Introduction
The aim of this study was to investigate how peritoneal dialysis and other influencing factors affect patients' cognitive function.
Methods
The 85 subjects in the study group were ...regular patients in our center. The control group included 88 age and gender matched healthy individuals who were with normal renal function. The study subjects' cognitive levels and related factors were analyzed using several screening instruments: the cognitive function was measured using the Montreal Cognitive Assessment Scale and statistical analysis was conducted based on the relevant data.
Results
The results showed that cognitive impairment was higher in peritoneal dialysis patients than in non‐dialysis subjects. Age and educational background were single factors that affected cognitive function, which was more likely to be impaired at a higher age level and/or a lower educational level.
Conclusion
Cognitive function can be impaired by peritoneal dialysis, and age and education levels are influencing factors.
Providing precise adjustable strategy with the properties of uniform hollow sphere MOF precursor to expose highly dispersed Cu-O-Ce active interface to enhance CO-PROX.
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•The ...quantitative Cu active species can be regulated by the loading layer of MOFs.•The hollow structure avoids the active Cu-O-Ce interface being coated inside of the catalyst.•Kinetic calculations revealed CuO/CeO2-S excellent performance for CO-PROX.•The catalytic mechanism of CuO/CeO2-S is unveiled by in-situ DRIFTs and Raman.
Derivation of CuO/CeO2 catalyst from metal-organic framework (MOF) precursor is able to facilitate the dispersion of active sites, resulting in an enhanced catalytic performance for preferential CO oxidation in H2-rich stream (CO-PROX). However, parts of the Cu-O-Ce active interfaces are usually sintered inside the catalyst, which will reduce the catalytic performance. To solve such problem and fully expose the catalytic active sites, herein, we design and synthesize a hollow spherical CeO2 supporting Cu3(BTC)2 precursor shell and then pyrolyze it to generate CuO/CeO2-S catalyst. The results demonstrated by the various characterizations such as PXRD, TEM, H2-TPR, O2-TPD, Raman and XPS are as follows: The quantitative Cu active species are achieved by controlling the loading layer of MOFs; The hollow structure can avoid the active Cu-O-Ce interface being coated inside; The CeO2-S provides the lowest loading energy of Cu and more oxygen vacancy. Further in-situ analysis of Raman and DRIFTs as well as kinetic detects exhibit the highest interfacial active Cu species ratio and Ce3+ concentration of the CuO/CeO2-S, comparing to catalysts with diverse morphologies and traditional impregnation method from MOF precursors. The as-synthesized CuO/CeO2-S-10-700 displays nearly 100% CO conversion at ca. 80 °C and outstanding stability for CO-PROX.
The primary objectives were to determine the prevalence of and identify variables associated with respiratory bacterial co-infection in COVID-19 inpatients. Secondary outcomes included length of stay ...and in-hospital mortality. Eighty-two (11.2%) of 735 COVID-19 inpatients had respiratory bacterial co-infection. Fifty-seven patients met inclusion criteria and were matched to three patients lacking co-infection (N = 228 patients). Patients with co-infection were more likely to receive antibiotics 57 (100%) vs 130 (76%), P < 0.0001 and for a longer duration 19 (13-33) vs 8 (4-13) days, P < 0.0001. The multi-variable logistic regression model revealed risk factors of respiratory bacterial co-infection to be admission from SNF/LTAC/NH (AOR 6.8, 95% CI 2.6-18.2), severe COVID-19 (AOR 3.03, 95% CI 0.78-11.9), and leukocytosis (AOR 3.03, 95% CI 0.99-1.16). Although respiratory bacterial co-infection is rare in COVID-19 inpatients, antibiotic use is common. Early recognition of respiratory bacterial coinfection predictors in COVID-19 inpatients may improve empiric antibiotic prescribing.
The proband with congenital heart disease and abnormal thumb was clinically diagnosed as Holt–Oram syndrome (HOS). A novel variant, T‐box transcription factor 5 (TBX5) c.755 + 1 G > A, was identified ...in the proband via whole exome sequencing and validated using Sanger sequencing. Pedigree analysis and clinical examinations revealed three/seven individuals over three generations within the family, with features suggestive of HOS. Deep amplicon sequencing confirmed that the allele frequencies of the novel variant in the proband (III‐1), her brother (III‐2), and her mother (II‐2) were 50%, 48.3%, and 38.1%, respectively, indicating that III‐1 and III‐2 harbored heterozygous variants, while II‐2 harbored mosaic heterozygous variants. The minigene splicing assay showed that the novel variant affected the normal splicing of exon 7, resulting in the production of abnormal TBX5 transcripts. Reverse transcription‐quantitative polymerase chain reaction and western blot analyses revealed that the novel variant upregulated TBX5 expression at the transcriptional and translational levels. Nuclear localization assay demonstrated impaired nuclear localization of the mutant TBX5. Cell viability assay revealed the inhibition of cell activity by the mutant TBX5. Our findings indicate that the novel variant was potentially induced HOS, probably by causing aberrant splicing, reducing the enrichment of nuclear TBX5 protein, and inhibiting cellular proliferation.
