The unique genetic makeup and remarkable competency of Vibrio cholerae are the key factors that help the cholera pathogen adapt rapidly to adverse environmental conditions and resist the detrimental ...effect of antimicrobial agents. In the last few decades, V. cholerae that causes acute watery diarrhoeal disease cholera has emerged as a notorious multidrug resistant (MDR) enteric pathogen. Although chromosomal mutations can contribute to antimicrobial resistance (AMR), the frequent acquisition of extrachromosomal mobile genetic elements (MGEs) from closely/distantly related bacterial species are major players in V. cholerae drug resistance. Whole genome sequence analysis of clinical and environmental V. cholerae strains revealed that the genome of most of the recent isolates harbour integrating conjugative elements (ICEs), plasmids, superintegron, transposable elements and insertion sequences, which are the key carriers of genetic traits encoding antimicrobial resistance function. Different antimicrobial resistance genes identified in V. cholerae can contribute in antibiotic resistance by facilitating one of the following three mechanisms; (i) reduced permeability or active efflux of the antibiotics, (ii) alteration of the antibiotic targets by introducing post-transcriptional/translational modifications and (iii) hydrolysis or chemical modification of antibiotics. Here, we present an overview of the present insights on the emergence and mechanisms of AMR in V. cholerae.
Nowadays, in a world full of uncertainties and the threat of digital and cyber-attacks, blockchain technology is one of the major critical developments playing a vital role in the creative ...professional world. Along with energy, finance, governance, etc., the healthcare sector is one of the most prominent areas where blockchain technology is being used. We all are aware that data constitute our wealth and our currency; vulnerability and security become even more significant and a vital point of concern for healthcare. Recent cyberattacks have raised the questions of planning, requirement, and implementation to develop more cyber-secure models. This paper is based on a blockchain that classifies network participants into clusters and preserves a single copy of the blockchain for every cluster. The paper introduces a novel blockchain mechanism for secure healthcare sector data management, which reduces the communicational and computational overhead costs compared to the existing bitcoin network and the lightweight blockchain architecture. The paper also discusses how the proposed design can be utilized to address the recognized threats. The experimental results show that, as the number of nodes rises, the suggested architecture speeds up ledger updates by 63% and reduces network traffic by 10 times.
The current study gives more information on Lepidoptera (butterfly) diversity and their food plant resources in the conifer woods of Shimla Water Catchment Wildlife Sanctuary, Himachal Pradesh. The ...study was carried out during the three distinct seasons; summer, monsoon, and autumn from March 2019–April 2022. A total of 1650 butterflies, representing 68 species and 6 families were recorded. The Nymphalidae comprised the greatest number of species (28 species), followed by the Lycaenidae (15), Pieridae (13), Papilionidae (7), Hespiradie (4) and Riodinidae (1). Species abundance and richness were more in mixed forest type which owing water and grass-cover availability supported most unique butterfly assemblages as compared to pure conifers and oak forest. The months from April to November harbor the greatest abundance of species. An overall reduction in species abundance was seen from December through January and until the end of March. This study provides an understanding of butterflies and has inspired additional investigation for the restoration of forest habitats in this protected area. The current study on diversity shows the importance of preserving biodiversity and monitoring climate change. It offers a basic identification, gathers data from a comparative viewpoint, allows synthesis, and develops and stimulates ideas and hypotheses that are applicable to other fields.
Hematopoietic stem cells (HSCs) possess two important properties such as self-renewal and differentiation. These properties of HSCs are maintained through hematopoiesis. This process gives rise to ...two subpopulations, long-term and short-term HSCs, which have become a popular convention for treating various hematological disorders. The clinical application of HSCs is bone marrow transplant in patients with aplastic anemia, congenital neutropenia, sickle cell anemia, thalassemia, or replacement of damaged bone marrow in case of chemotherapy. The self-renewal attribute of HSCs ensures long-term hematopoiesis post-transplantation. However, HSCs need to be infused in large numbers to reach their target site and meet the demands since they lose their self-renewal capacity after a few passages. Therefore, a more in-depth understanding of
HSCs expansion needs to be developed to delineate ways to enhance the self-renewability of isolated HSCs. The multifaceted self-renewal process is regulated by factors, including transcription factors, miRNAs, and the bone marrow niche. A developed classical hierarchical model that outlines the hematopoiesis in a lineage-specific manner through
fate mapping, barcoding, and determination of self-renewal regulatory factors are still to be explored in more detail. Thus, an in-depth study of the self-renewal property of HSCs is essentially required to be utilized for
expansion. This review primarily focuses on the Hematopoietic stem cell self-renewal pathway and evaluates the regulatory molecular factors involved in considering a targeted clinical approach in numerous malignancies and outlining gaps in the current knowledge.
