In order to obtain the influence law of scanning strategy on the quality, accuracy, microstructure and performance of parts by Laser Additive Manufacturing (LAM), three scanning strategies, namely ...long-edge, short-edge and cross-layer, were used for additive manufacturing of Inconel 625 parts. Optical Microscope (OM) experiments indicate that the microstructures of samples with different scanning strategies are different. The results of Scanning Electron Microscopy (SEM) suggest that the size and distribution of Laves phase of samples with the different scanning strategies are distinctive, and Energy Dispersive Spectroscopy (EDS) tests indicate that the degree of composition segregation varies according to the scanning strategy. The results of X-ray Diffraction (XRD) show that the main phase of the samples with different scanning strategies is γ-Ni solid solution. The diffraction peak positions are basically the same, but the peak intensities are diverse, indicating that the scanning strategy has a significant impact on the orientation of grains and the intensity of texture. The mechanical properties tests prove that the cross-layer scanning strategy synthesizes the advantages of the other two scanning strategies. The microhardness test shows that the hardness of samples with different scanning strategies is determined by the size, distribution and morphology of Laves phase, as well as the size of grains. As a result, it can be proved that the scanning strategy has a great influence on the quality, accuracy, microstructure and properties of the as-formed samples. The cross-layer orthogonal scanning strategy achieves the best processing results by combining the advantages of long-edge and short-edge scanning strategies.
Mori fructus aqueous extracts (MFAEs) have been used as a traditional Chinese medicine for thousands of years with the function of strengthening the liver and tonifying the kidney. However, its inner ...mechanism to alleviative renal injury is unclear. To investigate the attenuation of MFAEs on nephrotoxicity and uncover its potential molecular mechanism, we established a nephrotoxicity model induced by carbon tetrachloride (CCl4). The mice were randomly divided into control group, CCl4 model group (10% CCl4), CCl4 + low and high MFAEs groups (10% CCl4 + 100 mg/kg and 200 mg/kg MFAEs). We found that MFAEs decreased the kidney index of mice, restored the pathological changes of renal structure induced by CCl4, reduced cystatin C, neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule 1 (Kim-1) blood urea nitrogen and creatinine contents in serum, promoted the nuclear transportation of Nrf2 (nuclear factor erythroid derived 2 like 2), elevated the expression of HO-1 (heme oxygenase 1), GPX4 (glutathione peroxidase 4), SLC7A11 (solute carrier family 7 member 11), ZO-1 (zonula occludens-1) and Occludin, suppressed the expression of Keap1 (kelch-like ECH-associated protein 1), HMGB1 (High Mobility Group Protein 1), ACSL4 (acyl-CoA synthetase long chain family member 4) and TXNIP (thioredoxin interacting protein), upregulated the flora of Akkermansia, Anaerotruncus, Clostridium_sensu_stricto, Ihubacter, Alcaligenes, Dysosmobacter, and downregulated the flora of Clostridium_XlVa, Helicobacter, Paramuribaculum. Overlapped with Disbiome database, Clostridium_XlVa, Akkermansia and Anaerotruncus may be the potential genera treated with renal injury. It indicated that MFAEs could ameliorate kidney injury caused by CCl4 via Nrf2 signaling.
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•Mori Fructus aqueous extracts (MFAEs) relieved chronic renal injury through ferroptosis, fibrosis and intestinal flora.•Nrf2 signaling was activated by MFAEs to decrease ferroptosis in renal.•MFAEs regulated the abundance of intestinal flora and affected the progression of renal injury.
This study investigates the interplay between physical literacy, resilience, and burnout among researchers who experienced strict home quarantine during the COVID-19 pandemic in China, with a ...particular focus on the mediating role of resilience. Employing a two-stage sampling procedure, a total of 363 researchers from diverse disciplines, notably social science and natural science, were enlisted and administered a series of validated scales, including the Perceived Physical Literacy Scale (PPL), the 10-item Connor–Davidson Resilience Scale (CD-RISC-10), and the Chinese version of the Oldenburg Burnout Inventory (OLBL), via an anonymous online platform. The findings revealed substantial differences in physical literacy, resilience, and burnout across disciplines. Resilience partially mediated the relationship between physical literacy and burnout. Upon a closer examination of the sub-dimensions, resilience was found to fully mediate between factors of motivation, exhaustion, and disengagement. Moreover, one aspect of physical literacy—interaction with the environment—exhibited weaker correlations with both resilience and burnout compared to other dimensions of physical literacy. Overall, the study confirms the significant correlation between physical literacy and psychological parameters, establishing that elevated levels of both physical literacy and resilience serve as key factors in mitigating burnout during the pandemic.