Though the great success of paclitaxel, the variable response of patients to the drug limits its clinical utility and the precise mechanisms underlying the variable response to paclitaxel remain ...largely unknown. This study aims to verify the role and the underlying mechanisms of CD147 in paclitaxel resistance. Immunostaining was used to analyze human non-small-cell lung cancer (NSCLC) and ovarian cancer tissues. RNA-sequencing was used to identify downstream effectors. Annexin V-FITC/propidium iodide and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were used to detect apoptosis. Co-immunoprecipitation (Co-IP), fluorescence resonance energy transfer (FRET) and surface plasmon resonance (SPR) were performed to determine protein interactions. Fluorescence recovery after photobleaching (FRAP) was performed to measure the speed of microtubule turnover. Xenograft tumor model was established to evaluate sensitivity of cancer cells to paclitaxel in vivo. In vitro and in vivo assays showed that silencing CD147 sensitized the cancer cells to paclitaxel treatment. CD147 protected cancer cells from paclitaxel-induced caspase-3 mediated apoptosis regardless of p53 status. Truncation analysis showed that the intracellular domain of CD147 (CD147
) was indispensable for CD147-regulated sensitivity to paclitaxel. Via screening the interacting proteins of CD147
, Ran binding protein 1 (RanBP1) was identified to interact with CD147
via its C-terminal tail. Furthermore, we showed that RanBP1 mediated CD147-regulated microtubule stability and dynamics as well as response to paclitaxel treatment. These results demonstrated that CD147 regulated paclitaxel response by interacting with the C-terminal tail of RanBP1 and targeting CD147 may be a promising strategy for preventing paclitaxel resistant.
Companion animals have been shown to carry Clostridioides difficile strains that are similar or identical to strains found in people, and a small number of studies have shown that pets carry ...genetically identical C. difficile isolates as their owners, suggesting inter‐species transmission. However, the directionality of transmission is ultimately unknown, and the frequency with which animals acquire C. difficile following their owners' infection is unclear. The goal of this study was to assess how often pets belonging to people with C. difficile infection carry genetically related C. difficile isolates. We enrolled pet owners from two medical institutions (University of Pennsylvania Health System (UPHS) and The Ohio State University Wexner Medical Center (OSUWMC)) who had diarrhoea with or without positive C. difficile assays and tested their faeces and their pets' faeces for C. difficile using both anaerobic culture and PCR assays. When microorganisms were obtained from both the owner and pet and had the same toxin profile or ribotype, isolates underwent genomic sequencing. Faecal samples were obtained from a total of 59 humans, 72 dogs and 9 cats, representing 47 complete households (i.e. where a sample was available from the owner and at least one pet). Of these, C. difficile was detected in 30 humans, 10 dogs and 0 cats. There were only two households where C. difficile was detected in both the owner and pet. In one of these households, the C. difficile isolates were of different toxin profiles/ribotypes (A+/B+ / RT 499 from the owner, A‐/B‐ / RT PR22386 from the dog). In the other household, the isolates were genetically identical (one SNP difference). Interestingly, the dog from this household had recently received a course of antibiotics (cefpodoxime and metronidazole). Our findings suggest that inter‐species transmission of C. difficile occurs infrequently in households with human C. difficile infections.
Several ansamycins have been reported to inhibit bacterial biofilm formation and accelerate the eradication of developed biofilms, but little is known about the effect of hygrocin C, an ansamycin, on ...bacterial biofilm formation. Here, hygrocin C was isolated from the marine-derived
Streptomyces
sp. SCSGAA 0027 and reported for the first time to be capable of inhibiting the biofilm formation of
Staphylococcus aureus
and
Bacillus amyloliquefaciens
SCSGAB0082 with the production of anti-microbial lipopeptides from South China Sea gorgonian
Subergorgia suberosa
at concentrations of less than minimum inhibitory concentrations. Moreover, hygrocin C also promoted the eradication of developed biofilms, affected the biofilm architecture, and lowered the extracellular polymeric matrix formation, cell motility, and surface hydrophobicity in
B
.
amyloliquefaciens
, which was in accordance with the inhibition of biofilm formation. Furthermore, transcriptome analysis revealed that hygrocin C altered the transcripts of several genes associated with bacterial chemotaxis and flagellar, two-component system and the synthesis of arginine and histidine, which are important for bacterial biofilm formation. In conclusion, hygrocin C could be used as a potential biofilm inhibitor against
S
.
aureus
and
B
.
amyloliquefaciens
. But further genetic investigations are needed to provide more details for elucidation of the molecular mechanisms responsible for the effects of hygrocin C on
B
.
amyloliquefaciens
biofilm formation.
