Numenius is an author who straddles the line between Middle Platonism and Neoplatonism. In this contribution, I focus on the differences between the second and the third God, which emerge from ...analyses of the relevant fragments. Numenius emphasizes, on several occasions, how the second God (i.e., the demiurge) has a dual nature. In this paper, I investigate the role of the demiurge in Numenius and examine in what sense the second and third God are “one.” On the one hand, Numenius seems to be stressing the unity of the second and third levels of reality, but on the other hand, he also appears to be differentiating them. The present analyses concentrate on fragments 19F, 24F, 29T and 30T (respectively 11, 16, 21, and 22 in des Places’ edition). My purpose is to demonstrate that, according to Numenius, the second and the third God are one because they both can be regarded as demiurgic. Thus, Numenius conceives a kind of “double demiurgy,” which preserves the distinction between the second and the third God, who are distinguished from an ontological point of view, but who, at the same time, share a demiurgic function. The second God is then the paradigm, whereas the third God is immanent in matter as a ruling principle of the cosmos, which is similar to the World Soul, as he operates on matter in order to make it rationally ordered.
Nervous Necrosis Virus (NNV) represents one of the most threatening pathogens for Mediterranean aquaculture. Several NNV strains are currently co-circulating in the Mediterranean Basin with a high ...prevalence of the RGNNV genotype and the RGNNV/SJNNV reassortant strain and a more limited diffusion of the SJNNV genotype and the SJNNV/RGNNV reassortant. In the present study, a one-step multiplex RT-PCR (mRT-PCR) assay was developed as an easy, cost-effective and rapid diagnostic technique to detect RGNNV and the reassortant RGNNV/SJNNV strain and to distinguish them from SJNNV and the reassortant SJNNV/RGNNV strain in a single RT-PCR reaction. A unique amplification profile was obtained for each genotype/reassortant enabling their rapid identification from cell culture lysates or directly from brain tissues of suspected fish. The method’s detection limit varied between 10
2.3
and 10
3.4
TCID
50
ml
-1
depending on viral strains. No cross-reacitivty with viruses and bacteria frequently associated with gilthead seabream, European seabass and marine environment was observed. The mRT-PCR was shown to be an accurate, rapid and affordable method to support traditional diagnostic techniques in the diagnosis of VNN, being able to reduce considerably the time to identify the viral genotype or the involvement of reassortant strains.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Numenius is one of the most important authors who, in the Imperial Age, deal with the figure of Socrates. Socrates is important in the Platonic tradition, in particular in the sceptical tradition, ...when the Socratic dubitative “spirit” of the first Platonic dialogues became important to justify the “suspension of judgement.” Numenius criticises the whole Academic tradition by saying that the Academics (particularly the sceptics) betrayed the original doctrine of Plato and formulated a new image of Socrates. For Numenius, Socrates plays a central role because Plato would have inherited his doctrine. What does Socrates’s doctrine consist in? According to Numenius, Socrates theorised a “doctrine of three Gods” (which can be likely found in the second Platonic epistle) which is strictly bound up with the main aspect of Plato’s thought. In fact, in Numenius’s view, Plato belongs to a genealogy which can be linked to Pythagoras himself. From this perspective, Numenius says that Socrates’s original thought is a theology which also belongs to the Pythagorean tradition and which Plato further developed. For Numenius, Socrates is not the philosopher of doubt, but a theologian who first theorised the existence of three levels of reality (Gods), which is also the kernel of Numenius’s metaphysical system. For this reason, Numenius puts Socrates within a theological genealogy that begins with Pythagoras and continues with Socrates and Plato, and that the Academics and the Socratics failed to understand.
Numenio appartiene all’ambito degli interpreti medioplatonici dei dialoghi di Platone; nel Medioplatonismo, infatti, si diffuse la tendenza a interpretare la filosofia di Platone come in chiave ...sistematica. Il dialogo più rilevante per questa operazione esegetica nel Medioplatonismo fu certamente il Timeo. In questo contributo intendo concentrarmi sull’interpretazione delle pagine 28a-29a del Timeo da parte di Numenio di Apamea ed esaminarne l’esegesi alla luce della sua prospettiva onto-teologica. Nel Timeo, l’utilizzo del verbo βλέπω ha la funzione di descrivere il fatto che il demiurgo è contemplativo nei confronti del paradigma intelligibile. In Numenio, invece, l’associazione del verbo βλέπω al demiurgo è volta a mostrare il ruolo di mediatore che il secondo dio svolge tra il primo principio e la materia. In questo modo si mostrerà come i passi del Timeo vengano interpretati da Numenio alla luce della sua metafisica dualista.
A 71-day study was conducted to explore the effect of increasing dietary levels (0, 250, 500, 1000 mg kg feed
; D0, D250, D500 and D1000, respectively) of a blend of microencapsulated organic acids ...(OA, specifically citric and sorbic acid) and nature identical compounds (NIC, specifically thymol and vanillin), on growth, intestinal immune parameters and gut microbiota (GM) of European sea bass juveniles reared under normal and subsequently suboptimal environmental conditions (high temperature, 30.0 ± 0.4 °C and low oxygen, 4.6 ± 0.6 mg L
). OA and NIC did not promote growth, feed utilisation and feed intake at the inclusion tested but induced a significantly upregulation of IL-8, IL-10 and TGFβ. GM analyzed by next-generation sequencing showed that OA and NIC were able to exert prebiotic properties stimulating the development of beneficial bacteria taxa such as Lactobacillus, Leuconostoc, and Bacillus sp. Picrust analyses displayed a significant potential functional reconfiguration of GM promoting a decrease in inflammation-promoting and homeostatic functions at increasing OA and NIC administration. For the first time on this species the exposure to suboptimal rearing conditions was able to modify GM structure reducing LAB and increasing Proteobacteria, findings which were consistent with the inflammatory process observed at mRNA level.
