Accumulating evidence has established that long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) is a tumor regulator in many cancers. Here, we aimed to investigate the possible ...function of lncRNA PVT1 in esophageal carcinoma (EC) via targeting of microRNA‐145 (miR‐145). Initially, microarray‐based gene expression profiling of EC was employed to identify differentially expressed genes. Moreover, the expression of lncRNA PVT1 was examined and the cell line presenting with the highest level of lncRNA PVT1 expression was selected for subsequent experiments. We then proceeded to examine interaction among lncRNA PVT1, FSCN1, and miR‐145. The effect of lncRNA PVT1 on viability, migration, invasion, apoptosis, and tumorigenesis of transfected cells was examined with gain‐of‐function and loss‐of‐function experiments. We observed that lncRNA PVT1 was robustly induced in EC. lncRNA PVT1 could bind to miR‐145 and regulate its expression, and FSCN1 is a target gene of miR‐145. Overexpression of miR‐145 or silencing of lncRNA PVT1 was revealed to suppress cell viability, migration, and invasion abilities, while also stimulating cell apoptosis. Furthermore, our in vivo results showed that overexpression of miR‐145 or silencing of lncRNA PVT1 resulted in decreased tumor growth in nude mice. In conclusion, our research reveals that down‐regulation of lncRNA PVT1 could potentially promote expression of miR‐145 to repress cell migration and invasion, and promote cell apoptosis through the inhibition of FSCN1. This highlights the potential of lncRNA PVT1 as a therapeutic target for EC treatment.
lncRNA PVT1 can specifically compete with miR‐145 to inhibit its expression, thereby increasing FSCN1 expression. This in turn promotes proliferation, invasion, and migration of EC cells by up‐regulating the expression of MAT1, CD147, and VEGFR2. Moreover, down‐regulation of lncRNA PVT1 can up‐regulate expression of miR‐145 to inhibit the expression of FSCN1.
Soybean yield has traditionally been increased through high planting density, but investigating plant height and petiole traits to select for compact architecture, lodging resistance, and high yield ...varieties is an underexplored option for further improving yield. We compared the relationships between yield-related traits, lodging resistance, and petiole-associated phenotypes in the short petiole germplasm M657 with three control accessions during 2017–2018 in four locations in the Huang–Huai region, China. The results showed that M657 exhibited stable and high tolerance to high planting density and resistance to lodging, especially at the highest density (8×105 plants ha−1). The regression analysis indicated that a shorter petiole length was significantly associated with increased lodging resistance. The yield analysis showed that M657 achieved higher yields under higher densities, especially in the northern part of the Huang–Huai region. Among the varieties, there were markedly different responses to intra- and inter-row spacing designs with respect to both lodging and yield that were related to location and density. Lodging was positively correlated with planting density, plant height, petiole length, and number of effective branches, but negatively correlated with stem diameter, seed number per plant, and seed weight per plant. The yield of soybean was increased by appropriately increasing the planting density on the basis of the current soybean varieties in the Huang–Huai region. This study provides a valuable new germplasm resource for the introgression of compact architecture traits that are amenable to providing a high yield in high density planting systems, and it establishes a high-yield model of soybean in the Huang–Huai region.