Filarial parasites modulate effective immune response of their host by releasing a variety of immunomodulatory molecules, which help in the long persistence of the parasite within the host. The ...present study was aimed to characterize an immunomodulatory protein of Brugia malayi and its interaction with the host immune component at the structural and functional level. Our findings showed that Brugia malayi Calreticulin (BmCRT) is responsible for the prevention of classical complement pathway activation via its interaction with the first component C1q of the human host. This was confirmed by inhibition of C1q dependent lysis of immunoglobulin-sensitized Red Blood Cells (S-RBCs). This is possibly the first report which predicts CRT-C1q interaction on the structural content of proteins to explain how BmCRT inhibits this pathway. The molecular docking of BmCRT-C1q complex indicated that C1qB chain (IgG/M and CRP binding sites on C1q) played a major role in the interaction with conserved and non-conserved regions of N and P domain of BmCRT. Out of 37 amino acids of BmCRT involved in the interaction, nine amino acids (Pro(126), Glu(132), His(147), Arg(151), His(153), Met(154), Lys(156), Ala(196) and Lys(212)) are absent in human CRT. Both ELISA and in silico analysis showed the significant role of Ca(+2) in BmCRT-HuC1q complex formation and deactivation of C1r2-C1s2. Molecular dynamics studies of BmCRT-HuC1q complex showed a deviation from ∼ 0.4 nm to ∼ 1.0 nm. CD analyses indicated that BmCRT is composed of 49.6% α helix, 9.6% β sheet and 43.6% random coil. These findings provided valuable information on the architecture and chemistry of BmCRT-C1q interaction and supported the hypothesis that BmCRT binds with huC1q at their targets (IgG/M, CRP) binding sites. This interaction enables the parasite to interfere with the initial stage of host complement activation, which might be helpful in parasites establishment. These results might be utilized for help in blocking the C1q/CRT interaction and preventing parasite infection.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The formal and the informal market are two significant parts of the economy in India. However, the portion of the informal sector is more when contrasted with the formal one. The informal share of ...market, which eventually bolsters the conventional economy, is expanding step by step. These informal Markets give a special topic of sociological examination with regards to the informal economy. This paper intends to sociologically contextualize street vendors and weekly markets in the triangle shaped by the urban space, informal economy and state, and see how informalness is conjured in formal metropolitan circle. This examination through different past investigations attempted to comprehend vendors profile of these market and their conduct with shoppers and among themselves. This paper investigates the profile of consumer and their conduct in urban weekly market. The conduct that the consumers show in looking for, buying, utilizing, assessing and discarding items and services that they expect will fulfil their necessities. Consumer behaviour is impacted by different components like individual, ecological and dynamic. This examination utilizes distinctive other recently distributed attempts to comprehend the idea of weekly market. Further this article also brings into light various problems faced by these informal markets and also talks about policies and legislation brought by government for their betterment.
