•Role of hypothalamic cold-sensitive ion channels TRPA1 and TRPM8 is unclear.•Temperature homeostasis in thermoneutral conditions and water imbalance was studied.•Water-deprived rats develop ...reactions preventing the decrease of body temperature.•Hyperhydrated rats do not develop such reactions sufficiently.•Development of some reactions correlates with gene expression of TRPA1 and TRPM8.
The role of the hypothalamic cold-sensitive ion channels - transient receptor potential melastatin 8 (TRPM8) and transient receptor potential ankyrin 1 (TRPA1) in homeostatic systems of thermoregulation and water-salt balance – is not clear. The interaction of homeostatic systems of thermoregulation and water-salt balance without additional temperature load did not receive due attention, too. On the models of water-balance disturbance, we tried to elucidate some aspect of these problems.
Body temperature (Tbody), O2 consumption, CO2 excretion, electrical muscle activity (EMA), temperature of tail skin (Ttail), plasma osmolality, as well as gene expression of hypothalamic TRPM8 and TRPA1 have been registered in rats of 3 groups: control; water-deprived (3 days under dry–eating); and hyperhydrated (6 days without dry food, drinking liquid 4 % sucrose). No relationship was observed between plasma osmolality and gene expression of Trpm8 and Trpa1. In water-deprived rats, the constriction of skin vessels, increased fat metabolism by 10 % and increased EMA by 48 % allowed the animals to maintain Tbody unchanged. The hyperhydrated rats did not develop sufficient mechanisms, and their Tbody decreased by 0.8 °C. The development of reactions was correlated with the expression of genes of thermosensitive ion channels in the anterior hypothalamus. Ttail had a direct correlation with the expression of the Trpm8 gene, whereas EMA directly correlated with the expression of the Trpa1 gene in water-deprived group. The obtained data attract attention from the point of view of management and correction of physiological functions by modulating the ion channel gene expression.
Introduction
In accordance with the systematics of modern international clinical guidelines (DSM-V, ICD-11), catatonia is qualified as a transnosological formation, which boundaries expandes by ...including non-psychotic movement disorders (hysterical, affective, negative, etc.). This study presents the psychopathological systematics of movement disorders, based on a new dimensional model of catatonia.
Objectives
60 patients with an established diagnosis of schizophrenia or SSD (F20, F21, F25.01, F25.11, F25.21, F25.22), catatonic disorders in the structure of which persist throughout the course of the disease or determine the clinical picture of phases.
Methods
Clinical, psychometric (BFCRS, SANS, SAPS, HADS), statistic.
Results
Three catatonic syndromes (S.) have been identified. 1. S. of stereotypical catatonia - presented by the mechanism of affiliation with negative symptoms (R between BFCRS Total Score (TS) and Avolition-Apathy SANS – 0,875): tendency to stereotypical activity; general, increasing slowness (SANS avolition-apathy -2,9±0,5; BFCRS TS – 11,1±0,2). 2. S. of parakinetic catatonia - includes paroxysms formes by the mechanism of mental automatism (with the loss of motor acts voluntary effect ) (R BFCRS TS/Persecutory Delusions SAPS– 0,764): irregular polymorphic movement disorders of hyperkinetic and akinetic types, impulsive actions, akinesias (Persecutory Delusions - 2,3±0,4; BFCRS – 19,5±2,3). 3. Affective - catatonic S. - including both the lightest (at the level of recurrent depression) variants of affective-catatonic phases (R BFCRS TS/HADS – 0,732; BFCRS – 5,1±0,4; HADS -15,1±2,4), and more severe affective-catatonic states based on schizoaffective psychoses (R BFCRS TS/SAPS TS– 0,783; BFCRS – 15,3±2,1; SAPS – 3,1±0,2).
Conclusions
Catatonia is not a single dimension, represented by heterogeneous movement disorders, differing both in the mechanism of formation and in the psychopathological structure.
Disclosure
No significant relationships.
•Nanocrystalline cellulose films were obtained by evaporation from aqueous dispersions.•Thermodestruction and surface morphology of the films are studied.•Properties of the films depend on surface ...concentration of sulfate groups.
