The effectiveness of the increase in passive ventilation was studied as remedial action for high concentrations of indoor radon gas activity. The rationale has been to apply the provisions of ...Directive 2013/59/EURATOM, partially implemented by a regional law of Puglia Region (Southern Italy). Residential buildings were selected based on characteristics common to those of commercial premises, which are the recipients of legislative obligations. The final goal of this study was to evaluate the feasibility of carrying out the same remediation activity for both types of buildings. From a previous survey in Puglia in 2013–2014, 18 houses were selected and passive ventilation strategy was applied in order to evaluate its effect on the radon activity concentration. A specially designed protocol defined frequency and time of windows operation. The average value of the annual measurement revealed a significant reduction of the internal radon activity concentration: mitigation ranged from 21 ± 5% to 58 ± 2% with an average value of 33 ± 3%. The results of the study showed that passive ventilation is an effective method of mitigation of the internal radon concentration if correctly designed according to the local characteristics of the buildings and habits of the occupants.
The structural and mechanical properties of the microenvironmental context have a profound impact on cancer cell motility, tumor invasion, and metastasis formation. In fact, cells react to their ...mechanical environment modulating their adhesion, cytoskeleton organization, changes of shape, and, consequently, the dynamics of their motility. In order to elucidate the role of extracellular matrix stiffness as a driving force in cancer cell motility/invasion and the effects of ionizing radiations on these processes, we evaluated adhesion and migration as biophysical properties of two different mammary cell lines, over a range of pathophysiological stiffness (1-13 kPa) in a control condition and after the exposure to two different X-ray doses (2 and 10 Gy, photon beams). We concluded that the microenvironment mimicking the normal mechanics of healthy tissue has a radioprotective role on both cell lines, preventing cell motility and invasion. Supraphysiological extracellular matrix stiffness promoted tumor cell motility instead, but also had a normalizing effect on the response to radiation of tumor cells, lowering their migratory capability. This work lays the foundation for exploiting the extracellular matrix-mediated mechanism underlying the response of healthy and tumor cells to radiation treatments and opens new frontiers in the diagnostic and therapeutic use of radiotherapy.
Radon, a radioactive inert gas that comes from the decay of naturally occurring radioactive species, poses a substantial health risk due to its involvement in lung cancer carcinogenesis. This work ...proposes a metrological approach for determining radon exhalation rates from diverse building materials. This methodology employs an electrostatic collection chamber for alpha spectrometry of radon isotopic decay products. Experimental evaluations were conducted particularly focusing on volcanic gray tuff from Sant’Agata de’ Goti (Campania region, Italy), a material commonly utilized in construction, to assess radon exhalation rates. The study aligns with Legislative Decree 101/2020, a transposition of European Directive 59/2013/Euratom, highlighting the need to identify materials with a high risk of radon exhalation. Moreover, this work supports the goals of the Italian National Radon Action Plan related to the aforementioned decree, aiming to develop methodologies for estimating radon exhalation rates from building materials and improving radioprotection practices.
The biological effects of ionizing radiation are exploited in the clinical practice of radiotherapy to destroy tumour cells while sparing the surrounding normal tissue. While most of the radiotherapy ...research focused on DNA damage and repair, recently a great attention is going to cells' interactions with the mechanical microenvironment of both malignant and healthy tissues after exposure. In fact, the stiffness of the extracellular matrix can modify cells' motility and spreading through the modulation of transmembrane proteins and surface receptors' expression, such as CD-44. CD-44 receptor has held much interest also in targeted-therapy due to its affinity with hyaluronic acid, which can be used to functionalize biodegradable nanoparticles loaded with chemotherapy drugs for targeted therapy. We evaluated changes in CD-44 expression in two mammary carcinoma cell lines (MCF10A and MDA-MB-231) after exposure to X-ray (2 or 10 Gy). To explore the role of the mechanical microenvironment, we mimicked tissues' stiffness with polyacrylamide's substrates producing two different elastic modulus values (0.5 and 15 kPa). We measured a dose dependent increase in CD-44 relative expression in tumour cells cultured in a stiffer microenvironment. These findings highlight a crucial connection between the mechanical properties of the cell's surroundings and the post-radiotherapy expression of surface receptors.
For the first time, a map of the
222
Rn gas has been produced in the Campania region, the southern part of Italy, based on the activity concentration measured in indoor environments. This work is ...part of the radon mitigation policy and complies with the recent Italian Legislative Decree 101/2020, which implements the European Basic Safety Standards, Euratom Directive 59/2013, where Member States must declare areas with elevated levels of indoor radon concentration. The obtained map, divided by Campania municipalities, identifies priority areas with activity concentration values exceeding the reference level of 300 Bq m
-3
. In addition, an effective statistical analysis of the dataset has been carried out.
