha (0.433+0.173). As the conclusion, there is a significant diffrence (p:0.034) of apical microleakage between continuous wave and carrier based gutta percha (p<0.05).
The aim of this research was to determine the microleakage apical sealer based on epoxy resin and methacrylate resin with continuous wave obturation technique.Thirty permanent lateral incisors were ...selected at random and divided into 3 groups (N=10), namely AH Plus obturator group (epoxy resin), EndoREZ group (methacrylate resin), and negative control. The samples were decoronated, root canal preparation, then kept in incubator of 37°C for 72 hours. Sample were coated with nail varnish then immersed in methylene blue for 48 hours. The samples were washed with distilled water, dried and nail varnish removed. The samples were clearing. Penetration was measured using microscope and given score 0-4. Measurements were analyzed statistically. By using Kruskal Wallis test and Mann Whitney test, there is no significant difference between the apical microleakage of the the epoxy resin root canal sealer with methacrylate root canal sealer based (p>0,05). It means that the apical microleakage of the epoxy resin root canal sealer based comparable with merhacrylate root canal sealer based. It was concluded that the apical microleakage of epoxy resin based sealer does not different to the methacrylate resin based sealer
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