Hypoxia-inducible factor 1-α (HIF-1α) plays a critical role in angiogenesis-osteogenesis coupling during bone development and bone regeneration. Previous studies have shown that 17β-estradiol ...activates the HIF-1α signaling pathway and that mice with conditional activation of the HIF-1α signaling pathway in osteoblasts are protected from ovariectomy (OVX)-induced bone loss. In addition, it has been shown that hypoxia facilitates the osteogenic differentiation of mesenchymal stem cells (MSCs) and modulates Wnt/β-catenin signaling. Therefore, we hypothesized that activation of the HIF-1α signaling pathway by hypoxia-mimicking agents would prevent bone loss due to estrogen deficiency. In this study, we confirmed the effect of dimethyloxalylglycine (DMOG), a hypoxia-mimicking agent, on the HIF-1α signaling pathway and investigated the effect of DMOG on MSC osteogenic differentiation and the Wnt/β-catenin signaling pathway. We then investigated the effect of DMOG treatment on OVX-induced bone loss. Female C57BL/6J mice were divided into sham, OVX, OVX+L-DMOG (5 mg/kg/day), and OVX+H-DMOG (20 mg/kg/day) groups. At sacrifice, static and dynamic bone histomorphometry were performed with micro computed tomography (micro-CT) and undecalcified sections, respectively. Bone strength was assessed with the three-point bending test, and femur vessels were reconstructed and analyzed by micro-CT. Serum vascular endothelial growth factor (VEGF), osteocalcin, and C-terminal telopeptides of collagen type(CTX) were measured by ELISA. Tartrate-resistant acid phosphatase staining was used to assess osteoclast formation. Alterations in the HIF-1α and Wnt/β-catenin signaling pathways in the bone were detected by western blot. Our results showed that DMOG activated the HIF-1α signaling pathway, which further activated the Wnt/β-catenin signaling pathway and enhanced MSC osteogenic differentiation. The micro-CT results showed that DMOG treatment improved trabecular bone density and restored the bone microarchitecture and blood vessels in OVX mice. Bone strength was also partly restored in DMOG-treated OVX mice. Dynamic bone histomorphometric analysis of the femur metaphysic revealed that DMOG increased the mineralizing surface, mineral apposition rate, and bone formation rate. The serum levels of VEGF and osteocalcin were higher in DMOG-treated OVX mice. However, there were no significant differences in serum CTX or in the number of tartrate-resistant acid phosphatase-stained cells between DMOG-treated OVX mice and OVX mice. Western blot results showed that DMOG administration partly rescued the decrease in HIF-1α and β-catenin expression following ovariectomy. Collectively, these results indicate that DMOG prevents bone loss due to ovariectomy in C57BL/6J mice by enhancing angiogenesis and osteogenesis, which are associated with activated HIF-1α and Wnt/β-catenin signaling pathways.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Purpose To evaluate bone formation after marsupialization of odontogenic keratocysts (keratocystic odontogenic tumors) of the mandible. Patients and Methods A total of 53 patients with mandibular ...odontogenic keratocysts underwent marsupialization. Clinical and radiographic examinations were done at 1, 3, and 6 months postoperatively. The bone density of the cyst site was measured on the panoramic radiographs using the Digora. The volume of the cyst was measured by injection of saline solution into the cyst cavity. Student t test and Spearman's rank correlation were used for statistical analysis. Results Healing was uneventful in all patients. The diameter of the cysts was 4.1 to 11.0 cm (average 5.4). The panoramic radiographs showed a continuous increase in bone density of the cystic area, with a 22.42% increase at 1 month, 46.07% at 3 months, and 64.69% at 6 months postoperatively compared with the preoperative values. The decrease in cyst volume was 19.05% at 1 month, 55.62% at 3 months, and 79.67% at 6 months postoperatively. The increase in bone density and decrease in cyst volume were more significant in the first 3 months than in second 3 months ( P < .01). The increase in bone density correlated inversely with the decrease in the cyst volume ( P < .01). Conclusion Bone regeneration can occur more rapidly in large mandibular odontogenic keratocysts after marsupialization with drainage by a cyst plug. After 3 months, secondary enucleation of the cyst can be performed.