Surface brachytherapy (BT) lacks standard quality assurance (QA) protocols. Commercially available treatment planning systems (TPSs) are based on a dose calculation formalism that assumes the patient ...is made of water, resulting in potential deviations between planned and delivered doses. Here, a method for treatment plan verification for skin surface BT is reported. Chips of thermoluminescent dosimeters (TLDs) were used for dose point measurements. High-dose-rate treatments were simulated and delivered through a custom-flap applicator provided with four fixed catheters to guide the Iridium-192 (Ir-192) source by way of a remote afterloading system. A flat water-equivalent phantom was used to simulate patient skin. Elekta TPS Oncentra Brachy was used for planning. TLDs were calibrated to Ir-192 through an indirect method of linear interpolation between calibration factors (CFs) measured for 250 kV X-rays, Cesium-137, and Cobalt-60. Subsequently, plans were designed and delivered to test the reproducibility of the irradiation set-up and to make comparisons between planned and delivered dose. The obtained CF for Ir-192 was (4.96 ± 0.25) μC/Gy. Deviations between measured and TPS calculated doses for multi-catheter treatment configuration ranged from -8.4% to 13.3% with an average of 0.6%. TLDs could be included in clinical practice for QA in skin BT with a customized flap applicator.
This work is focused on effectiveness of adopting the Plan, Do, Check, Act (PDCA) cycle model, also known as Deming cycle, for an analytical laboratory management. It is not uncommon for university ...laboratories, unlike those of private companies, to register problems and losses in the management of materials, supplies and quality of service, especially externally. The PDCA cycle is closely associated with the planning, implementation, control and continuous improvement of the processes. In this context, the Deming cycle method finds a simple and informal application in order to make small modifications and improvements to the entire Radon gas activity concentrations measurement procedure. Attention is paid to most important topics as management of equipment and materials, record maintenance, sample analysis and risk communication. The results recorded were positive in terms of material savings, job optimization, quality of results and organization of internal processes.
Abstract
Tuscany, with its mixed geology, offers a great variety of stones that are used to build entire villages. In this paper, two of these building materials are considered:
Rosso Ammonitco
and
...Pietra Serena.
In particular, the activity concentration of
226
Ra,
232
Th and
40
K have been analysed in order to estimate the value of the gamma index. In addition to this, the X-ray fluorescence spectroscopy has been carried out to obtain a chemical characterization of the stones. Although these kind of stones are not subjected to radiological characterization, the approach used was the one reported in Radiation Protection 112, also implemented in the Italian Legislative Decree 101/2020, because of their lithographical peculiarity and their employment in construction.
In this paper, an in-depth and systematic study of the radiological characterization of three types of Puglia region natural limestones (Pietra Leccese, Pietra Mazzara and Carparo) was carried out. ...The investigation was performed by XRF spectroscopy for a chemical analysis, and gamma spectroscopy of the specific activity concentration of natural radionuclides 226Ra, 232Th, and 40K. Although the limestone does not fall within the category included by Italian Legislative Decree 101/2020, the gamma index was calculated using the results of the gamma spectroscopy measurements. For Pietra Mazzara and Carparo stones, the gamma index was found to be less than the reference value; conversely Pietra Leccese was found to be higher. To obtain a more complete evaluation of the external exposure, radium equivalent activity and external radiation hazard were calculated for all analyzed stones. The results suggest the need to broadly consider the radiological risk for these stones, and for limestone more generally, when used as a building material.
Outreach projects are often used to a limited extent for dissemination purposes and rarely have a significant impact on the student’s teaching and technical skills. The RadioLab project requires a ...proactive interaction between researchers and students by experimental activities for measuring environmental radioactivity, in particular radon gas. Buildings considered to be of radiological interest, such as schools, have been selected to carry out radon gas activity concentration measurements using solid-state nuclear track passive detectors LR-115. The results of annual measurements, made over 6 years and involving a total of 952 rooms, distributed in 67 schools throughout the Campania region, were collected. These data, deemed scientifically reliable (i) can be overlapped over geological characterization data enhancing the relationship between lithology and radon, (ii) confirmed data from the radon potential map of the Campania region about the distribution of indoor radon, and finally (iii) contributed to the collection of radon indoor data of the Campania region. The results obtained highlighted the need and effectiveness of increasing the network of schools involved in the outreach activity and in the implementation of experimental activities with applicative effects in the scientific and research sectors.
PDF
