Polylactic acid/Hydroxyapatite (PLA/HA) composite was widely studied and applied in the field of biomaterials for its good processability, bioactivity, and mechanical properties. In addition to ...traditional preparation methods, additive manufacturing has also been adopted to prepare PLA/HA composites with customized geometries. This work combined the comprehensive optimized PLLA (L-polylactic acid)/nano-HA (nHA) composite with the low-cost and stable Fused deposition modeling (FDM) technology to successfully prepare PLLA/nHA porous bone repair scaffolds. The results showed that PLLA/nHA composite ink satisfied the smoothness of printing, and the accuracy also met the requirements of personalized bone repair application. The high loaded nHA scaffold had suitable compressive strength was significantly higher than those of pure HA ceramic scaffold and cancellous bone. Besides, in vitro bone-like apatite formation on the surface in the degradation process and in vivo evaluations further verified its good osteogenic property. Compared with other complex and cutting-edge 3D printing technologies, this study provides a low-cost, stable, simple and fast way to realize personalized printing of bone repair scaffolds, which is undoubtedly conductive to the improvement and rapid deployment of personalized biomaterials in clinical applications.
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•Comprehensive optimized PLLA/nano-hydroxyapatite composite was made successfully for bone repair scaffold printing.•Bone-like apatite can form on the scaffold surface in vitro degradation experiments just in PBS, indicating the high bioactivity of scaffold.•The high loaded nano- hydroxyapatite scaffold has suitable compressive strength and good osteogenic property.•This study provides a low-cost, stable, simple and fast way to realize personalized printing of bone repair scaffolds.
Adhesive hydrogels have gained popularity in biomedical applications, however, traditional adhesive hydrogels often exhibit short-term adhesiveness, poor mechanical properties and lack of ...antibacterial ability. Here, a plant-inspired adhesive hydrogel has been developed based on Ag-Lignin nanoparticles (NPs)triggered dynamic redox catechol chemistry. Ag-Lignin NPs construct the dynamic catechol redox system, which creates long-lasting reductive-oxidative environment inner hydrogel networks. This redox system, generating catechol groups continuously, endows the hydrogel with long-term and repeatable adhesiveness. Furthermore, Ag-Lignin NPs generate free radicals and trigger self-gelation of the hydrogel under ambient environment. This hydrogel presents high toughness for the existence of covalent and non-covalent interaction in the hydrogel networks. The hydrogel also possesses good cell affinity and high antibacterial activity due to the catechol groups and bactericidal ability of Ag-Lignin NPs. This study proposes a strategy to design tough and adhesive hydrogels based on dynamic plant catechol chemistry.
2D conductive nanosheets are central to electronic applications because of their large surface areas and excellent electronic properties. However, tuning the multifunctions and hydrophilicity of ...conductive nanosheets are still challenging. Herein, a green strategy is developed for fabricating conductive, redox‐active, water‐soluble nanosheets via the self‐assembly of poly(3,4‐ethylenedioxythiophene) (PEDOT) on the polydopamine‐reduced and sulfonated graphene oxide (PSGO) template. The conductivity and hydrophilicity of nanosheets are highly improved by PSGO. The nanosheets are redox active due to the abundant catechol groups and can be used as versatile nanofillers in developing conductive and adhesive hydrogels. The nanosheets create a mussel‐inspired redox environment inside the hydrogel networks and endow the hydrogel with long‐term and repeatable adhesiveness. This hydrogel is biocompatible and can be implanted for biosignals detection in vivo. This mussel‐inspired strategy for assembling 2D nanosheets can be adapted for producing diverse multifunctional nanomaterials, with various potential applications in bioelectronics.
Inspired by redox reactions in nature, a green and cost‐effective strategy is developed for designing hydrophilic, conductive, and redox‐active sandwich‐like 2D nanosheets via the self‐assembly of poly(3,4‐ethylenedioxythiophene) (PEDOT) on a functional graphene oxide (PSGO) template. The 2D nanosheet can be used as a versatile nanofiller in the development of a conductive and adhesive hydrogel for bioelectronic applications.
Conductive hydrogels (CHs) have gained significant attention for their wide applications in biomedical engineering owing to their structural similarity to soft tissues. However, designing CHs that ...combine biocompatibility with good mechanical and electrical properties is still challenging. Herein, we report a new strategy for the fabrication of tough CHs with excellent conductivity, superior mechanical properties, and good biocompatibility by using chitosan framework as molecular templates for controlling conducting polypyrrole (PPy) nanorods in situ formation inside the hydrogel networks. First, polyacrylamide/chitosan (CS) interpenetrating polymer network hydrogel was synthesized by UV photopolymerization; second, hydrophobic and conductive pyrrole monomers were absorbed and fixed on CS molecular templates and then polymerized with FeCl3 in situ inner hydrophilic hydrogel network. This strategy ensured that the hydrophobic PPy nanorods were uniformly distributed and integrated with the hydrophilic polymer phase to form highly interconnected conductive path in the hydrogel, endowing the hydrogel with high conductivity (0.3 S/m). The CHs exhibited remarkable mechanical properties after the chelation of CS by Fe3+ and the formation of composites with the PPy nanorods (fracture energy 12 000 J m–2 and compression modulus 136.3 MPa). The use of a biopolymer molecular template to induce the formation of PPy nanostructures is an efficient strategy to achieve conductive multifunctional hydrogels.