Properties of films derived from aqueous nanocrystalline cellulose dispersions by water evaporation depend on concentration of sulfate groups. Namely type of thermodestruction and surface morphology change as a function of contents of sulfate groups. Surface roughness increases and water adsorption enhances with increasing sulfate groups content particularly at high relative pressure.
The small subunit ribosomal RNA (SSU rRNA) is widely used phylogenetic marker in broad groups of organisms and its secondary structure increasingly attracts the attention of researchers as ...supplementary tool in sequence alignment and advanced phylogenetic studies. Its comparative analysis provides a great contribution to evolutionary biology, allowing find out how the SSU rRNA secondary structure originated, developed and evolved. Herein, we provide the first data on the putative SSU rRNA secondary structures of the Plagiorchiida species. The structures were found to be quite conserved across broad range of species studied, well compatible with those of others eukaryotic SSU rRNA and possessed some peculiarities: cross-shaped structure of the ES6b, additional shortened ES6c2 helix, and elongated ES6a helix and h39 + ES9 region. The secondary structures of variable regions ES3 and ES7 appeared to be tissue-specific while ES6 and ES9 were specific at a family level allowing considering them as promising markers for digenean systematics. Their uniqueness more depends on the length than on the nucleotide diversity of primary sequences which evolutionary rates well differ. The findings have important implications for understanding rRNA evolution, developing molecular taxonomy and systematics of Plagiorchiida as well as for constructing new anthelmintic drugs.
•The 18S rRNA secondary structure model was first predicted for Trematoda.•The evolutionary rates of variable regions well differ.•Its secondary structures highly depends on the length of primary sequences.•The ES3 and ES7 secondary structures reflect tissue specificity of parasites.•The ES6 and ES9 secondary structures are family specific.
The features of anomalous Siberian wildfires in 2019 and trends of their changes during 2000–2019 are analyzed on the basis of satellite monitoring data. The average values of wildfire areas; total ...volumes of CO, CO
2
, and aerosol PM2.5 emissions; and their density (per area unit) in the Siberian Federal District of Russia and its regions are estimated for a 20-year period, as well as the number, radiative power, and spatial distribution of wildfires on the territory of North Eurasia in 2019. In July 2019, the wildfire area in Irkutsk oblast exceeded the average value for the period of 2001–2019 by more than four times, whereas the related total volumes of emissions of CO, CO
2
, and PM2.5 aerosol in June 2019 were five times higher than their average values for this period. The regional weather-climate features of summer 2019 were revealed using on-land observations and reanalysis, in particular, atmospheric blocking, which is related to the formation of extreme regimes of wildfires and floods in adjacent territories of Siberia.
We theoretically analyze exciton-photon oscillatory dynamics within a homogenous polariton gas in the presence of energy detuning between the cavity and quantum well modes. Whereas pure Rabi ...oscillations consist of the particle exchange between the photon and exciton states in the polariton system without any oscillations of the phases of the two subcondensates, we demonstrate that any nonzero detuning results in oscillations of the relative phase of the photon and exciton macroscopic wave functions. Different initial conditions reveal a variety of behaviors of the relative phase between the two condensates, and a crossover from Rabi-like to Josephson-like oscillations is predicted.
Plastic products are easy and convenient for our everyday use, but their negative impacts on human health and the environment cannot be overlooked. The negative impacts and effects of plastic waste ...are now widely known and have been subject of much recent media coverage, both in Europe and on a global level.
Faced with increasing amounts of plastic waste, the European Union as a whole and many European governments in particular, are currently revising the policy options available to cope with the problem. One of the tools which may be deployed with a view to reducing the pressures posed by plastic waste, is the Extended Producer Responsibility principle. It is considered to be one of the major waste management policy instruments that support the implementation of the European waste hierarchy. Its application may assist in fostering the collection and recycling of waste streams that contain plastic. This paper presents an overview of the problems posed by plastic waste, and outlines their environmental dimensions. It discusses the role of the Extended Producer Responsibility principle and provides some recommendations that may be useful in enhancing extended producer responsibility.
•The production of plastic waste in Europe is increasing.•Many plastic-related policies across Europe are not satisfactory.•The environmental impacts of plastic disposal are significant and deserve immediate action.•Extended Producer Responsibility can assist in reducing the scope of the problem.