Introduction. Recent years have seen a pressing need to dispose of municipal solid waste due to rapid urbanization. The municipal solid waste incineration fly ash (MSWIFA) produced from solid waste ...incineration power plant exhibits pozzolanic properties and poses concern of toxicity leaching when used directly as building materials. This paper presents an alkali-activation method to produce sustainable alkali-activated MSWIFA materials (AAFMs) with various MSWIFA dosages and investigate the corresponding fabrication and performance. Materials and Methods. Composited alkali activators activate the MSWIFA with constant alkalinity of 5% and the molar ratio of Si/Na = 0.86. The resulting geopolymers' bulk densities, mineral composites, morphology, and compression strength are thoroughly examined. Results and discussions. Results show that the use of MSWIFA may lead to more loose structures because the bubbles are generated from metallic aluminum and alkali activators. Additionally, the production of multiple crystals also accounts for increasing porosity. The generated multi-crystals such as Sylvite, Halite, Hydrocalumite, Calcium Hydroxide, and Ettringite are further detected from the morphology and mineral analysis. Furthermore, compression tests and toxicity characteristic leaching procedures (TCLP) are conducted to investigate the mechanical performance and heavy metals solidification performance of AAFMs, with an optimal compression strength of 19.99MPa at 28 days for AAFM-10 while toxicity leaching is subject to regularity limits. Conclusions. This study shows that great potential of using the alkali-activation method to recycle hazardous municipal solid fly ash into construction materials with both ecological safety and high performance.
Pingwei San (PWS) has been used for more than a thousand years as a traditional Chinese medicine prescription for treating spleen-deficiency diarrhea (SDD). Nevertheless, the exact mechanism by which ...it exerts its antidiarrheal effects remains unclear. The objective of this investigation was to explore the antidiarrheal efficacy of PWS and its mechanism of action in SDD induced by Rhubarb. To this end, UHPLC-MS/MS was used to identify the chemical composition of PWS, while the body weight, fecal moisture content, and colon pathological alterations were used to evaluate the effects of PWS on the Rhubarb-induced rat model of SDD. Additionally, quantitative polymerase chain reaction (qPCR) and immunohistochemistry were employed to assess the expression of inflammatory factors, aquaporins (AQPs), and tight junction markers in the colon tissues. Furthermore, 16S rRNA was utilized to determine the impact of PWS on the intestinal flora of SDD rats. The findings revealed that PWS increased body weight, reduced fecal water content, and decreased inflammatory cell infiltration in the colon. It also promoted the expression of AQPs and tight junction markers and prevented the loss of colonic cup cells in SDD rats. In addition, PWS significantly increased the abundance of
,
, and
, while decreasing the abundance of
and
in the feces of SDD rats. The LEfSe analysis revealed that
,
, and
were relatively enriched in the PWS group. Overall, the findings of this study indicate that PWS exerted a therapeutic effect on Rhubarb-induced SDD in rats by both protecting the intestinal barrier and modulating the imbalanced intestinal microbiota.
By using inter-class and intra-class K-Nearest Neighbors (KNNs), weighted twin support vector machine (WLTSVM) mines as much potential similarity information in samples as possible to improve the ...common short-coming of nonparallel hyperplane classifiers. Multi-view learning (MVL) has a lot of potential due to the multi-modal datasets that are becoming available. In this paper, we propose a new multi-view learning with privileged weighted twin support vector machine (MPWTSVM). It not only inherits the advantages of WLTSVM but also has its characteristics. Firstly, it enhances generalization ability by mining intra-class information from the same perspective. Secondly, it reduces the redundant constraints with the help of inter-class information, thus improving the running speed. Most importantly, it can follow both the consensus and the complementary principles simultaneously. The consensus principle is realized by minimizing the coupling items of different views in the original objective function. The complementary principle is achieved by establishing privileged information paradigms and MVL. Compared with state-of-the-art MVL methods: SVM-2K, MVTSVM, MCPK, PSVM-2V, MVRDTSVM and MVTHSVM-2C, our model has better accuracy and classification efficiency. Experimental results on numerous datasets prove the effectiveness of the proposed algorithm.