Three-dimensional (3D) lithiophilic host is one of the most effective ways to regulate the Li dendrites and volume change in working Li metal anode. The state-of-the-art 3D lithiophilic hosts are ...facing one main challenge in that the lithiophilic layer would melt or fall off in high-temperature environment when using the thermal infusion method. Herein, a 3D porous CuZn alloy host containing anchored lithiophilic Zn sites is employed to prestore Li using the thermal infusion strategy, and a 3D composite Li is thus fabricated. Benefiting from the lithiophilic Zn sites with a strong adsorption capacity with Li, which is based on the analyses of the nucleation overpotential, binding energy calculation, and the operando optical observation of Li plating/stripping behaviors, facile uniform Li nucleation and dendrite-free Li deposition could be achieved in the interior of the 3D porous CuZn alloy host and the 3D composite Li shows remarkable enhancement in electrochemical performance.
Patients with critical illness due to infection with the 2019 coronavirus disease (COVID-19) show rapid disease progression to acute respiratory failure. The study aimed to screen the most useful ...predictive factor for critical illness caused by COVID-19.
The study prospectively involved 61 patients with COVID-19 infection as a derivation cohort, and 54 patients as a validation cohort. The predictive factor for critical illness was selected using LASSO regression analysis. A nomogram based on non-specific laboratory indicators was built to predict the probability of critical illness.
The neutrophil-to-lymphocyte ratio (NLR) was identified as an independent risk factor for critical illness in patients with COVID-19 infection. The NLR had an area under receiver operating characteristic of 0.849 (95% confidence interval CI, 0.707 to 0.991) in the derivation cohort and 0.867 (95% CI 0.747 to 0.944) in the validation cohort, the calibration curves fitted well, and the decision and clinical impact curves showed that the NLR had high standardized net benefit. In addition, the incidence of critical illness was 9.1% (1/11) for patients aged ≥ 50 and having an NLR < 3.13, and 50% (7/14) patients with age ≥ 50 and NLR ≥ 3.13 were predicted to develop critical illness. Based on the risk stratification of NLR according to age, this study has developed a COVID-19 pneumonia management process.
We found that NLR is a predictive factor for early-stage prediction of patients infected with COVID-19 who are likely to develop critical illness. Patients aged ≥ 50 and having an NLR ≥ 3.13 are predicted to develop critical illness, and they should thus have rapid access to an intensive care unit if necessary.
Topological transitions between considerably different phases typically require harsh conditions to collectively break chemical bonds and overcome the stress caused to the original structure by ...altering its correlated bond environment. In this work we present a case system that can achieve rapid rearrangement of the whole lattice of a metal-organic framework through a domino alteration of the bond connectivity under mild conditions. The system transforms from a disordered metal-organic framework with low porosity to a highly porous and crystalline isomer within 40 s following activation (solvent exchange and desolvation), resulting in a substantial increase in surface area from 725 to 2,749 m
g
. Spectroscopic measurements show that this counter-intuitive lattice rearrangement involves a metastable intermediate that results from solvent removal on coordinatively unsaturated metal sites. This disordered-crystalline switch between two topological distinct metal-organic frameworks is shown to be reversible over four cycles through activation and reimmersion in polar solvents.
Traditional object detectors employ the dense paradigm of scanning over locations and scales in an image. The recent query-based object detectors break this convention by decoding image features with ...a set of learnable queries. However, this paradigm still suffers from slow convergence, limited performance, and design complexity of extra networks between backbone and decoder. In this paper, we find that the key to these issues is the adaptability of decoders for casting queries to varying objects. Accordingly, we propose a fast-converging query-based detector, named AdaMixer, by improving the adaptability of query-based decoding processes in two aspects. First, each query adaptively samples features over space and scales based on estimated offsets, which allows AdaMixer to efficiently attend to the coherent regions of objects. Then, we dynamically decode these sampled features with an adaptive MLP-Mixer under the guidance of each query. Thanks to these two critical designs, AdaMixer enjoys architectural simplicity without requiring dense attentional encoders or explicit pyramid networks. On the challenging MS COCO benchmark, AdaMixer with ResNet-50 as the backbone, with 12 training epochs, reaches up to 45.0 AP on the validation set along with 27.9 APs in detecting small objects. With the longer training scheme, AdaMixer with ResNeXt-101-DCN and Swin-S reaches 49.5 and 51.3 AP. Our work sheds light on a simple, accurate, and fast converging architecture for query-based object detectors. The code is made available at https://github.com/MCG-NJU/AdaMixer.