The present study is aimed to elucidate the possible involvement of the thermosensitive TRPM2 ion channel in changing of the temperature sensitivity of the hypothalamus after different cold ...exposures-long-term adaptation to cold and short-term cooling. Quantitative RT-PCR was used to study the expression of the gene of thermosensitive TRPM2 ion channel in the hypothalamus in the groups of control (kept for 5 weeks at +20 to +22 °C) and cold-adapted (5 weeks at +4 to +6 °C) rats, as well as in the groups of animals which were subjected to acute cooling (rapid or slow) with subsequent restoration of body temperature to the initial level. It has been shown that after long-term adaptation to cold, the decrease in the
gene expression was observed in the hypothalamus, while a short-term cooling does not affect the expression of the gene of this ion channel. Thus, long-term adaptation to cold results in the decrease in the activity not only of the TRPV3 ion channel gene, as shown earlier, but also of the
gene in the hypothalamus. The overlapping temperature ranges of the functioning of these ion channels and their unidirectional changes during the adaptation of the homoeothermic organism to cold suggest their functional interaction. The decrease in the
gene expression may indicate the participation of this ion channel in adaptive changes in hypothalamic thermosensitivity, but only as a result of long-term cold exposure and not of a short-term cooling. These processes occurring at the genomic level are one of the molecular mechanisms of the adaptive changes.
Ice-binding proteins are crucial for the adaptation of various organisms to low temperatures. Some of these, called antifreeze proteins, are usually thought to inhibit growth and/or recrystallization ...of ice crystals. However, prior to these events, ice must somehow appear in the organism, either coming from outside or forming inside it through the nucleation process. Unlike most other works, our paper is focused on ice nucleation and not on the behavior of the already-nucleated ice, its growth, etc. The nucleation kinetics is studied both theoretically and experimentally. In the theoretical section, special attention is paid to surfaces that bind ice stronger than water and thus can be "ice nucleators", potent or relatively weak; but without them, ice cannot be nucleated in any way in calm water at temperatures above -30 °C. For experimental studies, we used: (i) the ice-binding protein mIBP83, which is a previously constructed mutant of a spruce budworm
antifreeze protein, and (ii) a hyperactive ice-binding antifreeze protein, RmAFP1, from a longhorn beetle
. We have shown that RmAFP1 (but not mIBP83) definitely decreased the ice nucleation temperature of water in test tubes (where ice originates at much higher temperatures than in bulk water and thus the process is affected by some ice-nucleating surfaces) and, most importantly, that both of the studied ice-binding proteins significantly decreased the ice nucleation temperature that had been significantly raised in the presence of potent ice nucleators (CuO powder and ice-nucleating bacteria
). Additional experiments on human cells have shown that mIBP83 is concentrated in some cell regions of the cooled cells. Thus, the ice-binding protein interacts not only with ice, but also with other sites that act or potentially may act as ice nucleators. Such ice-preventing interaction may be the crucial biological task of ice-binding proteins.
ISIAH (inherited stress-induced arterial hypertension) rats are characterized by high blood pressure and decreased
gene expression in the anterior hypothalamus. Thermosensitive ion channel TRPM8 ...plays a critical role in the transduction of moderately cold stimuli that give rise to cool sensations. In normotensive animals, the activation of skin TRPM8 is known to induce changes in gene expression in the hypothalamus and induce alterations of thermoregulatory responses. In this work, in hypertensive rats, we studied the effects of activation of the peripheral TRPM8 by cooling and by application of a 1% menthol suspension on (1) the maintenance of body temperature balance and (2) mRNA expression of thermosensitive TRP ion channels in the hypothalamus. In these hypertensive animals, (1) pharmacological activation of peripheral TRPM8 did not affect the thermoregulatory parameters either under thermoneutral conditions or during cold exposure; (2) the expression of
in the anterior hypothalamus approximately doubled (to the level of normotensive animals) under the influence of (a) slow cooling and (b) at pharmacological activation of the peripheral TRPM8 ion channel. The latter fact seems the quite important because it allows the proposal of a tool for correcting at least some parameters that distinguish a hypertensive state from the normotensive one.
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