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Mesoporous carbon-coated iron oxide (α-Fe2O3@C) nanoparticles were hydrothermally synthesized by using α-Fe2O3 nanospheres (ca. 50 nm) as the cores and resorcinol as the carbon source ...of the shells. The formation of the mesoporous carbon coatings was confirmed by means of SEM, TEM, FT-IR, TG, XPS and BET measurements. Moreover, the coating thickness was easily adjusted to be 4, 7 and 10 nm by changing the amount of resorcinol. When used as the anodes for lithium-ion batteries (LIBs), the α-Fe2O3@C nanoparticles exhibited significantly improved electrochemical properties as compared to pure α-Fe2O3 nanoparticles. With the increase of the coating thickness, the α-Fe2O3@C electrodes presented an initial increase and subsequent decrease in their cycling stability and rate performance. Especially, the α-Fe2O3@C-7 nanoparticles displayed a high reversible capacity of 1223 mA h/g at 1 A/g, a good cycling stability retaining 1093 mA h/g at 1 A/g after 100 cycles as well as a remarkable rate performance of 570 mA h/g at 5 A/g. The quantitative kinetic analysis revealed that 58 % of the charge storage was contributed by the capacitive process at 0.5 mV/s. The well-defined core-shell structure and superior electrochemical performance of the α-Fe2O3@C-7 nanoparticles will make them a promising anode for LIBs.
A LaNiO3 perovskite catalyst was prepared using the coprecipitation–oxidation hydrothermal method, followed by calcination at 600 °C for 2 h. The as-prepared sample was composed of La(OH)3 in nanorod ...structures and was covered with poorly crystalline Ni(OH)2. The mixed metal hydroxides were converted into cubic LaNiO3 perovskite after calcination at 600 °C. A catalytic steam reforming of ethanol (SRE) reaction for hydrogen production was performed in a fixed-bed reactor. The catalyst was reduced in situ in hydrogen at 400 °C prior to the reaction. The ethanol conversion reached 100% at 300 °C with 70% hydrogen selectivity. The highly catalytic activity of the reduced catalyst was due to the well-dispersion of Ni particles on the surface of active catalyst was formed in the in situ reduced catalyst. After a 80 h time-on-stream test at 350 °C, the used catalyst presented a La2O2CO3 component that was formed owing to the reaction of the CO2 product with La2O3. La2O2CO3 acted as a carbon reservoir to eliminate the deposited carbon and further stabilized the Ni particles on the La2O3 surface, which resulted in the highly catalytic activity during the entire reaction period. The deposited carbon after the SRE reaction was further examined by TGA, TPR, elemental analysis, and TEM.
► The catalyst was prepared by the coprecipitation–oxidation hydrothermal method. ► The ethanol conversion reached 100% at 300 °C with 70% hydrogen selectivity. ► The ethanol conversion was 100% and maintained through the 80 h test at 350 °C ► The La2O2CO3 formed acted as a carbon reservoir to eliminate the deposited carbon. ► The SMSI could stabilize the Ni particles on the La2O3 surface.
Mycobacterium tuberculosis (Mtb) and SARS-CoV-2 (CoV2) are the leading causes of death due to infectious disease. Although Mtb and CoV2 both cause serious and sometimes fatal respiratory infections, ...the effect of Mtb infection and its associated immune response on secondary infection with CoV2 is unknown. To address this question we applied two mouse models of COVID19, using mice which were chronically infected with Mtb. In both model systems, Mtb-infected mice were resistant to the pathological consequences of secondary CoV2 infection, and CoV2 infection did not affect Mtb burdens. Single cell RNA sequencing of coinfected and monoinfected lungs demonstrated the resistance of Mtb-infected mice is associated with expansion of T and B cell subsets upon viral challenge. Collectively, these data demonstrate that Mtb infection conditions the lung environment in a manner that is not conducive to CoV2 survival.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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