► High-efficient method for extraction of multiple bioactive substances from green tea. ► Sequential selective resin adsorptions to separate bioactive compounds. ► More than 95% extraction efficiency ...with 4-fold of solvent. ► High concentration and low impurities of extraction solution. ► Minimum solvent used, minimum solvent concentration and minimum equipment costs.
A highly efficient column-chromatographic extraction (CCE) followed by sequential adsorption to extract and separate bioactive compounds from green tea was developed. Tea powder was loaded into columns with 4-fold solvents and eluted through a cyclic CCE. High-quality tea extracts with greater than 90% extraction efficiencies of polyphenols, epigallocatechin-3-gallate, caffeine, theanine and polysaccharides were obtained with 4-fold water circulated five times among different columns at 70°C. Similar results, except for low polysaccharide extraction (35.5%), were obtained with 4-fold 30% ethanol circulated three times at room temperature. The highly concentrated water extraction was directly passed through columns of polyamide, DM130 macroporous and 732 ion exchange resins, resulting in high-purity polyphenols (99%), caffeine (98%) and theanine (98%) after simple purification of the eluates from each column. This method uses simple equipment, minimum solvents and can be used for both quantitative analysis and continuous preparation of high-quality tea extracts and bioactive compounds.
Direct light‐to‐work conversion enables manipulating remote devices in a contactless, controllable, and continuous manner. Although some pioneering works have already proven the feasibility of ...controlling devices through light‐irradiation‐induced surface tension gradients, challenges remain, including the flexible integration of efficient photothermal materials, multifunctional structure design, and fluidic drag reduction. This paper reports a facile one‐step method for preparing light‐driven floating devices with functional surfaces for both light absorption and drag reduction. The direct laser writing technique is employed for both arbitrary patterning and surface modification. By integrating the functional layer at the desired position or by designing asymmetric structures, three typical light‐driven floating devices with fast linear or rotational motions are demonstrated. Furthermore, these devices can be driven by a variety of light sources including sunlight, a filament lamp, or laser beams. The approach provides a simple, green, and cost‐effective strategy for building functional floating devices and smart light‐driven actuators.
A facile fabrication of superhydrophobic polydimethylsiloxane (PDMS) elastomers structures that permit controllable manipulation via Marangoni effectthat permit controllable manipulation via Marangoni effect is reported here. Direct laser writing technology is employed to apply a light absorbing and superhydrophobic layer on the PDMS surface. By integrating the functional layer at the desired position or by designing asymmetric structures, typical light‐driven devices with fast linear or rotational motions are demonstrated.
Background and Aim
Although several randomized controlled trials (RCTs) have reported that supplemental simethicone (SIM) can improve bowel preparation based on polyethylene glycol, there is no ...consensus as to whether SIM can ultimately increase the adenoma detection rate (ADR) during colonoscopy. A meta‐analysis was performed to assess the effect of SIM on ADR during colonoscopy.
Methods
Databases including PubMed, EMBASE, and the Cochrane Library were searched to find relevant RCTs. RCTs evaluating the effect of pre‐procedure SIM on the ADR during colonoscopy were finally included, and fixed effect models were applied.
Results
Six trials involving 1855 patients were finally included. The present meta‐analysis suggested that the ADR during colonoscopy was significantly increased by supplemental SIM (27.9% vs 23.3%, P = 0.02), with a relative risk of 1.20 (95% confidence interval 1.03–1.39). Subgroup analysis suggested that supplemental SIM may be more useful to improve ADR during colonoscopy in endoscopic centers with low baseline ADR.
Conclusions
Supplemental SIM for bowel preparation based on polyethylene glycol is useful to improve the ADR during colonoscopy.
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