•A new multi-view classification method (MPWTSVM) is proposed.•MPWTSVM follows both the consensus and the complementary principle.•MPWTSVM enhances generalization ability by mining intra-class information.•MPWTSVM reduces the redundancy constraints by inter-class information.•MPWTSVM establishes privileged information paradigms.
Commonly used reporters rely on a single property, such as the fluorescence of GFP and visible color of anthocyanins, therefore these reporters hardly handle the complicated condition in practice. ...Betaxanthins are a group of plant natural products derived from the amino acid tyrosine. Its visible yellow-orange color and green fluorescence under blue light make it a promising new reporter. Only two enzymatic reactions are required to convert tyrosine into betaxanthins. Here, we synthesized an open reading frame named Bx that contained all the betaxanthins biosynthetic genes and demonstrated its use as a powerful and efficient reporter in tobacco, carrot, and tomato.
Liver fibrosis and hepatocellular carcinogenesis secondary to liver fibrosis are serious liver diseases with no effective treatments. Mori fructus aqueous extracts (MFAEs) have served as successful ...treatments for many types of liver injury including fibrosis although the molecular mechanisms are unknown at present.
To investigate the effect of MFAEs in alleviating acute and chronic liver injury and tried to decipher the underlying mechanism.
Mice were divided into 5 groups (n = 8) for acute (groups: control, 0.3% CCl
, bifendate (BD), 100 and 200 mg/kg MFAEs, 7 d) and chronic (groups: control, 10% CCl
, BD, 100 and 200 mg/kg MFAEs, 4 weeks) liver injury study. Each mouse was injected intraperitoneally with 10 µL/g corn oil containing CCl
expect the control group. HepG2 cells were used in vitro study. Eighteen communal components were identified by UPLC-LTQ-Orbitrap-MS. We utilized a mouse model for acute and chronic liver injury using CCl
and MFAEs administration effectively blocked fibrosis and significantly inhibited inflammation in the liver. MFAEs activated the nuclear factor erythroid derived 2 like 2/heme oxygenase 1 (Nrf2/HO-1) pathway and promoted the synthesis of the antioxidants glutathione (GSH), superoxidedismutase (SOD) and glutathione peroxidase (GSH-Px) that resulted in reduced levels of CCl
-induced oxidative stress molecules including reactive oxygen species. These extracts administered to mice also inhibited ferroptosis in the liver by regulating the expression of Acyl-CoA synthetase long chain family member 4 (ACSL4), solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4), thus reducing the occurrence of liver fibrosis. Both in vivo and in vitro tests indicated that the mechanism of MFAEs protection against liver fibrosis was linked to activation of Nrf2 signaling. These effects were blocked in vitro by the addition of a specific Nrf2 inhibitor.
MFAEs inhibited oxidative stress, ferroptosis and inflammation of the liver by activating Nrf2 signal pathway and provided a significant protective effect against CCl
-induced liver fibrosis.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
In the carbon capture and storage (CCS) infrastructure, the risk of a high-pressure buried pipeline rupture possibly leads to catastrophic accidents due to the release of tremendous amounts of carbon ...dioxide (CO
2
). Therefore, a comprehensive understanding of the effects of CO
2
dispersion pattern after release from CCS facilities is essential to allow the appropriate safety precautions to be taken. Due to variations in topography above the pipeline, the pattern of CO
2
dispersion tends to be affected by the real terrain features, such as trees and hills. However, in most previous studies, the dynamic impact of trees on the wind field was often approximated to linear treatment or even ignored. In this article, a computational fluid dynamics (CFD) model was proposed to predict CO
2
dispersion over shrubbery areas. The shrubs were regarded as a kind of porous media, and the model was validated against the results from experiment. It was found that shrubbery affected the flow field near the ground, enhancing the lateral dispersion of CO
2
. Compared with that of the shrub-free terrain, the coverage area of the three shrub terrains at 60 s increased by 8.1 times, 6.7 times, and 9.1 times, respectively. The influence of shrub height and porosity on CO
2
dispersion is nonlinear. This research provides reliable data for the risk assessment of CCS.
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