The status of anti-influenza immunity in workers contacting with chemical substances having sensitizing properties was studied. Workers immunized with trivalent anti-influenza vaccine were found to ...have no expected increase in antibody titers. These findings were indicative of inadequate response and the impairment of nonspecific protection in the immunized workers. Such alterations of immune response make it necessary to carry out immunization with the control of immunological characteristics and the simultaneous administration of immunocorrective therapy.
In limited observations made on schoolchildren aged 7-14 years, the variant of adsorbed chemical influenza vaccine, intended for children, has been shown to possess low reactogenicity, high antigenic ...potency and protective effectiveness.
The principal possibility of isolation of internal proteins (M and NP) of influenza type A (H1N1 and H3N2) and B viruses by SDS-PAG preparative electrophoresis and preparation of monospecific ...antisera to these proteins was demonstrated. The resulting preparations may be used for testing the biological objects by enzymeimmunoassay.
A test system for the detection and quantitation of influenza virus hemagglutinin (HA) in biological specimens by solid-phase enzyme immunoassay (SPEIA) is described. The test system implies ...sequential adsorption on polystyrene base of anti-HA guinea pig gammaglobulins, detectable HA-containing antigen, monospecific anti-HA rabbit serum, and peroxidase antirabbit conjugate. Adsorption of the HA-containing antigen is run in the presence of a high concentration of nonionic detergent. The developed method is highly sensitive (0.3-0.5 ng in a specimen) and permits the detection and quantitation of HA in whole influenza virions, preparations of surface glycoproteins and in preparations of hemagglutinin recovered from virus particles. The analysis (after pre-sensitization of the polystyrene adsorbent) takes from 2 to 21/2 hours. The possibility of using the developed test system for control and standardization of inactivated influenza vaccines (whole-virion, split, and subunit) is discussed.
The use of affinity column sepharose-tyrosine-sulfanilic acid permits one to obtain preparative amounts of pure hemagglutinin of the types H1, H2, H3 from the total amount of surface glycoproteins ...solubilized by octylglycoside from antigenically different strains of influenza A virus. The yield of hemagglutinin ranges from 30% to 84% depending on the virus strain.
Adsorbed chemical influenza vaccine is a standard preparation. It meets with the WHO requirements with respect to the content of hemagglutinin, ovalbumin, protein nitrogen. For the dosage of the ...vaccine by the hemagglutinin content in weight units (microgram) in the process of manufacture, the development of the national standard of this antigen is necessary. After the treatment of virus suspension with ether the number of intact virions remains stable, constituting 3-4%.