Accumulating evidence indicates that long non-coding RNAs (lncRNAs) play a crucial role in tumorigenesis. Here, we report a novel lncRNA, RP11-436H11.5, that regulates renal cell carcinoma (RCC) cell ...proliferation and invasion by sponging miR-335-5p.
Expression of lncRNA RP11-436H11.5 was determined by a qRT-PCR assay in RCC tissues. RCC cell proliferation and invasion were measured by a cell proliferation assay and a transwell invasion assay. Expression of BCL-W was detected by a western blot assay. Interactions between lncRNA RP11-436H11.5 and miR-335-5p were measured by a luciferase reporter assay and a RNA-pull down assay. In vivo experiments were used to detect tumor formation.
In this study, the qRT-PCR results illustrated that lncRNA RP11-436H11.5 was more highly expressed in RCC tissues than in adjacent normal renal tissues. The results of survival analysis indicated that patients in the high lncRNA RP11-436H11.5 group presented significantly worse outcomes compared with those in the low lncRNA RP11-436H11.5 group. Downregulation of lncRNA RP11-436H11.5 suppressed RCC cell proliferation and invasion in vitro and in vivo. Luciferase reporter assay results demonstrated that lncRNA RP11-436H11.5 enhanced BCL-W expression by regulating miR-335-5p expression. LncRNA RP11-436H11.5 could function as a miR-335-5p decoy to derepress expression of BCL-W.
LncRNA RP11-436H11.5 could function as a competing endogenous RNA to promote RCC cell proliferation and invasion, which might serve as a therapeutic application to suppress RCC progression.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Nowadays, the state‐of‐the‐art electrocatalysts for hydrogen evolution reaction (HER) are platinum group metals. Nonetheless, Pt‐based catalysts show decreased HER activity in alkaline media compared ...with that in acidic media due to the sluggish dissociation process of H2O on the surface of Pt. With a cost 1/25 that of Pt, Ru demonstrates a favorable dissociation kinetics of absorbed H2O. Herein, crystalline Ru0.33Se nanoparticles are decorated onto TiO2 nanotube arrays (TNAs) to fabricate Ru0.33Se @ TNA hybrid for HER. Owing to the large‐specific surface area, Ru0.33Se nanoparticles are freely distributed and the particle aggregation is eliminated, providing more active sites. The contracted electron transport pathway rendered by TiO2 nanotubes and the synergistic effect at the interface significantly improve the charge transfer efficiency in the hybrid catalyst. Compared with Ru0.33Se nanoparticles deposited directly on the Ti foil (Ru0.33Se/Ti) or carbon cloth (Ru0.33Se/CC), Ru0.33Se @ TNA shows an enhanced catalytic activity with an overpotential of 57 mV to afford a current density of 10 mA cm−2, a Tafel slope of 50.0 mV dec−1. Furthermore, the hybrid catalyst also exhibits an outstanding catalytic stability. The strategy here opens up a new synthetic avenue to the design of highly efficient hybrid electrocatalysts for hydrogen production.
A hybrid hydrogen evolution reaction catalyst is fabricated by decorating TiO2 nanotube arrays with crystalline Ru0.33Se nanoparticles. Owing to the merits of the two components and the synergistic effect at the interface, the hybrid catalyst demonstrates an outstanding performance with a small overpotential of 57 mV to acquire a current density of 10 mA cm−2.
Long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) is an important lncRNA derived from the XIST gene in mammals. XIST is abnormally expressed in numerous tumors, in most of which XIST ...functions as an oncogene. XIST is involved in multiple aspects of carcinogenesis, including tumor onset, progression, and prognosis. In our review, we collected and analyzed the recent studies on the impact of XIST in human tumor development. The multilevel molecular functions of XIST in human tumors are comprehensively reviewed to clarify the pathologic mechanisms and to offer a novel direction for further study.
Abstract
Long noncoding RNAs are involved in epigenetic gene modification, including binding to the chromatin rearrangement complex in pre-transcriptional regulation and to gene promoters in gene ...expression regulation, as well as acting as microRNA sponges to control messenger RNA levels in post-transcriptional regulation. An increasing number of studies have found that long noncoding RNA plasmacytoma variant translocation 1 (PVT1) plays an important role in cancer development. In this review of a large number of studies on PVT1, we found that PVT1 is closely related to tumor onset, proliferation, invasion, epithelial–mesenchymal transformation, and apoptosis, as well as poor prognosis and radiotherapy and chemotherapy resistance in some cancers. This review comprehensively describes PVT1 expression in various cancers and presents novel approaches to the diagnosis and treatment of cancer.
The magnetic field response of the transport properties of novel materials and then the large magnetoresistance effects are of broad importance in both science and application. We report large ...transverse magnetoreistance (the magnetoresistant ratio ~ 1.3 × 10(5)% in 2 K and 9 T field, and 4.3 × 10(6)% in 0.4 K and 32 T field, without saturation) and field-induced metal-semiconductor-like transition, in NbSb2 single crystal. Magnetoresistance is significantly suppressed but the metal-semiconductor-like transition persists when the current is along the ac-plane. The sign reversal of the Hall resistivity and Seebeck coefficient in the field, plus the electronic structure reveal the coexistence of a small number of holes with very high mobility and a large number of electrons with low mobility. The large MR is attributed to the change of the Fermi surface induced by the magnetic field which is related to the Dirac-like point, in addition to orbital MR expected for high mobility metals.
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