With respect to Parmenides’ thought Melissus was regarded as a dissident thinker already in antiquity. His polemical introduction of the concept of void and the relative idea of infinite Being seemed ...particularly controversial. The aim of the present paper is to examine the origins of the Melissian understanding of void in order to trace its philosophical genesis to the criticism of the Atomist Leucippus. According to the philosopher from Abdera, the Eleatic fundamental principles had to conform to the obviousness of bodies’ motion, which is why the Eleatic not-Being had to be understood as void. Melissus took issue with this view and criticized the idea of the void’s reality by means of a methodical argument. In the course of doing so, the philosopher from Samos distorted the original Parmenidean ontology, which is why his theories were often criticized severely as theoretically weak.
Aquaculture is the fastest-growing food-producing sector, with a global production of 122.6 million tonnes in 2020. Nonetheless, aquatic animal production can be hampered by the occurrence of viral ...diseases. Furthermore, intensive farming conditions and an increasing number of reared fish species have boosted the number of aquatic animals' pathogens that researchers have to deal with, requiring the quick development of new detection and study methods for novel unknown pathogens. In this respect, the molecular tools have significantly contributed to investigating thoroughly the structural constituents of fish viruses and providing efficient detection methods. For instance, next-generation sequencing has been crucial in reassignment to the correct taxonomic family, the sturgeon nucleo-cytoplasmic large DNA viruses, a group of viruses historically known, but mistakenly considered as iridoviruses. Further methods such as in situ hybridisation allowed objectifying the role played by the pathogen in the determinism of disease, as the cyprinid herpesvirus 2, ostreid herpesvirus 1 and betanodaviruses. Often, a combination of molecular techniques is crucial to understanding the viral role, especially when the virus is detected in a new aquatic animal species. With this paper, the authors would critically revise the scientific literature, dealing with the molecular techniques employed hitherto to study the most relevant finfish and shellfish viral pathogens.
The first book of the Aristotelian Physics may be considered as a sort of general introduction to the whole work. In particular, chapters 2 and 3 result very interesting for the foundation of the ...science of nature according to Aristotle; indeed, in these two chapters, the Stagirite criticizes the position of the Eleates Parmenides and Melissus. These two philosophers are considered as those who claim that change does not exist because the existence of the not-being is impossible to suppose. For this reason, since the Eleates deny that motion and change really exist, the refutation of their thesis results essential for the logical and ontological foundation of the Aristotelian science of nature. This paper wants to make light on the argumentations that Aristotle uses against Parmenides and Melissus. Indeed, since the Stagirite makes often use of his philosophical categories (like substance; the categories, the continuum and so on), it seems that his critics sound a little bit anachronistic. In many passages of the text is seems that Aristotle “simply” replace the presupposition of the Eleatic philosophy (i.e. the being is and not-being does not) with the thesis of the evidence of moving and plurality, in general. The aim of this contribution is to reflect on the critics present in Ph. I 2–3 in order to underline the importance of the Eleatic philosophy for the Aristotelian science of nature.
Aquaculture is a constantly growing sector. The intensification of fish production and the movement of aquatic animals could cause the spread of infectious diseases. Remarkably, the diffusion of ...viral agents represents the major bottleneck for finfish production, and viral encephalopathy and retinopathy (VER) is considered the most impacting disease for Mediterranean aquaculture. No effective therapies are available to contrast VER, and vaccination can be applied only in grow-out facilities. Hence, programs to minimize the sanitary risks in farms are paramount to implementing hygienic standards and biosecurity. This study aimed to evaluate the in vitro virucidal activity of a peroxy-acid disinfectant (Virkon
S, DuPont, Sudbury, UK) towards the two NNV strains most widespread in the Mediterranean Sea. Remarkably, two protocols were applied to assess the virucidal activity under different conditions of use: the suspension test and the net test. The latter has been applied to evaluate the efficacy of the biocide on instruments, simulating the in-field application. The obtained results demonstrated the suitability of the tested biocide for NNV inactivation, being effective under some of the tested conditions. However, the presence of organic matter, the concentration of the product, and the application conditions can significantly affect the result of the disinfection procedure.
Marine gastropods represent a major food source for higher trophic levels and an important source of animal protein for humans. Like bivalve molluscs, gastropods can accumulate several types of ...contaminants; however, the bioaccumulation of microorganisms, particularly viruses, has been poorly investigated in these animals. This study focused on gastropods (Tritia mutabilis, Bolinus brandaris and Rapana venosa) collected during the fishing season from 2017 to 2021 in the north-western Adriatic Sea, and on clams (Ruditapes philippinarum) harvested in the same geographical area, in order to evaluate the presence of human and fish viruses in their tissues. A virological investigation was carried out on the digestive gland using molecular methods. The presence of hepatitis A virus was detected in one sample, whereas noroviruses were not present in the investigated specimens. Regarding fish viruses, it was possible to detect the presence of nervous necrosis virus (NNV) in 26.5% of the analyzed gastropods; however, the histological examination did not show any pathological changes in the nervous tissue in both NNV-positive and -negative batches. As a whole, the investigated gastropods showed the ability to bioaccumulate viruses; however, lower contamination by human viruses compared to bivalve molluscs was pointed out, posing a minor concern to human health.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
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