Aluminium is considered a green metal due to its environmental responsive characteristics. The 7475-T7351 aluminum alloy is extensively used in automotive and aerospace applications due to its light ...weight and high strength. In the present work, the effects of the corrosive environment on the high cycle fatigue (HCF) behaviors of the 7475-T7351 aluminum alloy was investigated. The aqueous solution of sodium chloride was used for solution treatment. The HCF test was performed on pre-cracked specimens using a servo-hydraulic universal testing machine, Instron 8800. The fractured specimens were characterized using a scanning electron microscope. It was observed that the crack propagation occurred through anodic dissolution at high stress and a significant crack tip blunting and crack extension occurred. However, no appreciable change in crack growth was noticed over the lower frequency range of 0.1 to 0.9 Hz. The slower growth rate envisages oxide debris formation between the cracked faces. When the alloy was treated under corrosive environments, the HCF tests depicted that the fatigue life reduces up to two orders of magnitude. The corrosion pits induced the crack initiation in stage-I at lower alternating stress; however, the fatigue crack growth rate (FCGR) was increased in the corrosive environment. The transition from stage-I to stage-II occurred at a lower stress intensity range (∆ K ) level; it was due to the combined effects of corrosion, hydrogen embrittlement, active path dissolution, and stress concentration. The corrosion fatigue test at low frequency also depicted a slower FCGR as compared to its moderate frequency counterpart and showed an irregular crack growth behavior.
The present study was aimed to determine the total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of plasma and renal tissue in cisplatin (cDDP) induced ...nephrotoxic rats and its protection by treatments with floral extracts of Calendula officinalis Linn. Treatment with cDDP elevated (p < 0.05) the levels of blood urea nitrogen, creatinine (CR), TOS, OSI and malondialdehyde (MDA) but lowered (p < 0.05) total plasma proteins, TAS, total thiols (TTH), blood glutathione (GSH) and antioxidant enzymes compared to the control group. Pre- and post-treatments of ethanolic floral extract of C. officinalis along with cDDP restored (p > 0.05) CR, albumin, TOS, GSH and activities of antioxidant enzymes in blood and renal tissue. Ethanolic extract treatments reduced (p < 0.05) MDA level in renal tissue without restoring the erythrocyte MDA level following cDDP treatment. These observations were further supported by the histopathological findings in renal tissue. Observations of the present study have shown that treatments with ethanolic floral extract of C. officinalis protect cDDP induced nephrotoxicity by restoring antioxidant system of the renal tissue.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Cloud computing offers dynamic, scalable, and virtualized computing resources to end users over the internet. Load balancing is crucial for efficient resource use, distributing workloads across ...multiple resources to prevent overloading. Load balancing is crucial for resource utilization and processing time reduction, but traditional algorithms are often stuck at local maxima, leading to unequal allocation and performance decline. A metaheuristic based algorithm is proposed to dynamically adjust load distribution, ensuring resilience and sensitivity to changing workloads while managing energy consumption. This research presents a Rock Hyrax-based load balancing algorithm that addresses local maxima and power efficiency issues using QoS parameters. The algorithm's performance is evaluated qualitatively and statistically, considering both static and dynamic modes of jobs and virtual machines. Comparing it with existing scheduling algorithms, the algorithm reduces makespan by 10%-15% and total energy consumption in data centers by 8%-13%. These results demonstrate the effectiveness of the Rock Hyrax-based load balancing algorithm in improving performance and energy efficiency in data centers, highlighting its potential impact on optimizing resource allocation and enhancing overall system performance.
To assess the accuracy of freehand cervical C1 C2 screws placement by knock and drill (K and D) technique in craniovertebral anomalous bony anatomy.
From January 2017 to December 2022, 682 ...consecutive C1 C2 screws in 215 patients with craniovertebral junction (CVJ) anomalies were enrolled. All patients underwent posterior fixation with K and D technique without any fluoroscopic guidance. The patient's demographic details, clinical details, radiological details, major intraoperative events, and postoperative complications were noted. The screws malposition grades and direction on CT images in the axial and sagittal plane were defined as new per proposed "SGPGI accuracy criteria." All patients had a clinical evaluation at 3-month follow-up.
Total 682 C1, C2 screws were placed in 215 patients for CVJ anomalies using K and D technique. The accuracy of screws placement by freehand technique was 84.46% (576/682). So with technique explained the rate of malplacement in simple (16.35%) and complex (15.19%) groups were almost comparable and comparison difference was not significant (
= 0.7005).
The freehand technique, as described, is effective in cases of anomalous bony anatomy, and it is mandatory in complex CVJ anomalies. The accuracy of screw placement and VA injury is comparable with major studies. This technique is supposedly cost-effective and less hazardous to both health-care workers and patients.
PDF