Nested Association Mapping（NAM） population is widely applied in genetic study and breeding practice in many crops.A NAM panel was constructed by crossing of 35 parental lines with the common maternal ...lines（Zhongdou 41） based on previous evaluation of soybean germplasm. Principle component analysis and clustering analysis showed that clear genetic structure was observed between subpanel of RIL populations. Genetic analysis was performed on flower color and seed coat color in NAM subpanel with significant difference between paternal and maternal parents, and we found that q FC13-1 was significantly associated with flower color, which coincided with the W1 locus. Twelve loci identified were significantly correlated with seed coat color, among which nine loci were co-located by more than three methods, and the other three loci were colocated by two methods, including four reported loci and eight novel loci. In conclusion, NAM population was suitable for genetic analysis of soybean,which provided material basis fo
Leaf is the main organ of soybean photosynthetic carbon assimilation, and its color is related to light energy capture and transformation efficiency, and is also closely related to soybean yield. It ...is of great significance to analyze soybean yield by assimilation pathway. The yellow-green leaf is a mutation type that is different from ordinary green leaves of soybean, and is an important genetic material for studying soybean leaf color-related genes. In this study, a yellow-green leaf mutant ygl2 (yel-low- green leaf 2), this mutant is naturally mutated from soybean line GL11, and its yellow-green leaf phenotype can be stably inherited. Compared with the green leaf wild-type GL11, the chlorophyll content in the leaves of the mutant ygl2 is extremely significantly reduced, the plant height, 100 There were significant differences in grain weight and protein content. Segregating populations were constructed using GL11 and ygl2. Genetic analysis showed that the yellow-green leaf phenotype of ygl2 was controlled
Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) is correlated to various malignant tumors. Consequently, we explored effects of lncRNA PVT1 on esophageal carcinoma (EC) ...targeting microRNA-145 (miR-145). EC tissues, adjacent normal tissues, and EC-related cell lines were collected and cultured. Expression of lncRNA PVT1, miR-145, fascin-1 (FSCN1), and related genes with intervening expression of PVT1 and miR-145 was determined. Bioinformatic website, dual-luciferase reporter assay, and RNA immunoprecipitation (RIP) were carried to verify target relationship among lncRNA PVT1, FSCN1, and miR-145. Scratch test, Transwell assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and flow cytometry were performed for detection of migration, invasion, viability, and apoptosis of transfected cells, respectively. Finally, tumor formation in nude mice was measured. After database analysis, lncRNA PVT1, miR-145, and FSCN1 were selected for study. lncRNA PVT1 and FSCN1 can bind to miR-145. After overexpressing miR-145 or inhibiting lncRNA PVT1, EC cell viability, migration, and invasion were inhibited, while volume and weight of tumor formation in nude mice decreased. Expression of lncRNA PVT1, FSCN1, Bcl-2, CD147, VEGFR2, and MTA1 decreased and expression of miR-145 and Bax increased. Silencing lncRNA PVT1 can upregulate miR-145, which is a tumor suppressor in EC via knockdown of FSCN1. Thus, we might provide a potential theoretical basis for EC treatment.
Accumulating evidence has established that long noncoding
RNA
(lnc
RNA
) plasmacytoma variant translocation 1 (
PVT
1) is a tumor regulator in many cancers. Here, we aimed to investigate the possible ...function of lnc
RNA PVT
1 in esophageal carcinoma (
EC
) via targeting of micro
RNA
‐145 (miR‐145). Initially, microarray‐based gene expression profiling of
EC
was employed to identify differentially expressed genes. Moreover, the expression of lnc
RNA PVT
1 was examined and the cell line presenting with the highest level of lnc
RNA PVT
1 expression was selected for subsequent experiments. We then proceeded to examine interaction among lnc
RNA PVT
1,
FSCN
1, and miR‐145. The effect of lnc
RNA PVT
1 on viability, migration, invasion, apoptosis, and tumorigenesis of transfected cells was examined with gain‐of‐function and loss‐of‐function experiments. We observed that lnc
RNA PVT
1 was robustly induced in
EC
. lnc
RNA PVT
1 could bind to miR‐145 and regulate its expression, and
FSCN
1 is a target gene of miR‐145. Overexpression of miR‐145 or silencing of lnc
RNA PVT
1 was revealed to suppress cell viability, migration, and invasion abilities, while also stimulating cell apoptosis. Furthermore, our
in vivo
results showed that overexpression of miR‐145 or silencing of lnc
RNA PVT
1 resulted in decreased tumor growth in nude mice. In conclusion, our research reveals that down‐regulation of lnc
RNA PVT
1 could potentially promote expression of miR‐145 to repress cell migration and invasion, and promote cell apoptosis through the inhibition of
FSCN
1. This highlights the potential of lnc
RNA PVT
1 as a therapeutic target for
EC
treatment.
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